Vitamin D-binding protein (DBP) is an abundant serum protein, secreted by the liver, which transports vitamin D sterols and is part of an actin scavenging system. In this study, DBP was isolated from horse plasma in a highly reproducible, four step procedure: Affi-gel Blue affinity chromatography, gel filtration, hydroxy1apatite chromatography and anion exchange HPLC. 6-7 mg of DBP were obtained from 80 ml of plasma with a yield of 21-25%. The secondary structure of DBP was calculated from circular dichroism measurements to be 39% α-helix, 42% β-sheet and 19% random coil. A molecular mass of 53,000 ± 3,000 daltons was calculated from electrophoretic gels. Circular dichroism and fluorescence studies revealed that the disulphide bonds of DBP contribute substantial structural stabilization to the molecule with respect to thermal denaturation. Finally, acrylodan-labeled DBP was prepared. The fluorescence of this adduct was sensitive to the binding of actin and to the presence of dithiothreitol. / Science, Faculty of / Chemistry, Department of / Graduate

by Xiao-Ming Chen. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 127-135). / Acknowledgements / Abstract --- p.i / Nomenclature --- p.ii / Table of Contents --- p.iii / List of Tables --- p.iv-v / List of Figures --- p.v-vii / Chapter 1. --- Introduction --- p.1-9 / Chapter 2. --- Results and Discussion --- p.10-104 / Chapter 1. --- Betaine derivatives --- p.10-19 / Chapter 2. --- "Complexes of manganese(II), cobalt(II), and nickel(II)" --- p.20-31 / Chapter 3. --- Complexes of zinc(II) --- p.32-40 / Chapter 4. --- Complexes of cadmium(II) and mercury(II) --- p.41-56 / Chapter 5. --- Complexes of silver(I) --- p.57-76 / Chapter 6. --- Complexes of copper(II) --- p.77-97 / Chapter 7. --- Mixed-metal complexes --- p.98-104 / Chapter 3. --- Conclusions --- p.105-106 / Chapter 4. --- Experimental --- p.107-126 / Chapter 5. --- References --- p.127-135 / Chapter 6. --- Appendix A --- p.136-151 / Chapter 1. --- Atomic coordinates and equivalent isotropic thermal parameters --- p.136-144 / Chapter 2. --- Anisotropic thermal parameters --- p.145-151 / Chapter 7. --- Appendix B (in a separated volume to be kept as Supplementary Data in the Department of Chemistry) Tables of structure factors

"January 2002." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references. / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 111-120). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 111-120). Also available on the Internet.

Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (leaves 78-83).

The problematics of the teaching and learning of chemical bonding has been widely reported in the literature. Following this, Taber (2001) proposed a teaching sequence alternative to those available in existing curriculum documents and textbooks. He proposed that teaching be started from metallic structure to ionic structure, then giant covalent structure and finally simple molecular structure. During teaching, teachers should be cautioned about the use of language, the undue emphasis on the role of atoms (atomic ontology), the explication of the nature of bonding (based on electrostatic forces rather than electron transfer or sharing), and a better integration of the relationship between bonding and structure. In relation to the literature about scientific modelling, bonding should be taught as an ‘explanation’ of properties of chemicals, rather than a mere ‘description’ of structure. This paper reports an empirical study of a class of Year 10 students’ learning outcomes based on the proposed teaching sequence and emphases. Throughout the learning of the topic ‘chemical bonding’ which spanned two months, three students ranged from the highest to medium achieving students in the class were selected for interviews. Each student was interviewed on four separate occasions in the course of their learning of ‘chemical bonding’. Their understanding of concepts in relation to chemical bonding was probed through their verbal and visual representations. The effectiveness of the teaching sequence was evaluated in the light the alternative conceptions reported in the literature, strategy and suggestions for further improvements were discussed. / published_or_final_version / Education / Master / Master of Education

No description available.

Licentiatavhandling (sammanfattning) Uppsala : Sveriges lantbruksuniverstet, 2006. / Härtill 2 uppsatser.

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2007. / Härtill 4 uppsatser.