Thesis (Ph. D.)--University of Wisconsin--Madison, 1962. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 84-85).

No description available.

Thesis (Ph. D.)-- University of Adelaide, Dept. of Zoology, 1987. / Bibliography: leaves 214-235.

Thesis (doctoral)--Ludwig-Maximilians-Universität München, 2006. / Title from PDF title page (viewed on May 13, 2006). Vita. Includes bibliographical references (p. 253-264).

Made available in DSpace on 2016-06-02T19:28:56Z (GMT). No. of bitstreams: 1 TeseLCSC.pdf: 8965272 bytes, checksum: 0c3270a33d0c6e8fb79427159bec4c82 (MD5) Previous issue date: 2004-04-30 / Universidade Federal de Minas Gerais / The present work aimed to study one of the most used genera in water quality assessment, Chironomus, identifying and/or describing the species inhabiting aquatic systems of the State of São Paulo with varied ecological features. The study area included 27 aquatic systems consisting of lentic habitats (lakes, reservoirs, temporary pools, waste stabilization lagoons and fish ponds) and lotic habitats (streams, rivers and drainage channels) at different localities of Mogi-Guaçu and Tietê-Jacaré hydrographic sub-basins. The sampling and analyses followed the conventional methods used in taxonomic and limnological studies. The survey resulted in the identification of 16 Chironomus species, including 7 new records to the State of São Paulo and 8 new species. Besides C. sancticaroli Strixino & Strixino, 1981, a species previously recorded in the State of São Paulo, I identified the immatures and adults of C. calligraphus Goeldi, 1905, C. gigas Reiss, 1974, C. latistylus Reiss, 1974, C. paragigas Reiss, 1974, C. strenzkei Fittkau, 1968, C. stigmaterus Say, 1823 and C. columbiensis Wülker et al., 1989. Some species were sampled in similar habitats, indicating a possible relation with them. C. gigas, C. paragigas and C. latistylus were collected only in lentic systems with low antropic influence. The immatures of C. sp. 1, C. sp. 5 and C. sp. 6 were sampled only in association with aquatic macrophytes growing in lakes and stream pools. C. sp. 3 and C. sp. 4 inhabited temporary pools with great amounts of detritus. C. stigmaterus and C. calligraphus were found only in waste stabilization lagoons. Only the larvae of C. sp. 2 were sampled in lotic systems polluted with high level of organic material from urban sewage and agricultural activities. The morphological analyses of larvae enabled the construction of a preliminary identification key for the species of Chironomus that live in the different aquatic systems of the State of São Paulo. This key could be a useful tool in analyses and diagnostics carried out during water quality assessment programs. / O objetivo deste trabalho foi estudar um dos gêneros mais utilizados em avaliações da qualidade da água, Chironomus, identificando e/ou descrevendo as espécies existentes em ambientes aquáticos do Estado de São Paulo com diferentes características ecológicas. A área de estudo compreendeu 27 sistemas incluindo ambientes lênticos (lagoas, represas, poças temporárias, lagoas de estabilização, viveiros) e lóticos (córregos, rios e canais de drenagem) de diferentes localidades das sub-bacias hidrográficas Mogi-Guaçu e Tietê-Jacaré. As coletas e análises seguiram a metodologia convencional para estudos limnológicos e taxonômicos. Nos sistemas analisados foram identificadas 16 espécies de Chironomus, das quais 7 são novos registros para o Estado de São Paulo e 8 são novas espécies. Além de C. sancticaroli Strixino & Strixino, 1981, espécie já registrada no estado de São Paulo, foram identificados imaturos e adultos de C. calligraphus Goeldi, 1905, C. gigas Reiss, 1974, C. latistylus Reiss, 1974, C. paragigas Reiss, 1974, C. strenzkei Fittkau, 1968, C. stigmaterus Say, 1823 e C. columbiensis Wülker et al., 1989. Algumas espécies foram amostradas em ambientes com características semelhantes, apontando uma possível relação com aqueles sistemas. C. gigas, C. paragigas e C. latistylus foram coletadas apenas em ambientes lênticos com reduzidos impactos antrópicos. Os imaturos de C. sp. 1, C. sp. 5 e C. sp. 6 foram obtidos apenas junto a macrófitas aquáticas que se desenvolvem em lagoas ou em remansos de córregos. C. sp. 3 e C. sp. 4 estiveram presentes em águas temporárias com grande quantidade de detritos vegetais. C. stigmaterus e C. calligraphus só foram amostrados em lagoas de estabilização. As larvas de C. sp. 2 foram as únicas amostradas em vários sistemas lóticos que recebem elevadas cargas orgânicas provenientes de esgoto urbano e de atividades agrícolas. A análise morfológica das larvas possibilitou a construção de uma chave preliminar de identificação das espécies do gênero que vivem nos diferentes sistemas aquáticos do Estado de São Paulo. Esta poderá servir como ferramenta para as análises e diagnósticos realizados em programas de avaliação da qualidade da água.

A preliminary cytotaxonomic study of the common Chironomidae in a series of saline waters in the southern interior of British Columbia has been undertaken. The banding pattern of the salivary gland chromosomes, once it had been described, was used as a taxonomic criterion and as an indicator of the relationships between the groups involved. In order to obtain associated stages in the life cycle, the larvae were reared in individual vials. The polytene chromosome analysis revealed seven well defined larval species. The subsequent morphological analysis showed that five of these larval species could usually be separated by their external morphology. However, two cytologically distinct species are morphologically indistinguishable. Recent work on sibling species in Drosophila and Chironomus (Diptera) has shown that sibling species have salivary gland chromosomes with an identical banding pattern but, differ from one another in the frequency of inversions. The present study suggests that the morphologically identical larvae mentioned above are sibling species in larval morphology but are clearly separable by chromosome analysis. The fact that they occur together in the same lake tends to eliminate the view that they are distinct populations of a single species. That there may be more than one species involved in those here considered to be a single taxa, should however not be forgotten. / Science, Faculty of / Zoology, Department of / Graduate

This thesis deals with the importance of salinity to the distribution of three chironomid species of the genus Chironomus (C. anthracinus. C. athalassicus. and C. tentans). Research to date suggests that salinity and coexistence problems are the major factors influencing the distribution of the chironomid fauna of fresh and saline lakes in the Cariboo and Chilcotin areas of central British Columbia. The difference in the distribution of these three Chironomus species is particularly interesting. The investigation of the importance of salinity to their distribution consisted of a study of (i) the salinity tolerance of each species, (ii)the regulation of the haemolymph, and (iii) the influx and efflux of sodium and chloride ions. Several conclusions were drawn from the investigation. A difference in the salinity tolerance of each species was found in the laboratory: C. anthracinus and C. tentans did not survive at lake water conductivities above 9000 micro-o mhos/cm at 25 C, C. athalassicus survived in lake water conductivities at least as high as 15»000 micromhos/cm at o 25 C. Temperature affected the survival of each species in a similar way; at high temperatures survival time decreased. Sodium, potassium, and chloride as well as the concentration of the haemolymph were regulated by the three species at low salinities. C. athalassicus was the only species able to conform at higher external concentrations. C. athalassicus had a low sodium affinity and a powerful sodium uptake system compared to the other species. Chloride affinity and the power of the uptake system exceeded that of C. anthracinus and C. tentans. The general conclusion reached was that salinity does affect the distribution of the three Chlronomus species. More research is called for in this and related areas in order to more fully understand the distribution of the chironomid fauna. / Science, Faculty of / Zoology, Department of / Graduate

Made available in DSpace on 2016-06-02T19:26:19Z (GMT). No. of bitstreams: 1 BEGHELLI_Frederico_2011.pdf: 1917352 bytes, checksum: 6e96950126e3e035b5f202756ee4463c (MD5) Previous issue date: 2011-03-14 / Universidade Federal de Minas Gerais / The objectives of this work were the benthic macroinvertebrate community characterization from Itupararanga Reservoir, High Sorocaba Basin, SP, Brazil and the identification of the main abiotic factors wich affects the structure and distribution of those organisms. Samplings were taken with Van-Veen grab three times (cumulative samplings). The samplings were taken at three reservoir‟s zones: riverine zone, transitional and lacustrine ones and at two regions: litoral and central ones. Furthermore, it was taken samples during the wet (December 2009 and February 2010) and dry seasons (June and August 2010). The dissolved oxygen concentrations, electric conductivity, pH, transparency and depth near the bottom were reccorded in situ by using a multiprobe and Secchi disc in all sample points. With the objective to verify witch environmental variables would be the most important for the benthic macroinvertebrates community characterization, a correspondence canonical analisis (CCA) was done by the main taxa density logarithm and the water and sediment variables data matrix. The total number of reccorded taxa was 28. The Diptera family Chironomidae was the richest group (19 taxa). The Tubificinae (Clitellata, Naididae) were numerically dominants during the wet season and the Chironomidae (Diptera) were it during the dry one. Similarity analysis was performed in order to test the spatial heterogeneity hypotesis even in the longitudinal either in the transversal directions and the seasonly effects over the community. The results points to the occurrence of the spatial heterogeneity in both directions. So the reservoir diverges either in riverine, transitional and lacustrine zones, and central and litoral regions. The riverine zone is more distinctive than the others. This zone is characterized by having high nutrient concentrations (even in the water, either in the sediment) and coarse sediment. Moreover, the characteristic taxa are: Limnodrillus hoffmeisteri, Branchiura sowerbyi and Chironomus sp. It has high density values and dominance when compared to the other zones. The transitional and lacustrine zones presents some more intense transversal heterogeneity with more rich and diverse communities near the margins. It was noticed that the benthic macroinvertebrates responses to spatial gradient of environmental conditions in both senses, the longitudinal, and the transversal. Factors associated to environmental impacts and natural factors like granulometric sediment composition or depth can be determinants to the structure and composition of the community. The Itupararanga Reservoir is in eutrophication process and the riverine zone is the most affected area because it receives more directly the possible headwater impacts / Os objetivos deste trabalho foram a caracterização da comunidade de macroinvertebrados bentônicos da Represa de Itupararanga, Bacia do Alto Sorocaba, SP e a identificação de fatores abióticos determinantes da estrutura e distribuição destes organismos. Foram realizadas coletas com draga do tipo Van-Veen em triplicada (amostras cumulativas). As coletas foram realizadas em três zonas distintas da represa: zona de rio, zona de transição e zona de lago e em duas regiões: litoral e central. Além disso, foram realizadas duas coletas durante a estação chuvosa (dezembro de 2009 e fevereiro de 2010) e duas durante a estação seca (junho e agosto de 2010). Foram realizadas análises in situ de teor de oxigênio dissolvido, condutividade elétrica, temperatura, pH, transparência e profundidade d‟água próxima ao sedimento com a utilização de multisonda e disco de Secchi em todos os pontos amostrais. Em laboratório, foram determinadas as concentrações de fósforo total e clorofila a da subsuperfície a fim de se calcular o índice de estado trófico, em cada zona de amostragem. Com relação ao sedimento, foram determinados o teor de matéria orgânica, concentrações de fósforo e nitrogênio totais e composição granulométrica para todos os pontos amostrais. Com o objetivo de se verificar quais variáveis ambientais seriam mais importantes na caracterização da comunidade de macroinvertebrados bentônicos, foi realizada uma análise de correspondência canônica (ACC) a partir da matriz de dados da água e do sedimento e logarítimo da densidade de organismos dos principais táxons. No total foram registrados 28 táxons. A família de Diptera Chironomidae foi o grupo responsável pela maior riqueza (19 táxons). Os Tubificinae (Clitellata, Naididae) foram numericamente dominantes durante a estação chuvosa e os Chironomidae (Diptera) durante a estação seca. Foram realizadas análises de similaridade para se testar a hipótese de heterogeneidade espacial tanto no sentido transversal quanto longitudinal e efeitos da sazonalidade sobre as comunidades. Os resultados indicam haver a heterogeneidade espacial diferenciando a represa em zonas de rio, de transição e de lago, bem como em região litoral e central. A zona de rio diferencia-se mais intensamente das demais. Esta zona é caracterizada por maiores concentrações de nutrientes (no sedimento e na água) bem como por possuir sedimento mais grosseiro. Além disso, apresenta como táxons característicos Limnodrillus hoffmeisteri, Branchiura sowerbyi e Chironomus sp. Também caracteriza-se por elevados valores de densidade quando em comparação com os demais pontos amostrais e elevada dominância. As zonas de transição e lago apresentaram forte diferenciação transversal com comunidades mais ricas e com maior diversidade próximas às margens. Observou-se que os macroinvertebrados bentônicos respondem a um gradiente espacial de condições ambientais tanto no sentido longitudinal quanto transversal sendo que tanto fatores relacionados à impactos no ambiente quanto fatores naturais como composição granulométrica e profundidade podem ser determinantes na composição e estrutura da comunidade. A Represa de Itupararanga encontra-se em processo de eutrofização sendo que a zona de rio é a área mais afetada por receber diretamente os possíveis impactos a montante.

by Yuen Wai Keung. / Thesis submitted in: December 1999. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 99-112). / Abstracts in English and Chinese. / Acknowledgement --- p.i / Abstract --- p.ii / Abstract (Chinese Version) --- p.iv / Abbreviations --- p.vi / Table of Contents --- p.viii / Chapter chapter one --- introduction --- p.1 / Chapter 1.1 --- Glutathione S-transferase --- p.2 / Chapter 1.1.1 --- Introduction --- p.2 / Chapter 1.1.2 --- Classification of mammalian GST --- p.2 / Chapter 1.1.3 --- Classification of insect GST --- p.7 / Chapter 1.1.4 --- Substrate specificity --- p.11 / Chapter 1.2 --- The chironomidae --- p.13 / Chapter 1.2.1 --- Biology and life history of chironomidae --- p.13 / Chapter 1.3 --- Chironomidae larvae --- p.16 / Chapter 1.3.1 --- Bloodworm t --- p.6 / Chapter 1.3.2 --- Sources of chironomidae larvae --- p.17 / Chapter 1.4 --- Aim of research --- p.18 / Chapter chapter two --- materials and methods --- p.20 / Chapter 2.1 --- Screening of GST in different subcellular fractions --- p.21 / Chapter 2.1.1 --- Preparation of mitochondria --- p.21 / Chapter 2.1.2 --- Preparation of microsomes --- p.22 / Chapter 2.1.3 --- Preparation of cytosol --- p.22 / Chapter 2.2 --- Assay for GST activity --- p.23 / Chapter 2.2.1 --- Activity Units --- p.23 / Chapter 2.3 --- Protein assay --- p.23 / Chapter 2.4 --- Preparation of glutathione-affinity column --- p.25 / Chapter 2.5 --- Purification of cytosolic GSTs --- p.26 / Chapter 2.5.1 --- Preparation of cytosol --- p.26 / Chapter 2.5.2 --- Chromatography on Sephadex G25 --- p.26 / Chapter 2.5.3 --- Affinity Chromatography --- p.26 / Chapter 2.5.3.1 --- Specific elution of GSTs --- p.26 / Chapter 2.5.3.2 --- Non-specific elution of GSTs --- p.27 / Chapter 2.5.4 --- Fast Protein Liquid Chromatography with Mono Q --- p.27 / Chapter 2.6 --- Determination of molecular mass --- p.29 / Chapter 2.6.1 --- Subunit molecular mass --- p.29 / Chapter 2.6.2 --- Native molecular mass --- p.31 / Chapter 2.7 --- Isoelectric focusing PAGE --- p.31 / Chapter 2.8 --- Enzyme activities and kinetic studies --- p.34 / Chapter 2.8.1 --- Optimum pH --- p.34 / Chapter 2.8.2 --- Heat inactivation assay --- p.34 / Chapter 2.8.3 --- Km and Vmax --- p.34 / Chapter 2.8.4 --- Substrate specificity --- p.35 / Chapter 2.8.5 --- Glutathione peroxidase activity --- p.38 / Chapter 2.9 --- N-terminal amino acid sequence analysis --- p.39 / Chapter 2.9.1 --- Semidry electroblotting --- p.39 / Chapter 2.9.2 --- Staining of proteins on PVDF membrane --- p.40 / Chapter 2.9.3 --- N-terminal amino acid sequence analysis --- p.40 / Chapter 2.9.4 --- On-membrane deblocking of protein --- p.40 / Chapter 2.9.5 --- BLAST search --- p.41 / Chapter chapter three --- results --- p.42 / Chapter 3.1 --- Screening of GST in different subcellular fractions --- p.43 / Chapter 3.2 --- Purification of cytosolic GSTs by chromatography --- p.45 / Chapter 3.2.1 --- Sephadex G25 column --- p.45 / Chapter 3.2.2 --- GSH affinity column --- p.45 / Chapter 3.2.3 --- Mono-Q column --- p.45 / Chapter 3.3 --- Determination of molecular mass --- p.53 / Chapter 3.3.1 --- Subunit molecular mass --- p.53 / Chapter 3.3.2 --- Native molecular mass --- p.53 / Chapter 3.4 --- Isoelectric point determination --- p.53 / Chapter 3.5 --- Enzymes activities and kinetic studies --- p.57 / Chapter 3.5.1 --- Optimum pH --- p.57 / Chapter 3.5.2 --- Thermostability --- p.57 / Chapter 3.5.3 --- Km and Vmax --- p.57 / Chapter 3.5.4 --- Substrate specificity --- p.76 / Chapter 3.5.5 --- Glutathione peroxidase Activity --- p.76 / Chapter 3.6 --- N-terminal amino acid sequence analysis --- p.83 / Chapter chapter four --- discussion --- p.89 / Chapter 4.1 --- GST in different subcellular fractions --- p.90 / Chapter 4.2 --- Purification of cytosolic GST --- p.91 / Chapter 4.3 --- Physical properties --- p.93 / Chapter 4.3.1 --- Subunit molecular mass --- p.93 / Chapter 4.3.2 --- Native molecular mass --- p.93 / Chapter 4.3.3 --- Isoelectric point --- p.95 / Chapter 4.4 --- Kinetic properties --- p.94 / Chapter 4.4.1 --- Optimum pH --- p.94 / Chapter 4.4.2 --- Thermostability --- p.95 / Chapter 4.4.3 --- Km and Vmax --- p.95 / Chapter 4.4.4 --- Substrate specificity --- p.96 / Chapter 4.4.5 --- Glutathione peroxidase activity --- p.96 / Chapter 4.5 --- N-terminal amino acid sequence data --- p.97 / Chapter 4.6 --- Conclusion --- p.98 / references --- p.99

Submitted by Dominick Jesus (dominickdejesus@hotmail.com) on 2016-01-05T20:06:07Z No. of bitstreams: 2 Tese_Galileu Petronildo da Silva Dantas.pdf: 25835498 bytes, checksum: 06db576d92df91caa4a5f56ed998692b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) / Made available in DSpace on 2016-01-05T20:06:07Z (GMT). No. of bitstreams: 2 Tese_Galileu Petronildo da Silva Dantas.pdf: 25835498 bytes, checksum: 06db576d92df91caa4a5f56ed998692b (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2015-02-27 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / The first chapter is a review of the Neotropical species of the genus Larsia. The species previously recorded for the neotropical region were redescribed and illustrated, in some cases it was not possible to analyze the material and their situations are discussed in the text. Thirteen species were described as new, and all with material from Brazil. Eight morphotypes were described, three based on males and 5 based on pupae of diferent locations in Central and South America. Keys to male, pupa and larva to those described neotropical species were prepared. The second chapter deals with the Tanypodinae subfamily in the Amazon. A total of 21 species belonging to 11 genera were analyzed, 3 of them were identified as Laurotanypus travassosi, Monopelopia caraguata and M. tenuicalcar, 2 were identified as Pentaneura, but described as morphotypes since only females were associated with immature and 14 species were described as new. Two species have been described as genus A and genus B, since it does not fit the description of any known group of Tanypodinae. Three genera, Clinotanypus, Denopelopia and Parapentaneura were recorded for the first time in the Amazon. Keys to male, pupa and larva of the genera found in the Amazon have been prepared. With these results the number of described species of Tanypodinae registered in Amazon increases from 19 to 44. / De modo geral, o presente trabalho trata de taxonomia de Tanypodinae e está dividida em dois capítulos. O primeiro capítulo é uma revisão das espécies neotropicais do gênero Larsia. As espécies previamente registradas para a região foram redescritas e ilustradas, em alguns casos não foi possível analisar o material e suas situações são discutidas no texto. Treze espécies foram descritas, todas com material do Brasil. Oito morfótipos foram descritos, três baseados em imagos e cinco, em pupas, com material proveniente da América Central e do Sul. Chaves para identificar macho, pupa e larva para as espécies neotropicais de Larsia foram elaboradas. O segundo capítulo trata da subfamília Tanypodinae na Amazônia. Foram analisadas 21 espécies distribuídas em 11 gêneros, dessas, 14 representam espécies novas, duas são espécies de Pentaneura, que foram morfotipadas uma vez que apenas fêmeas associadas a imaturos estão disponíveis, duas representam novos registros para o Amazonas (Monopelopia caraguata e M. tenuicalcar), e dois gêneros monotípicos, Gênero A e Gênero B, foram descritos como morfotipos. Três gêneros, Clinotanypus, Parapentaneura e Denopelopia, foram registrados pela primeira vez na Amazônia. Chaves para identificar macho, pupa e larva dos gêneros encontrados na Amazônia foram elaboradas. Com esses resultados o número de espécies descritas de Tanypodinae registradas na Amazônia passou de 19 para 44.