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Lipid analysis of wild type and caveolin-1 knock out mouse embryonic fibroblastsLi, Qiong, Medical Sciences, Faculty of Medicine, UNSW January 2008 (has links)
The aim of this project is to characterise the levels of cholesterol and phospholipid in cell homogenates, plasma membranes and isolated membrane domains from wildtype (WT) and caveolin-l knock-out (Cav-1-/ -) mouse embryonic fibroblasts (MEFs). Quantitative lipid analysis was developed for cholesterol by high-performance liquid chromatography (HPLC) and for glycerophospholipids (GPL) and sphingomyelin (SM) by electrospray ionisation mass spectrometry (ESI-MS). In the cell homogenates, by comparing WT to Cav-l-/- MEFs, it was found that the total cholesterol as well as the proportions of the main GPLs such as Phosphatidylcholines (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS) was similar in both cell types. However, Cav-l-/- MEFs have higher levels of cholesterol esters than WT MEFs in whole cell homogenate. Furthermore, the proportion of SM is higher in Cav-l-/- than WT MEFs. These data suggest that Cav-l may control the balance between free and esterified cholesterol and is involved in SM metabolism. A small increase in SM level in Cav-l-/- compared to WT plasma membrane was observed but this increase was not significant. Similarly, cholesterol levels in the plasma membrane are comparable between the two cell types. In both cell types, the levels of SM and phosphatidylglycerol (PG) are higher in the plasma membranes than in cell homogenates. In the WT cells, PE levels are higher in the plasma membrane than in the cell homogenates. While in the Cav-1-/- cells, the level of free cholesterol and PC are higher in the plasma membrane compared to the cell homogenate. PCs are the predominant lipids in both cell types. Cav-1-l - cells have more saturated fatty acyl chains in their PC species and shorter carbon chains compared to WT MEFs and this trend was found in both cell homogenate and plasma membranes. In PEs and SMs, Cav-1-/ - cells also have higher levels of saturated PEs and saturated amide-linkage SM than in WT cells, respectively. The results indicate that Cav-1 may also play a role in fatty acids metabolism. In summary, the data from this work indicate that Cav-l expression affects lipid composition in MEFs including the relative distribution of free and esterified cholesterol, the levels of SM relative to other phospholipid subclasses and the incorporation of fatty acids into phospholipids.
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Membraine-mediated control of [beta]-hydroxy-[beta]-methylglutaryl coenzyme A reductase in rat liverAbdullah bin Sipat January 1978 (has links)
xix, 207 leaves : ill., diagrs., graphs, tables, photo. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.1980)--The Dept. of Animal Physiology, University of Adelaide
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Nuclear receptors in control of cholesterol transportVeen, Jelske Nynke van der, January 2007 (has links)
Proefschr. Rijksuniversiteit Groningen. / Met lit.opg.-Met samenvatting in het Nederlands.
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Cholesteryl ester transfer protein its role in cardiovascular disease and drug development /Grooth, Gretha Johanna de, January 1900 (has links)
Proefschrift Universiteit van Amsterdam. / Tekst deels in het Nederlands. Met lit. opg. - Met samenvatting in het Nederlands.
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Bile acids enterohepatic circulationLi, Hai. January 2005 (has links)
Proefschrift Universiteit van Amsterdam. / Met lit. opg. - Met samenvatting in het Nederlands.
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Subcellular localization of the human isoprenoid biosynthesis pathwayHogenboom, Sietske, January 2004 (has links)
Proefschrift Universiteit van Amsterdam. / Met lit. opg. - Met samenvatting in het Nederlands.
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Cholesterol turnover in acute myelogenous leukemia with special emphasis on regulation of low density lipoprotein receptor expression in leukemic cells /Tatidis, Loukas, January 1900 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2001. / Härtill 6 uppsatser.
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Membraine-mediated control of [beta]-hydroxy-[beta]-methylglutaryl coenzyme A reductase in rat liver.Abdullah bin Sipat. January 1978 (has links) (PDF)
Thesis (Ph.D. 1980) - The Department of Animal Physiology, University of Adelaide.
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Genetic analysis of Alzheimer's disease associated genes : a perspective from abnormal cholesterol metabolism /Li, Yan, January 2008 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2008. / Also available online.
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Genetic analysis of Alzheimer's disease associated genes a perspective from abnormal cholesterol metabolism /Li, Yan, January 2008 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2008.
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