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11	Příprava a charakterizace buněčných modelů lysosomálních dědičných onemocnění - mukopolysacharidos / Preparation and characterization of cell models of lysosomal hereditary diseases - Mucopolysaccharidoses Presová, Gabriela January 2020 (has links) Mucopolysaccharidoses are a group of diseases that belong to lysosomal storage disorders. A common sign of these monogenic multisystem diseases is a gene mutation leading to a deficiency of the lysosomal enzyme participating in glycosaminoglycan degradation. It results to their accumulation in the tissues and organs, where they cause a progressive damage. There is no efficient treatment available for most mucopolysaccharidoses. Moreover, the research is complicated because of the low prevalence and type of affected tissues. Animal models of these human diseases are used for an evaluation of newly developed therapeutic approaches. However, they also have many limitations due to the different pathogenesis and catabolic pathways of the accumulated substrates between humans and animals. Therefore, animal models are replaced by human cell models. In this thesis, the development of four mucopolysaccharidoses human cell models is reported (MPS IIID, MPS IVA, MPS IVB, MPS VI). Corresponding genes (GNS, GALNS, GLB1, ARSB) were inactivated using CRISPR/Cas9 technology, where plasmids containing specific inserts are delivered to the target human induced pluripotent stem cells (iPSC), using electroporation. Isolated clones, which represent iPSC disease models, were characterized by Sanger sequencing, enzyme...
12	Proliferations- und Differenzierungsverhalten humaner Zahnkeimzellen der Pulpa / Proliferation and Differentiation Characteristics of Human Pulp Cells taken from Tooth Germs Gümmer, Andrea Mirja 15 November 2011 (has links) No description available. 610 Medizin, Gesundheit Medicine humane Pulpazellen Zahnkeim STRO-1 CD271 CD133 Proliferation multipotente Stammzellen chondrogene Differenzierung adipogene Differenzierung osteogene Differenzierung human dental pulp tooth germ STRO-1 CD271 CD133 proliferation multipotent stem cells chondrogenic differentiation adipogenic differentiation osteogenic differentiation 44.03 Methoden und Techniken der Medizin MED 280: Biologie

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Příprava a charakterizace buněčných modelů lysosomálních dědičných onemocnění - mukopolysacharidos / Preparation and characterization of cell models of lysosomal hereditary diseases - Mucopolysaccharidoses

Proliferations- und Differenzierungsverhalten humaner Zahnkeimzellen der Pulpa / Proliferation and Differentiation Characteristics of Human Pulp Cells taken from Tooth Germs