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Diagnosi e prognosi molecolare nel linfoma caninoCalzolari, Claudia <1976> 18 May 2007 (has links)
No description available.
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Quadri clinici in situazioni di: acidosi metabolica, iper L-lattacidemia e iper D-lattacidemia sperimentalmente indotte nel vitello lattanteSconza, Sarah <1976> 18 May 2007 (has links)
No description available.
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Aspetti radiografici della distribuzione dell'edema polmonare cardiogeno nel cane: ipotesi eziopatogeniche ed osservazioni personaliPivetta, Mauro <1977> 26 May 2008 (has links)
No description available.
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Valutazione dell'attività delle metalloproteinasi 2 e 9 nelle urine e nel tessuto renale di cani normali e affetti da nefropatiaMancini, Danilo <1976> 11 April 2008 (has links)
Matrix metalloproteinases (MMP) are a large family of proteinases that
remodel extracellular matrix (ECM) component. Recent data suggest a role for
MMPs in a number of renal pathophysiologies, associated with an imbalance of ECM
syntesis and degradation, which may result in an accumulation of ECM molecules
and renal fibrosis. The aim of this study is to elucidate the role of pro and activated
MMP-2 and 9 in urine and renal tissue of healty and nephropatic dogs. Renal tissue
of 8 healty dogs and either renal tissue and urine of 9 nephropatic dogs was collected
and analize using zimographic method, which is been validated in this study. Either
MMPs zimographic bands were present in almost all samples. In particular, pro and
activated MMP-9 zimographic bands were poorly represent in renal tissue of healty
dogs, whereas were very represent in nephropatic dogs. Pro and activated MMP-2
was present in either tissue of healty and nephropatic dogs. In urine of nephropatic
dogs, pro and activated MMP-9 was more evident than MMP-2, but there was not
correlaction with renal tissue levels, therefore urine levels of MMPs have poorly
usefulness in diagnostic pratice. The values of Pro and activated MMP-9 in
nephropatic dogs were significantly higher compared with normal dogs (p < 0,05),
whereas there was not statistically meaningful for Pro and activated MMP-2. In
conclusion, in this study we have validated a zimographic method for renal tissue of
dogs and we have illustrated the changes in nephropatic dogs, which may be useful
for further study.
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Importanza prognostica delle citochine e loro ruolo nell'immunomodulazione: risultati di studi sperimentali in vivo e in vitroBonato, Andrea <1980> 11 April 2008 (has links)
Citokines are proteins produced by several cell types and secreted in response to various
stimuli. These molecules are able to modify the behaviour of other cells inducing activities
like growth, differentiation and apoptosis.
In the last years, veterinary scientists have investigated the role played by these factors; in
fact, cytokines can act as intercellular communicative signals in immune response, cell
damage repair and hematopoiesis. Up to date, various cytokines have been identified and in
depth comprehension of their effects in physiology, pathology and therapy is an interesting
field of research.
This thesis aims to understand the role played by these mediators during natural or
experimentally induced pathologies. In particular, it has been evaluated the genic and protein
expressions of a large number of cytokines during several diseases and starting from different
matrix.
Considering the heterogeneity of materials used in experimentations, multiple methods and
protocols of nucleic acids and proteins extractions have been standardized.
Results on cytokines expression obtained from various in vitro and in vivo experimental
studies have shown how important these mediators are in regulation and modulation of the
host immune response also in veterinary medicine.
In particular, the analysis of inflammatory and septic markers, like cytokines, has allowed a
better understanding in the pathogenesis during horse Recurrent Airway Obstruction, foal
sepsis, Bovine Viral Diarrhea Virus infection and dog Parvovirus infection and the effects of
these agents on the host immune system.
As experimentations with mice have shown, some pathologies of the respiratory and nervous
system can be reduced or even erased by blocking cytokines inflammatory production.
The in vitro cytokines expression evaluation in cells which are in vivo involved in the
response to exogenous (like pathogens) or endogenous (as it happens during autoimmune
diseases) inflammatory stimuli could represent a model for studying citokines effects during
the host immune response. This has been analyzed using lymphocytes cultured with several
St. aureus strains isolated from bovine mastitic milk and different colostrum products. In the
first experiment different cytokines were expressed depending on enterotoxins produced,
justifying a different behaviour of the microrganism in the mammal gland. In the second one,
bone marrow cells derived incubated with murine lymphocytes with colostrum products have
shown various cluster of differentiation expression , different proliferation and a modified
cytokines profile.
A better understanding of cytokine expression mechanisms will increase the know-how on
immune response activated by several pathogen agents.
In particular, blocking the cytokine production, the inhibition or catalyzation of the receptor
binding mechanism and the modulation of signal transduction mechanism will represent a
novel therapeutic strategy in veterinary medicine.
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Studio clinico e genetico di nuove malattie ereditarie del bovinoRossi, Marco <1979> 11 April 2008 (has links)
No description available.
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Applicazione dell'esame ecocardiografico: doppler tissutale pulsato in cani di razza boxerCappi, Mario <1971> 10 March 2008 (has links)
No description available.
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Valutazione del rapporto urinario proteine totali/creatinina e albumina/creatinina in cani affetti da iperadrenocorticismo e da diabete mellitoMazzi, Antonella <1972> 11 April 2008 (has links)
INTRODUCTION – In human medicine, diabetes mellitus (DM), hypertension,
proteinuria and nephropathy are often associated although it is still not clear whether
hypertension is the consequence or the cause of nephropathy and albuminuria.
Microalbuminuria, in humans, is an early and sensitive marker which permits timely
and effective therapy in the early phase of renal damage. Conversely, in dogs, these
relationships were not fully investigated, even though hypertension has been
associated with many diseases (Bodey and Michell, 1996). In a previous study, 20%
of diabetic dogs were found proteinuric based on a U:P/C > 1 and 46% were
hypertensive; this latter finding is similar to the prevalence of hypertension in diabetic
people (40-80%) (Struble et al., 1998). In the same canine study, hypertension was
also positively correlated with the duration of the disease, as is the case in human
beings. Hypertension was also found to be a common complication of
hypercortisolism (HC) in dogs, with a prevalence which varies from 50 (Goy-Thollot
et al., 2002) to 80% (Danese and Aron, 1994).The aim of our study was to evaluate
the urinary albumin to creatinine ratio (U:A/C) in dogs affected by Diabetes Mellitus
and HC in order to ascertain if, as in human beings, it could represent an early and
more sensitive marker of renal damage than U:P/C. Furthermore, the relationship
between proteinuria and hypertension in DM and HC was also investigated.
MATERIALS AND METHODS – Twenty dogs with DM, 14 with HC and 21
healthy dogs (control group) were included in the prospective case-control study.
Inclusion criteria were hyperglycaemia, glicosuria and serum fructosamine above the
reference range for DM dogs and a positive ACTH stimulation test and/or low-dose
dexamethasone test and consistent findings of HC on abdominal ultrasonography in
HC dogs. Dogs were excluded if affected by urinary tract infections and if the serum
creatinine or urea values were above the reference range. At the moment of inclusion,
an appropriate therapy had already been instituted less than 1 month earlier in 12
diabetic dogs. The control dogs were considered healthy based on clinical exam and
clinicopathological findings. All dogs underwent urine sample collection by
cystocentesis and systemic blood pressure measurement by means of either an
oscillometric device (BP-88 Next, Colin Corporation, Japan) or by Doppler ultrasonic
traducer (Minidop ES-100VX, Hadeco, Japan). The choice of method depended on the
dog’s body weight: Doppler ultrasonography was employed in dogs < 20 kg of body
weight and the oscillometric method in the other subjects. Dogs were considered
hypertensive whenever systemic blood pressure was found ≥ 160 mmHg. The urine
was assayed for U:P/C and U:A/C (Gentilini et al., 2005). The data between groups
were compared using the Mann-Whitney U test. The reference ranges for U:P/C and
U:A/C had already been established by our laboratory as 0.6 and 0.05, respectively.
U:P/C and U:A/C findings were correlated to systemic blood pressure and Spearman
R correlation coefficients were calculated. In all cases, p < 0.05 was considered
statistically significant.
RESULTS – The mean ± sd urinary albumin concentration in the three groups was
1.79 mg/dl ± 2.18; 20.02 mg/dl ± 43.25; 52.02 mg/dl ± 98.27, in healthy, diabetic and
hypercortisolemic dogs, respectively. The urine albumin concentration differed
significantly between healthy and diabetic dogs (p = 0.008) and between healthy and
HC dogs (p = 0.011). U:A/C values ranged from 0.00 to 0.34 (mean ± sd 0.02 ± 0.07),
0.00 to 6.72 (mean ± sd 0.62 ± 1.52) and 0.00 to 5.52 (mean ± sd 1.27 ± 1.70) in the
control, DM and HC groups, respectively; U:P/C values ranged from 0.1 to 0.6 (mean
± sd 0.17 ± 0.15) 0.1 to 6.6 (mean ± sd 0.93 ± 1.15) and 0.2 to 7.1 (mean ± sd 1.90 ±
2.11) in the control, DM and HC groups, respectively. In diabetic dogs, U:A/C was
above the reference range in 11 out of 20 dogs (55%). Among these, 5/20 (25%)
showed an increase only in the U:A/C ratio while, in 6/20 (30%), both the U:P/C and
the U:A/C were abnormal. Among the latter, 4 dogs had already undergone therapy.
In subjects affected with HC, U:P/C and U:A/C were both increased in 10/14 (71%)
while in 2/14 (14%) only U:A/C was above the reference range. Overall, by
comparing U:P/C and U:A/C in the various groups, a significant increase in protein
excretion in disease-affected animals compared to healthy dogs was found. Blood
pressure (BP) in diabetic subjects ranged from 88 to 203 mmHg (mean ± sd 143 ± 33
mmHg) and 7/20 (35%) dogs were found to be hypertensive. In HC dogs, BP ranged
from 116 to 200 mmHg (mean ± sd 167 ± 26 mmHg) and 9/14 (64%) dogs were
hypertensive. Blood pressure and proteinuria were not significantly correlated.
Furthermore, in the DM group, U:P/C and U:A/C were both increased in 3
hypertensive dogs and 2 normotensive dogs while the only increase of U:A/C was
observed in 2 hypertensive and 3 normotensive dogs. In the HC group, the U:P/C and
the U:A/C were both increased in 6 hypertensive and 2 normotensive dogs; the U:A/C
was the sole increased parameter in 1 hypertensive dog and in 1 dog with normal
pressure.
DISCUSSION AND CONCLUSION- The findings of this study suggest that, in
dogs affected by DM and HC, an increase in U:P/C, U:A/C and systemic hypertension
is frequently present. Remarkably, some dogs affected by both DM and HC showed
an U:A/C but not U:P/C above the reference range. In diabetic dogs, albuminuria was
observed in 25% of the subjects, suggesting the possibility that this parameter could
be employed for detecting renal damage at an early phase when common
semiquantiative tests and even U:P/C fall inside the reference range. In HC dogs, a
higher number of subjects with overt proteinuria was found while only 14% presented
an increase only in the U:A/C. This fact, associated with a greater number of
hypertensive dogs having HC rather than DM, could suggest a greater influence on
renal function by the mechanisms involved in hypertension secondary to
hypercortisolemia. Furthermore, it is possible that, in HC dogs, the diagnosis was
more delayed than in DM dogs. However, the lack of a statistically significant
correlation between hypertension and increased protein excretion as well as the
apparently random distribution of proteinuric subjects in normotensive and
hypertensive cases, imply that other factors besides hypertension are involved in
causing proteinuria. Longitudinal studies are needed to further investigate the
relationship between hypertension and proteinuria.
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Prevalenza dell'ipotiroidismo nel cane affetto da spondilomielopatia cervicale caudaleGernone, Floriana <1974> 25 May 2009 (has links)
No description available.
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Studio sull'Eziopatogenesi dei Tumori Corticosurrenalici Cortisolo Secernenti nel CaneShehdula, Dardan <1978> 10 May 2010 (has links)
No description available.
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