Made available in DSpace on 2014-06-12T15:51:44Z (GMT). No. of bitstreams: 2 arquivo4521_1.pdf: 3119722 bytes, checksum: 150947b593a878f27c87a2a02052057f (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005 / A interferência da bactéria P. aeruginosa sobre o grupo coliforme é conhecida nas análises de água e sua enumeração tem sido questionada durante os últimos anos por ser considerada apenas um patógeno oportunista não habitante do trato intestinal de animais de sangue quente. No entanto, a grande versatilidade metabólica, daria vantagens à bactéria P. aeruginosa sobre outros microrganismos presentes na água. Neste trabalho, foi investigada a ação antagonista de 16 linhagens da bactéria P.aeruginosa, isoladas de diferentes amostras de águas de consumo humano e águas residuárias, contra bactérias do grupo coliforme. A existência de antagonismo foi observada em todas as linhagens no teste em meio sólido. As três linhagens mais agressivas foram submetidas ao teste em meio líquido. O contato se deu entre a bactéria P. aeruginosa com 72h de vantagem de crescimento, contra bactérias do grupo coliforme com inóculo inicial cerca de 102UFC/mL em caldo Műeller-Hinton em uma concentração de 10% da composição original. Entre 24h e 96h após o contato, o NMP/100mL das duas populações foi estimado utilizando a técnica dos tubos múltiplos em série de cinco. O fenômeno de antagonismo foi observado quando os inóculos iniciais partiram de 102UFC/mL e evidenciado quando a piocianina estava presente. E. aerogenes se mostrou mais sensível que E. coli. Este trabalho reascende uma discussão sobre a inclusão da bactéria P. aeruginosa como uma ferramenta adicional nas análises de água de consumo

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Submitted by (edna.saturno@ufrpe.br) on 2016-10-19T14:10:20Z No. of bitstreams: 1 Marluce de Souza.pdf: 916762 bytes, checksum: 7d8221ead71185e325b1a223a33b851c (MD5) / Made available in DSpace on 2016-10-19T14:10:20Z (GMT). No. of bitstreams: 1 Marluce de Souza.pdf: 916762 bytes, checksum: 7d8221ead71185e325b1a223a33b851c (MD5) Previous issue date: 2012-08-13 / Food is one of the key elements of tourist motivation and its quality is directly related to drinking water. This study aimed to assess the quality of the water used in food service hotels of the 6th Regional Municipality of Jaboatão Guararapes, PE, Brazil. 15 hotels were inspected and collected 31 water samples for physico-chemical and microbiological between June to December 2009. The hotels were grouped into large (HG) and medium (HM). To determine the Most Probable Number (MPN) of total coliform (TC), thermotolerant (Ct) Broth Lauryl Sulfate Tryptose (CLST) and Pseudomonas aeruginosa in Asparagine Broth used the technique of multiple tubes. A reassessment of the identification and confirmation of fecal coliform samples was performed by PCR. For quantification and identification of Aeromonas samples were used to direct plating method on starch agar with 20 ug / ml ampicillin and add starch 10g/1L for counting pattern (CFU / mL). All health establishments showed irregularity. The turbidity, sulfate and nitrite showed normal values, while chloride, pH and residual chlorine were out of specification. Water wells were contaminated by 25.0% (HG) for CT and Ct and 44.4% and 55.5% (HM) respectively. In reservoirs, the percentage of contamination hotels showed 75.0% (HG) and 77.7% (HM) for CT and Ct. It was observed that 66.6% of the reservoirs showed no contamination at the main entrance of the well water and public water supply. Of the 104 isolates of coliforms analyzed, 15% were species pathogenic environmental and 53.85% have been identified Buttiauxella harsh, Enterobacter aerogenes, Enterobacter gergoviae, Klebsiella ozaenae, Klebsiella planticola, terrigenous Klebsiella, Serratia liquefaciens and Serratia odor, Citrobacter amalonaticus, Citrobacter sp, Enterobacter sp, Enterobacter sakazakii, Escherichia coli, Klebsiella pneumoniae, Kluyvera ascorbata, myxofaciens Proteus and Providencia sp. The 41 isolates of Escherichia coli have been identified: Stx (-), eae (+) 3 (7.31%); Stx (-), eae (-) 21 (51.21%); eae (+) 1 ( 2.43%); Stx (+), eae (+) 1 (2.43%); StxM1M2 (-), eae (-) 10 (24.3%) PAA (-) aggR (-) 4 (9.75%) PAA (-) aggR (-) multicoli (+), eae (+) EPEC 1 (2.43%). Six (14.63%) strains of E. coli showed virulence factor, with 16.6% originating from reservoirs (HG) and 83.33% (HM). In the pit, Pseudomonas aeruginosa was detected in 25.0% (HG) and 75.0% (HM) and the reservoirs contamination was 75.0% (HG) and 77.7% (HM). , Aeromonas spp were isolated from all water samples (HG) with contamination ranging from 9.0 x 10, 1.9 x 105 wells and 2.0 x 10, 1.4 x 103 CFU / mL in the reservoirs. As for (HM) variation was 2.6 x10, 2.0 x104 CFU / mL in the well and 4.0 x101, 25 x 105 CFU / mL in the reservoir. Of (HM) that used the public water supply values were obtained from 4.0 x 103, 7.0 x 102 CFU / mL. Of the 48 isolates of Aeromonas species were 81.2% and 18.8% environmental pathogenic species have been identified A. schubertii, A. encheleia, A. eucrenophila, A. sober, A. veronii veronii, A. caviae, A. jandaei . The water of the hotel chain of Jaboatão Guararapes -6 th Region showed unsatisfactory levels of bacteriological quality of drinking water, although the water source, coming from public water supply be of good quality and free from contamination as the total and fecal coliforms. The lack of sanitation, rationing and intermittent supply of treated water, requiring the use of water reservoirs which favors the increased likelihood of water contamination. / O alimento é um dos principais elementos de motivação turística e a sua qualidade está diretamente relacionada com a potabilidade da água. Objetivou-se avaliar a qualidade da água utilizada em serviço de alimentação de hotéis da 6ª regional do município do Jaboatão dos Guararapes, PE, Brasil. Foram inspecionados 15 hotéis e coletadas 31 amostras de água para análises físico-química e microbiológica, entre junho a dezembro 2009. Os hotéis foram agrupados em grande porte (HG) e médio porte (HM). Para determinação do Número Mais Provável (NMP) de coliformes totais (CT), termotolerantes (Ct) em Caldo Lauril Sulfato Triptose (CLST) e Pseudomona aeruginosa em Caldo Asparagina utilizou-se a técnica dos tubos múltiplos. A confirmação da identificação e reavaliação das amostras de coliformes termotolerantes foi realizada pela técnica da PCR. Para a quantificação e identificação de Aeromonas as amostras utilizaram-se o método de plaqueamento direto em Ágar Amido com 20 μg/mL de ampicilina e suplemento de amido 10g/1L para a contagem padrão (UFC/mL). Todos os estabelecimentos apresentaram irregularidade sanitária. A turbidez, sulfato e nitrito apresentaram valores normais, enquanto cloreto, pH e o cloro residual estavam fora das especificações. Águas de poços apresentaram contaminação de 25,0% (HG) para CT e Ct e de 44,4% e de 55,5% (HM) respectivamente. Nos reservatórios, os hotéis apresentaram percentual de contaminação 75,0% (HG) e 77,7% (HM) para CT e Ct. Observou-se que 66,6% dos reservatórios não apresentaram contaminação na entrada principal do poço e da água abastecimento público. Dos 104 isolados de coliformes analisados, 15% foram espécies ambientais e 53,85% patogênicas, tendo sido identificadas Buttiauxella agreste, Enterobacter aerogenes, Enterobacter gergoviae, Klebsiella ozaenae, Klebsiella planticola, Klebsiella terrigena, Serratia liquefaciens e Serratia odorífera, Citrobacter amalonaticus, Citrobacter sp, Enterobacter sp, Enterobacter sakazakii, Escherichia coli, Klebsiella pneumoniae, Kluyvera ascorbata, Proteus myxofaciens e Providencia sp. Os 41 isolados de Escherichia coli, foram identificados: Stx (-), eae(+) 3(7,31%); Stx(-),eae(-) 21(51,21%); eae(+) 1(2,43%); Stx(+),eae(+) 1(2,43%); StxM1M2(-),eae(-) 10(24,3%); pAA(-) ,aggR(-) 4(9,75%); pAA(-) ,aggR(-) multicoli(+), eae(+), EPEC 1(2,43%). Seis (14,63%) cepas de E. coli apresentaram fator de virulência, sendo 16,6% originárias de reservatórios (HG) e 83,33% (HM). No poço, Pseudomonas aeruginosa foi detectada em 25,0% (HG) e 75,0% (HM) e nos reservatórios a contaminação ocorreu em 75,0% (HG) e em 77,7% (HM). As Aeromonas spp foram isoladas de todas as amostras de água dos (HG) com contaminação variando de 9,0 x 10, 1,9 x 105 nos poços e 2,0 x 10, 1,4 x 103 UFC/mL nos reservatórios. Quanto aos (HM) a variação foi de 2,6x10, 2,0x104 UFC/mL no poço e 4,0x101, 25x105 UFC/mL nas dos reservatórios. Dos (HM) que utilizavam água de abastecimento público obtiveram-se valores entre 4.0 x 103, 7.0 x 102 UFC/mL. Dos 48 isolados de Aeromonas, 81.2% eram espécies ambientais e 18,8% espécies patogênicas, tendo sido identificadas A. schubertii, A. encheleia, A. eucrenophila, A. sóbria, A. veronii veronii, A. caviae, A. jandaei.. A água da rede hoteleira do município de Jaboatão dos Guararapes -6ª Região apresentou níveis insatisfatórios de qualidade bacteriológica de água para consumo humano, apesar da água da origem, procedente de abastecimento público ser de boa qualidade e isenta de contaminação quanto aos coliformes totais e termotolerantes. A falta de saneamento básico, o racionamento e a intermitência do fornecimento de água tratada, exige a utilização de reservatórios de água o que favorece o aumento da probabilidade de contaminação da água.

Submitted by (edna.saturno@ufrpe.br) on 2016-07-28T13:32:52Z No. of bitstreams: 1 Andrea Paiva Botelho Lapenda Moura.pdf: 326144 bytes, checksum: 620eeb1d70babd2fa43af8e1560d7095 (MD5) / Made available in DSpace on 2016-07-28T13:32:52Z (GMT). No. of bitstreams: 1 Andrea Paiva Botelho Lapenda Moura.pdf: 326144 bytes, checksum: 620eeb1d70babd2fa43af8e1560d7095 (MD5) Previous issue date: 2006-02-20 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Evaluation of microbiological quality of chilled and in natural goat meat sold in public/private markets and supermarkets of Recife concerning Staphylococcus aureus counting, total and thermotolerant coliforms,identification and typing of Salmonella spp, was the objective of this study. Antimicrobial sensibility profile in vitro of Staphylococcus aureus and Salmonella spp samples and the correlation between the simultaneous presence of faecal coliforms and Salmonella spp. were also performed. The study was carried out at six public/private markets and nine supermarkets of Recife, which were selected according to the administrative division of Local Authority Healthy General Office in six sanitary districts. A total of 24 samples of goat meat, 14 proceeded from public/private markets in natura and 10 chilled ones from supermarkets. Samples were processed using methodologies recommended by the Agriculture, Pecuary and Provision Ministry. Results demonstrated that 11 samples (45,83%) presented positive coagulasis staphylococcus (SCP) varying from 6.7 x 103 to 1.4 x 106 UFC/g, corresponding to 27 isolated from a total of 89. In total coliforms analyses, five samples (20.83%) presented satisfactory sanitary conditions and 19 (79.16%) had countings varying from 4.3 x 103 to 3.7 x 107, thermotolerants coliforms were detected in 15 (62.5%), which countings varied from 2 x 104 to 2.5 x 107 UFC/g and nine samples (37.5%) presented satisfactory sanitary conditions. Presence of Salmonella spp., was confirmed at seven samples (29.17%) in which eight sorovares were typed: four (50%) Salmonella enterica subsp houtenae (O:53), two (25%) S. Anatum, one (12.50%) S.Rubislaw and one (12, 50%) S. Derby. No correlation was observed among coliforms and Salmonella presure. Antimicrobial profile sensibility showed that S. aureus were sensitive to norfloxacin and vancomicin (100%), tetraciclin and sulpha + trimetoprim (96.30%) and oxacilin (87.50%) and 100 % of Salmonella spp. samples were sensitive norfloxacin, 75% sulpha + trimetoprim and 62.5% tetraciclin. It is concluded that goat meat sold at markets and supermarkets of Recife presented undesirable microbiological patterns emphasizing the importance of a strong supervision at the productive goat meat chain as well as a revision of the Resolution of Collective Management from the National Agency of Sanitary Vigilance, (RDC, n°12/2001) which does not establish microbiological patterns for the coliforms and Staphylococcus spp countings in chilled and in natura goat meat. / Objetivou-se com este estudo avaliar a qualidade microbiológica da carne caprina in natura e resfriada, comercializada em mercados públicos/privados e supermercados na Cidade do Recife, quanto a contagem de Staphylococcus aureus, coliformes totais e termotolerantes, e identificação e tipificação de Salmonella spp. Realizou-se, também, o perfil de sensibilidade antimicrobiana in vitro de amostras de Staphylococcus aureus e Salmonella spp e a correlação entre a presença simultânea de coliformes fecais e Salmonella spp. O estudo foi realizado em seis mercados públicos/privados e nove supermercados da Cidade do Recife, selecionados seguindo a divisão administrativa da Secretaria de Saúde do Município em seis Distritos Sanitários. Foram analisadas 24 amostras de carne caprina, sendo 14 in natura procedentes de mercados públicos/privados e 10 resfriadas de supermercados. As amostras foram processadas utilizando metodologias preconizadas pelo Ministério da Agricultura, Pecuária e Abastecimento. Os resultados demonstraram que 11 (45,83%) amostras apresentaram contagens de estafilococos coagulase positiva (SCP) variando de 6,7 x 103 a 1,4 x 106 UFC/g, correspondendo a 27 isolados de um total de 89. Para coliformes totais, cinco (20,83%) amostras encontravam-se em condições sanitárias satisfatórias e 19 (79,16%) apresentaram contagens variando de 4,3 x 103 a 3,7 x 107; e para termotolerantes 15 (62,5%) apresentaram contagens variando de 1,2 x 104 a 2,5 x 107 UFC/g e nove (37,5%) encontravam-se em condições sanitárias satisfatórias. Foram confirmadas em sete (29,17%) amostras a presença de Salmonella spp., nas quais foram tipificadas oito sorovares: quatro (50,00%) Salmonella enterica subsp houtenae (O:53), duas (25,00%) S. Anatum, uma (12,50%) S.Rubislaw, uma (12,50%) S. Derby. Não se observou correlação entre as bactérias estudadas. O perfil de sensibilidade antimicrobiana demonstrou que os antibióticos mais indicados para o tratamento de S. aureus foram norfloxacina e vancomicina (100%), tetraciclina e sulfa + trimetoprim (96,30%) e oxacilina (87,50%) e para Salmonella spp. 100% das amostras foram sensíveis à norfloxacina, 75,00% à sulfa + trimetoprim e 62,5% para tetraciclina. Conclui-se que a carne caprina comercializada nos mercados e supermercados da Cidade do Recife apresentou padrões microbiológicos indesejáveis ressaltando a importância da necessidade de uma maior fiscalização na cadeia produtiva da carne caprina bem como rever a Resolução da Diretoria Colegiada da Agência Nacional de Vigilância Sanitária (RDC, n°12/2001) que não estabelece padrões microbiológicas para a contagem de coliformes termotolerantes e Staphylococcus spp. em carne caprina in natura e resfriada.

Die Uferfiltration (UF) ist eine potentielle Alternative zur konventionellen Oberflächenwasseraufbereitung in Indien, da Trübstoffe, pathogene Mikroorganismen und organische Wasserinhaltsstoffe effektiv entfernt werden. In dieser Arbeit wurde erstmals ein umfangreicher Überblick zu bestehenden UF-Anlagen in Indien erarbeitet. Für die Standorterkundung und -bewertung wurde ein Konzept erarbeitet, das an drei Standorten entlang des Ganges getestet und weiterentwickelt wurde. Das Konzept umfasst vier Stufen: Standortvorerkundung, Bestimmung von Grundwasserleiterparametern, Erfassung von hydraulischen und Beschaffenheits-parametern sowie numerische Grundwasser-strömungsmodellierung. Entlang des oberen Flusslaufes des Ganges (Haridwar und Srinagar) wurden günstige geohydraulische Verhältnisse identifiziert (kf = 10E-4 bis 10E-3 m/s, Grundwasser leitermächtigkeit 11 bis 20 m). Entlang des unteren Flusslaufes (Patna) gibt es in Abhängigkeit von der Mächtigkeit der Sedimentablagerungen im Ganges nur bei erhöhter Schleppkraft im Monsun eine gute hydraulische Verbindung zwischen dem Fluss und dem Grundwasserleiter. In Haridwar wurde der Uferfiltratanteil im Rohwasser mittels Isotopenanalysen (δ18O) und Leitfähigkeitsmessungen im Fluss- und Rohwasser ermittelt. Der Uferfiltratanteil in den auf einer Insel und südlich davon gelegenen Brunnen liegt bei bis zu 90%. An den untersuchten Standorten wird durch die UF eine effektive Entfernung von E. coli um 3,5 bis 4,4 Log10 und der Trübung bis >2 Log10 Einheiten erreicht. Eine Entfernung von 3 Log10 Einheiten wurde bereits bei einer Fließzeit des Uferfiltrats von zwei Tagen beobachtet. Die erhöhte Anzahl an Coliformen in einigen Brunnen am Standort Haridwar resultiert aus Verunreinigungen des landseitigen Grundwassers. Bei Hochwässern und Starkregenereignissen muss eine Kontamination durch den direkten Eintrag von Wasser durch undichte Brunnenabdeckungen, Risse in den Schächten bzw. unsachgemäßen Brunnenbau berücksichtigt werden. Die Anwendung des angepassten Untersuchungskonzepts an 15 weiteren UF-Standorten in Indien hat gezeigt, dass die niedrigen DOC-Konzentrationen im Flusswasser (0,9 bis 3,0 mg/L) und im Brunnenwasser (0,4 bis 2,3 mg/L) günstig für die Anwendung der UF sind. Bei erhöhten DOC-Konzentrationen (Vormonsun) im Flusswasser konnte in Delhi und Mathura im Monsun eine 50%ige Verminderung erreicht werden. Bei der Erkundung neuer UF-Standorte in bergigen Gebieten sind die Grundwasserleitermächtigkeit mit geophysikalischen Erkundungsverfahren, die Strömungsverhältnisse in den alluvialen Ablagerungen sowie lokale Hochwasserrisiken zu untersuchen. / Riverbank filtration or bank filtration (RBF / BF) is a potential alternative to the direct abstraction and conventional treatment of surface water by virtue of the effective removal of pathogens, turbidity, suspended particles and organic substances. A comprehensive overview of existing RBF systems in India has been compiled for the first time. To systematically select and investigate new and existing potential RBF sites in India, a methodological concept was developed and tested at three sites along the Ganga River. The four stages of the concept are: initial site-assessment, basic site-survey, monitoring of water quality and quantity parameters and determination of aquifer parameters and numerical groundwater flow modelling. Suitable geohydraulic conditions for RBF (hydraulic conductivity: 10E-4 to 10E-3 m/s, aquifer thickness: 11 to 20 m) exist along the upper course of the Ganga (Haridwar and Srinagar). Due to the presence of fine sediment layers beneath the river bed along the Ganga’s lower course (Patna), river-aquifer interaction occurs during increased shear stress on the riverbed in monsoon. The portion of bank filtrate abstracted by the wells in Haridwar was determined from isotope analyses (Oxygen 18) and electrical conductivity measurements of river and well water and is up to 90% for wells located on an island and between the river and a canal. The results were confirmed by groundwater flow modelling. A high removal of E. coli (3.5 to 4.4 Log10 units) and turbidity (>2 Log10 units) was observed at the investigated sites. An E. coli removal of 3 Log10 units was observed for short travel times of 2 days. Higher coliform counts in some wells occur due to contamination from landside groundwater. During floods and intense rainfall events, contamination of RBF wells from direct entry of flood water, seepage of surface runoff into the well through leaky covers, fissures in the well-heads / caissons and in-appropriately sealed well-bases has to be considered. The application of the adapted investigation concept to 15 other sites in India showed that the low DOC concentrations in river water (0.9 to 3.0 mg/L) and well-water (0.4 to 2.3 mg/L) are favourable for the application of RBF. A 50% decrease of the high (pre-monsoon) DOC concentration was observed during monsoon in Delhi and Mathura. For the exploration of new RBF sites in hilly / mountainous areas, investigations of the aquifer thickness using geophysical methods, subsurface flow conditions in the alluvial deposits and the risk from floods should be conducted.

Die Uferfiltration (UF) ist eine potentielle Alternative zur konventionellen Oberflächenwasseraufbereitung in Indien, da Trübstoffe, pathogene Mikroorganismen und organische Wasserinhaltsstoffe effektiv entfernt werden. In dieser Arbeit wurde erstmals ein umfangreicher Überblick zu bestehenden UF-Anlagen in Indien erarbeitet. Für die Standorterkundung und -bewertung wurde ein Konzept erarbeitet, das an drei Standorten entlang des Ganges getestet und weiterentwickelt wurde. Das Konzept umfasst vier Stufen: Standortvorerkundung, Bestimmung von Grundwasserleiterparametern, Erfassung von hydraulischen und Beschaffenheits-parametern sowie numerische Grundwasser-strömungsmodellierung. Entlang des oberen Flusslaufes des Ganges (Haridwar und Srinagar) wurden günstige geohydraulische Verhältnisse identifiziert (kf = 10E-4 bis 10E-3 m/s, Grundwasser leitermächtigkeit 11 bis 20 m). Entlang des unteren Flusslaufes (Patna) gibt es in Abhängigkeit von der Mächtigkeit der Sedimentablagerungen im Ganges nur bei erhöhter Schleppkraft im Monsun eine gute hydraulische Verbindung zwischen dem Fluss und dem Grundwasserleiter. In Haridwar wurde der Uferfiltratanteil im Rohwasser mittels Isotopenanalysen (δ18O) und Leitfähigkeitsmessungen im Fluss- und Rohwasser ermittelt. Der Uferfiltratanteil in den auf einer Insel und südlich davon gelegenen Brunnen liegt bei bis zu 90%. An den untersuchten Standorten wird durch die UF eine effektive Entfernung von E. coli um 3,5 bis 4,4 Log10 und der Trübung bis >2 Log10 Einheiten erreicht. Eine Entfernung von 3 Log10 Einheiten wurde bereits bei einer Fließzeit des Uferfiltrats von zwei Tagen beobachtet. Die erhöhte Anzahl an Coliformen in einigen Brunnen am Standort Haridwar resultiert aus Verunreinigungen des landseitigen Grundwassers. Bei Hochwässern und Starkregenereignissen muss eine Kontamination durch den direkten Eintrag von Wasser durch undichte Brunnenabdeckungen, Risse in den Schächten bzw. unsachgemäßen Brunnenbau berücksichtigt werden. Die Anwendung des angepassten Untersuchungskonzepts an 15 weiteren UF-Standorten in Indien hat gezeigt, dass die niedrigen DOC-Konzentrationen im Flusswasser (0,9 bis 3,0 mg/L) und im Brunnenwasser (0,4 bis 2,3 mg/L) günstig für die Anwendung der UF sind. Bei erhöhten DOC-Konzentrationen (Vormonsun) im Flusswasser konnte in Delhi und Mathura im Monsun eine 50%ige Verminderung erreicht werden. Bei der Erkundung neuer UF-Standorte in bergigen Gebieten sind die Grundwasserleitermächtigkeit mit geophysikalischen Erkundungsverfahren, die Strömungsverhältnisse in den alluvialen Ablagerungen sowie lokale Hochwasserrisiken zu untersuchen.:Abstract i (Seitenzahl / page number) Acknowledgements iii Table of contents v List of tables viii List of figures ix Abbreviations and symbols xi 1 Introduction 1 1.1 Problem description 1 1.2 Riverbank filtration and its potential in India 2 1.3 Motivation 3 1.4 Aims 4 2 Bank filtration in context to India’s water resources 5 2.1 Water budget of India and the Ganga River catchment 5 2.1.1 Water budget 5 2.1.2 The Ganga River catchment 6 2.2 Problems of surface water abstraction for drinking water production 8 2.2.1 Effect of low surface flows on the quantity of raw water abstraction 8 2.2.2 Effect of the monsoon on conventional drinking water treatment plants using directly abstracted surface water 9 2.2.3 Quality of surface water 10 2.2.4 Treatment of directly abstracted surface water for drinking 11 2.3 Sustainability issues of groundwater abstraction 11 2.4 Drinking water consumption in India 12 2.5 Bank filtration for water supply 14 2.5.1 Geohydraulic, siting and design aspects of bank filtration systems 14 2.5.2 Water quality aspects 15 2.5.3 Water quality aspects for bank filtration in India 15 2.5.4 Risks to riverbank filtration sites from floods 16 2.6 Hypotheses favouring the use of bank filtration and the need for a concept to investigate potential RBF sites in India 17 3 Study areas 18 3.1 Choice of study areas 18 3.2 Case study site Haridwar 19 3.3 Case study site Patna 20 3.4 Case study site Srinagar in Uttarakhand 21 3.5 Hypotheses favouring RBF at the selected study sites 22 4 Methodology for the investigation of the case study sites 24 4.1 Overview of methodology for investigating the case-study sites 24 4.2 Investigations at the case study site of Haridwar 25 4.2.1 Initial site-assessment 25 4.2.2 Basic site-survey and establishing monitoring infrastructure 26 4.2.2.1 Identification of specific locations for monitoring wells 26 4.2.2.2 Geodetic survey and inventory of existing on-site infrastructure 26 4.2.2.3 Construction of exploratory wells 27 4.2.3 Determination of hydrogeological parameters 27 4.2.3.1 Sediment analyses 27 4.2.3.2 Determination of hydraulic conductivity by pump tests on large-diameter wells 29 4.2.4 Water level and stable isotope measurements 30 4.2.4.1 Water level 30 4.2.4.2 Stable isotopes 31 4.2.5 Water quality monitoring 31 4.2.5.1 Initial investigations, screening and formulation of monitoring concept 31 4.2.5.2 Comprehensive and regular monitoring 2011 - 2013 33 4.3 Investigations at the case study site of Patna 34 4.3.1 Initial site-assessment, basic-site survey and monitoring 34 4.3.2 Sampling for water quality and isotope analyses 35 4.4 Investigations at the case study site of Srinagar in Uttarakhand 35 4.4.1 Basic site-survey and establishing monitoring infrastructure 35 4.4.1.1 Identification of a specific location for a new RBF well 35 4.4.1.2 Construction of production and monitoring wells and exploratory boreholes 37 4.4.2 Determination of hydrogeological parameters and monitoring 38 4.4.2.1 Sediment analyses and determination of hydraulic conductivity of the aquifer 38 4.4.3 Water quality monitoring 40 4.5 Column experiments to determine the removal of bacteriological indicators under field conditions 40 5 Characterisation of the RBF system in Haridwar 42 5.1 Site and design aspects 42 5.1.1 Location of RBF wells 42 5.1.2 Design of RBF wells 44 5.1.3 Quantity of drinking water produced by RBF 45 5.2 Aquifer characterisation 47 5.3 Numerical groundwater flow model of RBF well field in Haridwar 49 5.3.1 Model set-up 49 5.3.2 Model calibration 50 5.4 Origin of water and mean portion of bank filtrate abstracted by RBF wells 52 5.5 Water quality 53 5.6 Analysis of presence of thermotolerant coliforms in RBF wells 56 5.7 Impact of regulated Upper Ganga Canal on RBF wells on Pant Dweep 58 5.8 Summary of case study site Haridwar 60 5.8.1 Aspects related to water quality 60 5.8.2 Benefit of groundwater flow modelling 60 6 Evaluation of the potential for RBF in Patna 62 6.1 Physiography and hydrogeology 62 6.1.1 South Ganga Plain 62 6.1.2 Patna 63 6.2 Ground and surface water levels 65 6.3 Ganga River morphology 66 6.4 Water quality 67 6.5 Numerical groundwater flow model of case study site Patna 68 6.5.1 Model geometry and initial conditions 68 6.5.2 Boundary conditions 69 6.5.3 Steady-state flow modelling 70 6.6 Isotope analyses 71 6.7 Summary of case study site Patna 71 7 Evaluation of the potential for RBF in Srinagar 73 7.1 Drinking water production and overview of geomorphology 73 7.2 RBF site characterisation 74 7.2.1 Aquifer geometry and material 74 7.2.2 Water levels 75 7.2.3 Hydraulic conductivity 76 7.3 Numerical groundwater flow model of case study site Srinagar 77 7.3.1 Model geometry and calibration 77 7.3.2 Origin of bank filtrate and travel time 78 7.4 Water quality 79 7.5 Discussion and summary of case study site Srinagar 81 8 Assessment of risks from floods and insufficient sanitary measures to RBF wells in Haridwar and Srinagar 82 8.1 Flood-risk identification from field investigations 82 8.1.1 Description of an extreme flood event in Haridwar 82 8.1.2 Description of an extreme flood event in Srinagar 82 8.1.3 Summary of identifiable risks 83 8.2 Assessment of risks to RBF wells 84 8.2.1 Design of wells and direct contamination 84 8.2.2 Field investigations on the removal of bacteriological indicators 85 8.2.3 Removal of coliforms under field conditions by column experiments 87 8.3 Proposals to mitigate risks at RBF sites Haridwar and Srinagar 89 8.3.1 Operational and technical aspects for a general risk management plan 89 8.3.2 Health aspects for a general risk management plan 89 8.3.3 Criteria for flood protection measures of RBF wells 90 8.3.4 Sanitary sealing of RBF wells 90 9 Application of initial site-assessment to investigate other RBF sites in India 92 9.1 Hydrogeology and system-design 92 9.1.1 RBF systems for small and large-scale urban water supply 92 9.1.2 “Koop” well RBF systems for small-scale rural water supply 98 9.2 Water quality parameters 98 9.2.1 Removal of bacteriological indicators by RBF 98 9.2.2 Removal of dissolved organic carbon and organic micropollutants by RBF 101 9.2.3 Inorganic parameters 102 10 Conclusions, recommendations and propagation of RBF 105 10.1 Hydrogeological and system-design considerations 105 10.2 Aspects for improvement of the concept for RBF site investigations 106 10.3 Policy and planning aspects for the propagation of RBF in India 108 References 110 Annexes 121 / Riverbank filtration or bank filtration (RBF / BF) is a potential alternative to the direct abstraction and conventional treatment of surface water by virtue of the effective removal of pathogens, turbidity, suspended particles and organic substances. A comprehensive overview of existing RBF systems in India has been compiled for the first time. To systematically select and investigate new and existing potential RBF sites in India, a methodological concept was developed and tested at three sites along the Ganga River. The four stages of the concept are: initial site-assessment, basic site-survey, monitoring of water quality and quantity parameters and determination of aquifer parameters and numerical groundwater flow modelling. Suitable geohydraulic conditions for RBF (hydraulic conductivity: 10E-4 to 10E-3 m/s, aquifer thickness: 11 to 20 m) exist along the upper course of the Ganga (Haridwar and Srinagar). Due to the presence of fine sediment layers beneath the river bed along the Ganga’s lower course (Patna), river-aquifer interaction occurs during increased shear stress on the riverbed in monsoon. The portion of bank filtrate abstracted by the wells in Haridwar was determined from isotope analyses (Oxygen 18) and electrical conductivity measurements of river and well water and is up to 90% for wells located on an island and between the river and a canal. The results were confirmed by groundwater flow modelling. A high removal of E. coli (3.5 to 4.4 Log10 units) and turbidity (>2 Log10 units) was observed at the investigated sites. An E. coli removal of 3 Log10 units was observed for short travel times of 2 days. Higher coliform counts in some wells occur due to contamination from landside groundwater. During floods and intense rainfall events, contamination of RBF wells from direct entry of flood water, seepage of surface runoff into the well through leaky covers, fissures in the well-heads / caissons and in-appropriately sealed well-bases has to be considered. The application of the adapted investigation concept to 15 other sites in India showed that the low DOC concentrations in river water (0.9 to 3.0 mg/L) and well-water (0.4 to 2.3 mg/L) are favourable for the application of RBF. A 50% decrease of the high (pre-monsoon) DOC concentration was observed during monsoon in Delhi and Mathura. For the exploration of new RBF sites in hilly / mountainous areas, investigations of the aquifer thickness using geophysical methods, subsurface flow conditions in the alluvial deposits and the risk from floods should be conducted.:Abstract i (Seitenzahl / page number) Acknowledgements iii Table of contents v List of tables viii List of figures ix Abbreviations and symbols xi 1 Introduction 1 1.1 Problem description 1 1.2 Riverbank filtration and its potential in India 2 1.3 Motivation 3 1.4 Aims 4 2 Bank filtration in context to India’s water resources 5 2.1 Water budget of India and the Ganga River catchment 5 2.1.1 Water budget 5 2.1.2 The Ganga River catchment 6 2.2 Problems of surface water abstraction for drinking water production 8 2.2.1 Effect of low surface flows on the quantity of raw water abstraction 8 2.2.2 Effect of the monsoon on conventional drinking water treatment plants using directly abstracted surface water 9 2.2.3 Quality of surface water 10 2.2.4 Treatment of directly abstracted surface water for drinking 11 2.3 Sustainability issues of groundwater abstraction 11 2.4 Drinking water consumption in India 12 2.5 Bank filtration for water supply 14 2.5.1 Geohydraulic, siting and design aspects of bank filtration systems 14 2.5.2 Water quality aspects 15 2.5.3 Water quality aspects for bank filtration in India 15 2.5.4 Risks to riverbank filtration sites from floods 16 2.6 Hypotheses favouring the use of bank filtration and the need for a concept to investigate potential RBF sites in India 17 3 Study areas 18 3.1 Choice of study areas 18 3.2 Case study site Haridwar 19 3.3 Case study site Patna 20 3.4 Case study site Srinagar in Uttarakhand 21 3.5 Hypotheses favouring RBF at the selected study sites 22 4 Methodology for the investigation of the case study sites 24 4.1 Overview of methodology for investigating the case-study sites 24 4.2 Investigations at the case study site of Haridwar 25 4.2.1 Initial site-assessment 25 4.2.2 Basic site-survey and establishing monitoring infrastructure 26 4.2.2.1 Identification of specific locations for monitoring wells 26 4.2.2.2 Geodetic survey and inventory of existing on-site infrastructure 26 4.2.2.3 Construction of exploratory wells 27 4.2.3 Determination of hydrogeological parameters 27 4.2.3.1 Sediment analyses 27 4.2.3.2 Determination of hydraulic conductivity by pump tests on large-diameter wells 29 4.2.4 Water level and stable isotope measurements 30 4.2.4.1 Water level 30 4.2.4.2 Stable isotopes 31 4.2.5 Water quality monitoring 31 4.2.5.1 Initial investigations, screening and formulation of monitoring concept 31 4.2.5.2 Comprehensive and regular monitoring 2011 - 2013 33 4.3 Investigations at the case study site of Patna 34 4.3.1 Initial site-assessment, basic-site survey and monitoring 34 4.3.2 Sampling for water quality and isotope analyses 35 4.4 Investigations at the case study site of Srinagar in Uttarakhand 35 4.4.1 Basic site-survey and establishing monitoring infrastructure 35 4.4.1.1 Identification of a specific location for a new RBF well 35 4.4.1.2 Construction of production and monitoring wells and exploratory boreholes 37 4.4.2 Determination of hydrogeological parameters and monitoring 38 4.4.2.1 Sediment analyses and determination of hydraulic conductivity of the aquifer 38 4.4.3 Water quality monitoring 40 4.5 Column experiments to determine the removal of bacteriological indicators under field conditions 40 5 Characterisation of the RBF system in Haridwar 42 5.1 Site and design aspects 42 5.1.1 Location of RBF wells 42 5.1.2 Design of RBF wells 44 5.1.3 Quantity of drinking water produced by RBF 45 5.2 Aquifer characterisation 47 5.3 Numerical groundwater flow model of RBF well field in Haridwar 49 5.3.1 Model set-up 49 5.3.2 Model calibration 50 5.4 Origin of water and mean portion of bank filtrate abstracted by RBF wells 52 5.5 Water quality 53 5.6 Analysis of presence of thermotolerant coliforms in RBF wells 56 5.7 Impact of regulated Upper Ganga Canal on RBF wells on Pant Dweep 58 5.8 Summary of case study site Haridwar 60 5.8.1 Aspects related to water quality 60 5.8.2 Benefit of groundwater flow modelling 60 6 Evaluation of the potential for RBF in Patna 62 6.1 Physiography and hydrogeology 62 6.1.1 South Ganga Plain 62 6.1.2 Patna 63 6.2 Ground and surface water levels 65 6.3 Ganga River morphology 66 6.4 Water quality 67 6.5 Numerical groundwater flow model of case study site Patna 68 6.5.1 Model geometry and initial conditions 68 6.5.2 Boundary conditions 69 6.5.3 Steady-state flow modelling 70 6.6 Isotope analyses 71 6.7 Summary of case study site Patna 71 7 Evaluation of the potential for RBF in Srinagar 73 7.1 Drinking water production and overview of geomorphology 73 7.2 RBF site characterisation 74 7.2.1 Aquifer geometry and material 74 7.2.2 Water levels 75 7.2.3 Hydraulic conductivity 76 7.3 Numerical groundwater flow model of case study site Srinagar 77 7.3.1 Model geometry and calibration 77 7.3.2 Origin of bank filtrate and travel time 78 7.4 Water quality 79 7.5 Discussion and summary of case study site Srinagar 81 8 Assessment of risks from floods and insufficient sanitary measures to RBF wells in Haridwar and Srinagar 82 8.1 Flood-risk identification from field investigations 82 8.1.1 Description of an extreme flood event in Haridwar 82 8.1.2 Description of an extreme flood event in Srinagar 82 8.1.3 Summary of identifiable risks 83 8.2 Assessment of risks to RBF wells 84 8.2.1 Design of wells and direct contamination 84 8.2.2 Field investigations on the removal of bacteriological indicators 85 8.2.3 Removal of coliforms under field conditions by column experiments 87 8.3 Proposals to mitigate risks at RBF sites Haridwar and Srinagar 89 8.3.1 Operational and technical aspects for a general risk management plan 89 8.3.2 Health aspects for a general risk management plan 89 8.3.3 Criteria for flood protection measures of RBF wells 90 8.3.4 Sanitary sealing of RBF wells 90 9 Application of initial site-assessment to investigate other RBF sites in India 92 9.1 Hydrogeology and system-design 92 9.1.1 RBF systems for small and large-scale urban water supply 92 9.1.2 “Koop” well RBF systems for small-scale rural water supply 98 9.2 Water quality parameters 98 9.2.1 Removal of bacteriological indicators by RBF 98 9.2.2 Removal of dissolved organic carbon and organic micropollutants by RBF 101 9.2.3 Inorganic parameters 102 10 Conclusions, recommendations and propagation of RBF 105 10.1 Hydrogeological and system-design considerations 105 10.2 Aspects for improvement of the concept for RBF site investigations 106 10.3 Policy and planning aspects for the propagation of RBF in India 108 References 110 Annexes 121

The present study evaluates the surface water quality in the canals of An Giang province in the period from 2009 to 2016. The results showed that surface water of the canals was contaminated by organic matter and microorganisms which makes it not suitable for water supply and conservation of aquatic life. The water quality parameters such as dissolved oxygen (DO), biological oxygen demand (BOD), total suspended solids (TSS), orthophosphate (P-PO43-) and coliforms levels in the wet season were found to be higher than those in the dry season. The problem of organic and microorganic pollution over a long period of time without solutions leads to declines in water quality and then quantity as well. Agriculture is the main activity contributing to pollution of surface water in interior canals along with the activities of daily life, industry and services. This causes pollution of the surface water on Hau River due to its exchange of water with the connected canals. Good agricultural practices should be implemented to limit the pollution of surface water resources of the Mekong Delta. / Nghiên cứu này nhằm đánh giá diễn biến chất lượng nước mặt trong các kênh rạch nội đồng của tỉnh An Giang trong giai đoạn 2009 – 2016. Kết quả cho thấy nước mặt tại các kênh rạch nội đồng đã ô nhiễm hữu cơ và vi sinh vật. Nguồn nước không phù hợp cho mục đích cấp nước sinh hoạt và bảo tồn thực vật thủy sinh. Các chỉ tiêu như hàm lượng oxy hòa tan (DO), nhu cầu oxy sinh hóa (BOD), tổng chất rắn lơ lửng (TSS), orthophosphate (P-PO43-) và coliforms trong mùa mưa cao hơn mùa khô. Vấn đề ô nhiễm hữu cơ và vi sinh vật diễn ra trong thời gian dài và chưa có giải pháp xử lý làm cho chất lượng nước suy giảm dẫn đến suy giảm về trữ lượng. Nông nghiệp là hoạt động chính góp phần làm ô nhiễm nguồn nước mặt trong các kênh rạch nội đồng bên cạnh các hoạt động sinh hoạt, công nghiệp và dịch vụ. Điều này dẫn đến nước mặt trên sông Hậu cũng có đặt tính ô nhiễm tương tự do trao đổi nước với các kênh rạch nội đồng. Thực hành sản xuất nông nghiệp thân thiện môi trường cần sớm được triển khai để hạn chế ô nhiễm nguồn nước mặt quan trọng của khu vực đồng bằng sông Cửu Long.