This research aims to explore the links between cross-sector collaboration, a holistic multi-stakeholder approach, and Sustainable Development, and identify whether such a holistic approach can lead to better collaboration processes, and ultimately results. Specifically, it focuses on sustainability in relation to food, through the lense of a qualitative case-study on the city of Malmö, which aims to identify and implement a more sustainable food system through the development of a Food Partnership where diverse stakeholders from across society are invited to actively engage in the process on a relatively equal basis. The study explores these theoretical concepts through the research question: How can a cross-sector collaboration with a holistic multi-stakeholder approach be developed and sustained in the pursuit of the Sustainable Development Goals?The research uncovers the key factors which should be considered in order to form a holistic and long-term partnership, and based on these factors, an analytical framework is developed and used to assess the empirical findings and develop recommendations for the Malmö Food Partnership.This thesis provides a theoretical contribution by bridging the research gap between the concepts of cross-sector collaboration, a holistic multi-stakeholder approach and Sustainable Development. Furthermore, it also provides a practical contribution with its analytical framework model, which can be adapted to future partnerships for the Sustainable Development Goals in urban settings.