Abstract The purpose of this study was to investigate the inter-relation between sense of humor and conduct of innovation on a comparative analysis towards different personalities. The samples of this study were under the employment of 30 different public and private firms and organizations on questionnaires. The measure instruments used in this study include ¡§Multidimensional Sense of Humor Scale,¡¨ ¡§Conduct of Innovation,¡¨ ¡§Locus of Control Scales,¡¨ ¡§Type A Personality,¡¨ and ¡§Positive and Negative Affectivities Scales.¡¨ The effective samples of this study were 1213 employees. The survey was to prove that sense of humor is a predictable factor of the conduct of innovation. Also, it was to investigate if different personalities and other variables would do direct interferences to one¡¦s sense of humor, conduct of innovation and to every developing stage of the above mentioned. The variables of personality include ¡§Locus of Control,¡¨ ¡§Positive Affectivity¡¨ and ¡§Negative Affectivity.¡¨ The results of this study could be summarized as follows: 1.Sense of Humor and Conduct of Innovation are predictable. 2.¡§Locus of Control¡¨ is not an interference factor to one¡¦s sense of humor and conduct of innovation. 3.¡§Type A Personality¡¨ is an interference factor to one¡¦s sense of humor and conduct of innovation. 4.¡§Positive affectivity¡¨ is not an interference factor to one¡¦s sense of humor and conduct of innovation. 5.¡§Negative affectivity¡¨ is not an interference factor to one¡¦s sense of humor and conduct of innovation.