Submitted by Alberto Vieira (martins_vieira@ibest.com.br) on 2018-06-14T18:21:29Z No. of bitstreams: 1 278389.pdf: 17365317 bytes, checksum: 5f174436762a3168beb5c15b54229e85 (MD5) / Made available in DSpace on 2018-06-14T18:21:29Z (GMT). No. of bitstreams: 1 278389.pdf: 17365317 bytes, checksum: 5f174436762a3168beb5c15b54229e85 (MD5) Previous issue date: 1997 / CAPES / Corydoradinae, incluindo os gêneros Aspidoras, Brochis e Corydoras, constitui cerca de 90% da família de bagres neotropicais Callichthyidae, um grupo monofilético bem definido. Uma hipótese filogenética da subfamília é estabelecida com base em 58 caracteres, abrangendo aqueles propostos por REIS (1993) para definir Corydoradinae e suas unidades monofiléticas. Monofiletismo de Corydoradinae é confirmado, assim como de Aspidoras e Brochis. Dois dados principais compõem a subfamília, um com espécies de Corydoras mais relacionadas com Aspidoras do que com Brochis, e outro com espécies mais relacionadas com Brochis. Estes fatores contrastam com a hipótese prévia que considera Brochis e todas as espécies de Corydoras em um agrupamento monofilético, e Aspidoras como seu grupo-irmão. Um clado composto por Aspidoras, C. habrosus, C. paleatus, C. ehrhardti, C. nattereri, (C. grupo hastatus, C. grupo nanus, C. elegans, C. undulatus), (C. ellisae, C. ornatus, C. prionotus, C. reticulatus, C. stenocephalus, (C. septentrionalis (C. grupo barbatus, C. blochi, C. acutus)) é definido pela presença de odontódeos nos infra-orbitais e pré-opérculo, e pela margem livre do opérculo angulada. Um outro dado, ( C. flaveolus ( C. arcuatus ( C. grupo punctatus ( C. triseriatus ( C. garbei (C. aeneus (C. eques (C. grupo rabauti + Brochis)))))))) é definido pela perda de odontódeos no opérculo, homoplásico com Corydoras grupo hastatus. Algumas propostas de classificação são discutidas. / Phylogeny ofthe subfamily Corydoradinae (Siluriformes : Callichthyidae) Corydoradinae, comprising the genera Aspidoras, Brochis and Corydoras, constitutes about 90% of the neotropical catfish family Callichthyidae, a well-defined monophyletic group. A phylogenetic hypothesis within the subfamily is erected with basis on 58 characters, encompassing those proposed by REIS (1993) to define Corydoradinae and its monophyletic units. Monophyly of Corydoradinae is confirmed, as well as for Aspidoras and Brochis. Two major clades compose the subfamily, one with Corydoras species more closely related to Aspidoras than Brochis, and other with species closer to Brochis. These features contrast with the previous hypothesis considering Brochis and all Corydoras species in a monophyletic assemblage and Aspidoras as its sister-group. A clade composed of Aspidoras, C. habrosus, C. paleatus, C. ehrhardti, C. nattereri, (C. hastatus group, C. nanus group, C. elegans, C. undulatus), (C. ellisae, C. ornatus, C. prionotus, C. reticulatus, C. stenocephalus, (C. septentrionalis (C. barbatus group, C. blochi, C. acutus)) is defined by the presence of odontodes on infraorbitals and preopercle, and by an angled free border of opercle. Another clade, (C. flaveolus (C. arcuatus (C. punctatus group (C. triseriatus (C. garbei (C. aeneus (C. eques (C. rabauti group + Brochis)))))))) is defined by the lack of odontodes on opercle, homoplasic with Corydoras hastatus group. Some classification proposals are discussed.

Objectives of thesis: 1.To evaluate the effect of pH and total hardness of water on the development of eggs in aquarium fish Corydoras paleatus, Corydoras paleatus albin, Tanichthys albonubes, Tanichthys albonubes gold, Aphyosemion gardneri, Aphyosemion gardneri gold. 2.Recommendation of optimal values of total hardness of water and pH for successful development of eggs for each monitored aquarium fish. Based on published data in available literature sources and on own experiments, it was evaluated how total water hardness (CM) and pH influences development of aquarium fish eggs of Aphyosemion gardneri, Aphyosemion gardneri gold, Tanichthys albonubes, Tanichthys albonubes gold, Corydoras paleatus, Corydoras paleatus albin.

Defensive mechanisms vary widely in the animal kingdom ranging from physical defenses like spines to chemical defenses such as toxins. Toxins in these secretions and tissues can fluctuate from enzymes to lipids to uncharacterized chemicals. Next generation -omics technology and mass spectrometry are extremely important in analyzing these samples because of their ability to distinguish minute amounts of toxic substance within a complicated sample. The goal of this experiment was to look at secretions and tissues from Corydoras sterbai. All samples in this study were proteolyzed using a mixture of Trypsin and Lys-C, fractionated, and run through nanoLC-MS/MS analysis using an Orbitrap Fusion™ Tribrid™ mass spectrometer. Using guanidine hydrochloride as a denaturant, a total of 420 database peptide matches were discovered in the secretions and up to 777 database peptide matches among the tissues. Proteins of interest found in both the secretion and in the hypothesized gland include members of the prostaglandin synthesis pathway, phospholipases, and peroxiredoxins. It is theorized that C. sterbai uses its serrated rays in addition to these proteins to defend themselves from would be predators.