There are a significant number of microwave applications, including active antenna arrays, wireless communication systems, navigational applications, etc., where improvement of such qualities as manufacturing costs, size, weight, power consumption, etc. is still on the agenda of todays RF design. In order to meet these requirements, new technologies must be actively involved in fabrication of RF components with improved characteristics. One of such fabrication technologies is called LIGA, used before primarily in fluidics, photonics, bioengineering, and micromechanics, and only recently receiving growing attention in RF component fabrication. One of the RF components suffering limitations in performance due to limitations in fabrication capabilities is the compact single metal layer (SML) coupled-line 3-dB coupler, also called hybrid, required in some applications thanks to its ability to divide power equally and electrically isolate the output from the input. In todays practical edge-coupled SML coupler designs, the level of coupling is limited by the capabilities of the photolithographic process to print the coupled lines close enough for tight coupling and it is usually no tighter that 8 dB. A promising way to overcome this limitation is increasing the area of metallic interface of the coupled lines, thus increasing the mutual capacitance of the lines, and inherently the coupling between them. This should be preferably done with keeping the coupler compact with respect to the footprint area, which is attained by making taller conductors, i.e. employing the third dimension. In contrast with previously used RF component fabrication processes, LIGA is the technology that allows the designer to explore the third dimension and build tall conductors while being also able to use small features. When the two-dimensional edge-coupled SML couplers are extended into the three-dimensional structures, they rather become the side-coupled SML couplers. Tall-conductor coupled lines have been characterized in this work to reveal their dependence on their geometry and a 3-dB SML coupler with tall conductors has been developed and fabricated using LIGA at the Institute for Microstructure Technology (IMT), Karlsruhe, Germany. The simulation and measurement results demonstrate the potentially superior performance of LIGA couplers, and the promising capabilities of LIGA for fabrication of RF microstructures.

The development and fabrication of silicon-based phononic band gap crystals has been gaining interest since phononic band gap crystals have implications in fundamental science and display the potential for application in engineering by providing a relatively new platform for the realization of sensors and signal processing elements. The seminal study of phononic band gap phenomenon for classical elastic wave localization in structures with periodicity in two- or three-physical dimensions occurred in the early 1990’s. Micro-integration of silicon devices that leverage this phenomenon followed from the mid-2000’s until the present. The reported micro-integration relies on exotic piezoelectric transduction, phononic band gap crystals that are etched into semi-infinite or finite-thickness slabs which support surface or slab waves, phononic band gap crystals of numerous lattice constants in dimension and phononic band gap crystal truncation by homogeneous mediums or piezoelectric transducers. The thesis reports, to the best of the author's knowledge, for the first time, the theory, design methodology and experiment of an electrostatically actuated silicon-plate phononic band gap quasi-crystal architecture, which may serve as a platform for the development of a new generation of silicon-integrated sensors, signal processing elements and improved mechanical systems. Electrostatic actuation mitigates the utilization of piezoelectric transducers and provides action at a distance type forces so that the phononic band gap quasi-crystal edges may be free standing for potentially reduced anchor and substrate mode loss and improved energy confinement compared with traditional surface and slab wave phononic band gap crystals. The proposed phononic band gap quasi-crystal architecture is physically scaled for fabrication as MEMS in a silicon-on-insulator process. Reasonable experimental verification of the model of the electrostatically actuated phononic band gap quasi-crystal architecture is obtained through extensive dynamic harmonic analysis and mode shape topography measurements utilizing optical non-destructive laser-Doppler velocimetry. We have utilized our devices to obtain fundamental information regarding novel transduction mechanisms and behavioral characteristics of the phononic band gap quasi-crystal architecture. Applicability of the phononic band gap quasi-crystal architecture to physical temperature sensors is demonstrated experimentally. Vibration stabilized resonators are demonstrated numerically.

A three dimensional time-dependent coupled ecosystem model is applied to Izmir Bay for the first time. Delft3D modelling suite&rsquo / s FLOW and ECO modules are adapted and tuned for the region. A reference model with a time frame of three years is produced that represents the current physical and biogeochemical status of the bay. Model skill assessment methods are used as a measure of model performance and to address the shortcomings of it. The hydrodynamics model is able to produce physical features in terms of seasonality and spatial distribution within reasonable ranges, whereas the ecosystem model has certain discrepancies which can be reduced with improved quality of model inputs, such as open boundary conditions, and fresh water and nutrient fluxes. The reference model is used as a tool with predictive capacity to assess the ecosystem response of the bay to possible changes it may undergo in the future. Five nutrient enrichment/reduction scenarios are constructed to predict the reactions of the bay to changing external inputs of DIN and PO4. Results suggest that both physical and biogeochemical properties of the bay show strong horizontal gradients between outer and inner regions in which both natural and anthropogenic influences are effective. It is revealed that Outer bays are mostly occupied by waters originating from the oligotrophic Aegean Sea, while eutrophicated inner regions are mainly controlled by local influences such as increased fresh water inputs and excessive wastewater discharges. Results of the nutrient enrichment/reduction scenarios suggest that the N-limited Inner and Middle bays and the P-limited Outer bays, give contrasting reactions to changes in inputs of DIN and PO4 such that the former is more sensitive to DIN input whereas the latter is more sensitive to PO4 input. Due to the existence of these two contrasting environments in the bay, availability of one nutrient is dependent on the availability of the other, therefore treatment of both should be considered in parallel. Among the scenarios tested in this study, the best possible option to reduce eutrophication in Izmir Bay is to prevent the increase of PO4 input and to reduce the DIN input simultaneously. These outcomes are aimed to provide a scientific insight for coastal policy makers and environmental managers on how changes in anthropogenic influences can impact the marine ecosystem of the bay.

G protein-coupled receptors (GPCRs) are known to exist as oligomers, but there is much uncertainty over the oligomeric size, the number of interacting G proteins and the stability of that interaction. The present approach to these questions has been threefold. Monomers of the M2 muscarinic receptor were purified from Spodoptera frugiperda (Sf9) cells and reconstituted in phospholipid vesicles, where they spontaneously formed tetramers. The size of the reconstituted complex was determined from its electrophoretic mobility after cross-linking and inferred from a quantitative, model-based assessment of cooperative effects in the binding of two muscarinic antagonists: N-methylscopolamine and quinuclidinylbenzilate. Binding of the agonist oxotremorine-M to receptor reconstituted with purified G proteins revealed at least three classes of sites that interconverted from higher to lower affinity upon the addition of guanylylimidotriphosphate (GMP-PNP). The binding properties resemble those of muscarinic receptors in myocardial preparations, thereby implying the existence of tetramers in native tissues. G proteins that copurify with the M2 receptor from cardiac membranes also were found to exist as oligomers, some of which contain both alpha(o) and alpha(i2), and the purified complexes contained receptor and G protein in near-equal amounts. A tetrameric receptor implies a tetramer of G proteins, a conclusion that is supported by the distribution of sites between different states identified in the binding of [35S]GTPgammaS to the purified complex. Covalent adducts of a GPCR fused to a Galpha-subunit provide a model system in which the relationship between receptor and G protein complex is defined with respect to stability and composition. Such a fusion of the M2 receptor and Galpha(i1) underwent a cleavage near the amino terminus of the alpha-subunit, however, flagging the likelihood of similar effects in other such adducts. Truncation of the amino terminus prior to fusion generated a stable product that revealed GMP-PNP-sensitive, biphasic binding of oxotremorine-M and noncompetitive interactions between N-methylscopolamine and quinuclidinylbenzilate. A covalent RG complex therefore exhibits the functional properties of M2 receptors in native systems. These observations are consistent with the notion that signaling through the M2 receptor occurs via cooperative interactions within a stable complex that comprises four receptors and four G proteins.

Landfills are considered to be one of the major sources of anthropogenic methane (CH4) emissions in the environment. A landfill biocover system optimizes environmental conditions for biotic CH4 consumption that controls the fugitive and residual emissions from landfills. A compost material has more oxidation potential in comparison to any other material due to its high porosity, organic content, free flux for gases and water holding capacity. Thermal, hydraulic, bio – chemical and mechanical (THMCB) properties are important factors that can significantly affect the performance of biocover material with regards to CH4 oxidation potential as well as structural stability. Technical data on the thermal, hydraulic and mechanical (THM) properties of compost based biocover materials are quite limited. Hence, a detailed experimental program has been carried out at the University of Ottawa to study the THM properties and behaviour of compost biocover material by conducting experimental tests on small compost samples as well as by performing column experiments. The test results indicate that lower water content (dry of optimum for compaction curve) shows more free air space (FAS) in comparison to higher water content. The compost has almost the same shear strength for various initial water contents and dry unit weights; however, it settles and swells more at higher water content than lower water content per mechanical test results. The thermal and hydraulic properties of compost are a function of the compaction degree in addition to various other parameters. It is also found that the THM properties of compost are strongly coupled and the degree of saturation greatly affects the FAS.

No description available.

No description available.

Esta Tesis está dirigida al análisis teórico y experimental de problemas acoplados Termo-Hidro Mecánico (THM) que se desarrollan en formaciones geológicas profundas destinadas al almacenamiento de residuos radiactivos de alta actividad. En las últimas décadas, han sido estudiadas las formaciones arcillosas para ser utilizadas como barreras geológicas debido a su reducida conductividad hidráulica. La degradación de las rocas arcillosas producida por efectos de temperatura y por efectos de variación en el grado de saturación, es un factor de fundamental importancia, que es actualmente investigado en ensayos in situ a gran escala, como así también en ensayos de laboratorio. En ésta tesis, la roca Opalinus Clay ha sido ampliamente caracterizada mediante ensayos de laboratorios. Desde un punto de vista macro-estructural se ha obtenido la curva de retención de agua, conductividad hidráulica, resistencia y deformación. El análisis micro-estructural está enfocado a la caracterización mineralógica obtenida por difracción de rayos X, la distribución del tamaño de los poros determinada por porosimetría de mercurio (MIP) y microscopía electrónica (SEM). La tesis describe también un ensayo in situ de calentamiento diseñado para analizar la interacción entre la barrera de ingeniería (bloques de bentonita compactada) y la barrera geológica (Opalinus clay). Esta interacción ha sido analizada a través de simulaciones numéricas realizadas con el código de elementos finitos CODE_BRIGHT. Una célula termo-hidráulica fue especialmente diseñada para observar el comportamiento THM de la roca en condición drenada y no drenada, a través de pulsos de calor. Parámetros térmicos e hidráulicos de la roca fueron determinados por retro análisis a través de simulaciones numéricas realizadas con CODE_BRIGHT. Desde el punto de vista mecánico, un modelo constitutivo ha sido formulado en 3D e implementado en CODE_BRIGHT con el objetivo de reproducir el comportamiento mecánico anisótropo y rotura frágil de las rocas arcillosas. El modelo es formulado en un marco viscoplástico y considera la resistencia y deformabilidad de la matriz y de las juntas. El criterio de falla de la matriz y de las juntas es definido por superficies de fluencias hiperbólicas en el espacio de tensiones p-J y τ−σ, respectivamente. El comportamiento frágil de las rocas arcillosas es simulado por un reblandecimiento isótropo y cinemático definido en términos de trabajo de deformación plástico. El modelo constitutivo ha sido calibrado mediante ensayos triaxiales de laboratorio realizados en especimenes con diferentes ángulos de buzamiento. El modeloconstitutivo anisótropo ha sido aplicado a la simulación numérica en 3D de un ensayo de calentamiento in-situ. Una simulación numérica en 3D de un ensayo de ventilación in-situ realizado en un micro-túnel sin recubrimiento ha sido realizada para reproducir el brusco cambio de permeabilidad por efectos de secado de la roca. En este caso, un modelo hidráulico que considera la apertura de las juntas por efectos de secado ha sido implementado para reproducir los cambios de permeabilidad en excavaciones subterráneas. / This thesis deals with the theoretical and experimental analysis of the coupled Thermo- Hydro-Mechanical (THM) processes developed in geological formations suitable for the repository of radioactive waste of high activity. In the last decades, the argillaceous formations have been studied to be used as geological barriers, due to its reduced hydraulic conductivity. The degradation of clay shales induced by temperature and saturation effects is an important factor which is currently being investigated in large scale in situ tests as well as in laboratory studies. In this thesis, the Opalinus clay rock has been widely characterized by means of laboratory tests. From a macro-structural point of view, the water retention curve, hydraulic conductivity, strength and deformability parameters have been determined. The micro-structural analysis is focused to the mineralogical characterization obtained by means of X ray diffraction, pore size distribution (PSD) determined by means of mercury intrusion porosimetry (MIP) and scanning electronic microscopy (SEM). The thesis describes also a large scale heating in situ test designed to analyze the interaction between the engineer barrier (compacted bentonite blocks) and by the geological barrier, (Opalinus clay). This interaction has been analyzed by means of numerical simulations performed with the finite element code CODE_BRIGHT. A thermo hydraulic cell was specially designed to observe the coupled THM behaviour of the clay shale rock under drained and undrained conditions by means of heat pulses. Thermal and hydraulic parameters of rock were determined by means of back-analysis performed with the help of CODE_BRIGHT. In order to reproduce the anisotropic and brittle behaviour of the clay shale, a 3D mechanical constitutive model has been formulated and implemented in CODE_BRIGHT. The constitutive model is formulated in a viscoplastic framework and it considers the strength and deformability of both matrix and discontinuities (joints). The failure criterion of the matrix and the joints is defined by means of hyperbolic yield surfaces in the p-J and τ-σ stress space, respectively. The brittle behaviour of clay shale is simulated by means of isotropic and kinematic softening defined in terms of a workhardening criterion. The anisotropic constitutive model has been calibrated against triaxial laboratory tests performed on specimens with a main family of discontinuities having different dip angles. The constitutive model has been applied to a 3D numerical simulation of an "in-situ" heating test. A 3D numerical simulation of a ventilation test performed in an unlined micro tunnel was also performed in order to reproduce the changes of the rock permeability by drying effects. In this case, a hydraulic model able to consider the changes in joint thickness by drying effects has been developed to reproduce the changes of permeability in underground excavations.

En los últimos años, el análisis numérico de problemas acoplados, como los procesos de degradación de materiales y estructuras relacionados con los efectos medioambientales, ha cobrado especial importancia en la comunidad científica de la mecánica del hormigón. Problemas de este tipo son por ejemplo el ataque químico, el efecto de altas temperaturas o la retracción por secado.Tradicionalmente, los análisis acoplados existentes en la literatura se han realizado a nivel macroscópico, considerando el material como un medio continuo y homogéneo. Sin embargo, es bien conocido que el origen de la degradación observada a nivel macroscópico, a menudo es debida a la interacción entre los áridos y el mortero, sobre todo cuando se dan cambios de volumen diferenciales entre los dos componentes. Esta es la razón por la que el análisis mesomecánico está emergiendo como una herramienta potente para estudios de materiales heterogéneos, aunque actualmente existen escasos modelos numéricos capaces de simular un problema acoplado a esta escala de observación.En esta tesis, la aplicabilidad del modelo meso-mecánico de elementos finitos, desarrollado en el seno del grupo de investigación durante los últimos quince años, se extiende al análisis de problemas acoplados higro-mecánicos y químico-mecánicos, con el fin de estudiar la retracción por secado y el ataque sulfático externo en muestras de hormigón. La generación numérica de mesogeometrías y mallas de elementos finitos con los áridos de mayor tamaño rodeados de la fase mortero se consigue mediante la teoría de Voronoï/Delaunay Adicionalmente, con el fin de simular las principales trayectorias de fisuración, se insertan a priori elementos junta de espesor nulo, equipados con una ley constitutiva basada en la mecánica de fractura no lineal, a lo largo de todos los contactos entre árido y matriz, y también en algunas líneas matriz-matriz.La aportación principal de esta tesis es, conjuntamente con la realización de análisis acoplados sobre una representación mesoestructural del material, la simulación no solo de la formación y propagación de fisuras, sino también la consideración explícita de la influencia de éstas en el proceso de difusión.Los cálculos numéricos se realizan mediante el uso de los códigos de elementos finitos DRAC y DRACFLOW, previamente desarrollados en el seno del grupo de investigación, y acoplados mediante una estrategia staggered. Las simula-ciones realizadas abarcan, entre otros aspectos, la evaluación del compor-tamiento acoplado, el ajuste de parámetros del modelo con resultados experimentales disponibles en la bibliografía, diferentes estudios del efecto de los áridos en la microfisuración inducida por el secado y las expansiones debidas al ataque sulfático, así como el efecto simultáneo de los procesos gobernados por difusión y cargas de origen mecánico. Los resultados obtenidos concuerdan con observaciones experimentales de la fisuración, el fenómeno de spalling y la evolución de las deformaciones, y muestran la capacidad del modelo para ser utilizado en el estudio de problemas acoplados en los que la naturaleza heterogénea y cuasi-frágil del material tiene un papel predominante.

Seven transmembrane (7-TM) G protein coupled receptors (GPCR) constitute the largest family of integral membrane proteins in eukaryotes with more than 1000 members and encoding more than 2% of the human genome. These proteins play a key role in the transmission and transduction of cellular signals responding to hormones, neurotransmitters, light and other agonists, regulating basic biological processes. Their natural abundance together with their localization in the cell membrane makes them suitable targets for therapeutic intervention. Consequently, GPCR are proteins with enormous pharmacologic interest, representing the targets of about 50% of the currently marketed drugs. The current limitations in the experimental techniques necessary for microscopic studies of the membrane as well as membrane proteins emerged the use of computational methods and specifically molecular dynamics simulations. The lead motif of this thesis is the study of GPCR by means of this technique, with the ultimate goal of developing a methodology that can be generalized to the study of most 7-TM as well as other membrane proteins. Since the bovine rhodopsin was the only protein of the GPCR family with a known threedimensional structure at an atomic level until very recently, most of the effort is centered in the study of this receptor as a model of GPCR.The scope of this thesis is twofold. On the one hand it addresses the study of the simulation conditions, including the procedure as well as the sampling box to get optimal results, and on the other, the biological implications of the structural and dynamical behavior observed in the simulations. Specifically, regarding the methodological aspects of the work, the bovine rhodopsin has been studied using different treatments of long-range electrostatic interactions and sampling conditions, as well as the effect of sampling the protein embedded in different one-component lipid bilayers. The binding of ions to lipid bilayers in the absence of the protein has also been investigated. Regarding the biological consequences of the analysis of the MD trajectories, it has been carefully addressed the binding site of retinal and its implications in the process of isomerization after photon uptake, the alteration a group of residues constituting the so-called electrostatic lock between helices TM3 and TM6 in rhodopsin putatively used as common activation mechanism of GPCR, and the structural effects caused by the dimerization based on a recent semi-empirical model. Finally, the specific binding of ions to bacteriorhodopsin has also been studied. The main conclusion of this thesis is provide support to molecular dynamics as technique capable to provide structural and dynamical informational about membranes and membrane proteins, not currently accessible from experimental methods). Moreover, the use of an explicit lipidic environment is crucial for the study the membrane protein dynamics as well as for the protein-protein and lipidprotein interactions.