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No description available.

No description available.

An Enzyme-Linked Immunosorbent Assay (ELISA) was developed which detected slug antigens in postmortem gut analysis of carabid beetles. The ELISA was used to identify beetles which fed on slugs in three fields of oilseed rape and winter wheat in the Tyne valley, Northumberland. Generalist species such as Harpalus rufipes, Pterostichus melanarius, Pterostichus nladidus, Anlara silnilata and Nebria brevicollis fed on slugs in the field. Molluscan specialists such as Carabus violaceus and Cychrus caraboides also fed on slugs in the field. Laboratory studies indicated that many large and medium sized carabids were able to predate small slugs. Some beetle species did not eat slugs but exposure to the beetles increased slug mortality. Therefore, postmortem investigations may underestimate the impact that carabids exert on slugs as they do not measure the number of slugs killed. Slug mucus affected the locomotory activity of generalist and specialist beetle species. Beetles foraged longer, covered greater distances, made more turns, walked slower and spent more time stationary on soil covered in slug mucus compared to control areas. Abax parallelepipedus, P.melanarius, Pterostichus niger and H.rufipes all reduced slug damage to a chinese cabbage crop in a miniplot experiment compared with unprotected plots. However, these differences were not significant. A.parallelepipedus was most effective at reducing slug damage to the chinese cabbage but was rare in arable land. H.rufipes was least effective at reducing slug damage but was abundant in arable land in both years of the study. A high proportion of H.rufipes beetles fed on slugs in the field. None of these four species occurred at densities in the field which reduced slug damage in the miniplot experiment.

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No description available.

No description available.

Artificial diet bioassays were carried out to investigate the impact of GNA and Con A upon the development of L. oleracea larvae. GNA, at 2 % of total dietary protein, exerted a significantly detrimental effect upon larval development, growth and consumption, with little effect upon survival. Con A was shown to be the more toxic of the 2 lectins. When tested at concentrations of 2.0 %, 0.2 % and 0.02 %, Con A caused a significant decrease in survival and larval development, and caused greater reductions in larval growth and consumption compared to GNA. The potential for GNA and Con A to exert insecticidal effects via binding to the brush border membrane (BBM) and peritrophic membrane (PM) of L. oleracea larvae was investigated. Con A, which specifically binds a-D-mannopyranoside and a-D- glucopyranoside residues, was shown to bind in vitro to the majority of BBM and PM proteins. In contrast GNA, which exhibits strict specificity for a(l,3) and a(l,6)-linked D-mannose residues, bound to only 5 BBMV and 2 PM proteins. In agreement, higher levels of Con A, compared to GNA, were shown to accumulate in larval gut tissue after feeding the proteins in vivo. Despite this both lectins were shown to have a similar ability to disrupt the digestive capacity of the larval midgut. GNA and Con A stimulated similar short term elevations in BBM enzyme and soluble trypsin activities and a long-term reduction in a-glucosidase activity. Increases in levels of trypsin activity in faecal material collected from lectin-fed larvae suggested that the proteins may act by disrupting mechanisms of enzyme recycling. Aminopeptidase, an abundant and avidly binding BBM protein (120 kDa), was identified as a major Con A binding species in L. oleracea. A 98 kDa GNA-binding BBM protein was purified and amino acid sequence data was obtained from digest polypeptides allowing oligonucleotide primers to be designed. Subsequent attempts to amplify (by PCR and RT-PCR) fragments containing coding sequence corresponding to the 98 kDa protein were unsuccessful. This was attributed to oligonucleotide degeneracy together with the low abundance and relatively large size of the protein. The potential for GNA and Con A to exert systemic effects upon I. oleracea was demonstrated by the detection of both lectins in the haemolymph of larvae exposed to experimental diets. GNA was detected in haemolymph of larvae exposed to experimental diet for just 2 hours. In contrast, no Con A was detectable in haemolymph extracted from larvae fed for 24 hours, although it was present in the haemolymph after 5 days of exposure to the diet. That GNA and Con A may act directly upon organs other than the insect gut was indicated by the detection of lectins in vivo in malpighian tubule and fat body tissue extracts. A significant reduction in haemocyte abundance in haemolymph samples extracted from lectin-fed larvae suggested that both GNA and Con A may also act by disrupting the immune system of L. oleracea.

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Quizalofop-p-ethyl is a herbicide introduced in the mid 1980's with apparent low toxicity and is readily degradable.Quizalofop-p-ethyl is a member of the aryloxyphenoxypropionate group of herbicides and is a postemergence herbicide used for pulses (including soybean) and vegetables. The aim of the project discussed in this study is to develop an analytical method for the sensitive and reliable determination of quizalofop-p-ethyl and quizalofop acid in soybean using HPLC. Soybean is chosen as a typical agricultural crop. It has 15 to 20 percent oil content and is a crop which has been successfully used with theis herbicide.Two different methods of extraction, i.e. the solvent-solvent extraction method and solid phase extraction method, and clean up are discussed in some detail. / Doctor of Philosophy (PhD)