M.S.

Wine vineyards in humid environments like the mid-Atlantic United States are characterized by vines that develop too much vegetative growth for optimum quality wine production. Cover crops, rootstocks and rootzone restriction were evaluated for their effect on vegetative and reproductive growth on Cabernet Sauvignon. Treatments were arranged in a strip-split-split plot arrangement with under-trellis cover crops (UTCC) compared to row-middle only cover crop combined with 1-m weed-free strips in the vine row as main plots. Rootstocks riparia Gloire, 420-A, and 101-14 were sub-plots, while sub-sub-plots comprised two treatments: vines were either planted in root-restrictive (RR), fabric bags (0.016 m3) at vineyard establishment (2006), or were planted without root restriction. All three factors were effective in suppressing vegetative development as measured by rate and extent of shoot growth, lateral shoot development, trunk circumference, and dormant pruning weights. Canopies of vines with UTCC and RR had reduced leaf layer values by approximately 21% and 23% compared to conventional controls. The principal effect of the UTCC and the RR treatments was a sustained reduction in stem (xylem) water potential. UTCC and RR caused significant 7 and 10% reductions in berry weight, compared to their conventional controls. Berry weights of vines grafted to riparia were greater than those of vines grafted to other rootstocks. Wine made from UTCC and RR treatments increased red wine color compared to herbicide UTGC and NRR, respectively. This study identified treatments that improve vine balance while simultaneously improving grape composition and potential wine quality. / Master of Science

The response of upland cotton (Gossypium hirsutum L.) to legume and small grain cover crop establishment, in-season nitrogen (N) rate, and fertilizer N placement was investigated in two experiments located in coastal plain Virginia and North Carolina. The first experiment examined 1) soil compaction and cotton yield response to strip-tillage compared to no-tillage with a precision planted tillage radish and 2) the influence of legume mix, rye, and legume mix/rye combination cover crops with four in-season nitrogen (N) rates applied to cotton on cover crop biomass, cover crop nutrient uptake, soil compaction, soil N cycling, petiole nitrate-N (NO3-N) during the first week of bloom, cotton lint yield, and fiber quality parameters over two years. Legume mix cover crops resulted in greater N uptake, soil NO3-N during the growing season, and lint yields compared to LMR, rye, and fallow treatments over both study years. Soil compaction and lint yields were not significantly different between strip-tilled and no-till with tillage radish treatments in either year. Relative lint yields after LM were maximized at 93% relative yield with 110 kg N ha-1 applied in-season while relative lint yields for cotton following LM with 0 kg N ha-1 applied reached 75%, measuring at least 9% higher than cotton following other cover crop treatments. The second experiment investigated the effect of five N rates (0, 45, 90, 135, and 180 kg N ha-1) and three placement methods (broadcast, surface banded, and injected) on lint yield, petiole nitrate-N (NO3-N), lint percent turnout, and fiber quality parameters. Nitrogen rate and placement had a significant effect on lint yield but only N rate affected petiole NO3-N concentration. It was estimated that injecting fertilizer N requires an N rate of 133 kg N ha-1 to achieve 95% relative yield while surface banded fertilizer N required a rate of 128 kg N ha-1 to produce 90% relative yield. A critical petiole NO3-N concentration threshold of 5,600 mg NO3-N kg-1 was calculated to reach 92% relative yield. Other agronomic management practices such as cover crop termination timing, cover crop species blends, and number of fertilizer N applications are of interest in order to develop better recommendations and promote conservation agricultural practices in coastal plain Virginia and North Carolina. / Master of Science / Upland cotton (Gossypium hirsutum L.) response to diverse species cover crop mixes, conservation tillage method, fertilizer N rate, and fertilizer N placement at side-dress was measured in two field studies conducted on the coastal plain soil in Virginia and North Carolina from 2016-2018. The objectives of the following research were to 1) examine the influence of two conservation tillage practices and four cover crop mixes on cover crop biomass production, soil compaction, cover crop nutrient uptake, soil N cycling, petiole nitrate (NO3-N) and cotton lint yield and 2) measure cotton performance in response to five N rate and three placement application methods. Legume mix (LM) cover crops contained more N in biomass, resulting in higher soil NO3-N during the growing season and higher lint yields at harvest compared to a legume mix and rye combination (LMR), rye, and fallow treatments. Soil compaction and lint yield were not significantly different between strip-tilled and no-till/tillage radish treatments in either year. Nitrogen rate and placement had a significant effect on lint yield but only N rate affected petiole NO3-N concentration. Injection of fertilizer N required an N rate of 133 kg N ha1 to achieve 95% relative yield while surface banded fertilizer N required a rate of 128 kg N ha-1 to produce 90% relative yield. A critical petiole NO3-N concentration threshold of 5,600 mg NO3-N kg-1 was also calculated to reach 92% relative yield. Future application of these results can include investigation of optimal N source for Virginia cotton production, best N placement method for cotton grown in high residue systems, and an economic analysis to determine optimum agronomic management for Virginia coastal plain cotton production.

To understand the impact of N management on harvestable cover crop systems, seven research trials compared: 1) standard intensive management (SIM) (both fall and spring N application), 2) No fall N, a single spring N application, and 3) Cover N (no N application) effects on winter barley (Hordeum vulgare L.) plant biomass (PB), plant N uptake (PNU), grain yield, residual soil nitrate (RSN), and ammonium (RSA). In general, at winter dormancy, SIM resulted in increased PB and PNU but not RSN or RSA. At cover crop termination; SIM and the No fall N practices increased PNU, and at harvesting stage; they produced higher grain yields than the Cover N practice with little significant effect on RSN or RSA values, under normal climatic conditions. While overall yields for the No fall N treatment were lower (8%) than SIM yields, partial net return was similar due to decreased fertilizer input. Nitrogen use efficiency (NUE) of soft red winter wheat (SRWW) can be improved by characterizing genotypes for NUE using canopy spectral reflectance [(CSR), a cheap, rapid and non-destructive remote sensing tool]. The other objectives of this study were to evaluate the predictive potential of vegetative reflection indices for wheat nitrogen use efficiency (NUE) by genotype and the appropriate stages of CSR sensing. An elite panel of 281 regionally developed SRWW genotypes was screened under low and normal N regimes in two crop seasons for grain yield, N uptake, nitrogen use efficiency for yield (NUEY) and nitrogen use efficiency for protein (NUEP). The best models incorporating CSR data at wheat heading explained a significant proportion of total variation in grain yield, N uptake, NUEY and NUEP. Based on the best linear unbiased predictor values, genotypes were ranked and grouped into quartiles and the most efficient and responsive genotypes were identified. A significant proportion of the genotypes with high NUEY under high N conditions also had high NUEY under N stress; however, this was not the case for NUEP. Similarly, a significant proportion of genotypes with high NUEY also had high NUEP under both normal and low N conditions. / Ph. D.

Grass clippings may be recycled onto nearby areas of turf as an alternative to disposal of clippings in landfills. In May, 1991, two field studies were initiated to evaluate grass clippings as a nitrogen (N) source for tall fescue (Festuca arundinacea, Schreb. Rebel’) turf, and to determine the maximum feasible loading rate of clippings onto tall fescue turf. Test plots in both studies received additional clippings from adjacent donor plots. In the first study, grass clippings alone were applied to 2.2 m² test plots at rates of 1 "plot equivalent harvest" (PEH), 2 PEH, and 4 PEH; where PEH is the amount of clippings harvested from 2.2 m² of donor plots. In the second study, test plots had clippings removed (Rem), clippings returned (Ret), Ret+1 PEH, and Ret+3 PEH. These four clipping treatments were arranged in a factorial combination with three fertilizer-N rates: low (73 kg N ha⁻¹ yr⁻¹), medium (147 kg N h⁻¹ yr⁻¹), and high (220 kg N h⁻¹ yr⁻¹). Donor plots received identical fertilizer-N applications as test plots. For both studies, N uptake generally showed a linear response to total N applied. Clipping-N produced roughly the same amount of growth and N uptake as the fertilizer-N, even as the rate of N uptake varied with time. In both studies, soil nitrate levels remained low until excessive clippings smothered the turf and caused a reduction in N uptake. Soil nitrate concentration tended to decrease with depth for all plots. Soil total Kjeldahl nitrogen (TKN) levels did not differ among treatments, suggesting that clipping-N was not being stored in the soil. At the end of both growing seasons, thatch accumulation was less than 12mm in all plots. In general, increased additions of clippings resulted in improved color, density, and growth. However, at the very high rates of added clippings, excess biomass caused turf thinning, chlorosis, and low growth. Thus, if grass clippings are applied at rates that are low enough to prevent these problems, they can be an effective N source for tall fescue. Furthermore, in order to dispose of the maximum amount of clippings, tall fescue should not be fertilized. / Master of Science

M.S.

Complete vineyard floor cover cropping and root pruning (RP) were evaluated for their ability to regulate excessive vegetative growth and improve berry and wine composition of ‘Cabernet Sauvignon’ (Vitis vinifera L.). Treatments were: tall fescue (Festuca arundinacea Shreb.) ‘KY-31’ and ‘Elite II’, hard fescue (Festuca ovina L.) ‘Aurora Gold’, perennial ryegrass (Lolium perenne L.), orchardgrass (Dactylis glomerata L.), and an under-trellis herbicide strip combined with KY-31fescue interrows. Compared to herbicide strip/non-root pruned (NRP), Elite II fescue reduced vine pruning weight (kg/vine) 28%, individual cane weight (g) 20%, and canopy leaf layer number 25%. KY-31 fescue/RP lowered vine pruning weights 29% compared to an 8% reduction in pruning weights of vines grown in herbicide strip/NRP plots from 2005 to 2010. KY-31 fescue produced the greatest biomass and stand density. With the exception of a yield reduction in vines grown with KY-31 fescue in 2006, cover crops minimally decreased grape yield. Yearly climatic variation had a greater effect on berry weight and composition (pH, TSS, TA) than did treatments. Limited treatment differences detected in chemical compounds by gas chromatography–mass spectrometry (GC-MS) analysis in wines made from treatment vines in 2010 were correlated to descriptive sensory terms. Cover crop water use, as evapotranspiration, determined by mini-lysimeter (ML), ranged from 3.28 mm/d for KY-31 fescue to 1.52 mm/d for herbicide-treated plots. In 2008, root biomass of vines grown on KY-31 fescue/RP was increased at the 60 to 80 and 80 to 100 cm soil depths compared to root biomass of KY-31 fescue/NRP vines at those depths. Cover crops minimally impacted vine water potential (ΨPD, Ψmd, Ψstem) and grapevine nitrogen levels relative to the herbicide strip, indicating that the grasses were not overly competitive with grapevines. Root pruning and complete vineyard floor cover crops favorably reduced grapevine vegetative growth, although treatment effects diminished over time, possibly in response to redistribution of grapevines’ roots and climatic variation at the site. / Ph. D.

This investigation was planned to study the new dormant spray, dinitro-ortho-cyclo-hexyl-phenol in relation to its use under Virginia conditions for the control of certain insects with which the Virginia orchardist has constantly to deal. The object of this study is to determine what affects the varying amounts of dinitro-ortho-cyclo-hexyl-phenol, either in oil or water sprays with different emulsifiers, has on penetration, injury and effectiveness. Conclusions which may be drawn from the work discussed in this thesis are: (1) That the 50 per cent goulac and 50 per cent bentonite emulsifier used at the rate of 1.25 pounds per 100 gallons is seemingly the best material among those tested. However, under the conditions in which trials were made this year, a dry form of dinitro-ortho-cyclo-hexyl-phenol mixed with an emulsifier containing bentonite and soy flour appears to be superior in preliminary tests, either in oil or water sprays, due to its better wedding power. (2) That injury which has been examined apparently occurs at the bud and enters the branch through the bud. (3) That the dinitro-ortho-cyclo-hexyl-phenol in oil and water sprays should be applied only in the dormant season, although no serious injury has followed its use in the plots used for this study even when the buds have pushed out leaves as much as one quarter of an inch. (4) That in the plots treated, the powder and water form of dinitro-ortho-cyclo-hexyl-phenol gave satisfactory control of Rosy aphis. (5) That time, care and the following of directions should not be sacrificed in the making up of the emulsions. / Master of Science

The diverse cropping system of eastern Virginia's coastal plain offers limited opportunity to establish winter annual cover crops (WCC) for nitrogen (N) scavenging. The winter fallow niche after double-crop or full-season soybean (Glycine max L. Merr.) encompasses the majority of acres left fallow. Our objective was to evaluate interseeded WCC N scavenging performance following soybean and N supplying capacity to subsequent corn (Zea mays L.). Field studies were conducted at four different locations in each of the two study years. The experimental design was split plot with cereal rye, hairy vetch, and RV mix WCC as main plots and ten fertilizer nitrogen (FN) rates in a factorial arrangement (0 and 45 kg FN ha-1 as starter; and 0, 45, 90, 135, and 180 kg FN ha-1 at sidedress) to corn as subplots. The highest N uptake for cereal rye at winter dormancy was 18 kg N ha-1, but the average was 6-7 kg N ha-1. At WCC termination average N uptake for cereal rye was 35 and 40 kg N ha-1 in 2013 and 2014, respectively. Average biomass dry matter (DM) at WCC termination for cereal rye, cereal rye + hairy vetch mix (RV mix), and hairy vetch was 2356, 2000, and 1864 kg ha-1 in 2013; and 2055, 2701, and 692 kg ha-1 in 2014, respectively. Average cereal rye N uptake was 35 kg N ha-1 in 2013 and 40 kg N ha-1 in 2014. Significant differences for residual soil nitrogen were most apparent for soil nitrate (NO3-N) at lower depths (15-30 and 30-60 cm) during WCC termination and in the upper 0-15 cm during corn growth stage (GS) V4 of both years. Corn grain yield plateau following hairy vetch WCC was 0.7 and 0.6 Mg ha-1 higher than when following cereal rye WCC at zero and 45 kg ha-1 starter FN, respectively. Average agronomic optimum FN rates (AONR) were 26 and 9 kg ha-1 lower following hairy vetch than cereal rye WCC at zero and 45 kg ha-1 starter FN, respectively. Estimated hairy vetch FN reductions by FN replacement and AONR difference methods were 48 and 18 kg FN ha-1 in plots receiving zero starter FN; and 58 and -43 kg FN ha-1 in plots receiving 45 kg ha-1 starter FN. In-season soil N tests did not offer adequate information in order to predict sidedress FN reductions. These findings suggest that cereal rye and RV mix have the potential to scavenge and conserve residual soil N and hairy vetch is more than capable to supply PAN to subsequent corn when interseeded into soybean. / Master of Science

Improving soil health in agricultural soils is vital as the effects of climate change and an increasingly affluent population are putting a strain on land resources. Conservation agricultural practices such as cover cropping and conservation tillage systems (e.g., strip tillage, minimal tillage, or no tillage) are implemented to improve soil properties, but soil health outcomes in Coastal Plain cropping systems have been inconsistent and may take decades or longer to occur. Improving soil health and agricultural prosperity in the region requires: a better understanding of the rate and magnitude of response in soil properties to different types of management; and a better understanding of the interaction between management types, soil health parameters, and crop yield. In this study, four tillage systems (in order of decreasing intensity: conventional tillage > strip tillage > minimal tillage > and no tillage) and three winter cover rotations (in order of decreasing expected biomass input: high-biomass cover crop > winter cash crop > and fallow) were tested in a split plot design. For the first objective of this study, bulk density, penetration resistance (as depth to root restrictive layer), total organic carbon, carbon stocks, saturated hydraulic conductivity, and yield were measured repeatedly over a seven-year period to better understand the longitudinal response of these properties to management. Bulk density and saturated hydraulic conductivity showed a greater temporal variation in the more intensive tillage practices (strip tillage and conventional tillage) in the top 5 cm. Depth to root restrictive layer was consistently highest in the deep tillage treatments (strip tillage and minimal tillage). The change in soil organic carbon concentration between 2018 and 2022 in the top 5 cm was numerically greater in the strip tillage (0.67%) and no tillage (0.68) than the conventional tillage was 0.18%. In 2022, the carbon stocks were significantly increased in all three conservation tillage treatments compared to conventional tillage. Yield was significantly increased by winter cover in the years that a legume/non-legume cover crop mix was implemented. For the second objective of this study, the effects of 6 years of management were evaluated for total organic carbon, active carbon, short-term carbon mineralization, aggregate stability, and crop yield. These parameters were then correlated to determine drivers in soil health outcomes. Total organic carbon was increased in the strip tillage treatment (1.13% in 2021 and 1.61 % in 2022) compared to conventional tillage (0.83% in 2021 and 1.09% in 2022) in the top 5 cm. In 2022, total organic carbon under cover crop (1.51 %) and cash crop (1.46 %) treatments were also significantly higher than the fallow (1.33 %) in the top 5 cm. Active carbon results followed a similar but weaker trend in both years. Percent aggregate stability was improved by minimal tillage (47%) in 2022 compared to conventional tillage (41%). The findings from the correlation analyses suggest that total organic carbon concentration, soil texture, and tillage were all prominent drivers in improving soil health outcomes. These results demonstrate the relatively slow response of many soil physical properties to management, highlight the need for consistent management to improve properties, and emphasize the need to take measurements at different times of the year. This information can be used to develop more sustainable and resilient cropping systems in the Coastal Plain. / M.S. / Centuries of intensive farming practices have degraded agricultural soils, which contributes to the challenge of feeding a growing and increasingly affluent global population. Therefore, understanding which farming practices reverse this degradation and improve soil health is vital for regions such as the southeastern Coastal Plain, where agriculture is a prominent industry. Soil health is defined as the ability of soil to support life sustainably. Conservation agricultural practices, such as planting crops over the winter and conservation tillage systems (where at least 30% of the soil surface is left undisturbed) are implemented to improve soil properties, but soil health outcomes in Coastal Plain farms have been inconsistent and may take decades or longer to occur. Therefore, the improvement of environmental health and agricultural prosperity in this region requires a better understanding of the patterns of change in soil properties in response to different types of management; and a better understanding of the factors that most influence soil health outcomes. This study evaluated the combination of four tillage systems of differing intensities, and three winter crop types on soil health outcomes. For the first objective of this study, soil physical properties, soil carbon, and crop yield were measured repeatedly over a seven-year period to quantify how these properties respond to management. The soil physical properties showed temporary improvement by more intensive tillage but reverted in the months after tillage. The soil carbon increased from 2018 to 2022 in the three least-intensive tillage treatments. Crop yield was significantly increased in years when there was a mix of crop species planted. For the second objective of this study, the effects of management on soil carbon, soil stability, and crop yield were evaluated after six years of management. These soil health parameters were then correlated to determine the most prominent factors influencing change in soil properties. Soil carbon and soil stability were both improved in least-intensive tillage treatments. The findings from the correlation analyses suggest that soil carbon concentration, soil texture, and tillage were all prominent factors in improving soil health outcomes. These results demonstrate the relatively slow response in many soil physical properties to management, highlight the need for consistent management to improve soil health, and emphasize the importance of taking measurements at different times throughout the year. Furthermore, this is information that can be used to develop more sustainable and resilient cropping systems in the Coastal Plain.