The rapid development of nanotechnology has caused concerns about nanoproducts on human health throughout their lifecycle. As part of the consortium NEPHH (nanomaterial related environmental pollution on human health through their life cycle, funded by EU-FP7), this project aimed to assess the potential effect of novel polymer-silicon composites on human health from a lifecycle perspective, focusing on in vitro toxicity of raw silica nanoparticles (SiNP) and dust nanoparticles (NP) released from silicon-based polymer composites. The main objectives were to characterise a group of amorphous SiNP and dust NP in water and cell culture medium; assess NP toxicity potential in in vitro models; and establish mode of SiNP action. The selection of SiNP of size 7-14 nm was based on their wide use in developing polymer nanocomposites. Dust NP were generated from mechanical processing of polymer composites made of polyamide-6 (PA6), polyurethane (PU) and polypropylene (PP), each incorporated with SiNP or 3 other different silicon reinforcement materials. The dispersion and size of NP in water and in cell culture medium were characterized using dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The chemical composition of NP was assessed by infra-red spectroscopy. NP were assessed in vitro for induction of membrane damage, intracellular reactive oxygen species (ROS), loss of cell viability, and cellular uptake by flow cytometry and confocal microscopy. In order to identify potential biomarkers for toxicity prediction, miRNA array and extracellular metabonomic assays were performed. The size of SiNP (10-100 µg/ml) ranged from ~200-500 nm in water and ~20- 500 nm in culture medium, indicating the presence of aggregates. The infra-red spectrum of SiNP dried from culture medium showed a slight difference as compared with that dried from water, indicating protein adsorption. SiNP induced acute ROS increase, cell membrane damage, and reduction in cell viability after 48 h in human lung carcinoma epithelial A549 cells, lung fibroblast MRC-5 cells and skin HaCaT keratinocytes. SiNP were up taken by all 3 cell types, and located in the cytosol. Six early ( < 48h) SiNP responsive miRNAs were identified in A549 cells. SiNP also induced early changes in metabolites including glucose, lactate, ethanol, phenylalanine, histidine and tyrosine. Dust NP generated from PA6 group materials were more toxic than those from other polymer composites when assessed at 25-100 µg/ml at 72 h in A549 cells. The results obtained from this study suggest that 1) both small and larger SiNP aggregates are taken up into the target cells; 2) conventional cytotoxicity assays combined with miRNA and metabonomic assays provide insight into the molecular mechanisms of the nanotoxicity; 3) metabonomics and miRNA assays can serve as robust tools for recognising sub-toxic dose-effect relationships; 4) the toxicity of dust NP from polymer composites depends on polymer type but not reinforcement materials. This study demonstrated the importance of lifecycle analysis as opposed to single stage analysis of novel materials. Further studies need to improve study design to enable interpretation of cytotoxicity in relation to NP size, physiochemical property and intracellular dose, and to simulate the health effect of polymer-silicon composites under more realistic scenarios.

In recent decades, society's concern about unsustainable development has grown steadily, while the desire to deal with the situation has also grown in connection with the concern and increased interest in the environment. The development of new concepts for systematic control and follow-up has also been steadily growing. The concepts developed are different environmental management systems, where the most accepted management system is ISO 14001. This has resulted in the development of tools with specific goals, in this study's focus, the Life Cycle Analysis. With the above background as a basis, this study aims to investigate and develop improvement measures in an organization with a focus on life cycle analysis and process mapping. The theory of the study is based on the ISO standards and in literature, while relevant models for the study are presented. The method through the writing of the study has been based on interviews, observations and literature studies and that validity, reliability, generalizability, and ethical positions have been kept in mind throughout the study to ensure a high quality.  The completed study came up with two suggestions for improvement: implement a process map for work with life cycle analysis and implement a flowchart for the decision process for products on which an LCA is to be performed. In the penultimate chapter of the study, the improvement proposals are presented which can hopefully make the organization's current working methods more efficient. / De senaste decennierna har samhällets oro för den icke-hållbara utvecklingen vuxit stadigt, samtidigt som även viljan att hantera situationen vuxit i samband med oron och ökat intresset för miljön. Utvecklingen av nya koncept för systematisk styrning och uppföljning har även de varit stadigt växande. Koncepten som utvecklats är olika miljöledningssystem, där det mest accepterade ledningssystemet är ISO 14001. Detta har resulterat i att verktyg med specifika mål utvecklats, i denna studies fokus, Livscykelanalysen. Med bakgrunden ovan som underlag, syftar denna studie till att undersöka och utveckla förbättringsåtgärder i en organisation med fokus på livscykelanalys och processkartläggning. Studiens teori grundar sig i ISO-standarderna och i litteratur samtidigt som relevanta modeller för studien presenteras. Metoden genom studiens skrivande har baserat sig på intervjuer, observationer och litteraturstudier samt att validitet, tillförlitlighet, generaliserbarhet och etiska ställningstaganden hafts i åtanke genom hela studien för att säkerställa en god kvalitet.  Den genomförda studien kom fram till två förbättringsförslag: implementera en processkarta för arbete med livscykelanalys samt implementera ett flödesschema för beslutsprocess vid produkter som en LCA ska utföras på. I studiens näst sista kapitel presenteras förbättringsförslagen som förhoppningsvis kan effektivisera organisationens nuvarande arbetssätt.

No description available.

Pilot Induced Oscillations (PIO) are described as pilot-aircraft dynamic couplings which can lead to instability in an otherwise stable system. Previous and ongoing research has attempted to explain, predict, and avoid such oscillations. In contrast to other research, this effort backs away from pilot models and PIO avoidance and focuses on the characteristics of the pilot before, during, and after a PIO. Often, PIO''s can be explained by limit cycles occurring in a non-linear system where the non-linearities cause a sustained, constant amplitude oscillation. The primary instigators in such a PIO are usually a non-linear element (i.e. rate limit saturation) and a trigger event (i.e. pilot mode switching or increased pilot gain). By performing analysis in the frequency domain, determining such oscillations becomes easier. Using spectrograms and power spectral density functions, the frequency content of a signal in the pilot-aircraft system can also be investigated. An F-14 flight test was recently performed where the hydraulic system was modified to determine the feasibility of trying to recover the aircraft (land on carrier) during such an extreme hydraulic failure. During testing, a severe PIO occurred because of the tight tracking task used during aerial refueling. While performing spectrograms and power spectral analysis, an increase in power concentration at the PIO frequency was observed. With a linear approximation of the F-14 aircraft dynamics, a closed-loop system containing the aircraft, actuator, and pilot dynamics is developed so that limit cycle analysis can be performed. With stable limit cycle solutions found possible, a pilot-in-the-loop simulation is performed to verify the pilot model used in limit cycle analysis. Using the flight test data, limit cycle analysis, and pilot-in-the-loop simulation, a connection between variation in pilot behavior and PIO predicted by the increase in power concentration is investigated. The resulting connection showed that an increase in pilot gain along with a transition from observing pitch attitude to pitch rate are the possible trigger events causing the PIO. The use of spectrograms as a PIO predictor is shown to be possible, provided the necessary calculations can be completed in real-time. / Master of Science

The work performed in this thesis involves the study of human hip joint kinematics and load analysis. Such analyses are very useful for investigating mobility and natural functionality as well as the variation in motion due to replacement implants. The objective of this study is to design, build and testing of a universal human joint simulator that is configurable to hold several human joints and easily programmable to create the required motion. This was performed by creating a Stewart Platform, which is capable of moving in all six degrees of freedom; the maximum number needed by any human joint. Many specific human joint simulators are available on the market for simulating all major human limbs. These are used for wear testing replacement joints by using high load repetitive motion. These systems have a predetermined limit degree of movement and are very expensive; if one wanted to emulate another joint, one would have to purchase a whole new system. This novel system compromises of a three-phase power supply, Control Area Network with six actuators and drivers, a force reading clamp with strain gauges and data logger. A user friendly computer program was developed that is able to derive joint movement data from two inputs and replicating the movement by driving the platform, as well as recording force and displacement data from the joint. The product would be marketed towards biomechanical researchers and implant designers. Verification of this system was performed by simulating the human hip joint. A known combination of kinematic and force data were inputted into the system for nine different types of activities. The resultant force and joint centre displacement was then compared to see how well the system perform in comparison to the inputted data from a previous study. The outcome of this project is a fully functional machine and configurable program that can create movement data at varying speeds and body weights; which is also able to drive the human joint simulator. The design also costs a fraction of any industrial joint simulator. It is hoped that the simulator will allow easier study of both the kinematics and load analysis within the human joints, with the intent on aiding investigation into mobility and functionality; as well as variation in motion caused by a replacement implant.

Many tools have been developed to assess global, national or regional sustainable development policies. However, as governments develop sustainable policies, individuals must also feel empowered to affect their personal impact on the planet. This thesis integrates three sustainability concepts that lend themselves to individual sustainability: The natural step, life cycle assessment, and the ecological footprint. TNS serves to provide the meaning and substance toward sustainable development. LCA helps provide the framework for assessing sustainability. The EF calculator determines the driving components and measures the qualitative decisions made through TNS and LCA. From the analysis of the household footprint calculator a simplified footprint calculator was developed to assist individuals and communities in setting benchmarks and goals as they move away from over-consumption and towards a sustainable lifestyle.

A indústria da construção civil é uma das principais fontes emissoras de poluição devido ao alto consumo de energia em seu ciclo de vida. Além da energia empregada na construção do edifício, durante sua operação, diversos sistemas também a utilizam, entre os quais se destaca, o sistema de condicionamento de ar. O consumo de energia por esse sistema está relacionado, entre outras coisas, com a temperatura do ar no ambiente externo e a requerida internamente pelo edifício. As fachadas são os elementos pelos quais se dá a maior parcela de transferência de calor do ambiente externo para o interno em edifícios altos. Assim, o tipo de fachada influi no consumo de energia no ciclo de vida dos edifícios e, conseqüentemente, contribui também para a emissão de CO2 pelos edifícios, pois a emissão deste gás está diretamente ligada ao consumo de energia. Desse modo, o objetivo deste trabalho é contribuir para o desenvolvimento da metodologia de avaliação das emissões de CO2 geradas durante o ciclo de vida das fachadas de edifícios de escritório. Os resultados, para os parâmetros considerados neste trabalho, mostram que as fachadas em structural glazing com vidro incolor são as que mais emitem CO2 ao longo de seu ciclo de vida, seguidas das fachadas em alvenaria e revestidas com painéis de alumínio composto ou ACM (Aluminium Composite Material), fachadas em structural glazing com vidro refletivo e fachadas em alvenaria revestidas com argamassa. Por fim, realizou-se uma análise para se avaliar a incerteza dos resultados deste estudo. / The construction industry is one of the main sources of pollution due to high energy consumption in its life cycle. Besides the energy used in the construction of the building during its operation, many systems also use, mainly, the air conditioning system. The energy consumption of the air conditioning system is related, among others things, with the outdoor air temperature and the indoor required air temperature. The façades are the elements in which the transference of heat happens from the outdoor environment to the indoor environment in high buildings. So, the façade typologies contribute to the energy consumption in the life cycle of the buildings and to CO2 emissions, because the emission of this gas is linked directly to the energy consumption. The objective of this work is to contribute to the development of evaluation methodology of CO2 emissions generated during the life cycle of the façades of office buildings. The results for the parameters considered in this work show that the façades in structural glazing with colorless glass are those that emit more CO2 throughout its life cycle, followed by the façades in masonry and coated with ACM (Aluminium Composite Material), façades in structural glazing with reflective glass and façades in masonry coated with mortar. Finally, an analysis was made to evaluate the results uncertainty.

Nanotechnology research and its commercial applications have experienced an exponential rise in the recent decades. Although there are a lot of studies with regards to toxicity of nanoparticles, the exposure to nanoparticles, both in terms of quality and quantity, during the life cycle of nanocomposites is very much an unknown quantity and an active area of research. Unsurprisingly, the regulations governing the use and disposal of nanomaterials during its life cycle are behind the curve. This work aims to assess the quantity of nanoparticles released along the life cycle of nanocomposites. Machining operations such as milling and drilling were chosen to simulate the manufacturing of nanocomposites parts, and impact testing to recreate the end-of-life of the materials. Several studies have tried to simulate different release scenarios, however these experiments had many variables and in general were not done in controlled environments. In this study, a reliable method was developed to assess the release of nanoparticles during machining and low velocity impact of nanocomposites. The development and validation of a new prototype used for measurement and monitoring of nanoparticles in a controlled environment is presented, as along with release experiments on different nanocomposites. Every sample tested was found to release nanoparticles irrespective of the mechanical process used or the type of material tested. Even neat polymers released nanoparticles when subjected to mechanical forces. The type of matrix was identified to play a major role on the quantity of nanoparticles release during different process. Thermoset polymers (and especially polyester) were found to release a higher number concentration of particles, mainly due to their brittle properties. A polyester sample was found to release up to 48 times more particles than a polypropylene one during drilling. The nanofiller type and percentage used to reinforce the polymer is also a key point. For example, the addition of 2 wt.% of nano-alumina into polyester increases the number concentration of particles by 106 % following an impact. The nanofiller chosen and its quantity affect the mechanical properties and machinability of the composites and therefore its nanoparticles release potential. The mechanical process and the process parameters chosen were also found to be crucial with regards to the nanoparticles released with different trends observed during drilling and impact of similar materials. Finally, thermal ageing of nanocomposites increases the number concentration of nanoparticles released (by 8 to 17 times after 6 weeks).

According to the USDA Economic Research service, farm-level prices are on the decline. This decline in prices particularly hurts smaller scale operators with many needing to rely on off-farm income in order to ensure they remain in operation. This thesis studies two problems of key interest to the Southeast region and the State of Kentucky by investigating dairy management practices and the environmental benefits of hemp production. As dairy prices have been on the decline and dairy co-ops have tightened their restrictions on somatic cell count (SCC) levels, dairy farmers and farm managers must decide the best course of action for maintaining milk quality in order to maintain their contract and profitability. Maintenance decisions as well as factors like sanitation and animal living conditions can all contribute to bulk tank SCC and depending on the type of incentives or penalties instituted by the co-op they can have an impact on net farm income. The objective of the dairy study is to determine which dairy management practices have the largest impact on SCC levels. Industrial hemp is produced worldwide. Historically, the major producers of hemp have been China, Europe, and Russia. In 2014, the passage of the Farm Bill opened the door to the production of Industrial hemp through the development of state pilot programs. Then the 2018 Farm Bill removed industrial hemp from the Scheduled Drug list. This has further expanded the opportunities and excitement for this crop. The plant’s versatility and the variety of products that can be made from it are coming to light. Sustainability is one of the key attributes touted concerning industrial hemp. Specifically, in the state of Kentucky, it is expected to be a replacement for tobacco and other traditional crops. However, how does the crop compare to tobacco production in terms of sustainability? The objective of the hemp study is to develop a life cycle analysis on the planting and harvesting of hemp and compare its impacts to more traditional crops.

This work investigates the fabrication, characterization and testing of Opuntia ficus-indica mucilage nanofibers to be utilized in water filtration systems. These mucilage nanofibers are formed using different polymers through a process called electrospinning. The polymers used to promote the formation of nanofibers are poly vinyl alcohol (PVA) and polystyrene (PS). The mucilage is a jelly like substance extracted from the pads of the cactus plant. It is a mixture of proteins, complex polysaccharides and monosaccharaides. It is an inexpensive, non-toxic, biodegradable and biocompatible material which is present in abundance. The mucilage extracted from the pads is mixed with acetic acid to form the mucilage solution. The mucilage solution is then mixed by volume with co-spinning polymers, PVA and PS. PVA is a synthetic polymer that is water-soluble, and this work considers two types of PVA differentiated based upon molecular weight, such as low molecular weight PVA and high molecular weight PVA. Polystyrene is a synthetic polymer extracted from a monomer styrene, and it is inexpensive, biodegradable, and abundant. The polystyrene, in its solid form, is further decomposed using a solvent called D-Limonene. D-Limonene is a biodegradable, non-toxic solvent formed from the citrus extract of orange peelings. The PVA and PS solutions are mixed in several different volume ratios with the mucilage solutions. These solutions were electrospun and consistent nanofibers were obtained using the low molecular weight PVA solutions and the polystyrene solutions. The fibers and polymeric solutions were characterized by scanning electron microscopy (SEM), contact angle measurements, viscosity, and FTIR. Resulting mucilage nanofiber membranes were characterized by atomic fluorescence spectrometry (AFS) filtration testing. In addition, a life cycle analysis using the SimaPro software was performed to understand the environmental impact of solutions used to fabricate the mucilage nanofiber membranes. Characterization results confirm the formation of PVA:mucilage and PS:mucilage nanofibers. Filtration testing of the nanofiber membranes indicates better performance with membranes formed by PS: mucilage solutions as compared to PVA: Mucilage solutions. Overall, this work has shown that natural materials, such as cactus mucilage, can be synthesized with polymeric solutions to form environmentally friendly water filters.