O flúor tem um papel importante na estrutura dentária diminuindo a desmineralização e potencializando a remineralização. É fundamental conhecer o comportamento dos materiais restauradores no que diz respeito a liberação de flúor, não só em água deionizada, mas também em condições de desafio ácido. O objetivo desse trabalho foi avaliar a liberação de flúor de 6 materiais restauradores, em dois meios de imersão, água deionizada e sistema de ciclagem de pH entre, por 15 dias. Os materiais utilizados foram o Vitremer(VIT), o Dyract (DYR), o Ariston (A), o Tetric Ceram (TET), o Definite (DEF) e o Z 100. Para cada material foram confeccionados 16 corpos-de-prova na forma de discos (11 mm de diâmetro e 1,5 mm de espessura), os quais foram armazenados individualmente em 4 ml de cada solução, sendo 8 para cada meio de imersão. Na ciclagem de pH, o material era mantido 6 horas na solução desmineralizante e 18 horas na solução remineralizante. As soluções foram trocadas diariamente e os espécimes armazenados durante 15 dias. A liberação de flúor foi medida em 0,5 ml de solução adicionada a igual volume de TISAB II. Para os materiais Z 100, DEF e TET em água deionizada, a liberação de flúor foi obtida pela técnica da difusão facilitada por HMDS, nos períodos de 1, 7 e 15 dias. A análise da concentração de flúor foi medida por eletrodo específico para flúor, acoplado ao aparelho analisador de pH/F¯. Todos os materiais liberaram mais flúor no sistema de ciclagem de pH, exceto o Ariston. O pico de liberação foi maior no 1º dia, declinando do 2º dia em diante, e alcançando níveis baixos e constantes a partir do 7 º ao 15º dia. Os resultados foram submetidos a análise de variância a 3 critérios e ao teste de Tukey (p<0,05), para as comparações múltiplas. A liberação total no período do experimento em ordem decrescente, em água deionizada, em µgF¯/mm2 , foi A (3,6603), VIT (1,7529), DYR (0,2481), TET (0,0140), DEF (0,0086) e Z 100 (0,0020) e em ciclagem de pH, VIT (3,4366), A (2,1422), DYR (1,0691), TET (0,0297), Z 100 (0,0067) e DEF (0,0063). Assim concluímos que os materiais apresentaram padrão semelhante de liberação de flúor, exceto o Ariston, que mostrou comportamento estável e liberação constante durante os 15 dias, nos dois meios de imersão. Na ciclagem de pH, houve uma tendência de liberação de flúor de todos os materiais. / The fluoride has an important function in the dental structure reducing the desmineralization and increase the remineralization. It is fundamental to know the behavior of the restoratives materials relative the fluoride release, not only in deionized water, but also in conditions of acid challenge. The aim of the present study was to determine the fluoride release from 6 restoratives materials, in two storage solutions: deionized water and pH-cycling system solution over 15 days. 16 discs (11 mm diameter and 1,5 mm thickness) of materials, Vitremer (VIT), Dyract (DYR), Ariston pHc (A), Tetric Ceram (TET), Definite (DEF) and Z 100 were prepared and suspended individually in 4 mL of each solution, in number of 8 for each medium which was changed every 24 hours. In the pH-cycling system the specimens were immersed 6 hours in the demineralizing solution (pH 4,3) and 18 hours in remineralizing solution (pH 7,0). The solutions were changed daily during a period of 15 days. The fluoride release was determined after buffering the solutions with an equal volume of TISAB II. For the materials Z 100, DEF, TET in deionized water, the fluoride concentration was obtained by the microdiffusion method (Taves) in the periods of 1, 7 and 15 days. The analysis of the fluoride ion concentration in the solutions was measured using a fluoride ion sensitive electrode and a potent iometer ion-analyzer pH/F¯. The results of fluoride concentration in solutions eretransformed into released fluoride amount per material area (µgF¯/mm 2 ). All the materials released more fluoride in the pH-cycling system, exception Ariston. The release peak was larger in the 1st day, dropped at the 2nd and then remained constant from 7º to 15th day. The Ariston showed stable behavior, with small variations along the experiment. The results were submited to statistical analysis by ANOVA and the diferences between the treatments by the Tukey test (p<0,05). In deionized water, fluoride release in mgF¯/mm 2 was A(3,6603), VIT(1,7529), DYR (0,2481), TET (0,0140), DEF (0,0086) and Z 100 (0,0020) and, in pH-cycling system VIT (3,4366), A (2,1422), DYR (1,0691), TET (0,0297), Z 100 (0,0067) and DEF (0,0063), during the whole experimental period.

Certain measures of aerobic power have been shown to have a high relationship with endurance performance. Critical power (CP) has also been shown to be well correlated to endurance performance, but few studies have evaluated its use in a competitive scenario. In this study, cardiorespiratory-metabolic measures were evaluated in 13 highly trained cyclists to determine their relationship to performance in a 17 km time trial. Critical power, determined from the nonlinear power-time model, was also evaluated to determine its relationship to performance in a 17 km time trial. Results indicate that the traditional indicators of V02max and ventilatory anaerobic threshold were well correlated to TT performance (r=-0.86, r=-0.79, respectively). The principal finding from this study was that performance in a bicycle time trial is related to CP at least as well as to cardiorespirator-ymetabolic measures. In fact, the results fromthis study suggest that the relationship between performance and CP is stronger (r=-0.89). Use of the critical power concept is attractive because testing requires only a cycle ergometer and a stopwatch to estimate a parameter of aerobic fitness.

O presente estudo objetiva avaliar os mecanismos de transferência de carbono entre os compartimentos atmosfera, vegetação, solo e igarapé em uma microbacia da Amazônia Ocidental. Dois igarapés drenandos, respectivamente, 2927 e 66,73 ha de floresta de terra firme, foram monitorados durante um ano. A área de estudo se encontra na zona de amortecimento de uma Unidade de Conservação de Uso Sustentável denominada Floresta Estadual do Amapá (FLOTA/AP), na região central do estado de mesmo nome. Foram coletadas as águas da chuva, da precipitação interna da floresta, do escoamento de água pelo tronco, do escoamento superficial pelo solo, da solução do solo, da água subterrânea e da água do igarapé. Os solos também foram investigados em relação a suas características físico-químicas. Para calcular a entrada e saída de C do sistema, foram determinadas as concentrações do carbono orgânico e inorgânico dissolvido (COD e CID, respectivamente) na água da chuva e do igarapé, em 16 eventos de chuva. As concentrações médias de COD na água da chuva foram de 1,6± 1,52 mg L-1, resultando em um aporte de 11,43 Kg C ha-1 ano-1. Na precipitação interna os valores médios observados foram de 9,1 ± 5,99 mg L-1, o que corresponde a um fluxo de 100,71 Kg C ha-1 ano-1. No escoamento do tronco, os valores médios observados foram de 17,4 ± 8,03 mg L-1 e no escoamento superficial do solo de 14,2 ± 6,4 mg L-1. Nos compartimentos amostrados abaixo do solo, solução do solo e água do lençol, as concentrações de COD foram relativamente mais baixas. A saída de COD pelo igarapé, os fluxos foram de 0,45 Kg C ha-1 ano-1. Em relação às concentrações de CID, o aporte pela água da chuva foi de 3,66 Kg C ha-1 ano-1, passando a 10,10 Kg C ha-1 ano-1 na precipitação interna e com uma saída pelo igarapé de 0,07 Kg C ha-1 ano-1. Os resultados mostram grande variabilidade espaço-temporal e retenção de C pelo sistema, seja na fase orgânica (COD) ou inorgânica (CID), demonstrando a importância destes processos para a compreensão do funcionamento destes ecossistemas. / This study aims to evaluate carbon transfer mechanisms between the atmosphere, vegetation, soil and stream in a microbasin of eastern Amazon. Two streams, draining respectively 2917 and 66.73 ha of \"terra firme\" forests were monitored during one year. The study area is located in a Conservation Unit named Amapá State Forest (FLOTA/AP), in the central region of the Amapá State. We sample rain water, throughfall, stemflow, soil surface flow, soil solution, groundwater and stream water. Physico-chemical characteristics of soils were also evaluated. To calculate inputs and outputs of C in this system, we determined the concentrations of dissolved organic and inorganic carbon (DOC and DIC, respectively) in rain and stream water during 16 rain events. Average concentrations of DOC in rain water were 1.6± 1.52 mg L-1, resulting in an input of 11.43 Kg C ha-1 year-1. Throughfall had average concentrations of 9.1 ± 5.99 mg L-1, which increased inputs to 100.71 Kg C ha-1 year-1. Stemflow had average concentrations of 17.4 ± 8.03 mg L-1 while those of soil surface flow were 14.2 ± 6.4 mg L-1. Bellow ground DOC concentrations were relatively lower. The export of DOC in stream water was 0.45 Kg C ha-1 year-1. In relation to DIC, the input from rain water was 3.66 Kg C ha-1 year-1, increasing to 10.10 Kg C ha-1 year-1 in throughfall and exiting the micro basin through the stream with a flux of 0.07 Kg C ha-1 year-1. The results show large spatiotemporal variations and C retention within the system, either in the organic (DOC) or inorganic (DIC) phases, showing the importance of these processes for the comprehension of the functioning of these ecosystems.

Nitrogen cycling was studied in the hypersaline northern arm of the Great Salt Lake (332,480 mg/l total dissolved solids) using a sediment-water microcosm simulation of that system. The study demonstrated that nitrate, ammonia, and urea were not stimulating to the halophilic bacteria in the microcosm. The bacteria were simulated only indirectly simulated bacterial growth. In addition, no nitrification could be demonstrated during the study. Weekly analyses of water samples from the north arm of the lake itself showed that nitrate and nitrite were absent, leaving ammonia as the only significant form of inorganic nitrogen in the northern lake basin. Other work on lake samples has indicated that no nitrogen fixation occurs in this system. Thus, the absence of nitrogen fixation, nitrification, and denitrification (because of the absence of nitrate) make it apparent that the production and the utilization of ammonia are the only two significant nitrogen cycling pathways operating in this system. The equilibrium established between the conversion of ammonia into organic nitrogen and the production of ammonia from mineralization of this organic matter is then responsible for the conditions which are found in the northern arm of the Great Salt Lake.

Priming exercise, or the ‘warm-up’, is an accepted practice prior to exercise participation, physical training or sporting competition. Traditionally, low intensity exercise has been used prior to both short- and long-duration events in an effort to prepare the athlete, but not fatigue them. Recently, however, a more scientific approach to priming exercise has been considered important, with some research suggesting that a high intensity intermittent priming strategy may be optimal. However, given the paucity of performance focussed ‘warm-up’ studies, and that existing data regarding high-intensity priming strategies is inconclusive, the aim of this thesis was to determine the effects of three high-intensity intermittent priming strategies on physiological responses and subsequent 3km laboratory time-trial (TT) performance. Ten well-conditioned endurance-trained male cyclists (mean ± SD: age, 28.3 ± 8.4 yr, body mass, 81.8 ± 11.6 kg, stature, 1.8 ± 0.1 m, O2peak, 4.6 ± 0.5 L•min−1) were recruited for this study. After an initial incremental exercise test to exhaustion, participants completed four 3km time trials (TT) on four separate occasions, each preceded by a different priming strategy. These included a ‘self-selected’ (control) condition, and three high-intensity intermittent priming strategies of varying intensity (100% and 150% of the power at O2peak, and all-out) and fixed duration (15 minutes), each in predetermined random order. Five minutes passive rest separated each priming exercise condition from the experimental 3km-TT. Oxygen uptake ( O2) and heart rate (HR) were measured continuously, while blood lactate concentration ([BLa]) and core temperature (TC) were recorded at rest, post-priming exercise, and immediately prior to and following the 3km-TT. In an attempt to provide a mechanistic explanation for changes in performance, O2 kinetic variables were determined from the O2 data. Performance was quantified as a mean power (Wmean) and total time taken to complete the 3km-TT. Mean power output and time taken for each 500m segment of the 3km-TT were also calculated. Results demonstrated that the athletes self-chosen priming condition (378.6 ± 44.0 W) resulted in Wmean that was slightly greater than both the lowest (376.3 ± 44.9 W; 0.7%; p = 0.57) and moderate (373.9 ± 47.8 W; 1.5%, p = 0.30) intensity intermittent priming condition, but significantly greater than the ‘all-out’ intermittent sprint priming condition (357.4 ± 44.5 W; 5.8%, p = 0.0033). Similar differences were observed for time. While differences existed in the O2 deficit (however, mainly non-significant), these differences did not provide clear explanations for the differences in performance, with the moderate priming condition displaying a significantly reduced O2 deficit (59.4 ± 15.6 L, p < 0.05), despite the non-significant change in Wmean, compared to the self-chosen priming condition (73.3 ± 18.6 L). Additionally no significant differences were observed in either the time constant or the mean response time of O2. Significant findings with regard to HR, [BLa] and TC were observed, but consistent with O2 kinetic variables, they were not related to, nor explain performance changes. In conclusion, regardless of intensity, different high-intensity intermittent priming exercise did not improve 3km-TT performance more than the control condition (self-chosen). A priming strategy that is overly intense was detrimental to subsequent cycling performance. The observed finding that a self-chosen priming strategy resulted in a comparable performance suggests that athletes are able to self-select (consciously or sub-consciously) a ‘warm-up’ that is of appropriate intensity/duration. Further work utilising the priming strategies from the current study with events of shorter duration is required to further clarify how priming strategies of this nature may affect track cycling performance.

Adaptation to the shortage of oxygen at altitude (hypoxia) promotes physiological changes which could enhance endurance performance. Consequently, altitude training has become a popular practice among competitive endurance athletes. Since its inception, the live-high train-low paradigm (LHTL) has been widely regarded as the most effective approach to altitude training. Over the past decade, brief intermittent simulation of LHTL via the use of hypoxic inhalers and re-breathing devices has gained increased popularity, but the evidence supporting their use is limited and conflicting. The experimental studies in this thesis investigated the response of sea level exercise performance and related physiological measures following adaptation to the usual and a novel protocol of brief intermittent hypoxia. I intended to perform all experimental studies on flat-water kayakers. Therefore, an initial requirement of this thesis was to establish the smallest worthwhile effect in performance for this sport. The final study utilising a meta-analytic approach was conducted to compare the effectiveness of brief intermittent hypoxia to other natural and simulated protocols, and to investigate the topical issue of what physiological responses mediate performance changes following hypoxic exposure. In Study 1, the typical variation in competitive performance of elite flat-water canoeists was investigated using a repeated-measures analysis of race times. For individual flat-water canoeing events, the smallest worthwhile change in performance time was ~0.5%. In two separate experimental studies, adaptation to 60 min per day of brief intermittent hypoxia consisting of alternating 5 min intervals of hypoxia and normoxia for 3 weeks (5 days per week) using a nitrogen filtration device resulted in clear enhancement of endurance performance (~5%) for kayakers (Study 2) and cyclists (Study 3). Clear enhancements in repeat sprint performance were observed for kayaking only. The physiological mechanisms underlying performance changes were unclear. Modification of the hypoxic and normoxic intervals (Study 3) did not result in any clear alterations in performance or physiological mechanisms. The meta-analysis (Study 4) revealed clear enhancements in endurance power output of 1-3% in sub-elites following adaptation to hypoxia with the natural altitude protocols, and with two of the artificial-altitude protocols (LHTL-long and LHTL-brief-intermittent). In elite athletes the enhancements tended to be smaller and were clear only for the natural protocols. These enhancements could be mediated by VO2max, although other mechanisms may be possible.

Field experiments were conducted on an Alfisol in the semi-arid tropics of northern Australia to investigate nitrogen (N) cycling in the leucaena (Leucaena leucocephala) alley cropping system. This is a farming system in which maize (Zea mays L.) is grown in alleys formed by leucaena hedgerows spaced 4.5 metres apart. Mineralization of N from Ieucaena (prunings) and maize residues was studied under field conditions. Response of maize growth to addition of N fertilizer and plant residues was evaluated both in field plot and microplot experiments. The fate of fertilizer N and leucaena N was examined over four consecutive seasons. The decomposition (loss of mass) of dry, cut 15N-labelled leucaena residues differed from that of intact fresh leucaena prunings in the first cropping season although no difference was detected after one year. At the end of one cropping season, 3 months after application, 58-72% of 15N-labelled leucaena had decomposed compared to only 34-36% of fresh leucaena prunings. Similar trends occurred at 20 and 52 days after application. The extent of decomposition of fresh leucaena prunings (28-33%) was similar at two loading rates (2.4 and 4.7 t DM ha -1) by 3 months after addition. About 72% of young 15N labelled maize residues was decomposed by 3 months after addition in the presence of fresh leucaena prunings. Decomposition of 15N-labelled leucaena residues and unlabelled fresh prunings was 91% and 88% respectively 14 months after addition. After 2 years the corresponding values were 96% and 94%. When N content of the recovered residues was taken into account, the values were 95% and 94% after 14 months, and the same (97%) after 2 years. Maize yield and N uptake were significantly increased following addition of either unlabelled fresh leucaena residues or 15N-labelled thy Ieucaena residues. Application of N ferilizer produced a thither increase in the presence of the residues. The maize yield and N uptake with the 15N-labelled leucaena were not different from those with the unlabelled residues. There was a significant positive interaction between N fertilizer and leucaena prunings which increased maize production. Addition of maize residues decreased the yield and N uptake of maize compared with that obtained in the presence of N fertilizer at 40 kg N ha~1 and leucaena residues (2.4 t DM ha-1). There was a marked residual benefit of N fertilizer applied in the first season at 36 kgN hat in the presence of leucaena prunings on the second maize crop yield and N uptake, but not on the third crop. However, a significant residual benefit of leucaena prunings added in the first season was found in DM yield and N uptake of the second and third maize crop. The short-term fate of 15N applied in plant residues was examined during two separate cropping seasons. By 20 days after application of separate 15N-labelled leucaena leaves, stems and petioles, 3-9% of the added 15N could be found in maize plants, 33-49% was in surface residues, 36-48% in the 2 m soil proffle and 0.3-22% unaccounted for. In a separate experiment when leucaena components were not separated, 5% of 15N applied in leucaena residues was taken up by maize 52 days after addition, 45% was in residues, 25% was in soil and 25% was unaccounted for. Jn another experiment, maize recovered 6% of added leucaena 15N after 2 months, 39% remained in residues, 28% was in soil and 27% was not recovered. Incorporation of 15N-labelled leucaena residues in the soil did not increase recoveiy of leucaena 15N by maize compared with placement of the residues on the soil surface. By the end of one cropping season (3 months after application), 9% of added 15N was recovered by maize from 15N-labelled leucaena. There was a similar 15N recoveiy from 15N-labelled maize residues applied as mulch at 1.7 t DM ha1 together with unlabelled leucaena prunings at 2.4 t DM ha ~. In both cases, 30-32% of added 15N was detected in soil, 28% in residues, and 31-34% apparently lost. The short-term fate of fertilizer 15N was different from that of 15N added in plant residues. In a 52-day experiment, maize recovered 65-79% of fertilizer 15N applied at low rates (6.1 and 12.2 kg N ha -1) in the presence of leucaena prunings, 21-34% was present in soil, and less than 1% was not recovered. By 2 months after application, recoveiy of fertilizer 15N by maize was 41% from N fertilizer added at 80 kg N ha -1, 35% from N fertilizer at 40 kg N ha -1 in the presence of leucaena prunings, and 24% from N fertilizer at 40 kg N ha -1 in the presence of maize residues and leucaena prunings. The corresponding deficits (unaccounted-for 15N) were 37%, 38% and 47% respectively. A small but significant amount of the fertilizer 15N was present in the unlabelled leucaena residues (3%) and in the mixture of unlabelled leucaena and maize residues (7%) present on the soil surface. However, application of the plant residues did not affect recoveiy of the fertilizer 15N in soil (21-24%). When N fertilizer was applied at 40 kg N hi1 in the presence of leucaena prunings, 43% of fertilizer 15N was recovered by maize at the end of cropping season, 20% in soil, 2% in residues, and 35% unaccounted for. The long-term fate of fertilizer 15N was compared with that of leucaena 15N in an experiment over four cropping seasons. In the first season, maize tops recovered 50% of the fertilizer 15N but only 4% of the leucaena 15N. In the second, third and fourth seasons, maize (tops + roots) recovered 0.7%, 0.4% and 0.3% of the initial fertilizer 15N compared with 2.6%, 1.8% and 1.4% of the initial leucaena 15N. In the second, third and fourth seasons, recovery of the initial fertilizer 15N (12-14%) in soil was much lower than that of the initial leucaena 15N (38-40%). There was no further loss of the fertilizer 15N after the first season. However, the cumulative 15N deficit for the leucaena 1N in the first two seasons was 50%--thissuggested an additional loss of 23% since the end of the first season. There was no further loss of 15N from either residual fertilizer 15N or residual leucaena 15N in the third and fourth seasons. In conclusion, application of leucaena prunings could substantially increase maize yield and N uptake although some supplementary N fertilizer may be required to achieve maximum crop yield. Maize recovered only a small amount of added leucaena N in the first year. Most of the leucaena residue N was present in the soil and remaining residues after one season. This residue N would be gradually available for plant uptake by subsequent crops. Of course, annual additions of leucaena prunings would appreciably increase the pool of available N over time. Thus, application of leucaena prunings could substantially improve soil fertility in the long term.

Cross-country (XC) mountain bike (MTB) riding is a new cycling discipline and research examining the physiological demands of MTB racing is limited. The purpose of this study was to comprehensively measure physiological characteristics, to identify the performance demands of XC and time trial (TT) MTB racing and to simulate a field MTB race in the laboratory to measure the physiological responses associated with racing. Twelve male and four female elite MTB cyclists volunteered to take part in this study. Subjects completed maximal aerobic power and, anaerobic power and capacity tests. MTB race data was collected during TT and XC competitions with SRM MTB power cranks fitted to the subjects MTB. Five male MTB cyclists (V . O2max 72.0 +/- 4.6 ml/kg/min-1, maximum power output (MPO) 5.40 +/- 0.30 W/kg-1, maximum heart rate (HRmax) 189 +/- 7 bpm) performed two laps of a MTB course in the field using their race bikes with MTB SRM power cranks fitted. A laboratory MTB race simulation was performed using a wind braked ergometer. Cyclists attempted to match the average and peak power output (W/kg-1) achieved in the field trial in the laboratory. Power output (PO), heart rate (HR) and cadence (revolutions per minute, rpm) were measured during field and laboratory trials, while oxygen uptake (V . O2) was determined only during the laboratory simulation. Results showed TT MTB racing is significantly shorter in duration and distance than XC racing and significantly higher for power output and heart rate, with more time spent above anaerobic threshold (16.0 +/- 2.4 and 22.8 +/- 4.3% time) and MPO (38.4 +/- 5.2 and 26.5 +/- 9.4% time) than XC racing (p<0.05). Mean power output and heart rate between the field and laboratory trials were similar (4.18 +/- 0.55 and 4.17 +/- 0.15 W/kg-1 respectively, 175 +/- 9 and 170 +/- 8 bpm). Time spent below 2 W/kg-1 and above 6 W/kg-1 for the field and laboratory trials accounted for ~32% and ~30% of the total time, respectively. During field and laboratory trials, cyclists utilised 77.8 and 77.3% of MPO, 93 and 90% of HRmax, respectively. There was a significant difference between mean cadence in the field and laboratory trials (60.3 +/- 9.1 and 75.2 +/- 7.0 rpm, respectively, p<0.05). The cadence band of 60-69 rpm showed a significant difference between the time spent in that band from the field (14.6%) to the laboratory (4.6%). The time spent above a cadence of 80 rpm in the field was 29.8% compared to the laboratory at 62.0% of the time. Mean and peak V . O2 for the simulation was 57.5 +/- 3.3 and 69.3 +/- 4.4 ml/kg-1/min-1 respectively, with cyclists sustaining an average of ~80% V . O2max. In summary, MTB competition requires multiple short-high intensity efforts and places high demands on both the aerobic and anaerobic energy systems. The power output and heart rate responses to a MTB field race are similar when simulated in the laboratory, although in the laboratory higher cadences are selected for the higher power outputs than the field.

PowerCranks(tm) are claimed to increase economy of motion and cycling efficiency by reducing the muscular recruitment patterns that contribute to the resistive forces occurring during the recovery phase of the pedal stroke. However, scientific research examining the efficacy of training with PowerCranks(tm) is lacking. Therefore, the purpose of this study was to determine if five weeks of training with PowerCranks(tm) improves economy of motion (EOM), gross efficiency (GE), oxygen uptake (V.O2) and muscle activation patterns in trained cyclists. Sixteen trained cyclists were matched and paired into either a PowerCranks(tm) (PC) or Normal Cranks (NC) training group. Prior to training, all subjects completed a graded exercise test (GXT) using normal bicycle cranks. Additionally, on a separate day the PC group performed a modified GXT using PowerCranks? and cycled only until the end of the 200W stage (PCT). During the GXT and PCT, FeO2, FeCO2 and V.E were measured to determine EOM, GE and V.O2max. Integrated electromyography (iEMG) was also used to examine selected muscular activation patterns. Subjects then repeated the tests following the completion of training on their assigned cranks.

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