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Cytokine production by cultured bovine mammary epithelial cells (MAC-T) upon stimulation with lipopolysaccharideChan-Tang, Hoi-Sing. January 1998 (has links)
Cytokines are known to be involved in mastitis, yet their production by bovine epithelial cells has so far not been reported. In this research a bovine mammary alveolar cell line (MAC-T) was used as a simplified model for mammary glands. Cultured MAC-T cells were stimulated with 1, 10 and 20 mug/ml of a bacterial cell wall component (lipopolysaccharide) to verify the production of epithelially derived bovine cytokines. Cytokine mRNA production was assessed by reverse transcriptase polymerase chain reaction (RT-PCR) using RNA samples isolated during the first 24 hrs of lipopolysaccharide stimulation. Interleukin-1alpha/beta, interleukin-6 and interleukin-8 mRNA production seemed to be time and dose dependent. Restriction enzyme analysis of PCR products confirmed the identity of the cytokines. Enzyme linked immunosorbent assays for interleukin-1 and interleukin-8 showed that the respective mRNA's were translated into proteins. Interleukin-8 protein was detectable 6 hrs after lipopolysaccharide stimulation; maximal levels of approximately 55 pg/ml were reached at 48 hrs post stimulation. Interleukin-1 was detected 1hr after stimulation; concentrations peaked between 500 and 600 pg/ml at 2, 5 and 12 hrs for 20, 10 and 1 mug/ml of lipopolysaccharide respectively. The amount of protein produced by the MAC-T cell line was relatively low and required high concentrations of lipopolysaccharide. Nevertheless, this model demonstrated a time and dose dependent cytokine production. These results suggest that bovine epithelial cells could be a source of cytokine production during mammary infection.
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Activating point mutations in the common ?gb?s[beta]-subunit of the human GM-CSF, IL-3 and IL-5 receptors : implications for receptor function and role in disease / by Brendan John Jenkins.Jenkins, Brendan John January 1998 (has links)
Includes bibliographical references (17 leaves) / vii, 113, [89] leaves, [32] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Generates and exploits activating point mutations in the common ?gb?s subunit (h?gb?sc) of the human GM-CSF, IL-3 and IL-5 receptors. Elucidates the mechanisms by which the isolated mutations confer constitutive activity on h?gb?sc. Identifies the putative all-specific signalling molecules and provides a map of the locations of activating point mutations in h?gb?sc, / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 1998
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Activating point mutations in the common ?gb?s[beta]-subunit of the human GM-CSF, IL-3 and IL-5 receptors : implications for receptor function and role in disease / by Brendan John Jenkins.Jenkins, Brendan John January 1998 (has links)
Includes bibliographical references (17 leaves) / vii, 113, [89] leaves, [32] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Generates and exploits activating point mutations in the common ?gb?s subunit (h?gb?sc) of the human GM-CSF, IL-3 and IL-5 receptors. Elucidates the mechanisms by which the isolated mutations confer constitutive activity on h?gb?sc. Identifies the putative all-specific signalling molecules and provides a map of the locations of activating point mutations in h?gb?sc, / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 1998
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Innate immune responses of the mouse and jird to filarial parasites and emulsan : a putative adjuvant /Panilaitis, Bruce J. B. January 2001 (has links)
Thesis (Ph.D.)--Tufts University, 2001. / Adviser: Juliet A. Fuhrman. Submitted to the Dept. of Biology. Includes bibliographical references. Access restricted to members of the Tufts University community. Also available via the World Wide Web;
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Human truncated thioredoxin (Trx80) as a novel mitogenic cytokine for white blood cells /Pekkari, Klas, January 2001 (has links)
Diss. (sammanfattning) Stockholm : Karolinska institutet, 2001. / Härtill 5 uppsatser.
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Activating point mutations in the common?gb?s[beta]-subunit of the human GM-CSF, IL-3 and IL-5 receptors : implications for receptor function and role in disease /Jenkins, Brendan John. January 1998 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Medicine, 1998. / Includes bibliographical references (17 leaves ).
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Effector CD4Ê T lymphocytes in the prodrome of polyarthritis /Brasted, Melissa. January 2001 (has links) (PDF)
Thesis (Ph.D.) -- University of Adelaide, Dept. of Molecular Biosciences, 2002. / "October 2001" Amendments (4 leaves) inserted inside back cover. Includes bibliographical references (leaves 215-266).
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Application of proximity ligation for detection of proteins, biomolecular interactions, and single copies of pathogens /Gustafsdottir, Sigrun Margret, January 2006 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 4 uppsatser.
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Synthesis of cytokines and growth factors during bacterial peritonitis complicating peritoneal dialysis in vivo and in vitro studies /Wan, Cheuk-chun. January 2008 (has links)
Thesis (M. Res.(Med.))--University of Hong Kong, 2008. / Includes bibliographical references (p. 106-130)
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Do carbohydrates increase the magnitude of the inflammatory responseDepner, Chris Michael. January 2008 (has links) (PDF)
Thesis (MS)--Montana State University--Bozeman, 2008. / Typescript. Chairperson, Graduate Committee: Mary P. Miles. Includes bibliographical references (leaves 64-67).
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