Made available in DSpace on 2016-04-28T20:17:24Z (GMT). No. of bitstreams: 1 2006-Marcelo Carvalho Gomes.pdf: 324564 bytes, checksum: 3942a2feee43fca4762eb4590c899bfc (MD5) Previous issue date: 2006-03-31 / The present work was carried out in three different goat raisings and 27 females and 2 males were selected for identifying the present microbiota in the preputial and vaginal cavity of goats under three estrous cycle phases: In Februrary, 2005 in the pro-estrum, in May, 2005 in the post-copula and in October, 2005 in the post-partum. The samples were collected by swabs as well as vaginal and preputial washings for studying microbiota and by gynecological brushes for the females esfoliative cytological study. The two greatest bacteria genera prevalence were: Micrococcus spp (89,65 %) following by Bacillus spp. (29,90 %). Filamentous fungi as Cladosporium spp. (26,39 %) and Aspergillus spp. (11,46 %) were the most isolated. On the bacteria cytological test about 76 % of the investigations have been in accordance with the microscopic finding and bacteria isolation. In 89% appraised animal some yeasts presence on sheets was observed in spite of there was isolation just in 7,4 % of the animals. There was similarity between hifas observation and filamentous fungi isolation, in regarding to 53 % of hifas presence investigations. The bacterial and fungical microbiota found on this survey showed the goats vaginal and preputial microbiota diversity and pointed out to the fact that it might be carried during copulation. The number of isolated bacteria species in the post-copulation phase was upper, as well as for yeasts. The vaginal cytology performed significative association in relation to the bacterial isolation, observed by cocos and bacilli presence on the sheets with positive growth. Bacteria potentially pathogenic as Pseudomonas alcalinigenes, P. aeruginosa, and Corynebacterium spp. as well as opportunist fungi as Aspergillus flavus in healthy goats vaginal cavity were found. / O presente trabalho foi desenvolvido em tr?s criat?rios caprinos distintos, de onde tomamos 27 f?meas e 2 machos a fim de identificar a microbiota presente no prep?cio de bodes e na cavidade vaginal de cabras em tr?s fases do ciclo estral: fevereiro de 2005 no proestro, maio de 2005 ap?s a c?pula e outubro de 2005 ap?s o parto. As amostras foram coletadas atrav?s de swab, lavado vaginal e prepucial para o estudo da microbiota e com escovas ginecol?gicas para o estudo colpocitol?gico das f?meas. Os dois g?neros de bact?rias de maior preval?ncia foram os seguintes: Micrococcus spp. (89,65 %) e Bacillus spp. (29,90 %). Os dois g?neros de fungos de maior preval?ncia foram Cladosporium spp. (26,39 %) e Aspergillus spp. (11,46 %). No estudo colpocitol?gico para bact?rias, em 76 % das investiga??es houve concord?ncia entre os achados microsc?picos e isolamento bacteriano. Em 89 % dos animais avaliados foi observada a presen?a de leveduras nas l?minas, mas houve isolamento em apenas 7,4 % dos animais. Em 53 % das investiga??es da presen?a de hifas houve concord?ncia entre a observa??o de hifas e o isolamento de fungos filamentosos. A microbiota f?ngica e bacteriana encontrada no presente trabalho evidenciou a diversidade da microbiota vaginal e prepucial de caprinos e aponta para o fato de que podem ser carreados durante a c?pula. Observou-se que o n?mero de esp?cies isoladas de bact?rias ? superior na fase de p?s-c?pula enquanto que em fungos observamos este aumento no p?s-parto; O resultado da citologia vaginal teve correla??o com o isolamento bacteriano, observada pela presen?a de cocos e bacilos nas l?minas com crescimento bacteriano positivo; Bact?rias potencialmente patog?nicas como Pseudomonas alcaligenes, P. aeruginosa e Corynebacterium spp. foram encontrados na cavidade vaginal de cabras h?gidas, assim como fungos oportunistas como Aspergillus flavus.