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Back pain is an important health and economic problem affecting a significant part of our population. It is of interest to both medical and behavioral professionals concerned with the complex role of the social and psychological factors in the etiology of somatic ailments. Although there has been much written about back injuries in military and industrial settings, little is known about the epidemiological patterns in a general population (Nagi et al., 1973). The objective of this study is to find: a) the major factors connected to back pain, b) whether the general work-stress index is related to back pain, where the general work-stress index is the sum of job stressors including psychological demands, job insecurity, physical exertion, decision latitude and the social support at work, and c) the relationship especially amongst back pain, activity restriction, age, job satisfaction and income. The National Population Health Survey (NPHS) database is used in this project. Some statistical techniques such as logistic regression and log-linear models are used for data analysis. In this project all explanatory variables in logistic regression models are treated as continuous variables; all variables when used in log-linear models are treated as categorical data. Results are compared between these different methods. They are in close agreement with each other. We conclude that age has very high impact on back pain with significance level being lower than 1 %; activity restriction also has a strong relationship with back pain; chronic stress, childhood and adult stressors all have high association with back pain; job stressor and recent life bad events are related fairly to back pain at significant level 5%; and income and job satisfaction do not have direct impact on back pain. Although there is not much that can be done to change the normal aging process of the spinal column, some of the predictors identified such as job stressors are amenable to change. / Thesis / Master of Science (MS)

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Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalance between reactive oxygen species or other oxidants and the capacity of antioxidant defense systems to remove them. These oxidants cause wide-ranging damage to macromolecules, including proteins, lipids, DNA and carbohydrates. Oxidative stress is an important pathophysiologic component of a number of diseases, such as Alzheimer's disease, diabetes and certain cancers. Cells contain effective defense mechanisms to respond to oxidative stress. Despite much accumulated knowledge about these responses, their kinetics, especially the kinetics of early responses is still not clearly understood. The Yap1 transcription factor is crucial for the normal response to a variety of stress conditions including oxidative stress. Previous studies on Yap1 regulation started to measure gene expression profile at least 20 minutes after the induction of oxidative stress. Genes and pathways regulated by Yap1 in early oxidative stress response (within 20 minutes) were not identified in these studies. Here we study the kinetics of early oxidative stress response induced by the cumene hydroperoxide (CHP) in Saccharomyces cerevisiae wild type and yap1 mutant. Gene expression profiles after exposure to CHP were obtained in controlled conditions using Affymetrix Yeast Genome S98 arrays. The oxidative stress response was measured at 8 time points along 120 minutes after the addition of CHP, with the earliest time point at 3 minute after the exposure. Statistical analysis methods, including ANOVA, k-means clustering analysis, and pathway analysis were used to analyze the data. The results from this study provide a dynamic resolution of the oxidative stress responses in S. cerevisiae, and contribute to a richer understanding of the antioxidant defense systems. It also provides a global view of the roles that Yap1 plays under normal and oxidative stress conditions. / Ph. D.

The Goodwin Hall building on the Virginia Tech campus is the most instrumented building for vibration monitoring. It houses 225 hard-wired accelerometers which record vibrations arising due to internal as well as external activities. The recorded vibration data can be used to develop real-time applications for monitoring the health of the building or detecting human activity in the building. However, the lack of infrastructure to handle the massive scale of the data, and the steep learning curve of the tools required to store and process the data, are major deterrents for the researchers to perform their experiments. Additionally, researchers want to explore the data to determine the type of experiments they can perform. This work tries to solve these problems by providing a system to store and process the data using existing big data technologies. The system simplifies the process of big data analysis by supporting code re-usability and multiple programming languages. The effectiveness of the system was demonstrated by four case studies. Additionally, three visualizations were developed to help researchers in the initial data exploration. / Master of Science / The Goodwin Hall building on the Virginia Tech campus is an example of a ‘smart building.’ It uses sensors to record the response of the building to various internal and external activities. The recorded data can be used by algorithms to facilitate understanding of the properties of the building or to detect human activity. Accordingly, researchers in the Virginia Tech Smart Infrastructure Lab (VTSIL) run experiments using a part of the complete data. Ideally, they want to run their experiments continuously as new data is collected. However, the massive scale of the data makes it difficult to process new data as soon as it arrives, and to make it available immediately to the researchers. The technologies that can handle data at this scale have a steep learning curve. Starting to use them requires much time and effort. This project involved building a system to handle these challenges so that researchers can focus on their core area of research. The system provides visualizations depicting various properties of the data to help researchers explore that data before running an experiment. The effectiveness of this work was demonstrated using four case studies. These case studies used the actual experiments conducted by VTSIL researchers in the past. The first three case studies help in understanding the properties of the building whereas the final case study deals with detecting and locating human footsteps, on one of the floors, in real-time.

Andromeda is an interactive visualization tool that projects high-dimensional data into a scatterplot-like visualization using Weighted Multidimensional Scaling (WMDS). The visualization can be explored through surface-level interaction (viewing data values), parametric interaction (altering underlying parameterizations), and observation-level interaction (directly interacting with projected points). This thesis presents analyses on the collaborative utility of Andromeda in a middle school class and the insights college-level students generate when using Andromeda. The first study discusses how a middle school class collaboratively used Andromeda to explore and compare their engineering designs. The students analyzed their designs, represented as high-dimensional data, as a class. This study shows promise for introducing collaborative data analysis to middle school students in conjunction with other technical concepts such as the engineering design process. Participants in the study on college-level students were given a version of Andromeda, with access to different interactions, and were asked to generate insights on a dataset. By applying a novel visualization evaluation methodology on students' natural language insights, the results of this study indicate that students use different vocabulary supported by the interactions available to them, but not equally. The implications, as well as limitations, of these two studies are further discussed. / Master of Science / Data is often high-dimensional. A good example of this is a spreadsheet with many columns. Visualizing high-dimensional data is a difficult task because it must capture all information in 2 or 3 dimensions. Andromeda is a tool that can project high-dimensional data into a scatterplot-like visualization. Data points that are considered similar are plotted near each other and vice versa. Users can alter how important certain parts of the data are to the plotting algorithm as well as move points directly to update the display based on the user-specified layout. These interactions within Andromeda allow data analysts to explore high-dimensional data based on their personal sensemaking processes. As high dimensional thinking and exploratory data analysis are being introduced into more classrooms, it is important to understand the ways in which students analyze high-dimensional data. To address this, this thesis presents two studies. The first study discusses how a middle school class used Andromeda for their engineering design assignments. The results indicate that using Andromeda in a collaborative way enriched the students' learning experience. The second study analyzes how college-level students, when given access to different interaction types in Andromeda, generate insights into a dataset. Students use different vocabulary supported by the interactions available to them, but not equally. The implications, as well as limitations, of these two studies are further discussed.

Systems biology is an interdisciplinary field that focuses on elucidating complex biological processes (systems) by investigating the interactions among its components through an iterative cycle composed of data generation, data analysis and mathematical modeling. Our contributions to systems biology revolve around the following two axes: - Data analysis: Two data analysis projects, which were initiated when I was a co-op at GlaxoSmithKline, are discussed in this thesis. First, next generation sequencing data generated for a phase I clinical trial is analyzed to determine the altered microbial community in human gut before and after antibiotic usage (Chapter 2). To our knowledge, there have not been similar comparative studies in humans on the impacts on the gut microbiome of an antibiotic when administered by different modes. Second, publicly available gene expression data is analyzed to investigate human immune response to tuberculosis (TB) infection (Chapter 3). The novel feature of this study is systematic drug repositioning for the prevention, control and treatment of TB using the Connectivity map. - Mathematical modeling: Polynomial dynamical systems, a state- and time- discrete logical modeling framework, is used to model two biological processes. First, a denitrification pathway in Pseudomonas aeruginosa is modeled to shed light on the reason of greenhouse gas nitrous oxide accumulation (Chapter 4). It is the first mathematical model of denitrification that can predict the effect of phosphate on the denitrification performance of this bacterium. Second, an iron homeostasis pathway linked to iron utilization, oxidative stress response and oncogenic pathways is constructed to investigate how normal breast cells become cancerous (Chapter 5). To date, our intracellular model is the only expanded core iron model that can capture a breast cancer phenotype by overexpression and knockout simulations. / Ph. D.