Neste trabalho resultados experimentais inéditos da taxas de perdas para o sistema Cs-K em função da intensidade do laser de aprisionamento foram obtidos. A análise dos resultados foi auxiliada pelo modelo tipo Gallagher-Pritchard que demonstrou possuir uma dependência muito forte com a velocidade de escape. Um estudo complementar ajudou na determinação dos mecanismos causadores de perdas, a catálise óptica, onde o principal resultado foi a obtenção de um resultado teórico que corresponde a observação experimental para o sistema Na-Rb, onde as perdas foram associadas a atuação do estado duplamente excitado. Compreender os mecanismos causadores de perdas pode ajudar na construção de armadilhas magneto-ópticas de grande eficiência, importantes em experimentos de medidas de propriedades atômicas. / We have investigated trap loss rate as a function of trap laser intensity for the Cs-K system. A model based on Gallagher-Pritchard type considerations, allow understand the obtained results. To correctly interpret the data, we have proposed new mechanisms, which can be proven with recent experiment in Na-Rb system.

There are a growing variety of research fields requiring non-contact micro- manipulation. An increasing number of these fields are turning to optical tweezers as a solution, owing to their high spatial and temporal resolution. Optical tweezers have the ability to quantitively exert and measure forces on the piconewton scale, a convenient force scale for soft biological materials, and are hugely versatile due to the wide assortment of beam shaping techniques that can be employed. The work in this thesis can be broadly divided into two main themes: that quantifying the optical trapping forces in shaped beams; and bringing control and simplification of complex systems to non-expert users who may utilise optical tweezers as part of interdisciplinary collaborations. Static beam shaping is used to generate a conically refracted optical trap and the trapping properties are characterised. It is shown that trapping in the lower Raman spot gives full, 3D gradient trapping, while the upper Raman spot allows for particle guiding due to its levitation properties. Particles in the Lloyd/Poggendorff rings experience a lower trap stiffness than particles in the lower Raman spot but benefit from rotational control. Dynamic beam shaping techniques are exploited for the simplification of complex systems through the development and testing of the HoloHands program. This software allows a holographic optical tweezers experiment to be controlled by gestures that are detected by a Microsoft Kinect. Multiple particle manipulation is demonstrated, as well as a calibration of the tweezers system. Application of trapping forces is demonstrated through an examination of integrin – ligand bond strength. Both wild type effector T cells and those with a kindlin-3 binding site mutation similar to that found in neutrophils from Leukocyte Adhesion Deficiency sufferers are investigated. Through the use of back focal plane interferometry, a bond rupture force of (17.9 ± 0.6) pN at a force loading rate of (30 ± 4) pN/s, was measured for single integrins expressed on wild type cells. As expected, a significant drop in rupture force of bonds was found for mutated cells, with a measured rupture force of (10.1 ± 0.9) pN at the same pulling rate. Therefore, kindlin-3 binding to the cytoplasmic tail of the β2-tail directly affects bond strength of single integrin-ligand bonds. An experimental system for studying these cells under more physiologically relevant conditions is also presented. Additionally, a low-cost optical micromanipulation system that makes use of simple microfabricated components coupled to a smartphone camera for imaging is proposed and demonstrated. Through the layering of hanging droplets of polydimethylsiloxane (PDMS) on microscope coverslips, lenses capable of optical trapping are created. Combination of PDMS with Sudan II dye led to the fabrication of long pass filters. An extension of this low-cost system into the life sciences is proposed through the adaptive use of bubble wrap, which allows for the culturing of cells in a chamber compatible with optical trapping.

Neste trabalho reportamos a produção da primeira armadilha magneto-ótica que confina simultaneamente duas espécies atômicas distintas: Sódio e Potássio. Para podermos realizar este aprisionamento, foi necessário vencer algumas dificuldades técnicas que justificam, inclusive, a escolha dos elementos utilizados. Nossa armadilha também foi utilizada para realizar o primeiro estudo de colisões frias entre átomos de espécies diferentes. Experimentalmente, as informações sobre essas colisões são obtidas através da medida da dinâmica de perdas da armadilha de S6dio em presença e ausência de átomos frios de Potássio. Observamos que o efeito de colisões heteronucleares e dez vezes menor do que as homonucleares. Esta diferença já era esperada devido ao menor alcance dos potenciais de interação entre átomos no caso de espécies distintas. Nossos resultados são comparados a uma teoria semi-clássica simples e se encontram em bom acordo com as previsões. Introduzimos uma nova técnica que consiste em mudar repentinamente a intensidade do laser aprisionador e observar a variação do número de átomos aprisionados. Medimos a taxa de perdas por colisão entre átomos de Potássio frios como função da intensidade do laser aprisionador. Essa técnica nos permite alcançar o regime de baixas intensidades, inclusive abaixo da intensidade de saturação, sem as limitações da técnica tradicional. Aplicamos essa técnica ao aprisionamento simultâneo e medimos a taxa de perdas por colisão do sódio na presença e ausência de potássio. Com essa medida somos capazes de estimar a seção e choque entre sódio e potássio ambos no estado fundamental / In this thesis we report the production of the first magneto-optical trap that confines simultaneously two atomic species, sodium and potassium. In order to realize this experiment we had to overcome some technical difficulties that justified our choice of these two elements. This trap was used to study cold collisions between two different species. The information about these collisions is obtained experimentally from the dynamics of the sodium trap loss process in the presence and absence of potassium atoms. We observed that the heteronuclear effect is ten times smaller than the homonuclear one. This difference is explained by the smaller range of the interaction potentials between different species. Our results are compared with the theoretical predictions of the semi-classical theory and show a good agreement with the predictions. We have introduced a new technique which consists of a sudden decrease of the laser intensity, after which we observe the temporal variation in the number of trapped atoms. We have measured the loss rate coefficient between cold potassium atoms as a function of light intensity of the trapping laser. This technique allowed us to reach the very low intensity regime, as low as 30% of the saturation intensity, without compromising the loading process. We applied this technique to the simultaneous trapping and measured the loss rate coefficient between cold sodium atoms in the presence and absence of cold potassium atoms. With this measurement we can estimate the value of the cross section between sodium and potassium in the ground state

Two field experiments determined an effective intertrap distance (ITD) for early detection and delimitation sparse gypsy moth (Lepidoptera: Lymantriidae, Lymantria dispar L.) populations in mountainous terrain. This study found that current Animal and Plant Health Inspection Service trapping guidelines are not sufficient for early detection of small gypsy moth populations in mountainous terrain. Detection trapping in mountainous terrain should have an ITD of not more than 804 m. Delimiting trapping should use a grid design with an ITD of 152 m. A related study determined natural adult male mortality in the climate of the intermountain West, which includes Utah, Nevada, western Wyoming, and southern Idaho. An interaction was found between mortality, temperature, and humidity. During high temperatures, most mortality occurred on the second day. When lower temperatures prevailed, the largest percent mortality occurred on the third or fourth day.

Runoff from road construction and maintenance sites is responsible for erosion and deposition of sediments in the receiving water bodies. In addition to soil particles from erosion, runoff also transports other pollutants such as rubber, toxic metals, automobile fluids, car exhausts (which settle with the rain), pesticides, fertilizers, and other debris. Compost has been used effectively as a valuable soil amendment to aid plant growth. Berms (mounds) of compost placed at the top or bottom of steep slopes can be used to slow the velocity of water and provide additional protection for receiving waters. However, a downside of the application of composted organic material is the potential degradation of runoff water quality. Overloading with nitrogen and phosphorus causes eutrophication, which reduces the suitability of waterways for beneficial uses. A field testing of the berms coupled with a laboratory analysis of the testing water will provide a basis for the impact of the compost berms on the runoff water quality. The study of the impact of compost on the runoff water quality was investigated. The objective of this study was to evaluate the performance of berms made from various materials such as dairy manure compost, yard waste compost and composted bio-solids mixed with wood chips in a ratio of 50:50 on the runoff water quality, as well as, the sediment removal efficiencies. Field tests were performed on the berms to simulate conventional rainfall runoff and the tested water was collected as time-weighted samples and analyzed in the laboratory. Several variables were investigated during this study. Results of this investigation demonstrated that the effectiveness of this application was hampered by the structural instability of the berm. A 100% failure rate was observed in the berms tested. Optimum performance was observed in yard waste compost berms, which introduced the least amount of contaminants into the water. However, some masking effect could be present due to berm failures. In fact, the actual sediment removal by the berms could not be determined. The study of compost filter berms showed some evidence of the existence of first flush effect.

In 1944, the Cooperative Commonwealth Federation (CCF) entered northern Saskatchewan with the goal of utilizing natural resources and restructuring the northern economy through conservation and social policy in order to rehabilitate what they viewed as an impoverished Aboriginal population. This thesis analyzes the affects of government policy on the northwestern Saskatchewan Metis during the mid-twentieth century. Specifically, this study will examine how CCF policy affected the trapping economy and the socio-cultural traditions of the northern Metis. The northwestern Saskatchewan Metis participated in trapping as one of their main sources of income, while facing deflating market prices and government intervention. Through an analysis of archival records that included government documents, government employee and northern Metis correspondence, newspapers, community and government research initiatives and transcribed interviews done by previous projects, this study found that the new government policies were met with resistance by Metis trappers who wished to maintain their traditional trapping practices. Trapping for the Metis, was not only a source of income, it was a livelihood inseparable from their socio-cultural identities and worldview. Therefore, Metis worldview had a direct connection to their acceptance and resistance of CCF policy. More specifically, the northwestern Saskatchewan Metis had a specific “work ethic.” In order to explain Metis reactions to CCF policy Max Weber’s theoretical framework of a “work ethic” derived from The Protestant Ethic and the Spirit of Capitalism was utilized. In this thesis it is posited that the Metis work ethic was based upon the concept of wahkootowin, which placed high value on kinship systems and reciprocity. Wahkootowin encompassed all aspects of northern Metis life including the economy. These cultural values were also juxtaposed with living a “northern style of life,” which involved hard work and survival skills that allowed the Metis to flourish within the northern landscape. In the mid-twentieth century CCF conservation and social policy conflicted with the northwestern Saskatchewan Metis work ethic that was based on the principles of wahkootowin and the northern style of life.

Runoff from road construction and maintenance sites is responsible for erosion and deposition of sediments in the receiving water bodies. In addition to soil particles from erosion, runoff also transports other pollutants such as rubber, toxic metals, automobile fluids, car exhausts (which settle with the rain), pesticides, fertilizers, and other debris. Compost has been used effectively as a valuable soil amendment to aid plant growth. Berms (mounds) of compost placed at the top or bottom of steep slopes can be used to slow the velocity of water and provide additional protection for receiving waters. However, a downside of the application of composted organic material is the potential degradation of runoff water quality. Overloading with nitrogen and phosphorus causes eutrophication, which reduces the suitability of waterways for beneficial uses. A field testing of the berms coupled with a laboratory analysis of the testing water will provide a basis for the impact of the compost berms on the runoff water quality. The study of the impact of compost on the runoff water quality was investigated. The objective of this study was to evaluate the performance of berms made from various materials such as dairy manure compost, yard waste compost and composted bio-solids mixed with wood chips in a ratio of 50:50 on the runoff water quality, as well as, the sediment removal efficiencies. Field tests were performed on the berms to simulate conventional rainfall runoff and the tested water was collected as time-weighted samples and analyzed in the laboratory. Several variables were investigated during this study. Results of this investigation demonstrated that the effectiveness of this application was hampered by the structural instability of the berm. A 100% failure rate was observed in the berms tested. Optimum performance was observed in yard waste compost berms, which introduced the least amount of contaminants into the water. However, some masking effect could be present due to berm failures. In fact, the actual sediment removal by the berms could not be determined. The study of compost filter berms showed some evidence of the existence of first flush effect.

Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / No leaf vii so pagination is misnumbered. Typescript. Vita. Includes bibliographical references. Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 2001. / No leaf vii so pagination is misnumbered. Typescript. Vita. Includes bibliographical references. Also available on the Internet.

Geological carbon dioxide (CO₂) capture and storage in geological formations has the potential to reduce anthropogenic emissions. The viability of technology depends on the long-term security of the geological CO₂ storage. Dissolution of CO₂ into the brine, resulting in stable stratification, has been identified as the key to long-term storage security. The dissolution rate determined by convection in the brine is driven by the increase of brine density with CO₂ saturation. Here we present a new analog laboratory experiment system to characterize convective dissolution in homogeneous porous medium. By understanding the relationship between dissolution and the Rayleigh number in homogeneous porous media, we can evaluate if convective dissolution occurs in the field and, in turn, to estimate the security of geological CO₂ storage fields. The large experimental assembly will allow us to quantify the relationship between convective dynamics and the Rayleigh number of the system, which could be essential to trapping process at Bravo Dome. A series of pictures with high resolution are taken to show the existence and movement of fingers of analog fluid. Also, these pictures are processed, clearly showed the concentration of analog fluid, which is essential to analyze the convective dissolution in detail. We measured the reduction in the convective flux due to hydraulic dispersion effect compared to that in homogeneous media, to determine if convective dissolution is an important trapping process at Bravo Dome. / text