Theses (M.S)--Marshall University, 2007. / Title from document title page. Includes abstract. Document formatted into pages: contains vii, 96 pages. Bibliography: p.87-92.

It is well established that cyclin and cyclin-dependent kinase (CDK) form complex that plays a central role in driving cell cycle progression. The fundamental functions of CDK and cyclin are well conserved across eukaryotes. However, gene families encoding the two type of proteins are significantly expanded in multicellular organisms compared with single-cell species. Despite intensive studies on CDK and its associated cyclin in cultured cell lines, especially in cancer cell lines, the partnership between individual CDKs and cyclins remains elusive especially in vivo. Here I present our preliminary results on establishing the molecular function of a well-conserved cyclin L encoded by cyl-1 in C. elegans. Human cyclin L was demonstrated to form a complex with both CDK11 and CDK12, but its association with the latter remains controversial. Despite a possible function in both transcription and pre-mRNA splicing as suggested by in vitro studies or in yeast, the in vivo function of cyclin L has yet been established in any species. To study cyl-1's function in vivo, we generated multiple strains each expressing a chromosomally integrated single-copy transgenes consisting of CYL-1::GFP flanked by its native regulatory sequences using miniMos technique. The transgene demonstrates ubiquitous expression in nuclei across developmental stages and cell types with few exceptions, including maturing oocytes, in which gene activity is known to be shut down, consistent with its function in transcription and splicing. Co-immunoprecipitation followed by mass spectrometry reveals that CYL-1 interacts with both CDK-11 and CDK-12 along with some other uncharacterized factors. Functional validation of these interactions is underway.

The purpose of the proposed study was to understand the process of career identity development among women immigrants arriving as applicants with dependent status, where career identity refers to a generalized perception of one’s career-related interests and potential in terms of acceptable career roles. Previous research has shown that the majority of dependent applicants under the economic class of immigrants are women and constitute a group of talented persons, possessing the skills to contribute positively to Canada’s economy. However, immigrant women who arrive on a dependent visa to Canada have largely been ignored within immigrant literature. Exploring their career-related experiences upon immigration may assist in understanding the effect of these experiences on their developing career identity. This in turn may lead to useful information in terms of how to tap into their potential and help them realize their career goals, within an evolving process of career development. Adopting a constructivist approach, a qualitative enquiry inspired by a grounded theory methodology was carried out to identify some of the key themes relevant to immigrant women’s career identity development and possible interactions between those themes. Purposive and snowball sampling were used to recruit six participants for the study. Semi-structured interviews with open-ended questions were conducted with each participant. Data were analyzed using Braun and Clarke’s (2012) six-phase method of conducting thematic analysis to generate themes and subthemes. Eight main themes were generated and include: On board the Canadian dream, Coming to terms with “dependent” status, Maintaining equilibrium, Tipping point, Grieving the loss of preferred career trajectory, Taking agency, Redirecting one’s career path, and Emergence of a strengthened career identity. Findings from the study shed light on some of the gender-specific experiences that women immigrants undergo in their career journey, and may provide useful information to career counsellors who play a critical role in helping immigrants navigate through career transitions in a new country.

archives@tulane.edu / Abstract: A search in a research database for “large specific rotation” or anything similar produces few articles. Large specific rotation is not commonly used as an indicator for extraordinary chiroptical response. Alternatively, anisotropy factors obtained from circular dichroism spectra and calculated rotational strengths are more widely used to gauge chiroptical response. To another point, a search for “large chiroptical response” gives few articles that discuss pure organic compounds, and the result list is populated by organometallic clusters, nanostructures, and thin films. A search of the Reaxys database for organic compounds with [α]Ds larger than 1000 revealed that there are about 600, and there are only two that have [α]Ds larger than 10,000.30 We wondered if we could design a compound that would break the record in specific rotation and possess extraordinary chiroptical properties. Guided by time-dependent density functional theory (TD-DFT) calculations, various chiral, polycyclic aromatic compounds (PACs) were chosen as candidates to display extraordinary chiroptical properties, such as high optical rotation, strong circular dichroism, or a high degree of circularly polarized luminescence (CPL). PACs comprise a large class of organic compounds. In addition to synthetic PACs, numerous naturally occurring PACs exist in coal tar and as decomposition products of organic material. Since their pi electrons are delocalized, PACs have interesting and possibly useful electronic properties and a variety of applications. The PACs described in this dissertation, e.g., helical mesobenzanthrones, a cyclophane, are twisted pentacenes are chiral and have interesting optoelectronic properties. TD-DFT was primarily used to predict which compounds had the greatest potential to yield record-breaking specific rotations or other chiroptical properties, and ordinary DFT calculation were used to determine if these compounds had sufficiently high racemization barriers to be resolved at room temperature. With regard to specific rotation, the accuracy of TD-DFT calculations was examined by comparing experimental specific rotations to the calculated values. / 1 / Kelly Jane Dougherty

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During routine measurements of body composition using a 153Gd based dual photon densitometer, it was observed that negative values were being obtained for the body fat fraction in some adults, in children and in small animals. In these three groups, there appears to be a body size dependent error whereby the measured fat fraction becomes increasingly negative as subject size becomes smaller. The fat fraction is derived from relating the measured mass attenuation coefficient of soft tissue to an internal calibration based on the use of water and lard as substitutes for muscle and fat. To investigate whether this procedure for instrument calibration is the cause of the fat fraction errors, soft tissue phantoms which contained known amounts of fat, water and protein were prepared. Over the range of fat fractions used, accurate results were obtained. By using prepared soft tissue and water phantoms it was established that the measured fat fraction incorrectly became progressively smaller as object thickness decreased and incorrectly increased with object thickness. However, accurate measurements were obtained if the equivalent tissue thickness is greater than 9 em and less tnan 16 em of water. Equally reproducible measurements are obtained at all thicknesses investigated. When dual photon measurements of body composition in 13 adolescent females were compared with measurements obtained from skinfold thicknesses or bioimpedance, there was good agreement between techniques but dual photon results demonstrated a broader range of variation with body size. Comparisons between dual photon absorptiometry derived body composition measurements of 52 male athletes with results obtained from under water weighing allowed for derivation of a simple correction factor for the accuracy errors due to body size. / Thesis / Master of Science (MSc)

by Sarah Miu Yin Ip. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 40-44). / Abstract also in Chinese. / Abstract --- p.ii / Acknowledgements --- p.iii / Table of Contents --- p.iv / List of Tables --- p.v / List of Appendix --- p.vi / Introduction --- p.1 / Method --- p.13 / Results --- p.20 / Discussion --- p.33 / References --- p.40 / Appendix --- p.45

Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references.

Based on a natural unification of logic and computation, Martin-Löf’s intuitionistic type theory can be regarded simultaneously as a computationally meaningful higher-order logic system and an expressively typed functional programming language, in which proofs and programs are treated as the same entities. Two modes of programming can then be distinguished: in externalism, we construct a program separately from its correctness proof with respect to a given specification, whereas in internalism, we encode the specification in a sophisticated type such that any program inhabiting the type also encodes a correctness proof, and we can use type information as a guidance on program construction. Internalism is particularly effective in the presence of inductive families, whose design can have a strong influence on program structure. Techniques and mechanisms for facilitating internalist programming are still lacking, however. This dissertation proposes that internalist programming can be facilitated by exploiting an interconnection between internalism and externalism, expressed as isomorphisms between inductive families into which data structure invariants are encoded and their simpler variants paired with predicates expressing those invariants. The interconnection has two directions: one analysing inductive families into simpler variants and predicates, and the other synthesising inductive families from simpler variants and specific predicates. They respectively give rise to two applications, one achieving a modular structure of internalist libraries, and the other bridging internalist programming with relational specifications and program derivation. The datatype-generic mechanisms supporting the applications are based on McBride’s ornaments. Theoretically, the key ornamental constructs — parallel composition of ornaments and relational algebraic ornamentation — are further characterised in terms of lightweight category theory. Most of the results are completely formalised in the Agda programming language.