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This work deals with the study of the possibility of creating an electrochemical biosensor for the detection of specific nucleic acid sequences. The theoretical part describes various types of RNA, with a special focus on miRNAs and possibilities of its detection. It also describes various electrochemical detection approaches, both nucleic acids, and more specifically miRNAs. miRNA is a potential biomarker that can be used for early and non-invasive diagnosis of cancer. The experimental part is aimed at designing, optimizing and preparing a sensitive biosensor for the detection of miRNA-21. A biosensor was prepared to detect the lowest concentration of miRNA-21 in the sample. A linear concentration range for the calibration curve from 1 nM to 1 µM concentration was measured. We measured LOD of 1 fM and by calculation from the regression equation to 3.2 zM and LOQ was 10.8 zM. Subsequently, samples were measured in artificial urine to verify the functioning of the sensor in real conditions. The results showed a minimal effect of the matrix on the determination of the target miRNA.

This master thesis deals with the issues of the eye blink recognition from video. The main task is to analyse algorithms dealing with a detection of persons and make a program that could recognize the eye blink. Analysis of these algorithms and their problems are in the first part of this thesis. In the second part design and properties of my program are described. The realization of the program is based on the method of move detection using the accumulated difference frame, which helps to identify the eye areas. The eye blink detection algorithm tests a match between a tresholded pattern of the eye area taken from the actual frame and the frame before. The resolution whether the eye blink happened or not, is based on the level of the match. The algorithm is designed for watching a sitting man, which is slightly moving. The background can be a little dynamic as well. An average quality video with a moderator and dynamic backround was used as a tested subject.

The diploma thesis dealt with the theme "The use of NIR spectroscopy in the control of goat whey adulteration". The aim of this thesis was to verify the potential of NIR spectroscopy to check of devaluation goat whey with cow whey. In the theoretical part was briefly described the problems of food adulteration, some of the methods used in the control of food with focusing on the near-infrared spectroscopy technique and its application in the dairy industry. Further was described whey and its composition focusing on goat whey, whey processing and the possibilities of using whey in food industry. In the practical part were measured goat and cow whey and their binary mixtures at various concentrations. For the qualitative evaluated of NIR spectra was used discriminant analysis. The results showed that near-infrared spectroscopy that near-infrared spectroscopy is suitable for the authentication of goat whey.

Mastitis is a very serious disease. We distinguish between clinical and subclinical form, contagious and environmental mastitis. Method for the detection are of many. Since somatic cell count, pH, NK assays to detect the help of enzymes. The most important enzymes for the detection of lactate dehydrogenase, Nagasse, lactoferrin, plasmin, alkaline phosphatase and beta-glucuronidase. Dissertation dealt with whether the activity of enzymes, lactate dehydrogenase primarily on the number of somatic cells. This hypothesis was confirmed (p> 0.05) Furthermore addressed the question whether when spinning will decrease the number of somatic cells, w0hich was statistically significant (p <0.05).

This Thesis deals with proposal, implementation and testing of a program for detection of objects to be manipulated and a program for control of robotic arm Melfa RV-2AJ manipulating with these objects. The Theoretical part describes selected algorithms for object detection using a Basler camera and introduces options for controlling the robotic arm Melfa RV-2AJ. The Practical part focuses on development and implementation of a program in the LabVIEW Development Environment for object detection using LabVIEW module NI Vision Acquisition Software and NI Vision Builder for Automated Inspection. Furthermore it describes the development of a program for control of robotic arm Melfa RV-2AJ with use of the LabVIEW module for Mitsubishi robot control called Imaging Lab. The program has been tested by a set of testing tasks and the test results subsequently used for suggestions for functionality improvements.

The aim of this thesis is to introduce the principles of detection and analysis of volatile compounds and sensors for this application. Devise and implement a functional pro-totype electrocal analyzer for evaluating the presence of selected volatile compounds and planttion of essential oils. The analyzer is equipped with a user-friendly operation, including displaying the results of measurements and data recording. The functionality of the device to verify, on a sample substances. At the conclusion of the evaluation results.

This thesis deals with the technological process of brewing beer, describes the raw materials needed for its production, and points out the useful and harmful substances contained in beer as biogenic amines (BA). Furthermore, there are described the issues of biogenic amines in food and primarily in beer, which is a histamine, putrescine, cadaverine, tyramine and tryptamine. In the practical section BA was determined in lager bottom-fermented beers from local microbreweries and large industrial breweries. Beer is according to legislation considered as a safe food containing a variety of healthful nutrients such as B vitamins, proteins, carbohydrates, minerals, soluble fiber and polyphenolic merged, which have anticarcinogenic, antioxidant and antimicrobial effects. On the other hand, in beer we can find harmful substances such as alcohol, heavy metals, N-nitrosamines, aromatic hydrocarbons, polychlorinated biphenyls, nitrates and biogenic amines. In the second part of the research are summarized the issues of BA in certain foods and beverages, their chemical structure, the way how they are created and their negative effects on the human body. BA are natural, biologically active substances that are essential for the human organism. Their excessive amount in beer is toxic for the human organism. Ethanol contained in the beer decreases the activity of the enzyme monoamine oxidase, and this leads to reduced degradation of BA. Intoxication of BA can cause headaches, vomiting, hypertension, allergic reactions, rashes and even loss of consciousness, therefore, should be the content of these substances monitored. In the practical part of this theses the samples of bottom-fermented beers were analyzed. The first group of samples included unfiltred and unpsaterized beers from microbreweries. In the second group there were filtered and pasteurized beers from large breweries. HPLC determined BA in beer (histamine, putrescine, cadaverine, tyramine and tryptamine). The resulting values indicate that the domestic beers contain biogenic amines in very small concentrations, thus satisfy the legislative standarts. The results were also compared between the two groups of samples. These samples showed that the beer from microbreweries is not contaminated as originally was expected. It shows good technological processes in the manufacture of beer in such small devices.

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