No description available.

Doctor of Philosophy (PhD) / This thesis is an inquiry into the possibility of Aboriginal autonomy under the regime of a state policy which commands self determination. Debate about policy has been dominated by Western scientific, political and professional knowledge, which is challenged by indigenous paradigms grounded in the Dreaming. A recognition of the role of paradox leads me to an attempt at reconciliation between the old and the new Australian intellectual traditions. The thesis advances the theory of internal colonialism by identifying self-determination as its current phase. During more than 200 years of colonial history the relationship between Aborigines and the state has been increasingly contradictory. The current policy of self-determination is a political paradox. Aboriginal people must either conform to the policy by disobeying it, or reject the policy in obedience to it. Through the policy of self-determination the state constructs a relationship of dependent autonomy with Aboriginal people. In a two-year (1994-95) action research project Kitya Aboriginal Health Action Group was set up to empower a local community to establish an Aboriginal health service despite opposition from the Government Health Service. In collaboration with local general practitioners and volunteers the action group opened a health centre. After the end of formal field work government funding and support for the health service was granted. The project illustrated the paradox of dependent autonomy. What appeared as successful community development was not development, and what appeared as destructive factionalism was empowering. Strategies for change made use of contradictions and paradoxes within the state. As an innovation in the practice of social change, the thesis begins the construction of a model for indigenous community action for self-determination in health.

The nature of human well-being has been debated in psychological research since the beginning of the study of human behaviour. Mechanistic perspectives regard humans to be independent objects motivated by external contingencies, with needs to be both self sufficient and self-enhancing. Organismic perspectives describe humans as having innate self-organisational tendencies, which partly depend on qualities of relationships with others. Basic needs for well-being include being self-determined and socially integrated. Both perspectives claim empirical support. Drawing on Self-Determination Theory (SDT), the present study proposed that basic needs for well-being include autonomy and relatedness, and ego-defensive needs that undermine health include independence, control and narcissism. To compare the effects of different needs on well-being, the present study measured people's perceived needs and the satisfaction of those needs, within the context of interpersonal interactions. Participants were sourced from the student population at a Melbourne university (N = 82), and various internet website forums (N= 171). Included were 82 participants who had received a diagnosis of social anxiety in the past two years. A new measure, the Interpersonal Needs Scale (INS) was developed to tap the strength of values for the five needs of interest, and the degree to which needs are satisfied. Factor analysis on the INS produced four value subscales: these were named Authenticity and Belonging, comprising autonomy and relatedness items, and Independence and Narcissism, both including Control items. The fmal version of the INS showed satisfactory reliability and validity. Results for Study 1 indicated that for the present sample, Authenticity and Belonging values were associated with overall interpersonal need satisfaction and with well-being. Conversely, Independence and Narcissistic values were associated with dissatisfaction of interpersonal needs and compromised well-being. For Study 2, cluster analysis was used to group participants according to their INS profiles: that is, similarities in their perceived needs and their degree of satisfaction of needs. In line with predictions, the groups included: the Selfother Balanced (N = 42), who reported significantly greater values for authenticity and belonging over independence and narcissism, and overall need satisfaction; the Slightly Lonely (N = 53), who reported similar value ranking but some dissatisfaction of needs; the Satisfied Narcissists (N = 45), who reported high values for ego-defensive needs and satisfaction of narcissistic needs only; the Needy Narcissists (N= 81), who also reported high values for ego-defensive needs but high overall need dissatisfaction; and the Individualists (N = 27), who reported low values for belonging and unsatisfied independence needs. The characteristics of each group were analysed and compared with each other according to a range of self-concept measures (autonomous-self, relational self, independent-self, and narcissistic personality), indicators of psychological wellbeing (depression, anxiety, stress, and self-esteem), and social well-being (alienation). The Self-other Balanced group reported the highest well-being scores and a predominantly autonomous self-concept, while those who reported low values for belonging needs (Individualists), and those who reported the least satisfaction of interpersonal needs (Needy Narcissists), reported the most compromised well-being. A large proportion of respondents with social anxiety belonged to the latter group. The implications of these results for understanding the impact of values and the satisfaction of interpersonal needs on well-being were discussed.

Abstract There are a remarkable variety of sex determination systems among different animal taxa. In most animals, sex is determined chromosomally. Although in an increasing number of animals sex determination has been found to be influenced primarily by the environment. Species with genotypic sex determination (GSD) have their sex determined at the time of fertilization, by genetic factors alone and those with environmental sex determination (ESD) have their sex determined by environmental factors that act after fertilization. Temperature-dependent Sex Determination (TSD), whereby the sex of the developing embryos depends on the temperature at which they develop is widespread in oviparous reptiles and occurs in all crocodilians, marine turtles and tuatara examined to date and is common in many freshwater turtles and lizards. SECTION ONE Temperature-dependent sex determination (TSD) was never expected to occur in viviparous reptiles, as thermoregulation by pregnant females would result in relatively stable gestation temperatures. Temperature-dependent sex determination and viviparity goes against all the basic assumptions that TSD occurs in oviparous reptiles where temperatures within a nest vary widely. However, skewed sex ratios as a result of incubation temperature indicated the possibility of TSD in the viviparous lizard Eulamprus tympanum. In my first experiments I show the first recorded case of a viviparous reptile with TSD. The developing embryos of the viviparous skink E. tympanum are subject to TSD, with gestation temperature having a highly significant effect on sex and warmer temperatures giving rise to male offspring (Chapter 1). Sex is fully determined at the time of birth and can be differentiated histologically into testes or ovaries (Chapter 2). The morphology and histological characteristics of the gonads of neonatal E. tympanum resulting from the treatment temperatures described in chapter 1 illustrate that sex in E. tympanum is easily distinguished at the time of birth and corresponds with the presence or absence of hemipenes. Males are histologically characterised by an elongated gonad consisting of seminiferous tubules with either no cortical epithelium or, if present at all, in a very thin band. If they are present, M�llerian ducts, showing signs of degeneration, are attached to the kidney by a shortened mesosalpinx. Females are histologically characterised by an irregularly shaped gonad consisting of a thick cortical epithelium that occasionally contains oocytes. The M�llerian ducts are obvious structures attached to the kidney by a fibrous mesosalpinx. The presence or absence of hemipenes is a reliable technique for determining sex in newborn E. tympanum. Sex determination is easiest to perform on neonates within the first few days of birth as hemipenes become increasingly difficult to evert as neonates age, however, with practice they are easily identified without full eversion. SECTION TWO The thermal biology of E. tympanum in the field is restricted by both the thermal properties of their habitat (Chapter 3) and behavioural modifications when faced with a predation threat (Chapter 4). The available temperatures in the field suggest that TSD is biologically relevant in the species and not just a laboratory artefact; E. tympanum can attain mean selected temperatures achieved in the laboratory but the proportion of time at the temperature is restricted. Females actively thermoregulate in the field, although they are restricted in their efficiency of thermoregulation by environmental constraints, for example, microhabitat structure, weather conditions, predator avoidance and social ranking. The highly territorial nature and high densities of E. tympanum present in Kanangra Boyd National Park potentially force less dominant individuals into less favourable habitats that are significantly cooler. An important point is that gravid females in more favourable habitats in the period encompassing the middle third of development (the assumed sex determining period) are selecting higher temperatures, with lower variance and have greater thermoregulatory efficiency than during the rest of pregnancy, therefore, thermoregulating more precisely during this thermosensitive period (Chapter 3). Chemosensory cues provide important information on the risk of predation. Hence, chemoreception is a common mechanism used by many species to detect the presence of, and subsequently respond to, a potential predator. The perceived risk of predation may force retreat to sub-optimal conditions, forcing a trade-off between the risk of predation and the ability to acquire resources. The basking regime maintained by gravid female E. tympanum, can directly alter sex ratios of offspring produced through temperature-dependent sex determination (Chapter 1). The avoidance of predator scents may restrict basking ability and in turn alter the sex of offspring produced. I measured responsiveness to chemical cues using tongue flicks as an indicator of chemical discrimination in females of different reproductive condition. I then measured activity and basking behaviour of gravid and non-gravid females in experimental enclosures in the presence of various chemical stimuli to determine if basking opportunity is compromised by the presence of a predator scent. Females respond differently depending upon reproductive condition, with gravid females responding most significantly to a predator scent. Activity, basking frequency, and time spent in the open (basking duration) are significantly reduced in gravid females in the presence of a predator stimulus. Under laboratory conditions, gravid females modify their behaviour and forego the opportunity to bask when there is a perceived predation risk (Chapter 4). SECTION THREE As female viviparous reptiles can regulate the temperature of the embryo by maternal temperature selection (Chapter 1), the occurrence of TSD in E. tympanum opens the possibility for females to select the sex of offspring. Reproducing females may benefit by facultatively adjusting their investment into sons over daughters or vice versa, in response to population wide shifts in adult sex ratios. Female E. tympanum, can manipulate the sex of their offspring in response to sex imbalances in the population using temperature-dependent sex determination (Chapter 5). When adult males are scarce, females produce male-biased litters and when adult males are common, females produce female-biased litters. The cues used by a female to assess the adult population are not known, but presumably depends upon the female�s experience throughout the breeding season and is the subject of further investigation (Chapter 6). The maternal manipulation of offspring sex ratio in E. tympanum suggests a selective advantage of temperature-dependent sex determination. Any facultative sex ratio response needs to recognise the scarcity of one sex in order to overproduce that sex in the next generation; offspring sex ratio will vary inversely with adult sex ratio. Maternal sex allocation in E. tympanum is linked with population (or adult) sex ratio (Chapter 5), and one of the mechanisms by which females recognise an imbalance may be linked to visual recognition of males (Chapter 6). Females maintained throughout pregnancy without any male stimulus produce entirely male offspring (Chapter 5). In contrast females exposed to male stimulus produce both sexes (Chapter 5). Females respond differently to varying degrees of male stimulus and visual recognition of males in a population may be more important than chemoreception. In the absence of visual cues, females produce more male offspring, even when chemosensory cues are present (Chapter 6). The study system presented here offers many advantages over oviparous species with TSD, due to E. tympanum being relatively short lived and fast maturing. Thus, the fitness consequences over multiple generations as a result of gestation can be investigated. Viviparity allows maternal control of embryonic temperature during gestation and a means of maternal sex allocation. Until now the maternal side of TSD and sex allocation has been where the mother deposits her eggs and the allocation of sex steroid hormones at oviposition, both of which have been difficult to study. The work presented and the study system itself should inspire great interest in TSD and viviparous reptiles.

Grounded in self-determination theory (SDT; Deci & Ryan, 1985), the motivation of sports coaches and the development of the Coach Motivation Questionnaire (CMQ) are the subject of this thesis. Motivation contributes to the way in which people think, feel, and act, and is therefore a central issue in the study of psychology (Ryan & Deci, 2000b). The motivational sequence (Vallerand, 1997) suggests that coaches’ motivations influence their behaviours, which subsequently have an effect on their athletes’ perceptions of the motivational climate. In the sport domain, the coach-athlete relationship is one of the most important influences on athletes’ motivation and subsequent performance (Mageau & Vallerand, 2003). Nevertheless, while there is much research concentrating on the impact of coaching behaviours on athletes’ perceptions and performance, little thought seems to have been given to the coaches’ needs themselves (Mageau & Vallerand, 2003). Following previous work with athletes (e.g., Frederick-Recascino & Schuster-Smith, 2003), the motives and motivational styles of coaches should be related to how they behave within the coaching environment (Frederick & Morrison, 1999). In other words, why coaches coach should influence how coaches coach. The present investigation was conducted to further research and understanding of coach motivation. In the pursuit of this aim, the following three studies were conducted. In Study 1, the motivation of Australian coaches was examined, with a view to informing the development of a valid measurement tool. Employing a qualitative methodology, 13 coaches from different sports and levels of competition participated in semi-structured interviews. Inductive content analysis of the interview transcripts yielded four key dimensions: (a) connection with sport, (b) coach and athlete development, (c) external influences, and (d) internal motives. Overall, SDT appeared to be a useful lens for understanding the data and the existence of multiple forms of motivation were discussed. Study 2 involved the development and pilot testing of a pool of potential items for the CMQ. These items reflected each of the six forms of motivation as conceptualised in SDT, and were designed to be broad and applicable to a wide range of coaches and coaching scenarios. Coaches (N = 86) completed the extended version of the CMQ online, and were requested to respond to two open-ended questions designed to gauge the face validity and general clarity of the questionnaire content. Results suggested that motivation factors were internally consistent, however empirical and qualitative feedback suggested the adaptation of one item for conceptual clarity and the removal of a second item resulting in the CMQ-41. The psychometric properties of the CMQ were examined in Study 3. Coaches (N = 556) completed an online questionnaire package that included the CMQ and measures of psychological needs, goal orientation, and well-being. Examination using CFA produced a 22-item measure with good model fit. Further evaluation produced preliminary evidence for the scale’s validity and reliability. The implications of these results for researchers and practitioners are discussed. Directions for research concerning coach motivation, SDT, and the coach-athlete performance relationship are also highlighted.

This study investigated the perceived locus of causality of motivation in high school football players. The Sports Motivation Scale (SMS) was used to study seven motivation subscales (IM to know, IM to accomplish, IM stimulation, EM identified, EM introjected, EM external and Amotivation) with respect to motivation among high school football players. This indicated that IM to know, IM to accomplish, EM identified and EM introjected were the best predictors of the participants? perceived locus of causality of motivation. The results indicate that when using perceived locus of causality for motivation, position played could be predicted 62 percent of the time. The study also speculates on ways in which communication could be used to affect motivation.

Feedback sign (positive, negative, or no feedback sign) and feedback style (autonomous, controlling, or no feedback style) were manipulated in a 3x3 repeated measures design. Two hundred thirty-three undergraduate students from introductory psychology classes completed measures of perceived competence, perceived choice, and interest over four time periods. Interest was regressed on perceived competence, perceived choice, and a moderation analysis revealed that perceived choice moderated the effect of perceived competence on interest. Creative answers to open-ended problems were assessed after time 2 (before feedback), and after time 3 (after feedback). Feedback style (autonomous, controlled, and neutral) and Feedback sign (positive, negative, and neutral) manipulations were analyzed using a 3x3 ANOVA, revealing no effect of feedback. Post-hoc analyses using perceived difficulty of the first creative problem as a covariate revealed an interaction of feedback style and difficulty, limiting between subjects analyses. Creativity was also regressed on interest. Pre-feedback interest predicted creativity according to expectations, but post-feedback interest did not predict creativity. Creativity did predict post-performance interest, possibly implying that interest is not a valid proxy for intrinsic motivation in within-subjects designs. Future studies should test the proposition that feedback affects intrinsic motivation, which in turn affects creative performance, and creative performance affects interest.

The developmental period of adolescence typically refers to the years between 13 and 19, and is associated with developmental tasks that help youth become young adults. The transition to adulthood is typically recognized by common adulthood benchmarks such as leaving home, finishing school, marriage, financial independence and having children. However, many young men and women attending college remain financially and emotionally dependent on their parents, as they have not entered the professional work ranks and are faced with the challenges of college. Increasingly, colleges and universities are becoming places to help teach young people to become prepared for the professional ranks and engaged with the world that surrounds them. However, very little research in higher education is focused on the developmental benefits associated with the college experience. The purpose of this study was to examine the presence of developmental supports for self-determination and initiative in a student leadership program. Throughout the youth development literature, self-determination and initiative are recognized as important internal capacities that aid young people as they transition to adulthood. These concepts provide the theoretical lens for a qualitative case study of a college leadership group. Data were gathered through in-depth semi-structured interviews, observations, a year end focus group, and supplemented by a review of the organizational instruments and tools they develop. Findings from this study confirm past studies of youth development organizations and extend this work by applying it to the developmental period of emerging adulthood. For the leadership group under investigation, initiative and self-determination were supported primarily through the actions of peers within the group. The experience of student leaders often shaped how the group was led, and these leaders became an important source of support for the basic needs of relatedness, competence, and autonomy within the group. The study covers a three-year period, and contrasts how peer leadership changed and impacted group functioning and performance over time. Practical implications of the study relate to the important role of faculty and graduate student advisors in training and monitoring student leaders before these individuals take a formal leadership role for these groups.

This thesis describes the study of the three-dimensional structure and dynamics of the hepatitis B virus (HBV) encapsidation signal, epsilon, by means of nuclear magnetic resonance (NMR) and mutational data. HBV replicates by reverse transcription of an RNA pregenome into the viral DNA genome, which becomes enclosed in viral particles (encapsidation). Epsilon is a stem-loop structure within the RNA pregenome and both the primary sequence and secondary structure of epsilon are strongly conserved, in agreement with its essential function of propagating HBV. Epsilon is therefore a potential target for drug design. Studying the structure of epsilon requires development of new methods in the field of structural biology, as it is such a large RNA. Knowing the structure of epsilon will help to better understand the encapsidation mechanism and priming step of reverse transcription. This will help us in the search for antiviral drugs that block epsilon and prevent the viral reverse transcriptase from binding. NMR spectroscopy is a method that provides detailed structural and dynamical data in solution under natural conditions. However, the size of the molecules that can be studied with NMR is limited. NMR spectra become more and more difficult to interpret as the size of the molecule increases. To circumvent this problem, large RNA molecules can be divided into smaller parts and only the parts essential for NMR studies are selected. The information obtained from these smaller fragments can then be used to determine the structure of the larger molecule. Furthermore, a new method of enzymatically synthesizing nucleoside triphosphates with isotopes suitable for NMR has made it possible to specifically label the RNA molecules. Using this method it is possible to derive highly detailed molecular structures of RNA up to a size of 150 nucleotides. The method of selective isotope labelling was applied to different parts of HBV epsilon. Three RNA fragments of 27 (apical loop), 36 (internal bulge) and 61 (whole epsilon) nucleotides (nt) were synthesized in the unlabelled form. The 27-nt and 36-nt RNAs were also synthesized with (13C, 15N, 1', 3', 4', 5', 5"-2H5)-labelled uridines. The 61-nt sequence was (13C, 15N)-guanidine labelled. This labelling allowed unambiguous assignment of otherwise inaccessible parameters. The unlabelled and labelled RNA sequences provided the necessary data for structure derivation of the whole epsilon. The apical loop of epsilon forms a pseudo-triloop motif. There is only one conformation of the loop that fulfils all the restraints, including experimental chemical shifts. However, the loop adopts several structures that fulfil the experimental distance, torsion angle and residual dipolar coupling restraints. This may reflect true flexibility. Indeed, relaxation studies on the unlabelled and labelled 27-nt sequences show that the residues that show multiple conformations are flexible. This can be an important feature for the recognition and subsequent binding of epsilon to the viral polymerase. The information gained on the HBV encapsidation signal is useful in our understanding of the initiation of replication of the virus. This can in turn contribute to the search for drugs against HBV.

The use of bioenergy has been increasing due to efforts in fossil fuels replacement. Modern bioenergy technologies aim for high efficiency and low pollution levels, which increases the need for methods for the on-line characterization of biofuels. Dielectric methods have been identified as useful for the sensing of solid biofuels because they allow for rapid, nonhazardous, nondestructive, and bulk determination of material properties. The dielectric properties describe the interaction between the material and the electromagnetic waves. Dielectric properties are intrinsic of the materials and can therefore be used for the development of prediction models that can be applied regardless of the measurement technique. The study of the dielectric properties is also important as it improves the understanding of the dielectric behavior of the materials. This thesis focuses on the dielectric properties of solid biofuels and their use in the characterization of these materials. The work presented includes the development of new methods permitting the determination of the dielectric properties of solid biofuels with large particle size (waveguide method), broadband measurement of the dielectric properties (coaxial-line probe), and the use of a previously developed method for the accurate determination of the dielectric properties (free-space method). The results includes the dielectric properties of solid biofuels and their dependence on parameters such as frequency, moisture, density, and temperature. This thesis also presents semi-theoretical models for the determination of moisture content, which obtained a RMSEP of 4% for moisture contents between 34 and 67%, and an empirical model that resulted in a RMSEC of 0.3% for moisture contents between 4 and 13%. Finally, this thesis includes measurements of the influence of salt content on the dielectric properties and a discussion of its use for estimation of the ash content of solid biofuels.