Child-directed speech (CDS) differs from adult-directed speech (ADS) in several respects whose possible facilitating effects for language acquisition are still being studied. One such difference concerns articulation rate --- the number of linguistic units by the number of time units, excluding pauses --- which has been shown to be generally lower than in ADS. However, while it is well-established that ADS exhibits an inverse relation between articulation rate and information-theoretic surprisal --- the amount of information encoded in a linguistic unit --- this measure has been conspicuously absent in the study of articulation rate in CDS. Another issue is if the lower articulation rate in CDS is stable across utterances or an effect of local variation, such as final lengthening. The aim of this work is to arrive at a more comprehensive model of articulation rate in CDS by including surprisal and final lengthening. In particular, one-word utterances were studied, also in relation to word-length effects (the phenomenon that longer words generally have a higher articulation rate). To this end, a methodology for large-scale automatic phoneme-alignment was developed, which was applied to two longitudinal corpora of Swedish CDS. It was investigated i) how articulation rate in CDS varied with respect to child age, ii) whether there was a negative relation between articulation rate and surprisal in CDS, and iii) to what extent articulation rate was lower in CDS than in ADS. The results showed i) a weak positive effectof child age on articulation rate, ii) a negative relation between articulation rate and surprisal, and iii) that there was a lower articulation rate in CDS but that the difference could almost exclusively be attributed to one-word utterances and final lengthening. In other words, adults seem to adapt how fast they speak to their children's age, speaking faster to children is correlated with a reduced amount of information, and the difference in articulation rate between CDS and ADS is most prominent in isolated words and final lengthening. More generally, the results suggest that CDS is well-suited for word segmentation, since lower articulation rate in one-word utterances provides an additional cue.

Dissertation (Ph.D.)--University of South Florida, 2007. / Title from PDF of title page. Document formatted into pages; contains 197 pages. Includes vita. Includes bibliographical references.

Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 4 uppsatser.

Dissertation (Ph.D.)--University of South Florida, 2007. / Includes vita. Includes bibliographical references (leaves 188-197). Also available online.

Thesis (Ed. S.)--Miami University, Dept. of Educational Psychology, 2009. / Title from first page of PDF document. Includes bibliographical references (p. 22-25).

Thesis (Ph. D.)--University of Oklahoma. / Bibliography: leaves 116-129.

Thesis (M.S.)--Miami University, Dept. of Chemistry and Biochemistry, 2004. / Title from first page of PDF document. Includes bibliographical references (p. 57-64).

<p>Gene regulatory networks (GRNs) provide a systems-level orchestration of an organism’s genome encoded anatomy. As biological networks are revealed, they continue to answer many questions including knowledge of how GRNs control morphogenetic movements and how GRNs evolve. Morphogenesis is a complex orchestration of movements by cells that are specified early in development. </p><p> The activation of an upstream GRN is crucial in order to orchestrate downstream morphogenetic events. In the sea urchin, activation of the endomesoderm GRN occurs after the asymmetric 4th cleavage. Embryonic asymmetric cell divisions often are accompanied by differential segregation of fate-determinants into one of two daughter cells. That asymmetric cleavage of the sea urchin micromeres leads to a differential animal-vegetal (A/V) nuclear accumulation of cell fate determinants, β-Catenin and SoxB1. Β-Catenin protein is localized into the nuclei of micromeres and activates the endomesoderm gene regulatory network, while SoxB1 is excluded from micromeres and enters the nucleus of the macromeres, the large progeny of the unequal 4th cleavage. Although nuclear localization of β-Catenin and SoxB1 shows dependence on the asymmetric cleavage, the mechanics behind the asymmetrical division has not been demonstrated. In Chapter 3, we show that micromere formation requires the small RhoGTPase, Cdc42 by directing the apical/basal orientation of the mitotic spindle at the apical cortex. By attenuating or augmenting sea urchin Cdc42 function, micromere divisions became defective and failed to correctly localize asymmetrically distributed determinants. As a consequence, cell fates were altered and multiple A/V axes were produced resulting in a “Siamese-twinning” phenotype that occurred with increasing frequency depending on the quantitative level of perturbation. Our findings show that Cdc42 plays a pivotal role in the asymmetric division of the micromeres, endomesoderm fate-determinant segregation, and A/V axis formation.</p><p> This dissertation also characterizes, at high resolution, the repertoire of cellular movements contributing to three different morphogenetic processes of sea urchin development: the elongation of gut, the formation of the primary mouth, and the migration of the small micromeres (the presumptive primordial germ cells) in the sea urchin, Lytechinus variegatus. Descriptive studies of the cellular processes during the different morphogenetic movements allow us to begin investigating their molecular control. </p><p>In Chapter 4, we dissected the series of complex events that coordinate gut and mouth morphogenesis. Until now, it was thought that lateral rearrangement of endoderm cells by convergent extension was the main contributor to sea urchin archenteron elongation and that cell divisions were minimal during elongation. We performed cell transplantations to live image and analyze a subset of labeled endoderm cells at high-resolution in the optically clear sea urchin embryo. We found that the endomesoderm cells that initially invaginate into the sea urchin blastocoel remained contiguous throughout extension, so that, if convergent extension were present, it was not a major contributor to elongation. We also found a prevalence of cell divisions throughout archenteron elongation that increased the number of cells within the gut linearly over time; however, we showed that the proliferation did not contribute to growth, and their spindle orientations were randomized during divisions and therefore did not selectively contribute to the final gut length. When cell divisions were inhibited, we saw no difference in the ability of the cells within the gut to migrate in order to elongate. Also in Chapter 4, we describe our observations of the cell biological processes underlying primary mouth formation at the end of gastrulation. Using time-lapse microscopy, photo-convertible Kaede, and an assay of the basement membrane remodeling, we describe a sequential orchestration of events that leads to the fusion of the oral ectoderm and the foregut endoderm. Our work characterizes, at higher resolution than previously recorded, the temporal sequence and repertoire of the cellular movements contributing to the length of the sea urchin larval gut and tissue fusion with the larval primary mouth.</p><p> In Chapter 5, the migration of the small micromeres to the coelomic pouches in the sea urchin embryo provides an exceptional model for understanding the genomic regulatory control of morphogenesis. An assay using the robust homing potential of these cells reveals a “coherent feed-forward” transcriptional subcircuit composed of Pax6, Six3, Eya, and Dach1 that is responsible for the directed homing mechanism of these multipotent progenitors. The linkages of that circuit are strikingly similar to a circuit involved in retinal specification in Drosophila suggesting that systems-level tasks can be highly conserved even though the tasks drive unrelated processes in different animals.</p><p> The sea urchin gene regulatory network (GRN) describes the cell fate specification of the developing embryo; however, the GRN does not describe specific cell biological events driving the three distinct sequences of cell movements. Our ability to connect the GRN to the morphogenetic events of gastrulation, primary mouth formation, and small micromere migration will provide a framework for characterizing these remarkable sequences of cell movements in the simplest of deuterostome models at an unprecedented scale.</p> / Dissertation

The closed neighbourhood of a vertex in a graph is the vertex together with the set of adjacent vertices. A di®erentiating-dominating set, or identifying code, is a collection of vertices whose intersection with the closed neighbour- hoods of each vertex is distinct and nonempty. A di®erentiating-dominating set in a graph serves to uniquely identify all the vertices in the graph. Chapter 1 begins with the necessary de¯nitions and background results and provides motivation for the following chapters. Chapter 1 includes a summary of the lower identi¯cation parameters, °L and °d. Chapter 2 de- ¯nes co-distinguishable graphs and determines bounds on the number of edges in graphs which are distinguishable and co-distinguishable while Chap- ter 3 describes the maximum number of vertices needed in order to identify vertices in a graph, and includes some Nordhaus-Gaddum type results for the sum and product of the di®erentiating-domination number of a graph and its complement. Chapter 4 explores criticality, in which any minor modi¯cation in the edge or vertex set of a graph causes the di®erentiating-domination number to change. Chapter 5 extends the identi¯cation parameters to allow for orientations of the graphs in question and considers the question of when adding orientation helps reduce the value of the identi¯cation parameter. We conclude with a survey of complexity results in Chapter 6 and a collection of interesting new research directions in Chapter 7. / Mathematical Sciences / PhD (Mathematics)