The diploma thesis deals with self-healing of concrete composite. In the theoretical part, it represents the effect of polymer microfibers on reducing the development of cracks and on improving self-healing properties. It also describes the most commonly used types of bacteria used to enhance the self-healing process and their effect both alone and in interaction with fibers, calcium sources and absorbent additives. It also represents auxiliary additives in the form of superabsorbent polymers. In the practical part, it verifies the influence of the above elements and describes how much the self-healing of concrete has been enhanced by the use of combinations of fibers, absorbent polymers and a source of calcium in the form of calcium lactate. It also indicates whether the self-healing properties used were not improved at the expense of deteriorating the mechanical properties of the composite.