Digital image processing techniques can be used to correct the distortions produced on a ship's sonar display that are caused by some of the components of the rotational movement of the ship.

abstract: Cyanovirin-N (CV-N) is a naturally occurring lectin originally isolated from the cyanobacteria Nostoc ellipsosporum. This 11 kDa lectin is 101 amino acids long with two binding sites, one at each end of the protein. CV-N specifically binds to terminal Manα1-2Manα motifs on the branched, high mannose Man9 and Man8 glycosylations found on enveloped viruses including Ebola, Influenza, and HIV. wt-CVN has micromolar binding to soluble Manα1-2Manα and also inhibits HIV entry at low nanomolar concentrations. CV-N's high affinity and specificity for Manα1-2Manα makes it an excellent lectin to study for its glycan-specific properties. The long-term aim of this project is to make a variety of mutant CV-Ns to specifically bind other glycan targets. Such a set of lectins may be used as screening reagents to identify biomarkers and other glycan motifs of interest. As proof of concept, a T7 phage display library was constructed using P51G-m4-CVN genes mutated at positions 41, 44, 52, 53, 56, 74, and 76 in binding Domain B. Five CV-N mutants were selected from the library and expressed in BL21(DE3) E. coli. Two of the mutants, SSDGLQQ-P51Gm4-CVN and AAGRLSK-P51Gm4-CVN, were sufficiently stable for characterization and were examined by CD, Tm, ELISA, and glycan array. Both proteins have CD minima at approximately 213 nm, indicating largely β-sheet structure, and have Tm values greater than 40°C. ELISA against gp120 and RNase B demonstrate both proteins' ability to bind high mannose glycans. To more specifically determine the binding specificity of each protein, AAGRLSK-P51Gm4-CVN, SSDGLQQ-P51Gm4-CVN, wt-CVN, and P51G-m4-CVN were sent to the Consortium for Functional Glycomics (CFG) for glycan array analysis. AAGRLSK-P51Gm4-CVN, wt-CVN, and P51G-m4-CVN, have identical specificities for high mannose glycans containing terminal Manα1-2Manα. SSDGLQQ-P51Gm4-CVN binds to terminal GlcNAcα1-4Gal motifs and a subgroup of high mannose glycans bound by P51G-m4-CVN. SSDGLQQ-wt-CVN was produced to restore anti-HIV activity and has a high nanomolar EC50 value compared to wt-CVN's low nanomolar activity. Overall, these experiments show that CV-N Domain B can be mutated and retain specificity identical to wt-CVN or acquire new glycan specificities. This first generation information can be used to produce glycan-specific lectins for a variety of applications. / Dissertation/Thesis / Ph.D. Biochemistry 2013

The generation of high affinity, selective, and in vivo-stable peptide-based drugs is currently a major challenge in the field of drug development. Technologies exist that permit the generation of a vast diversity of chemical and conformational space and an example of such a technology is mRNA display, which utilizes protein translation machinery to produce a wide array of polypeptides starting from a combinatorial library of mRNA templates. The intention of this research was to bridge mRNA display to a reconstituted translation system using protein synthesis using recombinant elements (PURE) system for a new drug discovery platform. We hypothesized that it is possible to generate mRNA-peptidomimetic fusions using reconstituted translation system and chemo-enzymatically charged tRNAs, to incorporate unnatural amino acids into mRNA-peptidomimetic fusions. Upon demonstating that the reconstituted system was functional, we have synthesized hexapeptide fusion products containing four alanine residues and one biocytin residue. Fusions were assayed using urea-PAGE in the presence of streptavidin which allowed for unambiguous evaluation of the full length fusion fraction. It was determined that overall more fusion product was generated with template that codes for biocytin early in the coding sequence, but that the percent of biocytin-containing product stays similar regardless of the biocytin place in the coding region. We have also found that the change in template untranslated region length does not improve incorporation of biocytin in dipeptide fusions within the tested range. Finally, after first unsuccessful attempts to make sarcosine hexapeptide fusions, we investigated the effect of magnesium ion concentration on the translation reaction. As a result of four series of experiments performed involving both alanine and sarcosine fusion synthesis in parallel, we concluded that an increase in magnesium concentration from 5 mM to 20 mM coincided with enabling of the reconstituted system in making hexapeptide fusions with sarcosine in a significantly high number of cases. This research work arises from the need to enable a new drug discovery tool that will allow both synthesis and affinity maturation of peptide-based compounds. It represents our pioneering efforts to develop a new technology and ultimately help bring to existence compounds of significant therapeutic value. / Pharmaceutical Sciences, Faculty of / Graduate

Lacome is a multi-user cross-platform system that supports collaboration in a shared large screen display environment. Lacome allows users to share their desktops or application windows using any standard VNC server. It supports multi-user concurrent interaction on the public shared display as well as input redirection so users can control each other's applications. La-come supports separate types of interaction through a Lacome client for window management tasks on the shared display(move, resize, iconify, de-iconify) and for application interactions through the VNC servers. The system architecture provides for Publishers that share information and Navigators that access information. A Lacome client can have either or both, and can initiate additional Publishers on other VNC servers that may not be Lacome clients. Explicit access control policies on both the server side the client side provide a flexible framework for sharing. The architecture builds on standard cross-platform components such as VNC and JRE. Interaction techniques used in the window manager ensure simple and transparent multi-user interactions for managing the shared display space. We illustrate the design and implementation of Lacome and provide insights from initial user experience with the system. / Science, Faculty of / Computer Science, Department of / Graduate

The study investigated grade 6 students' comprehension of narrative text when presented on a computer and as printed words on paper. A set of comprehension tests were developed for three stories of varying length (382 words, 1047 words and 1933 words) using a skills hierarchy protocol. The text for each story was prepared for presentation on a Macintosh computer using a program written for the study and as print in the form of exact copies of the computer screen. Students from two grade 6 classes in a suburban elementary school were randomly assigned to read one of the stories in either print form or on the computer and subsequently completed a comprehension test as well as a questionnaire concerning attitude and personal information. The responses from the comprehension tests were evaluated by graduate students in Language Education. The data evolved from the tests and questionnaires were analysed to determine measures of test construct validity, inter-rater reliability, and any significant difference in the means of comprehension scores for the two experimental groups for each story. The results indicated small but insignificant differences between the means of the three comprehension test scores for computer and print. A number of students reading from the computer complained of eye fatigue. The scores of subjects reading the longest story and complaining of eye fatigue were significantly lower. / Education, Faculty of / Curriculum and Pedagogy (EDCP), Department of / Graduate

No description available.

Laser-based projection displays have been under active development over last few years. Lasers are considered as the next generation light sources for projection due to advantages including wide color gamut, high brightness, long lifetime and high electric-optic efficiency. However, speckle phenomenon caused by the coherence of lasers can greatly influence the quality of projected images. A cost effective, easy setup and robust speckle reduction system for laser projection is still very challenging. The aim of this thesis is to investigate practical speckle reduction solutions for laser projection system. The system is divided into different modules and for each module, speckle reduction methods are studied. The study includes a low speckle laser source, an optical engine with integrated speckle reduction methods and a low speckle engineered screen. A standard speckle measurement system corresponding to human perception is also presented. For low speckle laser source, a theoretical model that describes wavelength diversity in speckle reduction is established. The speckle contrast ratio (SCR) of the system can be simulated. The result is experimentally verified. Guidelines for optimized wavelength/power selection for lasers to generate reduced speckle are proposed. After this, a low cost, high compact and high-efficiency speckle reduction optical system using an electric elastomer actuator is studied. Both theoretical analysis and experimental work are presented and a SCR as low as 3.7% was achieved. Besides these two parts, an engineered screen that utilizes micro lens array (MLA) which can greatly reduce speckle is studied. A comprehensive theoretical model was established for the simulation and optimization of MLA in speckle reduction. The simulated results agree well with reported values. Guidelines in MLA selection for laser projection are presented. In the last part, a standard measurement setup of speckle in a laser projection system that matches the human perception is studied. Conditions including camera F/#, focal length, integration time and measuring geometry are discussed and suitable setups are proposed. / Thesis / Doctor of Philosophy (PhD)

No / Discovery of new catalysts for demanding aqueous reactions is challenging. Here, we describe methodology for selection of catalytic phages by taking advantage of localized assembly of the product of the catalytic reaction that is screened for. A phage display library covering 109 unique dodecapeptide sequences is incubated with nonassembling precursors. Phages which are able to catalyze formation of the self-assembling reaction product (via amide condensation) acquire an aggregate of reaction product, enabling separation by centrifugation. The thus selected phages can be amplified by infection of Escherichia coli. These phages are shown to catalyze amide condensation and hydrolysis. Kinetic analysis shows a minor role for substrate binding. The approach enables discovery and mass-production of biocatalytic phages.

The research covered in this thesis encompasses a consideration of 3D television requirements and a survey of stereoscopic and autostereoscopic methods. This confirms that although there is a lot of activity in this area, very little of this work could be considered suitable for television. The principle of operation, design of the components of the optical system and evaluation of two EU-funded (MUTED & HELIUM3D projects) glasses-free (autostereoscopic) displays is described. Four iterations of the display were built in MUTED, with the results of the first used in designing the second, third and fourth versions. The first three versions of the display use two-49 element arrays, one for the left eye and one for the right. A pattern of spots is projected onto the back of the arrays and these are converted into a series of collimated beams that form exit pupils after passing through the LCD. An exit pupil is a region in the viewing field where either a left or a right image is seen across the complete area of the screen; the positions of these are controlled by a multi-user head tracker. A laser projector was used in the first two versions and, although this projector operated on holographic principles in order to obtain the spot pattern required to produce the exit pupils, it should be noted that images seen by the viewers are not produced holographically so the overall display cannot be described as holographic. In the third version, the laser projector is replaced with a conventional LCOS projector to address the stability and brightness issues discovered in the second version. In 2009, true 120Hz displays became available; this led to the development of a fourth version of the MUTED display that uses 120Hz projector and LCD to overcome the problems of projector instability, produces full-resolution images and simplifies the display hardware. HELIUM3D: A multi-user autostereoscopic display based on laser scanning is also described in this thesis. This display also operates by providing head-tracked exit pupils. It incorporates a red, green and blue (RGB) laser illumination source that illuminates a light engine. Light directions are controlled by a spatial light modulator and are directed to the users’ eyes via a front screen assembly incorporating a novel Gabor superlens. In this work is described that covered the development of demonstrators that showed the principle of temporal multiplexing and a version of the final display that had limited functionality; the reason for this was the delivery of components required for a display with full functionality.

Conventional stereoscopic displays present a pair of stereoscopic images on a single and fixed image plane decoupled with the vergence and accommodation responses of the viewer. In consequence, these displays lack the capability of correctly rendering focus cues (i.e. accommodation and retinal blur) and may induce the discrepancy between accommodation and convergence. A number of visual artifacts associated with incorrect focus cues in stereoscopic displays have been reported, limiting the applicability of these displays for demanding applications and daily usage.In this dissertation, methods and apparatus for generating addressable focus cues in conventional stereoscopic displays are proposed. Focus cues can be addressed throughout a volumetric space, either through dynamically varying the focal distance of a display enabled by an active optical element or by multiplexing a stack of 2-D image planes. Optimal depth-weighted fusing functions are developed to fuse a number of discrete image planes into a seamless volumetric space with continuous and near-correct focus cues similar to the real world counterparts.The optical design, driving methodology, and prototype implementation of the addressable focus displays are presented and discussed. Experimental results demonstrate continuously addressable focus cues from infinity to as close as the near eye distance. Experiments to further evaluate the depth perception in the display prototype are conducted. Preliminary results suggest that the perceived distance and accommodative response of the viewer match with the addressable accommodation cues rendered by the display, approximating the real-world viewing condition.