One way to determine if all students can learn through the use of computers is to introduce a lesson taught completely via computers and compare the results with those gained when the same lesson is taught in a traditional manner. This study attempted to determine if a virtual fetal pig dissection can be used as a viable alternative for an actual dissection for females enrolled in high school biology classes by comparing the knowledge acquisition and attitudinal change between the experimental (virtual dissection) and control (actual dissection) groups. Two hundred and twenty four students enrolled in biology classes in a suburban all-girl parochial high school participated in this study. Female students in an all-girl high school were chosen because research shows differences in science competency and computer usage between the genders that may mask the performance of females on computer-based tasks in a science laboratory exercise. Students who completed the virtual dissection scored significantly higher on practical test and objective tests that were used to measure knowledge acquisition. Attitudinal change was measured by examining the students' attitudes toward dissections, computer usage in the classroom, and toward biology both before and after the dissections using pre and post surveys. Significant results in positive gain scores were found in the virtual dissection group's attitude toward dissections, and their negative gain score toward virtual dissections. Attitudinal changes toward computers and biology were not significant. A purposefully selected sample of the students were interviewed, in addition to gathering a sample of the students' daily dissection journals, as data highlighting their thoughts and feelings about their dissection experience. Further research is suggested to determine if a virtual laboratory experience can be a substitute for actual dissections, or may serve as an enhancement to an actual dissection.

Actin isoform specific mutations have been identified as causes for various human diseases. These include twelve missense mutations in γ-nonmuscle actin leading to early onset autosomal dominate nonsyndromic hearing loss and twenty two missense mutations in α-smooth muscle actin leading to thoracic aortic aneurysms and dissections (TAAD). The molecular mechanisms leading to these human pathologies are mainly unknown, principally due to the inability to isolate pure mutant γ-nonmuscle actin and α-smooth muscle actin in quantities required for biochemical analysis. To begin to address these problems, I have individually expressed the human nonmuscle actin isoforms, β– and γ– nonmuscle actin, in a baculovirus expression system and characterized their biochemical properties. Surprisingly, despite a conserved amino acid difference of only 4 residues at or near the N-terminus, Ca-γ-actin exhibits slower monomeric and filamentous biochemical properties than β-actin. In the Mg-form, the difference between the two is smaller. Mixing experiments with Ca-actins reveal the two will readily co-polymerize. Calcium bound in the high affinity binding site of γ-actin may cause a selective energy barrier relative to β-actin that retards the equilibration between G– and F-monomer conformations resulting in a slower polymerizing actin with greater filament stability. This difference may be particularly important in sites such as the γ-actin-rich cochlear hair cell stereocilium where local mM calcium concentrations may exist. In hair cells γ-nonmuscle actin seems to play a central role in stereocilia maintenance. To determine how the deafness causing D51N-γ-mutant actin mutation leads to deafness, I expressed and characterized it in the γ-actin background. The D51N mutation, lethal when cloned into yeast, displayed decreased filament stability and polymerization kinetics of an actin more dynamic than γ-actin. This result suggests that the hearing effects of the γ-actin mutations on the hearing apparatus are not simply caused by an inability to polymerize. The observed increased polymerization rates and decreased filament stability may have major implications for the human disease, as the mutation may alter the ability of the γ-actin to fulfill its maintenance functions. To address the basis by which TAAD mutations cause vascular dysfunction I introduced two of the know human mutations, N115T and R116Q, into yeast actin, 86% identical to human α-smooth muscle actin. I then generated yeast strains expressing each of these mutations as the sole actin in the cell to assess their effect on actin function in vivo and in vitro. Both mutant strains exhibited reduced ability to grow under a variety of stress conditions, although the N115T cells were more severely affected. In vitro the mutations caused exhibited altered thermostability and nucleotide exchange rates indicating effects on monomer conformation with R116Q the most severely affected. The N115T actin demonstrated a biphasic elongation phase during polymerization, while R116Q actin demonstrated a markedly extended nucleation phase. Allele-specific effects were also seen on critical concentration, rate of depolymerization and filament treadmilling. R116Q filaments were hypersensitive to severing by the actin-binding protein cofilin. In contrast, N115T filaments were hyposensitive to cofilin, despite near normal binding affinities of actin for cofilin. The mutant specific effects on actin behavior suggest that individual mechanisms may contribute to TAAD. Understanding the mechanisms of actin dependent human diseases requires elucidation of the effects of the mutations on the behavior of actin per se, its regulation, and the impact on actin mediated processes within the cell. The work provided in this thesis and future studies will provide the information required to understand the pathways involved in these diseases and form innovative treatments for deafness and TAAD.

Les limites d’échelle de grands arbres aléatoires jouent un rôle central dans cette thèse.Nous nous intéressons plus spécifiquement au comportement asymptotique de plusieurs fonctions codant des arbres de Galton-Watson conditionnés. Nous envisageons plusieurs types de conditionnements faisant intervenir différentes quantités telles que le nombre total de sommets ou le nombre total de feuilles, avec des lois de reproductions différentes.Lorsque la loi de reproduction est critique et appartient au domaine d’attraction d’uneloi stable, un phénomène d’universalité se produit : ces arbres ressemblent à un même arbre aléatoire continu, l’arbre de Lévy stable. En revanche, lorsque la criticalité est brisée, la communauté de physique théorique a remarqué que des phénomènes de condensation peuvent survenir, ce qui signifie qu’avec grande probabilité, un sommet de l’arbre a un degré macroscopique comparable à la taille totale de l’arbre. Une partie de cette thèse consiste à mieux comprendre ce phénomène de condensation. Finalement, nous étudions des configurations non croisées aléatoires, obtenues à partir d’un polygône régulier en traçant des diagonales qui ne s’intersectent pas intérieurement, et remarquons qu’elles sont étroitement reliées à des arbres de Galton-Watson conditionnés à avoir un nombre de feuilles fixé. En particulier, ce lien jette un nouveau pont entre les dissections uniformes et les arbres de Galton-Watson, ce qui permet d’obtenir d’intéressantes conséquences de nature combinatoire. / Scaling limits of large random trees play an important role in this thesis. We are more precisely interested in the asymptotic behavior of several functions coding conditioned Galton-Watson trees. We consider several types of conditioning, involving different quantities such as the total number of vertices or leaves, as well as several types of offspring distributions. When the offspring distribution is critical and belongs to the domainof attraction of a stable law, a universality phenomenon occurs: these trees look like the samecontinuous random tree, the so-called stable Lévy tree. However, when the offspring distributionis not critical, the theoretical physics community has noticed that condensation phenomenamay occur, meaning that with high probability there exists a unique vertex with macroscopicdegree comparable to the total size of the tree. The goal of one of our contributions is to graspa better understanding of this phenomenon. Last but not least, we study random non-crossingconfigurations consisting of diagonals of regular polygons, and notice that they are intimatelyrelated to Galton-Watson trees conditioned on having a fixed number of leaves. In particular,this link sheds new light on uniform dissections and allows us to obtain some interesting resultsof a combinatorial flavor.

This diploma thesis investigates attitudes of students and teachers of secondary schools (gymnasia) towards the use of dissections into educational process. Questionnaire survey is a part of the research, by which opinions about animal dissections are examined in terms of a particular interest in dissections, of general interest in natural sciences, disgust towards the peculiar facts and situations taking place during dissections and finally in terms of ethical standpoint of all respondents. Statistic processing of results examined relations between all presented aspects and between gender, age, school grade and targeting of studies, experience with dissections and interest of all teachers themselves. At the beginning of the inquiry the following research questions were stated: 1. Are attitudes of students towards dissections during the lessons of biology different between genders? 2. Are those attitudes affected by factors such as subject preference, targeted field of study, own experience with dissections, favoured method of teaching anatomy or owning a pet? 3. Do mutual correlations between the attitude of a student and a particular teacher exist? 4. Are standpoints of teachers towards dissections during the lessons of biology influenced by various factors such as implementation into educational...

Cette thèse porte sur le partitionnement parallèle de graphes et essentiellement sur son application à la renumérotation de matrices creuses.<br />Nous utilisons pour résoudre ce problème un schéma multi-niveaux dont nous avons parallélisé les phases de contraction et d'expansion.<br />Nous avons ainsi introduit pour la phase de contraction un nouvel algorithme de gestion des conflits d'appariements distants, tout en améliorant les algorithmes déjà existants en leur associant une phase de sélection des communications les plus utiles.<br />Concernant la phase de d'expansion, nous avons introduit la notion de graphe bande qui permet de diminuer de manière très conséquente la taille du problème à traiter par les algorithmes de raffinement. Nous avons généralisé l'utilisation de ce graphe bande aux implantations séquentielles et parallèles de notre outil de partitionnement Scotch.<br />Grâce à la présence du graphe bande, nous avons proposé une utilisation nouvelle des algorithmes génétiques dans le cadre de l'expansion en les utilisant comme heuristiques parallèles de raffinement de la partition.

Cette thèse porte sur le partitionnement parallèle de graphes et essentiellement sur son application à la renumérotation de matrices<br />creuses.<br /><br />Nous utilisons pour résoudre ce problème un schéma multi-niveaux dont nous avons parallélisé les phases de contraction et d'expansion.<br /><br />Nous avons ainsi introduit pour la phase de contraction un nouvel algorithme de gestion des conflits d'appariements distants, tout en<br />améliorant les algorithmes déjà existants en leur associant une phase<br />de sélection des communications les plus utiles.<br /><br />Concernant la phase d'expansion, nous avons introduit la notion de graphe bande qui permet de diminuer de manière très conséquente la taille du problème à traiter par les algorithmes de raffinement. Nous avons généralisé l'utilisation de ce graphe bande aux implantations séquentielles et parallèles de notre outil de partitionnement Scotch.<br /><br />Grâce à la présence du graphe bande, nous avons proposé une utilisation nouvelle des algorithmes génétiques dans le cadre de<br />l'expansion en les utilisant comme heuristiques parallèles de raffinement de la partition.

Les limites d'échelle de grands arbres aléatoires jouent un rôle central dans cette thèse.Nous nous intéressons plus spécifiquement au comportement asymptotique de plusieurs fonctions codant des arbres de Galton-Watson conditionnés. Nous envisageons plusieurs types de conditionnements faisant intervenir différentes quantités telles que le nombre total de sommets ou le nombre total de feuilles, avec des lois de reproductions différentes.Lorsque la loi de reproduction est critique et appartient au domaine d'attraction d'uneloi stable, un phénomène d'universalité se produit : ces arbres ressemblent à un même arbre aléatoire continu, l'arbre de Lévy stable. En revanche, lorsque la criticalité est brisée, la communauté de physique théorique a remarqué que des phénomènes de condensation peuvent survenir, ce qui signifie qu'avec grande probabilité, un sommet de l'arbre a un degré macroscopique comparable à la taille totale de l'arbre. Une partie de cette thèse consiste à mieux comprendre ce phénomène de condensation. Finalement, nous étudions des configurations non croisées aléatoires, obtenues à partir d'un polygône régulier en traçant des diagonales qui ne s'intersectent pas intérieurement, et remarquons qu'elles sont étroitement reliées à des arbres de Galton-Watson conditionnés à avoir un nombre de feuilles fixé. En particulier, ce lien jette un nouveau pont entre les dissections uniformes et les arbres de Galton-Watson, ce qui permet d'obtenir d'intéressantes conséquences de nature combinatoire.

The major purpose of this study was to investigate the effects of using animal organ dissection in general, and its use specifically in problem-solving as a teaching strategy in Grade 11 Life Sciences education. A multiple methods research design was used for this study. The data collection methods for the quantitative approach were the pre-test, post-test and a questionnaire. The pre-test and post-test had predominantly problem-solving questions. The questionnaire and the tests were administered to 224 learners from four Pretoria East secondary schools from different environments. The data collection methods for the qualitative approach were the interviews with the Grade 11 Life Sciences teachers of the selected schools, lesson observations and relevant document analysis. The interviews were conducted with six Grade 11 Life Sciences teachers teaching at the four selected schools. Findings from both the quantitative and the qualitative approaches were integrated to give an in-depth understanding of the study. The findings show that there were significant differences between the means of the pre-test and the post-test for the total for the whole group of 224 learners. The variables in which the tests were categorised were the rote learning, problem-solving and three learning outcomes of the National Curriculum Statement (NCS). The way in which the learners answered the questions in terms of terminology they used, the confidence they displayed, the level of answering and the explanations they gave when they wrote the post-test were significantly different from when they wrote the pre-test. The significant differences between the means of the pre-test and the post-test may possibly have been due to the intervention. This showed the effectiveness of the intervention which was animal organ dissection in problem-solving. The study also showed that most teachers are not well-acquainted with problem-solving strategies which made it challenging for them to use animal organ dissections to develop problem-solving skills in learners. The attitudes of the teachers and learners towards animal organ dissection and its use in problem-solving as a teaching strategy were predominantly positive with less than a quarter of the whole group being negative due to a variety of reasons which include: moral values, religion, culture, blood phobia, squeamishness and being vegetarian. The majority of learners acknowledged the importance of animal organ dissections in developing skills like investigative, dissecting and problem-solving skills. This acknowledgement resulted in them being positive towards the use of animal organ dissections in problem-solving. One can conclude that animal organ dissections can be used in problem-solving as a teaching strategy in Life Sciences education. The level of learner engagement with animal organ dissections can determine the level of development of problem-solving skills as was evidenced by the differences between the mean scores of the four schools. The study recommended that the teachers should be encouraged to use animal organ dissections more frequently where it is applicable to develop problem-solving skills in learners and not merely let the learners cut, draw and label the organ. Teachers should also focus on problem-solving in general and develop this as a prime strategy. All activities should be prepared by the teacher and implemented in class to encourage and develop problem-solving skills. / Thesis (PhD)--University of Pretoria, 2013. / gm2014 / Science, Mathematics and Technology Education / restricted

Materials which exhibit non-linear mechanical behaviors under large deformations are generally classified as “soft matter”. Elastomers represent an important class of soft materials which have wide commercial applications and isotropic non-linear behavior. In contrast, biological materials have anisotropic responses due to their heterogeneous and composite architectures. The underlying microstructure determines the arterial macroscopic behavior and is represented through constitutive models to describe the stress-strain relationships. Mechanical characterization and development of constitutive models that describe these non-linear and anisotropic properties are essential to our understanding of the structure-property relationships in these materials. In this study, we use two model systems to link the local microstructure to the overall macroscopic behaviors of soft matter. First, we delineate the roles of individual network topological factors in determining the overall macroscopic behavior of isotropic silicone elastomers using specimens fabricated with differential amounts of crosslinking. We performed mechanical experiments, within a theoretically motivated continuum mechanical framework, using a custom made planar biaxial testing instrument. These experiments demonstrate the contributions of physical entanglements and chemical crosslinks to the overall mechanical properties of silicone elastomers. Further, we show that the slip-link form of strain energy function is better suited to describe the material properties in the low to moderate regions of the stress-strain behavior. However, this model does not predict the stiffening response of elastomers at higher deformations, which is better captured using the Arruda-Boyce form of strain energy function. To explore the effects of individual topological factors on the overall network properties, we performed swelling experiments of silicone specimens in xylene and quantified variations in the polymer-solvent interaction parameter, χ, given by the Frenkel-Flory-Rehner (FFR) model. Further, we characterized the viscoelastic properties using dynamic mechanical analysis. Our results show that χ is not a constant, as assumed in the FFR model, but bears a linear relation to the equilibrium polymer volume fraction. To characterize the contribution of trapped entanglements to the overall mechanical behaviors, we use scaling laws in polymer physics and investigate the dependence of equilibrium volume fraction and experimentally obtained elastic moduli. Further, dynamic mechanical analysis demonstrated an increase in complex modulus with increase in the cross linking density. Finally, we examined variations in the uniaxial and the dynamical mechanical properties of silicone elastomers with storage time. Our results show that the time dependent increase in the modulus correlated with the formation of slip-links in the samples aged for a significantly long time in air. Together, these comprehensive studies show the importance of individual network features which affect the overall macroscopic properties of elastomers. Second, we use a multilayered and composite arterial model system to explore the passive material properties of arteries due to anisotropic layouts of extracellular matrix proteins, collagen and elastin. We characterized the mechanical properties of diseased human ascending thoracic aortic dissected (TAD) tissues, obtained from consenting patients undergoing emergency surgical repair to replace the diseased region, using multiple biaxial tests. We fit these results to micro structurally motivated Holzapfel-Gasser-Ogden model which is frequently used in the arterial mechanics literature. Our results show a higher stiffness for TAD tissues as compared to control aorta, without the presence of atherosclerotic plaques or other arterial disease. To study the directional variation in the mechanical properties of TAD tissues, we compared the stiffness in circumferential longitudinal directions at high and low stress region of equibiaxial experimental data. We observed no differences in the stiffness of TAD tissues in the circumferential and longitudinal directions. Further, we do not see any directional variations in the ultimate tensile stress, maximum extensibility, and modulus calculated in the low stretch region of uniaxial stress-strain response in TAD tissues. Histological analysis of TAD tissues showed a decrease in elastin content and an increase in collagen content as compared to control tissues. Higher TAD tissue stiffness also correlated with reduced elastin content in the arterial walls. To investigate the strain rate dependence of measured mechanical properties we use high testing rates of 1mm/sec to show that the TAD tissues have higher stiffness in the circumferential direction as compared to longitudinal direction. Finally, we used peel experiments to quantify the rupture potential of aortic dissected tissues. Our results show that TAD tissues have reduced delamination strength between layers as compared to control aortic tissues. To the best of our knowledge, no previous study has reported the mechanical property of human TAD tissues and these are the only biomechanical results on TAD tissues reported in specimens from South Asian patients. We hope that such studies will be useful for researchers who rely on microstructure based constitutive models to accurately describe the mechanical environment of cells which are important in the remodeling of tissues and in numerical models to assess mechanical criteria which may lead to the growth or dissection of arterial tissues.