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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
91

Microfluidic system with open loop control for rapid infrared reverse transcription quantitative PCR (RT-qPCR)

Saunders, Daniel Curtis 05 July 2012 (has links)
Microfluidic techniques have allowed for fast, sensitive, and low cost applications of the Polymerase Chain Reaction (PCR) through the use of small reaction volumes, rapid amplification speeds, and the on-chip integration of upstream and downstream sample handling processes including purification and electrophoretic separation functionality. While such systems are capable of measuring the expression levels of thousands of genes simultaneously, or in hundreds of cells, or with sample-in and answer-out capability, none of these systems are easily scalable in the time domain. Because of this, the field of gene expression measurement has yet to fully utilize the advantages of microfluidic PCR in developing systems to measure changes in gene expression in increments of hours rather than days. In this project, we developed a microfluidic RT-qPCR system that utilizes infrared heating and open-loop control to reliably reverse transcribe, amplify, and detect samples in a single 1 μl polymer chip. Optimized power profiles were created that allow for fast heating and cooling rates while minimizing undershoot and overshoot from the desired hold temperatures. By utilizing repeatable microfluidic chip manufacturing techniques, and by controlling the environment around the chip, the same open loop program can repeatedly amplify multiple samples without any need for temperature feedback or recalibration between runs. Furthermore, the system was designed to operate on top of a fluorescence microscope to enable real-time fluorescence detection and quantification of starting copy number. By eliminating complicated setup procedures and calibration runs, this system increases the practicality of measuring gene expression at a high temporal frequency.
92

Modeling, simulation and performance optimization of wideband semiconductor optical amplifiers

Park, Jongwoon. Huang, W.P. January 2004 (has links)
Thesis (Ph.D.)--McMaster University, 2005. / Supervisor: W.-P. Huang Includes bibliographical references (leaves 124-136).
93

Direct and inverse methods for waveguides and scattering problems in the time domain /

Abenius, Erik, January 2005 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 5 uppsatser.
94

Local mesh refinement algorithms for enhanced modeling capabilities in the FDTD method /

Chavannes, Nicolas Pierre. January 2002 (has links)
Diss. ETH No. 14577. Eidgenöss. Techn. Hochsch., Diss.--Zürich, 2002.
95

Development of an accelerated finite-difference time-domain solver using modern graphics processors

Price, Daniel Kenneth. January 2009 (has links)
Thesis (M.E.E.)--University of Delaware, 2007. / Principal faculty advisor: Dennis W. Prather, Dept. of Electrical & Computer Engineering. Includes bibliographical references.
96

Analysis and design of switching DC/DC converters /

Plesnik, Martin, January 1900 (has links)
Thesis (M.App.Sc.) - Carleton University, 2002. / Includes bibliographical references (p. 175-182). Also available in electronic format on the Internet.
97

Accurate macromodeling of high-speed interconnects characterized by time-domain measurements /

Harb, Kamal, January 1900 (has links)
Thesis (M.App.Sc.) - Carleton University, 2003. / Includes bibliographical references (p. 121-128). Also available in electronic format on the Internet.
98

A synchronous filter for gear vibration monitoring using computational intelligence

Mdlazi, Lungile Mndileki Zanoxolo. January 2005 (has links)
Thesis (M. Eng.(Mechanical Engineering))--University of Pretoria, 2004. / Includes bibliographical references.
99

Novel adaptive time-domain techniques for the modeling and design of complex RF and wireless structures

Bushyager, Nathan Adam. January 2004 (has links)
Thesis (Ph. D.)--Electrical and Computer Engineering, Georgia Institute of Technology, 2005. / Tentzeris, Manos, Committee Chair ; Laskar, Joy, Committee Member ; Peterson, Andrew, Committee Member ; Papapolymerou, Ioannis, Committee Member ; Sotiropoulos, Fotis, Committee Member. Vita. Includes bibliographical references.
100

Weakly non-local arbitrarily-shaped absorbing boundary conditions for acoustics and elastodynamics theory and numerical experiments

Lee, Sanghoon, Kallivokas, Loukas F., January 2004 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2004. / Supervisor: Loukas F. Kallivokas. Vita. Includes bibliographical references.

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