Made available in DSpace on 2016-04-28T20:15:34Z (GMT). No. of bitstreams: 1 2006-Raquel Saucier Gomes.pdf: 2225218 bytes, checksum: 68d6b849a23bb4479d6785b36389a032 (MD5) Previous issue date: 2006-03-09 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The aim of the present work was to identify the occurrence of natural Cryptosporidium infection in domestic birds, commercialized in two public markets, in the city of Rio de Janeiro. 180 birds were acquired, being 60 ducks (Anas platyrhynchos), 60 chicks (Gallus gallus) and 60 quails (Coturnix japonica). The birds were housed in individual cages at the Protozoology laboratory of the Federal Rural University of Rio de Janeiro. As diagnostic method for the occurrence and infection dynamics was used de centrifuge - fluctuation technique. Measurements of the major and minor oocyst diameters were realized and compared between the origins of the birds. From the market of Madureira, a total of 29 (96,7%) ducks, 20 (66,66%) quails and 30 (100%) chicks were infected with Cryptosporidum sp. At the market of Campo Grande 17 (56, 7%) ducks, 13 (43, 33%) quails and 22 (73, 33%) chicks were infected. Statistically significant differences between the origin of the birds and infection rate were encountered for the ducks and the chicks, but not for the quails. The pre-patent period of the infection varied between 2 and 8 days, and the patent period varied between 3-46 days. Ducks present major resistance to the infection, but once established they present a longer patent period, with higher oocyst shedding than do quails and chicks. Observing the presented results can be concluded that the natural infection with Cryptosporidium is frequent in the studied domestic birds commercialized in public markets. Ducks, chicks and quails may play an important part in the dissemination of cryptosporidiosis in public markets, possibly representing a risk for human infection in persons that work or does frequent the markets. More studies should be undertaken to clarify about the risk factors associated with cryptosporidiosis and molecular studies for the characterization of the Cryptosporidium species involved. / O presente trabalho teve como objetivo identificar a ocorr?ncia da infec??o pelo protozo?rio do g?nero Cryptosporidium em aves dom?sticas comercializadas em dois mercados municipais situados no munic?pio do Rio de Janeiro - RJ, e associar poss?veis fatores de risco relacionados ? infec??o. No total foram adquiridas 180 aves, sendo 60 patos (Anas platyrhynchos), 60 pintos (Gallus gallus) e 60 codornas (Coturnix japonica). As aves foram encaminhadas ao laborat?rio de Protozoologia da Universidade Federal Rural do Rio de Janeiro e acondicionadas em gaiolas individuais. A ocorr?ncia e din?mica de Cryptosporidium sp. nessas aves foram estudadas atrav?s da t?cnica de centr?fugo-flutua??o. Mensura??es do maior di?metro e menor di?metro dos oocistos foram realizadas e comparadas entre as aves dos mercados. No mercado de Madureira um total de 29 (96,7%) patos, 20 codornas (66,66%) e 30 pintos (100%) apresentaram infec??o por Cryptosporidum sp. e no mercado de Campo Grande 17 (56,7%) patos, 13 codornas (43,33%) e 22 (73,33%) pintos estavam infectados. Diferen?as estatisticamente significativas foram encontradas para a ocorr?ncia da infec??o entre os pintos e de patos dos dois mercados, por?m n?o para codornas. O per?odo pr?-patente da infec??o variou entre 2 e 8 dias e per?odo patente entre 3 a 46 dias. Patos possuem uma maior resist?ncia ? aquisi??o da infec??o por Cryptosporidium sp. que pintos e codornas, por?m uma vez estabelecida a infec??o, esta possui maior per?odo patente, com altas concentra??es de oocistos nas fezes, se comparado a pintos e codornas. Pode-se concluir que a infec??o natural por Cryptoporidium ? frequente nas aves estudadas. Patos, pintos e codornas podem ser disseminadores de criptosporidiose em mercados municipais, cria??es dom?sticas e/ ou de subsist?ncia dentro do munic?pio do Rio de janeiro, assim como podem constituir risco para infec??o em humanos, como crian?as, tratadores, comerciantes e frequentadores do local. Mais estudos ser?o feitos para a elucida??o dos fatores de risco associados ? infec??o por Cryptosporidium sp., assim como estudos de caracteriza??o molecular de isolados provenientes dos mercado municipias no Rio de Janeiro.