Evapotranspiration rates (ET) and consumptive water use (CWU) were measured for three desert turfgrasses using weighing lysimeters with a calcined clay growth medium. Water use rates were compared over a two-year study for 'Sea Isle I' seashore paspalum (Paspalum vaginatum) and A138 desert saltgrass (Distichlis spicata), along with 'Tifway' bermudagrass (Cynodon dactylon x Cynodon transvaalensis). Saltgrass showed elevated leaf canopy temperatures for extended periods before exhibiting visible wilt symptoms while 'Tifway' bermudagrass and seashore paspalum wilted readily, even at relatively high soil water contents. Saltgrass transpired for 11.9 d on average before drought stress was observed. Seashore paspalum maintained leaf turgor, on average 5.6 d without irrigation, whereas 'Tifway' bermudagrass averaged 4.0 d before requiring irrigation. The two-year average Kc values for three grasses before self-imposed drought was expressed by visible leaf wilting were 0.85, 0.79, and 0.74, for A138 saltgrass, 'Sea Isle I' seashore paspalum, and 'Tifway' bermudagrass, respectively.

In this research, I describe the use of false rings to reconstruct local histories of seasonal drought in riparian ecosystems in semiarid regions. In tree-ring analysis, false rings are boundary-like features often formed as a response to drought within the growing season. Drought can be a common feature in hydrologic regimes of dryland rivers but in recent decades drought has been intensifying due to climate change and increasing water use by cities, agriculture and industry. Identifying when and where water availability has decreased along the river course is critical for understanding, and therefore managing, these generally endangered ecosystems. The higher density of trees compared to instrumental data make them ideal candidates for reconstructing site-specific drought patterns.The first part of this dissertation is an observational study conducted on the San Pedro River in southeastern Arizona during 2002. I used dendrometer data and local hydrological data to show that a period of negligible radial growth in cottonwood during the middle of the growing season coincided with a channel drying event. Tree-ring core samples confirmed that false-rings had formed in each of the instrumented trees. The second part of this dissertation is an experimental study designed to evaluate the effect of different levels of water stress on false-ring formation in cottonwood and willow. I showed that experimental decreases in water availability for periods as short as ten days were enough to induce false-ring formation in willow. Longer periods of reduced water availability were generally required to induce false-ring formation in cottonwood. In the final part of this dissertation, I reconstructed false-ring occurrence in Fremont cottonwoods at three sites along the San Pedro River. I infer from false-ring frequencies that the severity of summer drought has been increasing over the last four to six decades but that the drought severity varies along a hydrological gradient. Overall, the findings in this body of research confirm that false rings in riparian tree species can be used as indicators of seasonal drought and underscore the importance of identifying site-specific responses to reduced water availability along the riparian corridor.

Changing hydroclimatic conditions are the primary source of risk to hydroelectric power generation. The research described in this dissertation investigates hydrological and drought variability in the Winnipeg River basin, Canada, during the last several hundred years using instrumental hydroclimate data and paleoclimatic records derived from tree rings. The basin drains parts of northwestern Ontario, northern Minnesota and southeastern Manitoba, and is the most important component of the hydrological system used to generate power in Manitoba. Extreme low annual flows in the Winnipeg River are associated with enhanced meridional flow across western Canada during summer and autumn, which suppresses precipitation over the watershed and reduces runoff from spring snowmelt. In contrast to the declining flows observed for other regional rivers, mean annual discharge in the Winnipeg River basin has increased substantially since the early 1920s. For a longer perspective, fifty-four ringwidth chronologies (mainly Pinus resinosa and P. strobus) were used to assess changes in summer climate in the Winnipeg River region since AD 1783. Tree growth in this region is significantly, but weakly, correlated with both temperature and precipitation during summer. Synthetic tree-ring records produced by the Vaganov-Shashkin model of tree-ring formation are consistent with these relationships with climate, and suggest that the primary factor limiting tree growth switches from temperature to moisture in mid-summer. The Winnipeg River tree-ring record indicates that summer droughts were more persistent in the 19th and late 18th century than during the last 100 years, but there is no evidence that drought was more extreme prior to the onset of direct monitoring.This dissertation also examines past changes in summer drought over the broader region using 138 ringwidth records from the Canadian Prairies provinces and adjacent areas. Regional ringwidth signals are primarily related to summer moisture and drought conditions. These summer-sensitive records are not linearly related to major modes of climate variability, including ENSO and the PDO, which mainly affect the climate of western Canada during winter. Extended drought records inferred from regional tree-ring series indicate that drought on the Canadian Prairies has exhibited considerable spatial heterogeneity over the last several centuries.

A tree-ring width chronology from Scots pine (Pinus sylvestris L.) was constructed from a xeric site in Stockholm to investigate the relationships between climate and tree growth and to reconstruct past moisture variability. The measure of moisture conditions employed here is a self-calibrating Palmer Drought Severity Index (PDSI). The index is derived from temperature, precipitation, and available water capacity of the soil, and assesses the intensity and duration of drought. It is widely used in tree-ring based climate reconstructions, a method which has never before been tested in the Nordic countries. The comparison of the Stockholm tree-ring chronology with monthly temperature and precipitation data from a nearby meteorological station shows that tree growth is reduced by high summer temperatures, whereas high precipitation at the beginning of the growing season favours growth. The comparison with a PDSI calculated from this meteorological data shows that negative PDSI values are associated with narrow rings. Although tree growth in the humid climate of central Sweden is generally not limited by precipitation, the trees sampled for this study prove to be sensitive to changes in water supply. Their rings thus provide a record of past moisture variability and enable the reconstruction of precipitation and drought. The transfer function models for the reconstructions are calibrated using linear regression. A detailed verification of the results using the more than 200-year long meteorological record from Stockholm affirms the good model performance. May–June precipitation sums and the July PDSI could be reconstructed back to 1625. The Palmer Drought Severity Index is found to be a useful tool in a tree-ring based reconstruction of past moisture variability, approximating the fraction of rainfall which is actually available to the tree, by including soil moisture storage, runoff, and the influence of temperature on evapotranspiration. It cannot completely account for the combined temperature and precipitation forcing of tree growth, and the use of the index does not improve the reconstruction compared to using precipitation alone. However, a reconstruction of both precipitation and the PDSI is possible when selecting an adequate sample site.

This research examines the potential to use passive microwave remote sensing for measuring soil moisture extremes that impact agricultural areas in Canada. A validation was made of three passive microwave remote sensing soil moisture data sets, with weekly averaged values from the Land Parameter Retrieval Model (LPRM) applied to AMSR-E C/X-Band data providing the most accurate results (root mean squared error of 5 to 10%). A further evaluation of this data set against a spatially distributed in situ soil moisture network in Alberta suggests that this data set may be less accurate in regions where dense vegetation or open water is present, particularly on the northern edges of the Canadian agricultural extent. A method to derive soil moisture anomalies was developed that uses homogenous regions to spatially aggregate soil moisture statistics to compensate for a short satellite data record. It was found that these anomalies can be estimated with errors of less than 5% when these regions are 15 pixels or more over a seven year time period. Surface soil moisture anomalies from LPRM showed weak but significant relationships to precipitation based drought indices, suggesting promise for using these anomalies for wider soil moisture extremes monitoring. Soil moisture anomalies from CLASS and in situ networks showed inconsistencies with LPRM anomalies in how they capture soil moisture conditions that are relevant to agricultural yield.. These data sets overall show that this approach to quantifying extremes has potential, but improvement to soil moisture retrieval from LPRM and CLASS, and an integration of the information they provide are needed to optimize these data sets for agricultural monitoring. / National Science and Engineering Research Council, Agriculture and Agri-Food Canada, Canadian Space Agency

No description available.

Plants have evolved numerous strategies for the adaptation to drought. Although many investigations reported on the potential value of proline accumulation during environmental stress, it is still unknown whether or not a constitutive higher level of proline accumulation enhances plant tolerance. Thus, it was investigated if underproduction and overproduction of proline will influence the susceptibility to drought stress in soybean plants. This was made possible with the transformation of soybean plants with an L-Δ¹-pyrroline-5-carboxylate reductase (P5CR) gene. First, an Agrobacterium-mediated vacuum infiltration transformation system, using partially germinating Carnia 2233 soybean seed, was established through the assessment of several conditions that can affect transformation efficiency with the use of β-glucuronidase reporter genes. Transformation was confirmed with PCR and Southern blot analysis and results indicated that stable transgenic soybean plants were obtained within one generation with a transformation rate of± 30%. This technique was used in the transformation of Carnia 2233 soybean seed with the P5CR gene in the antisense orientation under the control of an inducible heat shock gene promoter (IHSP). It was confirmed that the P5CR-IHSP gene construct was integrated into the soybean cells and was conserved over three generations. Physiological screening of the antisense P5CR transgenic plants in the greenhouse proved that, with activation of the promoter, an under-expression of the P5CR gene and subsequent inhibition of the accumulation of proline were experienced during drought and osmotic stress. The decline of the viability of the transgenics with prolonged drought stress, as monitored with a woodenbox screening test, is an indication that proline is needed for survival of soybean plants under drought stress conditions. The transgenic plants demonstrated a sensitive reaction in contrast to the control plants that displayed a tolerant reaction to osmotic stress in a TTC assay. The underexpression of the P5CR gene resulted in a decline protein synthesis due to proline shortage as was observed with the evaluation of the efficiency of protein synthesis. All these results suggest that a decrease in the proline level due to the antisense P5CR gene, yielded plants that are more osmotic and drought stress sensitive. Subsequently, the soybean cultivar Ibis was successfully transformed with the P5CR-IHSP construct in the sense and antisense directions in order to test the reproducibility of the transformation process and to assessed the link between the biochemical traits involved in the drought stress mechanism. Three different experiments were conducted: a mild heat and drought stress on "To" transgenic plants exploring changes in chlorophyll fluorescence transients, a mild heat stress on "T1" transgenic plants comparing proline accumulation and chlorophyll fluorescence transients and a severe drought and heat stress on the "T1" transgenic plants comparing proline accumulation NADP⁺synthesis and chlorophyll fluorescence transients. Chlorophyll fluorescence transients were successfully used as a screening method for transgenic soybean plants during this study. The sense transgenics responded to the mild stresses with a significant decrease in their electron transport, trapping and absorption compared to the antisense plants that displayed significant increases in electron transport and trapping. During the severe stress, the antisense transgenics experienced total photoinhibition indicated by the enormous loss of electron transport but the sense plants had the ability to overcome the stress as is revealed in the increase in the electron transport. It was demonstrated that although proline accumulation yielded no significant differences during the mild heat stress, the sense plants accumulated substantially more proline than the control and antisense plants during the severe heat and drought stress. It was demonstrated that proline plays an important role in the plant's response to a drought stress as well as in the recovery phase after drought, as the sense plants also had the ability to reduce the accumulated proline during the recovery period in contrast to the antisense transgenics that experienced protein degradation. The transgenics responded to a period of heat and drought stress with a reduction in NADP⁺ levels in the antisense plants and increasing levels in the sense plants. The sense plants were able to fully recover after the stress period, thus adaptation to drought may depend on different mechanisms, including the capacity to maintain high levels of proline and to regenerate them through the "reduction" of NADP⁺. It was possible to alter the drought tolerance of Ibis by transformation with antisense and sense P5CR gene constructs, which resulted in respectively more sensitive and more tolerant Ibis plants. It can be concluded that over-expression of P5CR during a drought stress resulted in higher proline levels, better photosynthetic efficiency, higher NADP⁺ production and thus a more drought tolerant plant. This study gave additional proof that a constitutively higher level of proline accumulation enhances drought tolerance in soybean. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2000.

There is a great deal of work currently being done in the field of desiccation tolerance. Generally workers studying desiccation-tolerant plant tissues have concentrated on the mechanisms of desiccation tolerance without concomitant studies on why most plants cannot survive desiccation. The present study considers both a desiccation-tolerant plant as well as a range of desiccation-sensitive plants. The work incorporates physiological, biophysical, biochemical and ultrastructural studies in an attempt to get a holistic picture of vegetative material as it dries and then rehydrates. The plant species used in this study are: Craterostigma nanum, a so-called resurrection plant; Garcinia livingstonei, a drought-tolerant small tree; Isoglossa woodii, an understorey shrub which shows a remarkable ability to recover from wilting; Pisum sativum seedlings, which have a very high water content at full turgor; and finally, Adiantum raddianum, the maiden hair fern, which wilts very quickly and does not recover from wilting. The desiccation-tolerant plant, C. nanum, had an unusual pressure-volume (PV) curve which indicated that while large volume changes were taking place there was little concomitant change in pressure or water potential. The unusual nature of this PV curve made it difficult to assess the relative water content (RWC) at which turgor was lost. The desiccation-sensitive plants exhibited standard curvi-linear PV curves. The amount of nonfreezable water in the five species was studied and found to show no correlation with the ability to withstand dehydration or with the lethal water content. There were no differences in the melting enthalpy of tissue water between the tolerant and most of the sensitive plants. Isoglossa woodii had a lower melting enthalpy than the tolerant and the other sensitive species. Survival studies showed that the desiccation-sensitive plants all had similar lethal RWCs. The tolerant plant survived dehydration to as low as 1% RWC, recovering on rehydration within 24 hours. Membrane leakage studies showed that the sensitive plants all exhibited membrane damage at different absolute water contents, but very similar RWCs and water potentials. The increase in leakage corresponded to the lethal RWC for all the sensitive species. The desiccation-tolerant plant recovered from dehydration to very low water contents and did not show an increase in membrane leakage if prior rehydration had taken place. Without prior rehydration this tolerant plant exhibited an increase in leakage at similar RWCs and water potentials to that of the sensitive species. There did not appear to be much difference in the RWC at which damage to membranes occurred whether the material was dried rapidly or slowly. Respiration and chlorophyll fluorescence were studied to determine what effect drying and rehydration have on the electron transport· processes of the leaf. The chlorophyll fluorescence studies gave an indication of damage to the photosynthetic apparatus. Both qualitative changes as well as quantitative changes in fluorescence parameters were assessed. Characteristics like quantum efficiency (Fv/Fm)remained fairly constant for a wide range of RWCs until a critical RWC was reached where there was a sharp decline in Fv/Fm. Upon rehydration, C. nanum recovered to pre-stress levels, I. woodii showed no recovery and no further damage on rehydration, whilst the other species exhibited even more damage on rehydration than they had on dehydration. Respiration remained fairly constant or increased slightly during drying until a critical RWC was reached at which it suddenly declined. The RWC at which this decline occurred ranged from 15% and 20% in P. sativum and C. nanum respectively, to 50% for G. livingstonei. On rehydration respiration exceeded the levels measured in dehydrated material for the sensitive species. Unsuccessful attempts were made to fix material anhydrously for ultrastructural studies so standard fIxation was used. The ultrastructural studies revealed that changes had occurred in the ultrastructure of leaves of the sensitive species dried to 30% RWC particularly in A. raddianum and P. sativum. Drying to 5% RWC revealed extensive ultrastructural degradation which was worsened on rehydration in the sensitive species. The tolerant species showed ultrastructural changes on drying but these were not as severe as occurred in the sensitive species. The cell walls of the tolerant species folded in on drying. This folding was possibly responsible for the unusual PV curves found in this species. At 5% RWC the cells were closely packed and very irregular in shape. The cell contents were clearly resolved and evenly spread throughout the cell. The large central vacuole appeared to have subdivided into a number of smaller vacuoles. On rehydration the cells regained their shape and the cell contents had moved towards the periphery as the large central vacuole was reformed. Beading of membranes, which was common in the sensitive species, was not found in the tolerant species suggesting that membrane damage was not as severe in the tolerant species. Western Blot analysis of the proteins present during drying was performed to determine whether a class of desiccation-induced proteins, called dehydrins, were present. These proteins have been suggested to play a protective role in desiccation-tolerant tissue. It was found that C. nanum did, in fact, possess dehydrins, but so did P. sativum. The other three sensitive species did not show any appreciable levels of dehydrin proteins. The presence of dehydrins alone is, therefore, not sufficient to confer desiccation tolerance. While physiologically the damage occurring in the sensitive plants was similar to that of the tolerant plant, at an ultrastructural level the damage appeared less in the tolerant plant. On rehydration from low RWCs damage appeared to become exacerbated in the sensitive plants. This was in contrast to the tolerant plant where damage was apparently repaired. There appears, therefore, to be a combination of protection and repair mechanisms responsible for the ability of C. nanum to tolerate desiccation. The lethal RWC of the sensitive species was higher than that at which protective mechanisms, such as water replacement, might come into play. So it is not just the possible ability to replace tightly bound water that set the tolerant plant aside. It must also have mechanisms to tolerate damage at the higher RWCs which were damaging and lethal to the sensitive plants. The lethal damage to sensitive species appeared to be related to a critical volume, thus it is concluded that the tolerant plant had the ability to tolerate or avoid this mechanical damage during drying as well as the ability to remain viable in the dry state. It is hypothesised that the ability of the walls to fold in and the unusual nature of the PV curve may provide some answers to the enigma of desiccation tolerance. / Thesis (Ph.D.)-University of Natal, 1995.

An infusion technique was used with an aqueous radiocalcium ( 45CaCl2) solution during the seed-filling stage of well-watered and moisture-stressed soybean in a greenhouse. The kinetics of infusion volume showed a quadratic reduction in absorption which approached zero on the sixth day for non-irrigated plants. The concentration of 45Ca increased quadratically from the point of injection towards the apex independent of both water status and plant parts. The difference in concentration of 45Ca between irrigated and non-irrigated plants was significant (P < 0.05) and concentrations attained the maximum values at the sixth node from the plant base. Seeds contained considerably less 45Ca than either stem or leaves. / The effects of a long (LD, 16h) and a short (SD, 12h) photoperiod with two water stress levels (SL) of stress (ST) and no stress (NS) on the distribution of 45Ca in plant organs (PO) of leaves, petioles, and stem at different node number (NN) of soybean were studied during the seed-filling stage. The univariate and Manova analyses showed the main effects of photoperiod (PP), SL, and PO to be highly significant (P < 0.001) on Ca distribution. The long PP increased Ca concentration in top leaves compared with the short PP regardless of SL. Water stress significantly (P < 0.001) modified the Ca distribution and reduced its concentration in PO within NN irrespective of the photoperiod (a measure of light stress). A possible mechanism for the regulation of Ca distribution is discussed in terms of nitrate reduction. / Ca uptake was also studied by immersing the central tip of a trifoliate leaf in various concentrations of 45CaCl2 solutions and drought conditions during the seed-filling period of soybean. The beta-ray gauging and the diurnal leaf temperature variation showed similar characteristics for leaf water status. The activities of 45Ca were significantly higher (P < 0.0001) at 5, 10, 20, and 30 mM concentrations for water-stressed and non-stressed leaves compared with the control. 45Ca activities at 5, 10, and 20 mM Ca concentrations between stressed and non-stressed leaves were not significant, but the difference in their mean values at 30 mM Ca concentration was significant (P = 0.0159). The relationship between 45Ca uptake and Ca concentration was parabolic for both stressed (R 2 = 0.77) and non-stressed (R2 = 0.81) leaves. Autoradiograms indicated Ca movement through the mid-rib and veins of the tip-immersed trifoliate leaf but showed no activity in other plant parts. An activity gradient developed between seeds when a pod-tip was immersed in the radioactive solution. Solutions of ruthenium red (RR, 0.01 mM), Ethylene Glycol-bis-(beta-aminoethyl ether)- N,N,N,N-Tetraacetic Acid (EGTA, 0.1 mM), calcium (Ca, 1 mM), and double distilled water (control) were fed through a bottom branch of soybean with (ST) and without (NS) water stress. The volume absorptions and transpiration rates were significantly higher for NS than ST plants and decreased almost linearly with time for all treatments. The transpiration rates of Ca-feeding ST plants and the control overlapped while the NS plants approached the same rate of transpiration by the third week. Ca was implicated in stomatal closure for the reduction in the transpiration rates. The relative amounts of chlorophyll decreased with time but chlorophyll was least affected for Ca-absorbing plants for both ST and NS plants. The use of RR (Ca transport blocker), and EGTA (Ca chelator) indicated the role of intracellular Ca conce

The Bromeliaceae family utilizes a wide range of adaptations to inhabit a variety of environments including dry ones. Many attribute the large adaptive radiation of Bromeliaceae throughout the Neotropics to three main features: absorptive trichomes, tank reservoirs, and CAM photosynthesis. Based on leaf morphology and arrangement, root type, and nutrient acquisition, Pittendrigh (1948) conservatively separated bromeliads into four main classes. These four main classes are designated Type I bromeliads, Type II bromeliads, Type III bromeliads and Type IV bromeliads. We used RNA-sequencing of leaf mRNA to investigate similarities and differences in gene expression which can be related back to the four distinct leaf morphologies in the Bromeliaceae family. We found several transcripts relating to the presence of a tank and absorptive trichomes. In addition, we found evidence of varying forms of carbohydrate synthesis for carbon storage during CAM photosynthesis. Lastly, transcriptomics differences indicate different drought survival strategies, with the most extreme differences occurring between Aechmea nudicaulis and Tillandsia gardneri. This study identified transcripts related to the morphological gradient and highlighted how each ecological type has a particular set of adaptations and strategies for survive in a particular regime.