Heterotrophic protists, consisting largely of the Cercozoa, Amoebozoa, Ciliophora, Discoba and some Stramenopiles, are a poorly characterized component of life on Earth. They play an important ecological role in soil communities and provide key insights into the nature of one of life’s most enigmatic evolutionary transitions: the development of the complex unicell. Soil ecosystems are crucial to the functioning of global biogeochemical cycles (e.g. carbon and nitrogen) but are at risk of drastic change from anthropogenic climate change. Heterotrophic protists are the primary regulators of bacterial diversity in soils and as such play integral roles in biogeochemical cycling, nutrient mobilization, and trophic cascades in food webs under stress. Understanding the nature of these changes requires examining the rates, diversity, and resiliency of interactions that occur between soil organisms. However, soils are the most taxonomically diverse ecosystems on Earth and disentangling the complexities of dynamic and varied biotic interactions in them requires a unique model system. The McMurdo Dry Valleys of Antarctica, one of the harshest terrestrial environments on Earth, serve as a model soil ecosystem owing to their highly reduced biological diversity. Exploring the functioning of heterotrophic protists in these valleys provides a way to test the applicability of this model system to other soil food webs. However, very little is known about their taxonomic diversity, which is a strong predictor of function. Therefore, I reviewed the Antarctic literature to compile a checklist of all known terrestrial heterotrophic protists in Antarctica. I found significant geographical, methodological, and taxonomic biases and outlined how to address these in future research programs. I also conducted a molecular survey of whole soil communities using 18 shotgun metagenomes representing major landscape features of the McMurdo Dry Valleys. The results revealed the dominance of Cercozoa and point to an Antarctic heterotrophic protist soil community that is taxonomically diverse and reflects the structure and composition of communities at lower latitudes. To investigate whether biotic interactions or abiotic factors were a larger driver for Antarctic heterotrophic protists, I conducted variation partitioning using environmental data (e.g. moisture, pH and electrical conductivity). Biotic variables were more significant and accounted for more of the variation than environmental variables. Taken together, it is clear that heterotrophic protists play key ecological roles in this ecosystem. Deeper insights into the ecology of these organisms in the McMurdo Dry Valleys also have implications for the search for complex unicellular life in our universe. I discuss the theoretical underpinnings of searching for these forms of life outside of Earth, conclude that they are likely to occur, and postulate how future missions could practically search for complex unicells.

No description available.

No description available.

No description available.

The distribution of massive ground ice in the ground in Taylor Valley of the McMurdo Dry Valleys, Antarctica, is quite unknown, and could provide answers to questions such as where the ice comes from, if it has been affected and removed by proglacial lakes and how landscapes underlain by massive ground ice responds to climate change. It could also be a source for atmospheric information in the past and hence a key in climate research. The main goal with this project was therefore to map the distribution of massive ground ice mainly in Taylor Valley, but also in the adjacent Salmon Valley and Wright Valley, using ground penetrating radar to see how the distribution varied and if there was any spatial patterns. The technical computing programme MATLAB was used for editing of the raw radar data, merging of GPR profiles and digitalization of reflectors for possible massive ground ice and several compilations of different files. The data obtained from MATLAB was imported and interpreted using the geographic information system ArcGIS. A series of histograms showing the distribution of massive ground ice depending on the parameters elevation, slope and aspect were made by using the spreadsheet application Microsoft Excel. The results showed that the distribution of massive ground ice was more common at elevations up to 200 m, at the mouth of the valleys and also more frequent in Taylor Valley than in Wright Valley. There was a slightly higher amount of massive ground ice at northeast-east aspects, probably due to different incoming solar radiation. The lack of, or not that prominent, differences for slope and aspect can be due to lack of data, a not enough detailed digital elevation model or that it have existed for a too short period of time to display big differences caused by effects from these parameters. The higher frequency of massive ground ice in Taylor Valley can be due to a thicker sediment cover when compared with the situation in Wright Valley. The distribution of massive ground ice at different slopes seems to follow the distribution of radar measurements, whereas the origin of the massive ground ice and sediment cover can be responsible for the distribution across different elevations. The reason why massive ground ice still occurs despite the existence of Glacial Lake Washburn that previously occupied Taylor Valley could be that the glacial lake did not remain for a sufficiently long time to melt all the massive ice. Massive ground ice is very common in a zone that is believed to be very susceptible for future warming, which means that changes that already have been observed in areas rich in massive ground ice can continue to happen and changes in other areas with massive ice can be enabled. The ice can thus play a major role in the development of the landscape in the McMurdo Dry Valleys depending on the amount of warming. / Markis kan hittas i mark som har temperaturer under 0°C under åtminstone 2 år i följd och därav klassas som permafrost, skillnaden mellan markis och permafrost är däremot att permafrost inte behöver vara just is utan kan enbart vara kall mark. För att markis ska klassas som massiv is så ska andelen is i marken vara minst 250 % jämfört med vikten på torr jord. Utbredningen av sådan massiv is i Taylor Valley i McMurdos torrdalar på Antarktis är inte helt känd, och kunskapen om att veta vart den finns (om den finns) skulle kunna ge svar på frågor som vart den kommer ifrån, om den har påverkats och smält bort av isuppdämda sjöar och hur landskap som är grundade av massiv markis påverkas av klimatförändringar. Isen skulle även kunna vara en informationskälla för tidigare atmosfäriska förhållanden. Huvudsyftet med detta arbete var därför att kartlägga utbredningen av massiv is främst i Taylor Valley, men även i de närliggande dalarna Salmon Valley och Wright Valley, och undersöka hur utbredningen varierar beroende på olika landskapsegenskaper som påverkar dess förekomst. Datorprogrammet och programspråket MATLAB användes för att editera rådatat från radar-mätningarna i området, samt för att sammanföra och digitalisera horisonter för möjlig massiv markis i radarfigurerna och för ett antal sammanställningar av olika filer. Data erhållet från MATLAB importerades till det geografiska informationssystemet ArcGIS där det kunde visualiseras i kartor och tolkas. Ett antal histogram skapades i kalkylprogrammet Microsoft Excel för att visa frekvensen av massiv markis vid olika höjder, sluttningsvinklar och olika väderstrecksriktningar. Resultaten visade att det var mer vanligt med massiv is höjder upp till 200 m, vid mynningarna av dalarna samt i Taylor Valley jämfört med Wright Valley. Det var en aning mer vanligt med massiv markis vid nordöst-östliga sluttningsriktningar, vilket antagligen beror på olika mängder inkommande solstrålning till de olika riktningarna. Avsaknaden av, eller inte så märkbara, skillnader för olika sluttningsvinklar och riktningar kan bero på att mängden data var för liten, att höjdkartan inte var tillräckligt detaljerad eller att isen inte har funnits tillräckligt länge för att bli påverkad av dessa parametrar. Anledningen till att det finns mer massiv markis i Taylor Valley än i Wright Valley kan vara att det skyddande sedimenttäcket är tunnare i Wright Valley än i Taylor Valley. Frekvensen av massiv markis vid olika sluttningsvinklar verkar bero på det totala antalet mätningar gjorda, fler mätningar leder till en högre frekvens av markis, medan dess ursprung samt det antagna tunnare sedimenttäcket på högre höjder kan vara anledningen till de olika frekvenserna av massiv markis vid olika höjder. Anledningen till varför det fortfarande finns massiv markis trots existensen av den isuppdämda sjön Washburn som tidigare fanns i Taylor Valley, och att isen således inte helt har smält bort på grund av sjön, kan vara att den fanns under en för kort tid så att de långsamma termodynamiska processerna som skulle orsaka smältningen inte hann agera tillräckligt länge för att smälta all is. Den massiva markisen är vanlig i en zon som tros vara väldigt mottaglig för framtida uppvärmning, vilket betyder att landskapsförändringar som redan har observerats i områden med mycket massiv markis kan fortsätta att ske samtidigt som andra områden med massiv markis kan börja förändras. Isen kan därför spela en stor roll i landskapsutvecklingen i McMurdos torrdalar beroende på hur mycket varmare det blir i området.

The natural environments of organisms present a multitude of biotic and abiotic challenges that require both short-term ecological and long-term evolutionary responses. Though most environmental response studies have focused on effects at the ecosystem, community and organismal levels, the ultimate controls of these responses are located in the genome of the organism. Soil nematodes are highly responsive to, and display a wide variety of responses to changing environmental conditions, making them ideal models for the study of organismal interactions with their environment. In an attempt to examine responses to environmental stress (desiccation and freezing), genomic level analyses of gene expression during anhydrobiosis of the Antarctic nematode Plectus murrayi was undertaken. An EST library representative of the desiccation induced transcripts was established and the transcripts differentially expressed during desiccation stress were identified. The expressed genome of P. murrayi showed that desiccation survival in nematodes involves differential expression of a suite of genes from diverse functional areas, and constitutive expression of a number of stress related genes. My study also revealed that exposure to slow desiccation and freezing plays an important role in the transcription of stress related genes, improves desiccation and freezing survival of nematodes. Deterioration of traits essential for biological control has been recognized in diverse biological control agents including insect pathogenic nematodes. I studied the genetic mechanisms behind such deterioration using expression profiling. My results showed that trait deterioration of insect pathogenic nematode induces substantial overall changes in the nematode transcriptome and exhibits a general pattern of metabolic shift causing massive changes in metabolic and other processes. Finally, through field observations and molecular laboratory experiments the validity of the growth rate hypothesis in natural populations of Antarctic nematodes was tested. My results indicated that elemental stoichiometry influences evolutionary adaptations in gene expression and genome evolution. My study, in addition to providing immediate insight into the mechanisms by which multicellular animals respond to their environment, is transformative in its potential to inform other fundamental ecological and evolutionary questions, such as the evolution of life-history patterns and the relationship between community structure and ecological function in ecosystems.