A unifying goal of plant systematics is in pursuit of a natural system that means phylpgenetic relationships. We can construct it by means of various characters. There are many arguments about generic circumscription of Pteridophyta. It¡¦s worth while to reexamine some characters, such as trichome. Trichomes have long been considered one of the most important characters by pteridologists. However, The study of trichomes in Taiwan is very rare. In the present study, trichome morphology is used to assess phylogenetic relationships among genera of Dryopteridaceae (sensu Kramer et al.) . The observation of trichomes focus on hairs on the lamina and scales on the base of stipes. Lamina surface hairs are classified into unicellular hairs, club-like unicellular glands, uniseriate hairs, spine-like hairs, appressed glandular hairs, uniseriate hairs with a glandular head and verruca. Stipe base scale margins are classified into entire, serrate, unicellular branch, uniseriate branch, multicellular branch with a glandular head, and dorsi-ventral branch. Color distribution and branch dimension of scales were also good differentiation characters. The main taxonomic conclusions are as follows: (1)Trichome characters support the distinctness of Nothoperanema, Peranema, Polystichm, Dryopsis, Ctenitis, Tectaria, Athyrium, Cystopteris, Acystopteris, and Woodsia; (2) Diplazium and Dryopteris are hererogenous and show little correaltion to exist system. The latter two genera need further research.