Dans ce travail, nous nous sommes intéressés à l’étude des dirofilarioses chez le réservoir canin « le chien » ainsi que chez les vecteurs « moustiques », en particulier, en ce qui concerne la détection, la surveillance et la prophylaxie. Le premier objectif était de développer une PCR duplex en temps réel ciblant le gène COI capable de détecter et de différencier simultanément D. immitis et D. repens. Ainsi, nous avons détecté par cet outil moléculaire, pour la première fois en France, D. immitis et D. repens chez des moustiques tigre "Aedes albopictus". Nous avons, de plus, confirmé la présence de l’infection à D. immitis chez les chiens du nord d'Algérie.Le deuxième objectif était d'évaluer l’intérêt de la spectrométrie de masse MALDI-TOF MS pour la détection de changements dans les profils protéiques d'Aedes aegypti infectés expérimentalement avec des nématodes filaires (D. immitis, Brugia malayi et B. pahangi). Les résultats obtenus montrent la capacité du MALDI-TOF MS à différencier des moustiques infectés et non infectés par les filaires. Ainsi, les meilleurs taux de classification correcte obtenus sont 94,1, 86,6, 71,4 et 68,7% pour les non infectés versus ceux infectés, respectivement, par D. immitis, B. malayi et B. pahangi.Le troisième objectif de ce travail était l’évaluation de l'efficacité anti-gorgement et insecticide d'un ectoparasiticide (Vectra® 3D) contenant trois principes actifs : le dinotéfurane, le pyriproxyfène et la perméthrine contre Ae. albopictus, l'une des principales espèces vectrices de Dirofilaria spp. Les résultats ont démontré que le DPP a une efficacité anti-gorgement et insecticide significative contre Ae. albopictus / In this work, we are interested in studying dirofilarial infections in dogs and vectors “Mosquitoes” especially detection, monitoring and prophylaxis. The first objective is to develop a real-time duplex PCR targeting the COI gene capable of simultaneously detecting and differentiating D. immitis and D. repens. Subsequently, we applied this tool to a canine dirofilariosis surveillance process in different endemic areas of Mediterranean basin (Corsica and Algeria). We have thus detected by this molecular tool for the first time in France, D. immitis and D. repens in Aedes albopictus mosquitoes. We reported, also, the presence of D. immitis in dogs from northern Algeria.The second aim was to assess whether the MALDI-TOF MS can detect changes in the protein profiles of Aedes aegypti infected experimentaly with filarial nematodes (D. immitis, Brugia malayi and B. pahangi). Obtained results showed the potential of MALDI-TOF MS as a reliable tool for differentiating non-infected and filariae-infected Ae. aegypti mosquitoes with a best correct classification rate obtained from the thorax-head part with 94.1 and 86.6, 71.4 and 68.7% for non-infected and D. immitis, B. malayi and B. pahangi infected mosquitoes respectively.The third aim of this work has focused on the evaluation of the anti-feeding and insecticidal efficacy of an ectoparasiticide (Vectra® 3D) containing three active ingredients: dinotefurane, pyriproxyfen and permethrin (DPP) against Ae. albopictus. Results demonstrated that the DPP combination has significant anti-feeding and insecticidal efficacy against Ae. albopictus for at least 4 weeks.

The aim of the thesis was to verify use of peracetic acid (PAA) in production conditions for ectoparasites control in carp pond farming. First test was about evaluation of organic load in pond water on speed PAA decay in two temperature regime (11 15 and 21 °C). Results showed that between 3 6 hours from PAA application the concentration of PAA was under a half of the initial concentration (6 mg.l-1). Also statically important differences (p= < 0.05) were found in speed of decay between samples with organic load (3 samples from ponds) and samples without organic load (distilled and tap water) in both temperature regimes. Application of PAA during the transport of fish into the transport boxes was performed in three age categories (1, 2 and 3 years old common carps), where the fish were exposed for 60 90 minutes in 1.5 mg.l-1 PAA as Persteril 36. Parasitic examinations of skin and gills showed reduction of number protozoal parasites (Trichodina spp. and Apiosoma spp.) in individuals comparing with control. Against other detected parasites was PAA ineffective. Statistical evaluation showed significant (p= <0.05) decrease in prevalence only in 3 cases out of 19 in total. Long-term four days long fish bath of carps which were heavily infested by Saprolegnia parasitica in 1.5 mg.l-1 PAA an positive effect on the overall survival was found. In the control group mortality reached 100 % the third day of trial, in contrast with treated group where was Saprolegnia parasitica succesfuly eliminated and overall moratily was 40 %. Continual fourteen days long application of PAA by peristaltic pump did not proved.