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It has been theorized that most proteins are under selection pressure to be soluble in crowded cellular spaces. To maintain solubility a proteins’ aggregation propensity should be inversely proportional to their maximum likely concentration. This theory was examined by comparing the proteome of the model organism S. cerevisiae, which has previously undergone a Whole Genome Duplication (WGD) event to the proteome of the closely related yeast K. waltii, which has not undergone WGD. This comparison revealed the following: 1) Predicted aggregation propensities are higher in S. cerevisiae than K. waltii. 2) Aggregation propensity does not predict which genes reverted to a single copy after WGD. 3) In genes which were retained as duplicates in S. cerevisiae after WGD, aggregation propensities rose from the inferred common ancestral protein. 4) Genes retained as duplicates showed less of an increase relative to their homologues in K. waltii than genes which were not retained as duplicates. 5) The relationship between the log predicted aggregation propensity and log mRNA expression level or log protein abundance was not linear as previously predicted. These results suggest that while there is broad selection pressure for reduced aggregation pressure for genes which have been duplicated, the precise relationship between aggregation propensity and gene expression is more complicated than previously predicted. These results also allow speculation that the whole genome duplication in S.cerevisiae may have been made possible by a general relaxation of aggregation-related selection pressure. / text

Canadians have developed a vocabulary of regionalism, a cultural shorthand that divides Canada into easily-described spaces: the Arctic, the Prairies, the Maritimes, and Central Canada, for example. But these artificial divisions obscure the history of edge places whose identity is drawn from more than one region. The region north of Prince Albert, Saskatchewan, is a place on the edge of the boreal forest whose self-representations, local history, and memorials draw heavily on a non-prairie identity. There, the past is associated with the forest in contrast to most Canadians' understanding of Saskatchewan as flat, treeless prairie. This dissertation presents the history of the north Prince Albert region within a framework that challenges common Saskatchewan and Canadian stereotypes. Through deep-time place history, layers of historical occupation in the study region can be compared and contrasted to show both change and continuity. Historical interpretations have consistently separated the history of Saskatchewans boreal north and prairie south, as if the two have no history of interchange and connection. Using edge theory, this dissertation argues that historical human occupation in the western interior found success in the combination of prairie and boreal lifeways.<p> First Nations groups from both boreal forest and open plain used the forest edge as a refuge, and to enhance resilience through access to resources from the other ecosystem. Newcomer use of the prairie landscape rebranded the boreal north as a place of natural resources to serve the burgeoning prairie market. The prairies could not be settled if there was not also a nearby and extensive source for what the prairies lacked: timber and fuel. Extensive timber harvesting led to deforestation and the rise of agriculture built on the rhetoric of mixed farming, not King Wheat. The mixed farming movement tied to landscape underscored the massive internal migrations from the open prairies to the parkland and forest edge.<p> Soldier settlement, long viewed as a failure, experienced success in the north Prince Albert region and gave a model for future extensive government-supported land settlement schemes. South-to-north migration during the 1920s was based on a combination of push and pull factors: drought in the Palliser Triangle; and a strengthening northern economy built on cordwood, commercial fishing, freighting, prospecting and fur harvesting, as well as mixed farming. The economy at the forest edge supported occupational pluralism, drawing subsistence from both farm and forest, reflecting the First Nations model. As tourism grew to prominence, the Saskatchewan dual identity of prairie/forest led to the re-creation of the north Prince Albert region as a new vacationland, the Playground of the Prairies. The northern forest edge drew thousands of migrants during the Great Depression. Historical analysis has consistently interpreted this movement as frantic, a reactionary idea without precedent. Through a deep-time analysis, the Depression migrations are viewed through a new lens. The forest edge was a historic place of both economic and cultural refuge and resilience predicated on the Saskatchewan contrast of north and south.

Canadians have developed a vocabulary of regionalism, a cultural shorthand that divides Canada into easily-described spaces: the Arctic, the Prairies, the Maritimes, and Central Canada, for example. But these artificial divisions obscure the history of edge places whose identity is drawn from more than one region. The region north of Prince Albert, Saskatchewan, is a place on the edge of the boreal forest whose self-representations, local history, and memorials draw heavily on a non-prairie identity. There, the past is associated with the forest in contrast to most Canadians' understanding of Saskatchewan as flat, treeless prairie. This dissertation presents the history of the north Prince Albert region within a framework that challenges common Saskatchewan and Canadian stereotypes. Through deep-time place history, layers of historical occupation in the study region can be compared and contrasted to show both change and continuity. Historical interpretations have consistently separated the history of Saskatchewans boreal north and prairie south, as if the two have no history of interchange and connection. Using edge theory, this dissertation argues that historical human occupation in the western interior found success in the combination of prairie and boreal lifeways.<p> First Nations groups from both boreal forest and open plain used the forest edge as a refuge, and to enhance resilience through access to resources from the other ecosystem. Newcomer use of the prairie landscape rebranded the boreal north as a place of natural resources to serve the burgeoning prairie market. The prairies could not be settled if there was not also a nearby and extensive source for what the prairies lacked: timber and fuel. Extensive timber harvesting led to deforestation and the rise of agriculture built on the rhetoric of mixed farming, not King Wheat. The mixed farming movement tied to landscape underscored the massive internal migrations from the open prairies to the parkland and forest edge.<p> Soldier settlement, long viewed as a failure, experienced success in the north Prince Albert region and gave a model for future extensive government-supported land settlement schemes. South-to-north migration during the 1920s was based on a combination of push and pull factors: drought in the Palliser Triangle; and a strengthening northern economy built on cordwood, commercial fishing, freighting, prospecting and fur harvesting, as well as mixed farming. The economy at the forest edge supported occupational pluralism, drawing subsistence from both farm and forest, reflecting the First Nations model. As tourism grew to prominence, the Saskatchewan dual identity of prairie/forest led to the re-creation of the north Prince Albert region as a new vacationland, the Playground of the Prairies. The northern forest edge drew thousands of migrants during the Great Depression. Historical analysis has consistently interpreted this movement as frantic, a reactionary idea without precedent. Through a deep-time analysis, the Depression migrations are viewed through a new lens. The forest edge was a historic place of both economic and cultural refuge and resilience predicated on the Saskatchewan contrast of north and south.