Specific objective: Guided tissue regeneration (GTR) aims to regenerate the lost attachment apparatus caused by periodontal disease through the use of a barrier membrane. For the GTR procedures to be successful, barrier membranes are required to be present at the surgical site for an extended period of time (weeks to months). Synthetic membranes have the advantage of prolonged presence in a wound site; however, they do not actively contribute to wound healing. Biologic membranes are recognized by the host tissue and participate in wound healing but have the disadvantage of early resorption. Therefore, the goal of this study is to create and characterize a hybrid barrier membrane that contains biologically active fibrin matrix within a synthetic polymeric electrospun scaffold. Method: Fibrin matrices and fibrin-incorporated electrospun scaffold were created from fresh frozen plasma at three different centrifugation conditions 400g for 12 minutes, 1450g for 15 minutes and 3000g for 60 minutes. Following centrifugation, half of the membranes were crosslinked with 1% genipin. Biological stability of these scaffolds was evaluated by resistance to trypsin while their mechanical properties were characterized by MTS Bionix Uniaxial Tensile Testing System. Continuous data was analyzed by ANOVA to detect differences between groups (p=0.05). Results: The addition of an electrospun scaffold to the fibrin matrix led to improvements in the mechanical properties as evidenced by an increase in the modulus (p<0.0001), strain at break (p<0.0001) and energy to break (p<0.0001). The effect of crosslinking was marginal but not statistically significant to the mechanical properties of fibrin matrices or the fibrin incorporated scaffold. However, crosslinking did significantly increase resistance against enzymatic degradation by trypsin (p<0.0001). Lastly, centrifugation speeds at 400g and 1450g showed similar mechanical properties and biologic stability; meanwhile 3000g negatively impacted the properties of the scaffold. Conclusion: Fibrin-incorporated electronspun scaffold exhibits enhanced mechanical and biologic stability compared to fibrin matrices alone. Moreover, crosslinking improves the biologic stability of the novel biomaterial. All these characteristics of the fibrin-incorporated matrix make this membrane a potentially more ideal barrier for GTR procedures to enhance periodontal wound healing.

yes / A major challenge in the production of 3D tissue engineered skin is the recreation of the basement membrane region to promote secure attachment and yet segregation of keratinocytes from the dermal substitute impregnated with fibroblasts. We have previously shown that simple electrospun scaffolds provide fibres on which the cells attach, proliferate, and self-sort into epithelium and dermis. In a development of this in this study tri-layered scaffolds were then electrospun from poly L-lactic acid and poly hydroxybutyrate-co-hydroxyvalerate. In these a central layer of the scaffolds comprising nano-porous/nano-fibrous poly hydroxybutyrate-co-hydroxyvalerate fibres was interwoven into the bulk micro-porous poly L-lactic acid microfibers to mimic the basement membrane. Keratinocytes and fibroblasts seeded onto these scaffolds and cultured for 2 weeks showed that neither cell type was able to cross the central nano-porous barrier (shown by SEM, and fluorescence monitoring with CellTracker™) while the micro-fibrous poly L-lactic acid provided a scaffold on which keratinocytes could create an epithelium and fibroblasts could create a dermal substitute depositing collagen. Although cells did not penetrate this barrier the interaction of cells was still evident-essential for epithelial development.

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Title: Methodological detection solution scaffolds response to mechanical stress, depending on the degree of hydration Objectives: Determining the extent of lateral deformation u scaffolds made of PVA polymer electrospinning technique. Identify the extent of differences in transverse deformation for different groups of nanofiber scaffolds made of PVA polymer electrospinning technique. Methods: Research scaffolds, we used a measuring device μ-tester, which has two jaws. For the measurement, we chose uniaxial tension test in -tester and record the fluorescence microscope was used with HD camera Olympus 320 for online video recording. Results: The results of this study showed that the ratio of the samples U: L and crosslinking time affects the degree of lateral deformation of the samples scaffolds. Samples scaffolds are compressible, some groups even reached the limits of incompressibility 0.5 Poisson's ratio. Keywords: Poisson, Poisson's ratio, scaffold, nanofiber scaffold, scaffold hydrated, electrospun scaffold, lateral deformation