Thesis (Ph. D.)--University of Wisconsin--Madison, 1984. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 99-115).

No description available.

Horseradish peroxidase (HRP) was applied to the rostral end of sectioned hepatic vagi (HV) . Subsequently, a count of HRP—labeled cells in the nodose ganglia (NDG) yielded an estimate of the minimum number of afferent fibers in the HV of 139. HHP labeled cells were found only in the left NDG and were diffusely spread throughout the ganglia. No HRP labeling was found in areas of the brain previously reported to contain vagal afferent projections. In three cases small numbers of HRP labeled cell bodies were seen in the dorsal motor nucleus (DMN) . The NDG were organized with cell bodies on the sides and their processes and fibers of passage in the center. The NDG have an apparent population of two cell types; large sensory neurons and smaller glial cells. However, the possibility of a population of smaller sensory cells is discussed. An average of total sensory cell counts for three NDG yielded an estimate of 9115 sensory cells.

Egzistuoja du širdies sensorinių galūnėlių tipai – sudėtingos neinkapsuliuotos ir tinklinės galūnėlės. Šio tyrimo tikslas buvo ištirti sensorinių galūnėlių morfologiją pelės širdies pagrinde ir jų vizualizavimo galimybę skirtingais antikūnais. Tyrimo rezultatai parodė, kad tiek sudėtingos neinkapsuliuotos, tiek tinklinės sensorinės nervinės galūnėlės išryškinamos imunohistochemiškai su antikūnu prieš proteino geno produktą PGP 9.5 (AbD Serotec, JAV). Sudėtingos neinkapsuliuotos galūnėlės paplitusios kaudalinės, kairiosios bei dešiniosios kranialinių venų žiočių srityje. Tinklinės galūnėlės tolygiai pasiskirsto visame širdies pagrinde visuose trijuose širdies sienos sluoksniuose (epikarde, miokarde ir endokarde). Sudėtingos neinkapsuliuotos galūnėlės kaudalinės venos žiočių srityje turi mažiausią plotą palyginus su sensorinėm galūnėlėm šalia kitų dviejų venų žiočių. / There are two types of cardiac sensory endings – complex unencapsulated endings and end-nets. The aim of this study was to examine morphology of sensory terminals in the basis of mouse heart and ability of different antibodies to visualize these terminals. Our results showed that both end-net and complex unencapsulated endings can be visualized with PGP 9.5 (AbD Serotec, USA). Complex unencapsulated endings are localized in heart wall epicardium near the apertures of caudal, right cranial and left cranial veins in the right atrium. End-net terminals are diffusely distributed in all three layers (endocardium, myocardium, epicardium) of heart wall. Complex unencapsulated endings near the caudal vein aperture have the lowest area compared with the sensory endings near the other two veins apertures.

The compartmental hypothesis of neurodegeneration proposes that the neurone, long recognized to consist of morphologically and functionally distinct compartments, also houses distinct degeneration mechanisms for the soma, axon and nerve endings. Support for this hypothesis is provided by the phenomenon of the WldS (for Wallerian Degeneration, slow) mouse, a mutant in which axons survive several weeks after transection, rather than degenerating within 24-48 hours as in wild type mice, by virtue of expression of a chimeric Nmnat1/Ube4b protein. In this thesis I used the WldS-mouse to re-examine and extend the theory of compartmental neurodegeneration by focusing specifically on sensory axons and endings; and finally by considering a fourth compartment, the dendrites. The first part of this thesis reports that Ia afferent axons and their annulospiral endings are robustly protected from degeneration in WldS mice. Homozygous or heterozygous WldS mice crossbred with transgenic mice expressing fluorescent protein in neurones were sacrificed at various times after sciatic nerve transection. Fluorescence microscopy of whole mount preparations of lumbrical muscles in these mice revealed excellent preservation of annulospiral endings on muscle spindles for at least 10 days after axotomy. No significant difference was detected in the protection with age or gene copy-number in contrast to the protection of motor nerve terminals, which degenerate rapidly in heterozygote and aged homozygote WldS mice. In an attempt to explain the difference in motor and sensory protection by WldS, examination of three hypotheses was undertaken: a) differences in protein expression, tested by western blot and immunohistochemistry; b) differences in the degree of neuronal branching, tested through examination of g-motor axons and endings which have a degree of branching intermediate to motor and sensory neurons; and c) differences in the activity in the disconnected stumps, through primary culture of the saphenous and phrenic nerve, selected because they comprise largely pure sensory and motor axons respectively. The data suggest that none of these hypotheses provides a sufficient explanation for the difference between sensory and motor protection by WldS. The last part of this thesis attempts to extend the theory of compartmental degeneration. I examine a system for investigation of WldS-mediated protection of dendrites. In preliminary experiments retinal explants from transgenic mice expressing YFP in a subset of retinal ganglion-cell neurones were cultured. The dendritic arbours of these cells were shown to be amenable for repeated visualization and accessible to injury and monitoring of degeneration. Overall the data in this thesis suggest that the level of WldS -mediated protection conferred to an axon or axonal endings varies between different neuronal types. This has implications for the potential applications of WldS research to clinical problems. Specifically, the data imply that sensory neuropathies may benefit more than motor neuropathies from treatments based on the protective effects of WldS. These findings in sensory neurones also challenge some of the assumptions made about WldS- mediated protection of neurones, for example the extent of the age-effect on axonal endings. Further investigation of WldS-mediated protection in the CNS could give renewed impetus to attempts to discover targets for treatment in common neurodegenerative diseases. Finally, a system for investigation of dendritic degeneration has been piloted, suggesting that molecules involved in the degeneration of dendrites or in protection from this degeneration may be amenable to investigation in this system, prospectively extending the compartmental hypothesis of neuronal degeneration.

The current study examines aspects of the Russian language that are particularly challenging for English-speaking students. It focuses on the complexity of Russian's grammatical morphology, specifically Russian case endings. In this study, methods and theories from the field of Second Language Acquisition (SLA) are reviewed to support a study aide designed by the author to help students with the acquisition of Russian case endings. The proposed study aide consists of 24 sentences composed of high-frequency Russian words. The 24 sentences contain all regular (approximately 75) Russian case endings. The purpose of the model sentences is to teach case forms using a concrete language referent that can be manipulated during spontaneous speech. The proposed method was tested at the Missionary Training Center (MTC) in Provo, Utah to verify its validity in a formal classroom setting. Two sets of missionaries learning Russian were tested on their acquisition of Russian case forms over a period of three weeks. The control group, consisting of 34 missionaries, was tested first. This group was given the Russian case paradigm chart traditionally used at the MTC as a study aide for learning Russian case endings. The test group, consisting of 22 missionaries, was tested second. This group was given the model sentences in place of the traditional paradigm chart as a study aide for learning Russian case endings. The test-group missionaries were asked to memorize the model sentences. Each group was given a pretest on their knowledge of case forms on their first formal day of instruction. Three weeks later, they took a posttest. Missionaries from the test group were also given a quiz to test their knowledge of the model sentences. Gain scores for the two groups were analyzed statistically using a Two-way ANOVA (analysis of variance), finding the treatment for the control group (the paradigm chart) to be 0.2323 not significant, and the treatment for the test group (the model sentences) to be .0001 highly significant. This study suggests that by using model sentences as a companion to traditional case paradigms, a greater amount of case endings can be learned and retained in a shorter period of time.

No description available.

The purpose of this thesis is to evaluate the differences between 9-ending on promotion prices used by Thai retailers and Swedish retailers. The quantitative research approach is the chosen method in order to evaluate the differences between 9-ending on promotion prices used by Thai retailers and Swedish retailers. The collection of data will be mainly concentrated on primary data through brochures from retailers in both countries. Evaluation of the price endings for advertised products in the brochures from Thai and Swedish retailers founded the distinctions between the use of 9-ending prices in many ways such as the way the retailers used left and right digits for price endings, the way they set the prices compared between the same product category and the same product sub-category.

Thesis (Ph. D.)--University of Washington, 1998. / Vita. Includes bibliographical references (leaves [104]-122).

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / O interesse de pesquisadores pelo estudo do sistema proprioceptivo vem crescendo nas Ãltimas dÃcadas. Isto à decorrente da importÃncia da integridade deste sistema no tratamento das enfermidades ortopÃdicas. Os mecanorreceptores e as terminaÃÃes nervosas livres sÃo as unidades microscÃpicas fundamentais da propriocepÃÃo e do sistema nervoso aferente. Eles transmitem ao sistema nervoso central informaÃÃes fisiolÃgicas em forma de potencial de aÃÃo, quando as estruturas articulares, cÃpsula, ligamentos e mÃsculos, sÃo submetidas ao estresse do movimento. Foram avaliadas a presenÃa e a densidade dos mecanorreceptores na cÃpsula, ligamento da cabeÃa femoral e labrum acetabular de 45 quadris masculinos. Destes, 30 foram obtidos de pacientes do sexo masculino com artrose secundÃria (grupo I) durante artroplastia e 15 de cadÃveres frescos com articulaÃÃo do quadril normal (grupo II). A idade mÃdia do grupo I foi de 56,5 e do grupo II foi de 35,6 anos. Os fragmentos obtidos foram mensurados em 2mm e corados com cloreto de ouro a 1%. ApÃs fixaÃÃo os fragmentos foram seccionados com criostato, em sÃrie de 6 micrÃmetros de espessura e submetidos à microscopia de luz. Foram identificados trÃs tipos de mecanorreceptores: Ruffini, Pacini e Golgi, alÃm das terminaÃÃes nervosas livres de acordo com a classificaÃÃo de Freeman e Wike (1967). Em cada grupo foi determinado o nÃmero e a densidade de mecanorreceptores e em seguida foram comparados os resultados. Concluiu-se que o predomÃnio das terminaÃÃes tipo Pacini no gurpo controle foi significante quando comparado com os corpÃsculos tipo Rufini (p < 0,01) e Golgi (p < 0,001).Enquanto que no grupo artrose a densidade do tipo Golgi foi menor que o tipo Pacini(p < 0,001) e terminaÃÃes nervosas livres( p < 0,01).Por outro lado, quando comparados a densidade total das terminaÃÃes nervosas nos dois grupos observou-se uma reduÃÃo significante nos quadris artrÃsicos (p = 0.008). Isto sugere fortemente que a integridade do sistema proprioceptivo parece sofrer modificaÃÃes em quadris artrÃsicos, como conseqÃÃncia da reduÃÃo do nÃmero de terminaÃÃes nervosas. Estudos eletrofisiolÃgicos futuros serÃo necessÃrios para definir o papel das terminaÃÃes nervosas e o padrÃo proprioceptivo do quadril normal do quadril artrÃsico. / Proprioceptive studies have reported growing interest in investigators in the last few decades. This is result of relevant integrity of this system in the treatment of orthopedic diseases. Mechanoreceptors and free nerve endings are the microscopy basic units from proprioception and peripheral nervous system. They transmit to the central nervous system physiological information with detection threshold when the joint are submitted to mechanical stress. The presence and density of mechanoreceptors were investigated in the capsule, teres ligament and acetabulum labrum from 45 hips joint. Of these 30 were obtained from male patients (Group I) with secondary arthrosis at open arthoplasty and 15 of fresh cadaver with normal hip joint (Group II). The mean age of group I was 56,6 and the group II was 36,5 years. The fragments obtained were measured up to 2mm and stained with gold chloride 1%. After fixation the fragments were sectioned with cryostat at serial sections of 6Âm thickness and examined using light microscopy. We identified 3 types of mechanoreceptors: Ruffini, Pacini and Golgi corpuscles, as well free nerve endings according of Freeman and Wike classified 1967. Each group was determined the number and density of mechanoreceptors and then it was compared. We conclude that the number of Pacini type was significative when it was compared with Rufini ( p < 0,01 ) and Golgi types (p< 0,001), in the normal group. However, the denstity of the Golgi type was minor compared to the Pacini ( p < 0,001) and free nerve ending ( p < 0,01 ) in the arthrosis group. Therefore, when the density total of nerve endings in normal hips were compared with arthosis hips we founded that the number decreased (p = 0.008). This is suggests strongly that the integrity of proprioceptive system seems to be modified by arthosis in consequence of nerve endings numbers. In the future, electrophysiological studies will be necessary to determine the rule of nerve endings and proprioceptive system in the normal and arthosis hip.