The human genome comprises 8% sequences of retroviral origin, so-called human endogenous retroviruses (HERVs). Most of these proviral sequences are defective, but some possess open reading frames. They can lead to the formation of viral transcripts, when activated by intrinsic and extrinsic factors. HERVs are thought to play a pathological role in inflammatory diseases and cancer. Since the consequences of activated proviral sequences in the human body are largely unexplored, selected envelope proteins of human endogenous retroviruses associated with inflammatory diseases, namely HERV-K18, HERV-K113, and HERV-Fc1, were investigated in the present study. A formation of glycosylated envelope proteins was demonstrated in different mammalian cell lines. Nevertheless, protein maturation seemed to be incomplete as no transport to the plasma membrane was observed. Instead, the proteins remained in the ER where they induced the expression of genes involved in unfolded protein response, such as HSPA5 and sXBP1. Furthermore, low expression levels of native envelope proteins were increased by codon optimization. Cell-free expression systems showed that both the transcriptional and translational level is affected. By generating different codon-optimized variants of HERV-K113 envelope, the influence of single rare t-RNA pools in certain cell lines was demonstrated. The mRNA secondary structure also appears to play an important role in the translation of the tested viral envelope proteins. In summary, the formation of certain HERV proteins is basically possible. However, their complete maturation and thus full biologic activity seems to depend on additional factors that might be disease-specific and await elucidation in the future.

In 1800, Volta, inspired by the electric organs of a genus of electric fish, the Electrophorus, invented the first electric batteries, which were termed "artificial electric organs." Since then, the far-reaching implications of the fishes’ electrogenesis have come under greater attention and interest. In these fishes, the electric organ resembles a series of batteries. The electric organs are formed by electrocytes (the "batteries") with a distinct cytomorphology for discharging and charging. Although the arrangements of electrocytes in the electric organ are well-understood, the mechanisms involved in generating electric discharges within equivalent circuits remain unclear. In this thesis, the first element consists of adapting spatially defined models that we use to investigate the process of electrocyte charging and recharging under the added assumption of ion homeostasis, the process by which a cell restores its internal milieu. The study focuses on Eigenmannia and Electrophorus, two genera of electric fish. Eigenmannia's steady high-frequency dipole oscillator-like electric organ discharges enables electro-sensing and electro-communication, whilst Electrophorus's brief taser-like electric bursts serve as tetanizing predatory assaults. In addition, the second section of this study proposes a one-dimensional charge difference model that focuses on the modification of endogenous electric fields resulting from the uneven distribution of ions in a homeostatic apparatus.

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[pt] O presente relatório objetiva apresentar o quanto as técnicas de negociações de conflitos e tomadas de decisões racionais estão ausentes na crise Venezuela Brasil. A não adoção de boas práticas negociais pelos agentes políticos e diplomáticos agrava o dia a dia dos povos envolvidos no litígio. A opção por modernos instrumentos negociais e a presença de um mediador endógeno é a proposta, pois facilitaria enormemente o desenvolvimento de uma governança regional. Para embasar a pesquisa debruça o relatório na literatura clássica e contemporânea, o que abrange temas como: conflict transformation, black negotiation, newgotiation, process alternative, Theory Problem Solving, o Nobel de economia de 2017 entre outras reflexões marcantes. / [en] This report aims to present how the techniques of conflict negotiation and rational decision making are absent in the Venezuela Brazil crisis. The nonadoption of good business practices by political and diplomatic agents aggravates the day-to-day of the peoples involved in the litigation. The option for modern negotiating instruments and the presence of an endogenous mediator is the proposal, as it would greatly facilitate the development of regional governance. To support the research, the report focuses on classical and contemporary literature, which covers topics such as: conflict transformation, black negotiation, newgotiation, process alternative, Theory Problem Solving, the Nobel economics of 2017 among other striking reflections.

This study explores how local community economic development actors in rural settings view prospects for bottom-up (i.e., endogenous) development through a case study of the Keremeos area in British Columbia. The study involved a basic descriptive analysis of the case study site drawing upon Statistics Canada data, historical records and local policy documents along with 11 semi-structured interviews of local development actors representing the varied geographic, jurisdictional and organizational interests comprising the Keremeos area development landscape. The findings indicate that not only do predominant institutional arrangements presuppose and eventuate rural inferiority and decline, they also tend to impose externalities for rural communities to contend with while failing to provide similar measures of in-kind support to mitigate such impacts. This stokes an erroneous sentiment that local government is responsible for precipitating endogenous development, limiting the extent to which community members work to actively operationalize it as a result. Such conditions ultimately lead rural communities to have no choice but to challenge predominant institutional arrangements in order to forge their own paths for realizing endogenous development. Indigenous communities offer hope for rural areas through increased guardianship programs and industry partnerships which help to challenge and rearticulate these arrangements to the advantage of their communities and interests. It is advisable that senior government work to provide rural communities with capacity to facilitate their own economic viability – reflecting local values, knowledge, identity and autonomy – thus working to counter the externalities and parameters which they and prevailing market forces have conceived for rural communities to persist within. / Graduate