This study is aimed at understanding the physical and chemical effects that changes in water content have on uranium leaching in sediment containing gravel. It was hypothesized that leaching will be more efficient under unsaturated conditions because flow will be restricted to the smallest pores and will have the most contact with the uranium contaminated sediment. Under saturated conditions, a large portion of the flow will bypass the < 2 mm material, and in turn not come into contact with uranium contaminated material. Batch adsorption and desorption experiments were performed on < 2 mm ERDF sediment to determine the linearity and reversibility of sorption processes and to aid in the interpretation of the leaching experiments. Results of the desorption experiments on aged, contaminated sediments show that the mass percent of sorbed U(VI) released to solution decreased as the sorbed concentration of U(VI) decreased. The opposite trend was observed on freshly contaminated sediments. This indicated that aging increased U(VI) affinity for the solid phase and was attributed to either the crystallization of calcite, which incorporated a portion of the sorbed U(VI) as it crystallized, or the presence of voids in basaltic lithic fragments accessed by diffusion. Column leaching experiments were performed at two water contents on artificially contaminated sediment collected from the Department of Energy’s (DOE) Hanford Site, Washington state. The sediment contained 81.3% gravel (> 2 mm) by mass. Non-reactive tracers were well fit with the convection-dispersion equation (CDE) at both high and low water contents indicating physical equilibrium. The column experimental data were fitted to an analytical solution to the CDE; the results of the modeling show an increase in the distribution coefficient (Kdeffective) with decreasing water content. Several potential explanations for this trend were proposed; one is based on a physical effect in which solute exposure to reactive surfaces changes as a function of water content and the others are based on results of the batch desorption experiments. This work has important implications for the Hanford Site where there is ongoing research regarding the persistence of U(VI) in the vadose zone and underlying aquifer.

Beaver Creek, located in North Knox County, Tennessee, is on the Tennessee 303(d) list as an impaired stream that fails to meet its designated uses. Phosphorus (P) is one of the major pollutants of the stream. High P levels within surface water can lead to water quality problems including low dissolved oxygen, overgrowth of algae, and eutrophication. Two sources, pasture grazing areas and major municipal point sources, have been identified as important contributors of P to Beaver Creek. The objective of this study was to analyze the total P and Mehlich III extractable P concentrations of sediments in Beaver Creek and determine if a P signal could be identified in sediments collected along two stream reaches, less than 1500 m long, adjacent to a cattle farm and a wastewater treatment plant (WWTP). Eight sites were sampled within Beaver Creek, divided between two locations. At each sample location, four sample sites were selected. One site, the “control” was upstream of the P input. One, the “source,” was immediately downstream of the P input. Two additional sites, one within 200 m and the second within 400 m downstream of the source, were selected at each location. Nine sediment samples were collected at each sample site. Sediments were analyzed for total P (SW-3050B) and Mehlich III extractable P. ANOVA was run between the sites at each location and t-tests were completed to look for significant differences and a downstream signal. At the cattle farm, P concentrations of sediments at the downstream 2 site were significantly higher than sediment P concentrations at the three other sites. However, unexpectedly high results from the control sample site, combined with unexpectedly low results from the source sample site made it difficult to assess whether the cattle farm was affecting sediment P in Beaver Creek. At the WWTP, the sediment P near the outfall was significantly higher than sediment P at the control. Sediments at the downstream 1 site had significantly higher P concentrations than sediments at the other three sites, indicating that the WWTP may be affecting sediment P in Beaver Creek. This study supports the hypothesis that increased P concentrations could be attributable to P inputs from a WWTP. However, further study is needed about the effects that pastures have on sediment P concentrations within Beaver Creek.

A gill filament-based assay for the cytochrome P450 1A (CYP1A)-catalysed activity ethoxyresorufin O-deethylase (EROD) was developed in rainbow trout (Oncorhynchus mykiss) and applied to Atlantic salmon (Salmo salar), Arctic charr (Salvelinus alpinus), Atlantic cod (Gadus morhua), saithe (Pollachius virens), and spotted wolffish (Anarhichas minor). Exposure to waterborne β-naphthoflavone (βNF; 10-6 M) induced branchial EROD activity in all species but the spotted wolffish. In rainbow trout exposed to low concentrations of benzo[a]pyrene (BaP; 10-9 M) and the textile dye indigo (10-8 M) the gills responded more rapidly than the liver to BaP, and indigo induced branchial but not hepatic EROD activity. A CYP1A-dependent BaP adduct formation was shown in gills of fish exposed to waterborne 3H-BaP, i.e. the adduct formation was enhanced by βNF and blocked by ellipticine (CYP1A inhibitor). The predominant location for BaP adducts was the secondary lamellae (most exposed part of the gill filament), whereas the CYP1A enzyme was also present in the primary lamellae of the gill filament. Hence, in addition to the cell-specific expression of CYP1A an important determinant for the localisation of adducts seemed to be the bioavailability of BaP. This idea is supported by the fact that the CYP1A enzyme was induced only in secondary lamellae by BaP (10-7 M) and indigo (10-6 M), whereas it was induced in both primary and secondary lamellae by 3,3´,4,4´,5-pentachlorobiphenyl (10-8 M). Apparently, readily metabolised inducers (BaP and indigo) are biotransformed in the secondary lamellae. My results show that gill filament EROD activity is a sensitive biomarker of exposure to waterborne dioxin-like pollutants, and that the assay has potential for use in monitoring. Furthermore, the results suggest that readily metabolised dioxin-like compounds absorbed via the gills may undergo first-pass metabolism in the gill cells and therefore remain undetected by monitoring of EROD activity in the liver.

Induction of the cytochrome P450(CYP)1A protein and the connected increase in 7-ethoxyresorufin O-deethylase (EROD) activity are common biomarkers in fish. Enhanced activity of this protein signals exposure to Ah-receptor agonists such as chlorinated dioxins, co-planar polychlorinated biphenyls (PCBs) and certain polycyclic aromatic hydrocarbons (PAHs). The EROD biomarker is commonly analyzed in liver microsomes. However, the gill is directly exposed to waterborne pollutants, and in this thesis the gill filament EROD assay was therefore evaluated as a monitoring tool for waterborne CYP1A inducers in fish. Originally developed in rainbow trout (Oncorhynchus mykiss), the assay was here applied in various limnic and marine species. Following exposure to low waterborne concentrations of the readily metabolized CYP1A inducers benzo(a)pyrene (BaP) and indigo, a strong EROD induction was observed in the gill but not in the liver. This likely reflected metabolic clearance of the inducers in gill and other extrahepatic tissues. The high sensitivity of the gill was confirmed in studies of fish caged in waters in urban and rural areas in Sweden where the gill consistently showed a more pronounced EROD induction compared with the liver and the kidney. Fish caged in the reference waters showed surprisingly strong gill EROD induction and CYP1A immunostaining. Consequently, there may be CYP1A inducers present in the aquatic environment that are not yet identified. The assay was further applied in Atlantic cod (Gadus morhua) as a biomarker of exposure to crude oil and produced water (PW) from oil fields in the North Sea. The assay was finally adapted to detect inhibiting compounds, and an imidazole, a triazole and a plant flavonoid turned out to be potent gill EROD inhibitors. The overall conclusion from the studies of this thesis is that the gill filament EROD assay is a practical and sensitive biomarker of exposure to waterborne CYP1A inducers in various fish species. The induction of gill EROD activity in fish also at the reference sites in the field studies calls for further studies on background contamination in Swedish waters.

Fungi are present everywhere in indoor and outdoor environments. Many fungi are toxigenic or pathogenic that may cause various public health concerns. Rapid detection, quantification and characterization of fungi in living and working environments are essential for exposure risk assessment to safe guard public health. Rapid and accurate detection and identification of fungi using molecular method require specific markers. In this thesis, partial mt SSU and LSU rDNA were amplified and sequenced from 31 fungal species of 16 genera. Sequence alignments showed that fungal mt SSU and LSU rDNA contained sufficient amount of variation for the development of markers that can discriminate even among closely related species. Forty-eight probes were designed and were verified as highly specific to 25 fungal species commonly detected in living and working environments. These specific probes would have potential applications in clinical diagnosis and public health-related environmental monitoring. Nested PCR is a highly sensitive and specific method. Based on the nuclear 18S rDNA sequence variation pattern, three nested PCR systems were developed to detect the conifer tree pathogen Gremmeniella abietina, an ascomycete fungus that causes stem canker and shoot dieback in many conifer species. The three nested PCR systems showed high specificity and sensitivity. These methods could have broad applications in forest protection and disease management programs. Quantitative real-time PCR offers the ability of simultaneous detection and quantification of DNA of a specific microbe in one reaction. Based on the 18S rDNA sequence, two real-time PCR assays were developed to detect and quantify Wallemia sebi, a deuteromycete fungus commonly found in agricultural environments and is suspected to be a causative agent of farmer’s lung disease. Both PCR systems proved to be highly specific and sensitive for W. sebi detection even in a high background of other fungal DNAs. Application of the real-time PCR methods in the quantification of W. sebi in the aerosols of a farm revealed a high concentration of W. sebi spores (107/m3). The study indicates that W. sebi is a dominant fungus in agriculture environments. Cladosporium spores are important aeroallergens, and prolonged exposure to elevated spore concentrations can provoke chronic allergy and asthma. A TaqMan probe and a SYBR Green I based real-time PCR assay were developed to detect and quantify Cladosporium in aerosols. The two real-time PCR systems proved to be highly specific and sensitive for Cladosporium. These methods were employed to quantify Cladosporium in aerosols of five different indoor environments. High spore concentration of Cladosporium (107/m3) was observed in a cow barn. Cladosporium spore concentration in paper and pulp factory and countryside house also exceeded threshold value for clinical significance. Prolonged exposure in these environments could impose certain health risk. Thus, monitoring Cladosporium spore concentration in indoor environments is important for indoor air quality control.

The U.S. Army Corps of Engineers, Nashville District, maintains ten reservoirs in the Cumberland River Basin in Kentucky and Tennessee, and has been monitoring sediment chemistry in the reservoirs since 1994. The purpose of this study is to evaluate the sediment data collected from the reservoirs from 1994 to 2010 to determine if there are any spatial patterns of the trace elements: arsenic, beryllium, cadmium, chromium, copper, lead, mercury, nickel, and zinc. The results indicated that trace element levels were consistent with national baseline concentrations measured by the U.S. Geological Survey. Center Hill reservoir had the greatest number of trace element concentrations (all except cadmium) that were significantly higher when compared to all other reservoirs. The degree of urbanization in the reservoir basins was based on population density from the 2000 Census and the percentage of developed land using the 2006 national land cover dataset. Aquatic toxicity values were used as a measure of sediment quality. The reservoirs with the worst aquatic toxicity rankings were not the most urban, instead they were the reservoirs with the longest retention times. Therefore, it may be concluded that retention time has a larger effect on Cumberland River Basin sediment concentrations than the type of land use or the degree of urbanization. The results also indicate that it may be prudent to include an evaluation of quality based on aquatic toxicity when monitoring sediment quality, and that when reservoirs are the subject of sediment quality assessments, the consideration of the physical properties of the reservoir, especially the retention time, is essential for a comprehensive evaluation. This may also imply that sediment quality in reservoirs may effectively be regulated by water resource management techniques at the reservoirs that affect retention time.

Environmental decision making processes are subject to diverse and at times conflicting pressures. On one hand, an enlightenment perspective places high value on scientific information about complex environmental phenomena, thus encouraging highly trained experts to perform research, interpret results, and provide advice to decision makers. On the other, international and domestic efforts to apply the concept of sustainable development tend to promote an enhanced role for non-expert knowledge and increased opportunities for public participation in decisions that affect local environments and livelihoods. Complicating this scenario further are debates within governments about how to allocate limited resources for environmental research and management. Citizen environmental monitoring initiatives provide an opportunity to examine these considerations as they play out in a variety of settings. From local, grassroots citizen groups to regional networks with government support, a wide range of monitoring programs exist that involve volunteers in gathering environmental information using scientific methods. Many groups attempt to apply their findings to planning initiatives, policy development or environmental law enforcement at local or regional levels. These efforts may blur the distinction between scientific and local knowledge, while raising questions about the relative legitimacy of experts and citizens as producers of knowledge for use in environmental decision making. Using a case study approach, this thesis explores factors affecting the application of information gathered through citizen environmental monitoring programs to decision making processes and outcomes in Comox Valley, British Columbia, and Hamilton and Muskoka, Ontario. Semi-formal interviews were conducted in all three locations with coordinators of citizen groups that perform environmental monitoring, as well as with government representatives who have some involvement with the same citizen monitoring initiatives. Key themes affecting the use of citizen monitoring information emerged from the study, including political will on the part of local decision makers, scientific rigour and data quality of citizen monitoring efforts, and perceived legitimacy of citizen groups in terms of their organizational stability and reputations. Suggestions are presented for overcoming obstacles in each of these areas. The research also identifies further issues that affect the application of citizen-collected data such as the level of matching between the information priorities of citizen groups and governments, as well as collaborative arrangements between program partners. On these issues, interviewees shared their ideal scenarios for citizen monitoring programs with respect to funding, partnership strategies, and best roles for volunteers, citizen groups, and governments who are involved in citizen environmental monitoring programs. Based on the findings of this study, it is recommended that future research investigate further the issues of power sharing, agenda setting, and mutual trust between citizen groups and governments at the local level.

Reagent injections designed to provide in stiu mass destruction of soil and groundwater contaminants are commonly prone to failure due to inadequate distribution of the injected reagent. Reagent injections, in particular in situ chemical oxidation (ISCO) injections, require contact between the treatment reagent and targeted contaminant to allow for mass destruction in source zones and plumes. Subsurface heterogeneities that exist at all spatial scales prevent remediation specialists from accurate prediction of reagent distribution in the subsurface, even when significant site characterization and hydraulic testing has previously taken place. A prototype probe system was developed to provide real-time monitoring of the distribution of injected reagents. This thesis focused on laboratory testing of the system to verify that the design was capable of indicating the presence of an injected reagent in the field. Temperature and two-wire dipole resistance sensors were developed with low-cost materials to provide feedback on the electrical conductivity (EC) signatures produced by typical reagents mixed as salt solutions. Sensors were attached to sections of PVC conduit and wired to a data acquisition system to control measurements and store data. The temperature sensor was found to accurately respond to temperature changes in comparison to a commercial datalogger. Measured temperature differences between the constructed sensor and commercial datalogger were relatively constant, indicating that the constructed sensor could be calibrated to measurements from a commercial logger. Static cell experiments were conducted in beakers with varying concentration sodium chloride (NaCl), potassium permanganate (KMnO4) and sodium persulfate (Na2S2O8) solutions to determine dipole resistance sensor response to variations in EC. Different fixed resistors were wired with the dipole sensor circuit to determine the impact on sensor readings. Results indicated a nonlinear correlation between two-wire dipole resistance sensor response and increasing EC. Each constructed dipole sensor behaved uniquely. Raw sensor response was calibrated to EC by accounting for the influence of the fixed resistor. Data was fit to a second-order polynomial with form y = aEC2 + bEC + c, with r2 ranging from 0.92-1.00 for experiments with 4-6 measurement points. Calibrations were accurate within the range of EC for each static cell experiment; trends extrapolated beyond the measurement range were subject to significant error. The choice of fixed resistor did not appear to alter the accuracy of probe calibrations. Flow cell experiments were designed to analyze dipole resistance sensor response to continuous changes in EC. EC breakthrough curves (BTCs) were produced by injecting NaCl tracer solutions into the flow cell. Initial flow cell experiments conducted in an open water system showed agreement between dipole sensor measurement and handheld EC measurements on the rising limb of BTCs and divergence between the two datasets on the falling limb of BTCs. To resolve these issues, a more sophisticated tank with a porous medium was built and tested to compare sensor response from s prototype probe and a commercial EC datalogger. EC BTCs were measured under two scenarios: (1) conditions with deionized water (DI) circulating through the tank as the background solution, and (2) conditions with a simulated groundwater solution with elevated EC circulating through the tank as the background solution. BTCs produced agreement between EC recorded by the commercial logger and dipole resistance measurements for both the rising and falling limbs of BTCs. Results indicated the dipole resistance sensor was not capable of resolving fine changes in EC that occurred during breakthrough. A calibration of all in situ measurements from the experiments with porous medium confirmed the simulated groundwater experiments were subject to significantly less variability than the deionized water experiments. The calibration applied to the simulated groundwater measurements produced BTCs that matched very closely with those recorded by the commercial logger. Two field trials were also conducted during ISCO injections at contaminated sites where probes were installed in existing monitoring wells. The field trials did not successfully correlate dipole resistance sensor measurements with oxidant concentration. Observations from the second trial indicated the dipole sensor measurements correlated with EC of water samples. This work has provided a theoretical representation of two-wire dipole resistance sensor response to EC and has verified expected results through laboratory experiments. It has analyzed the influence of temperature and choice of fixed resistors on two-wire dipole resistance sensor readings, has extensively tested sensor response to EC during static cell and BTC experiments, and has displayed the prototype probe is capable of indicating the presence of injected reagents that have an EC signature. Further research avenues include pilot-scale testing in the field and developing a design for use with a direct-push rig.

Environmental decision making processes are subject to diverse and at times conflicting pressures. On one hand, an enlightenment perspective places high value on scientific information about complex environmental phenomena, thus encouraging highly trained experts to perform research, interpret results, and provide advice to decision makers. On the other, international and domestic efforts to apply the concept of sustainable development tend to promote an enhanced role for non-expert knowledge and increased opportunities for public participation in decisions that affect local environments and livelihoods. Complicating this scenario further are debates within governments about how to allocate limited resources for environmental research and management. Citizen environmental monitoring initiatives provide an opportunity to examine these considerations as they play out in a variety of settings. From local, grassroots citizen groups to regional networks with government support, a wide range of monitoring programs exist that involve volunteers in gathering environmental information using scientific methods. Many groups attempt to apply their findings to planning initiatives, policy development or environmental law enforcement at local or regional levels. These efforts may blur the distinction between scientific and local knowledge, while raising questions about the relative legitimacy of experts and citizens as producers of knowledge for use in environmental decision making. Using a case study approach, this thesis explores factors affecting the application of information gathered through citizen environmental monitoring programs to decision making processes and outcomes in Comox Valley, British Columbia, and Hamilton and Muskoka, Ontario. Semi-formal interviews were conducted in all three locations with coordinators of citizen groups that perform environmental monitoring, as well as with government representatives who have some involvement with the same citizen monitoring initiatives. Key themes affecting the use of citizen monitoring information emerged from the study, including political will on the part of local decision makers, scientific rigour and data quality of citizen monitoring efforts, and perceived legitimacy of citizen groups in terms of their organizational stability and reputations. Suggestions are presented for overcoming obstacles in each of these areas. The research also identifies further issues that affect the application of citizen-collected data such as the level of matching between the information priorities of citizen groups and governments, as well as collaborative arrangements between program partners. On these issues, interviewees shared their ideal scenarios for citizen monitoring programs with respect to funding, partnership strategies, and best roles for volunteers, citizen groups, and governments who are involved in citizen environmental monitoring programs. Based on the findings of this study, it is recommended that future research investigate further the issues of power sharing, agenda setting, and mutual trust between citizen groups and governments at the local level.

No description available.