Meerkats are known to strongly rely on chemical communication in social contexts. However, little is known about their use of the sense of smell in food detection and selection. The aim of the present study was therefore to assess whether meerkats are able to (1) detect hidden food using olfactory cues, (2) distinguish the odour of real food from a single food odour component, and (3) build an association between the odour of real food and a novel odour. I employed the buried food test, widely used with rodents to assess basic olfactory abilities, designed to take advantage of the propensity of meerkats to dig. I found that the meerkats were clearly able to find all four food types tested (mouse, chicken, mealworm, banana) using olfactory cues alone and that they successfully discriminated between the odour of real food and a food odour component. In both tasks, the animals dug in the food-bearing corner of the test arena as the first one significantly more often than in the other three corners, suggesting development of an efficient foraging strategy. No significant association-building between a food odour and a novel odour was found within the 60 trials performed per animal. I conclude that meerkats are able to use olfactory cues when foraging and that their sense of smell is well-adapted for recognizing specific odours of behavioural relevance. To the best of my knowledge, this is the first study to successfully employ the buried food test with a carnivore species.

Comparative studies of taste perception have found that primates may differ markedly in their sensitivity for substances perceived as sweet by humans. These findings raise questions about the reason that may underlie these differences in sweet-taste sensitivity between species. The aim of the present study was to assess the taste responsiveness of chimpanzees (Pan troglodytes verus) to seven substances tasting sweet to humans and to compare the results with those of other primate species. Using a two-bottle preference test (1 min) I found that the taste preference thresholds of the chimpanzees for five food-associated carbohydrates ranged between 20-30 mM for sucrose, 20-50 mM for fructose, 60-80 mM for glucose, 50-80 mM for maltose, and 30-80 mM for lactose. Taste preference thresholds for two steviol glycosides ranged from 0.04-0.05 mM for stevioside, and 0.03-0.05 mM for rebaudioside A. The chimpanzees displayed clear preferences for all sweet-tasting substances presented. In line with data obtained in other primates, the taste preference threshold of the chimpanzees for sucrose was lower compared to the other carbohydrates presented and the taste preference thresholds for stevioside and rebaudioside A were lower compared to sucrose. In general, the taste sensitivity of the chimpanzees fell into the range of data reported in other nonhuman primate species. Interestingly, the taste preference thresholds of the chimpanzees reported here are similar to the taste detection thresholds obtained in humans, despite the fact that the former are only a conservative approximation of an animal’s taste sensitivity. This suggests that chimpanzees may be as sweet-taste sensitive as humans.

Animal personality can be defined as a set of physiological and behavioral characteristics that differ between individuals, but are consistent over time and across situations. The evolution of individual differences in behavior that are consistent over time and situations is still not clear. Our understanding of why animals have personality can be improved by investigating the underlying physiological mechanisms of animal behavior. Serotonin is a key monoamine that serves as a physiological modulator of animal behavior. Selective serotonin reuptake inhibitors are a group of chemicals that increase levels of serotonin in the brain. Fluoxetine is one such chemical and is used to treat depression in humans. In the field cricket (Gryllus bimaculatus), increased levels of serotonin have been linked to higher activity and boldness, which are both personality traits. In the current study, the effects of induced serotonin on activity, exploration, boldness and aggression was investigated. My results show that injecting fluoxetine causes substantial changes in behavioral traits used to describe personality in field crickets. This result is opposite to previous studies, as serotonin induced individuals were less active, less explorative, and won less fights, compared to control individuals. This could be due to serotonin existing naturally within the circulatory system of the field cricket, whereas fluoxetine is a manufactured chemical intended for human receptors, or that fluoxetine has a similar effect in modulating personality in field crickets as in humans. Since fluoxetine acts similarly in field crickets as in humans, an increased understanding of the effects of induced serotonin on different behaviors in field crickets could be beneficial for treating psychological illnesses.

Interindividual differences in animal behaviour that are relatively consistent over time and context are referred to as animal personality. Personality has been recognized throughout the entire animal kingdom, in an array of species like molluscs, arthropods, fish, birds and mammals. The personality of non-human animals has been suggested to vary along five different axes, or continua; boldness-shyness, avoidanceexploration, activity, sociability and aggressiveness. Having a relatively fixed personality may seem nonadaptive compared to infinite behavioural plasticity so the individual would be able to respond adaptively to any changes in the environment. There can be physiological limitations to the phenotypic expressions of any trait, including behaviour. Variation in neuroendocrinology may thus explain why animals have personality. A candidate neurochemical that potentially proximately influences and forms personality, is serotonin (5- HT), one of the most omnipotent neurotransmitter of the animal body. In the many realms of the serotonergic system, there may arise individual differences which forms a proximate basis for differences in personality. In this review paper, I discuss the impact of the serotonergic system on a few different personality traits. Depending on the individual’s motivational state, serotonin can dampen or enhance aggression. Serotonin correlates negatively to anxious traits. Feeding behaviour is affected by serotonin in seemingly opposing directions. Overall, serotonin seem to underlie many behaviours that describe animal personality.

Animals in distress can experience an attenuation of their stress response if provided companionship. This thesis studied the social support phenomenon in 72 nine-week old juvenile pigs (Sus scrofa domesticus) of the crossbreed Yorkshire/Hampshire. Pigs were placed either alone or with a familiar or an unfamiliar age-matched conspecific of the opposite sex in a stunning box. Half of the groups were exposed to an air-filled foam inside of the box and the other half was exposed to an empty box. All pigs underwent an individual behaviour assessment at eight-weeks of age for a personality evaluation. The results of this thesis indicate that pigs were startled by the foam exposure as suggested by changes in activity, escape attempts and vocalisations. Indications of social buffering were found with regards to performance of escape attempts, altered activity patterns and engagement in affiliative social behaviours. Differences were observed in the social behaviour of familiar and unfamiliar pig pairs, suggesting that familiarity between pigs should be preferred from a welfare perspective. Correlations between possible personality traits and the pigs’ behaviour in the foam box study can be understood within the coping style framework. The results suggest that pigs show variation in their need for social support and that personality could have been a bias in interpreting the pigs’ behavioural response to the foam stunning situation. This thesis provide support that the welfare of pigs during foam stunning may be improved if pigs are stunned in groups of familiar individuals.

Stress cannot be fully avoided in an animal´s life, and the way that animals handle stress can be crucial for their health. Coping styles in animals are describing consistent sets of behaviour and physiological stress responses. Two forms of coping are typically described; a more proactive type that is more bold, active, and aggressive, and, in contrast, a less active, more shy reactive type. Coping styles can be used to learn how animals react to stress, and thus improve our handling of stress in captive animals. To investigate the existence of the reactive-proactive coping styles in the red junglefowl (Gallus gallus), both male and female red junglefowl were exposed to behavioural assays measuring boldness, activity, exploration, and aggression. These behavioural measures were compared to blood plasma concentrations of the stress hormone corticosterone, hence exploring the link between behaviour and physiological response to stress. I show positive correlations between boldness, activity, and exploration measured in a novel arena test, and boldness and activity measured in a novel object test. This somewhat confirms the inter-related behavioural aspect of reactive-proactive coping styles. Further, some of these behaviours correlated with post-stress concentrations of corticosterone, which somewhat links behaviour to the physiological aspect of coping styles. This study tentatively supports the reactive-proactive coping styles by showing a link between behaviours and the physiological stress response in the red junglefowl, with some similarities to what have been observed in other species across taxa.

Dogs have lived with us for thousands of years, so we have learned to understand one another. Dogs have been shown to synchronize their behavior with humans. Behavioral synchronization is characterizes by doing the same thing at the same time and in the same place as someone else. This synchronization is often dependent on the relationship and the emotional state of the individuals. The main aim in this study was to develop a method to test behavioral synchronization and also to investigate how this, but also other behaviors, are effect by preceding human-dog interactions. Privately owned dogs (N=21) were tested with three different treatments (play, pet or ignore). The test procedure consisted of the owner walking or standing still in a predefined pattern, while their dog was freely roaming. This study showed that dogs adapted their movement pattern to their owners. This was similar to previous studies showing that the method developed can be used to test behavioral synchronization between owner and dog. Dogs explored the test arena less after play interaction and they also tended to seek more eye contact with their owners, but the interactions did not affect how much time spent in human proximity, how much they followed them or on dogs movement pattern.

The evolutionary origins of human handedness are still unknown. The study of lateralized behaviour in our closest relatives, the nonhuman primates, is useful to clarify how this trait appeared and evolved in our species. In the present study, lateralized behaviour was assessed in a population of 32 free-ranging Mexican mantled howler monkeys (Alouatta palliata mexicana) for thirteen spontaneous motor patterns, at individual and group levels, as well as the effect that age, sex and posture have on its strength and direction. The studied population of howler monkeys displayed only few significant lateral biases at the individual level with single motor patterns (Binomial tests, p≤0.05). No biases towards the use of a particular limb or side of the body were found at a population level. Therefore, even though some individuals showed significant limb/side preference with single motor patterns, no signs of task specialization, side specialization, or true handedness were found. Similarly, no effects of sex, age or posture were found on the direction or strength of lateralized behaviour. The general absence of limb/side preferences found in this population may be due to the constraints imposed by the arboreal life and/or the type of diet. Possible causal agents of the few significant individual biases found here may be the presence of handicaps and/or experience. Further research is needed in order to assess whether the lack of human-like handedness reported in this study is only specific to the studied population, a general phenomenon of the genus Alouatta or perhaps of all the Platyrrhini.

Cognitive processes include biases, such as cognitive judgment bias. Cognitive judgment bias influences how the surrounding is interpreted, and this can differ between individuals. However, thus far no formal framework exists to understand how cognitive judgment bias works. Here I investigated how environmental factors and personality influence cognitive judgment bias in Gallus gallus chicks. First I investigated how two environmental factors affected the cognitive judgment bias of laying hen chicks. Chicks were exposed to stress and/or environmental enrichment, and tested in a cognitive judgment bias test before and after collective unpredictable stressors were presented. The results showed that chicks living in enriched environments were faster to reach all cues after the collective stressors than chicks living in non-enriched environments. Individual differences are often observed in animals, even when raised under identical conditions; therefore I also investigated if variation in personality influence cognitive judgment bias. Red junglefowl chicks were thereby raised in equal environments and exposed to personality assays in addition to a cognitive judgment bias test. I demonstrated that less nervous chicks were more optimistic towards ambiguous and negative cues than more nervous chicks. Also previous studies have found indications of connections between cognitive bias and environment or personality. I conclude that environmental enrichment can buffer the influence of stress on cognitive judgment bias and that personality has a small influence on interpretations of stimuli. In the future, experiments in this field should focus on exploring more aspects on how cognitive biases occurs to improve our understanding of cognitive processes.

Cognitive bias can be used when determining emotional states in animals by assessing the animal’s perception of an ambiguous stimulus. In the concept of animal welfare, both physical health as well as mental health of animals are involved. Therefore, cognitive bias can be a valuable tool in order to measure the mental health of an animal. The aim of this paper is to summarize and discuss how cognitive bias tests have been used to assess emotional states in dogs. Cognitive bias tests in dogs have been used to evaluate the emotional state of a dog with behavioural problems. It has also been shown to be useful when studying the effect of enrichment in the form of natural behaviour, such as being allowed to use its olfactory sense to a greater extent. Additionally, the connection between personality and cognitive bias is discussed. Hence, assessing the emotional state of dogs can be valuable in a welfare perspective.