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Characterization on the biochemical composition of collagen-hMSCs microspheres and their mechanical property during chondrogenic differentiationLi, Chun-hei. January 2009 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2010. / Includes bibliographical references (leaves 87-95). Also available in print.
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Structural changes of fibronectin during cell interactions and adsorption to surfaces measured using fluorescence resonance energy transfer /Baugh, Jeffrey Loren. January 2003 (has links)
Thesis (Ph. D.)--University of Washington, 2003. / Vita. Includes bibliographical references (leaves 71-79).
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Transcriptioal [sic] and post-transcriptional regulation of extracellular enzyme production in Erwinia carotovora subsp. Carotovora /Liu, Yang, January 2000 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.
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Transcriptioal [sic] and post-transcriptional regulation of extracellular enzyme production in Erwinia carotovora subsp. CarotovoraLiu, Yang, January 2000 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.
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Spectroscopic studies of apolipoprotein e and the low-density lipoprotein receptor /Clayton, Daniel John. January 2001 (has links) (PDF)
Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.
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A morphological, histochemical and experimental study of the prostate gland and seminal vesicles of the guinea pig, with special reference to the stroma /Chan Leung, Franky. January 1989 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1989.
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Einfluss von modifizierter extrazellulärer Matrix auf die Proteinexpression von FibroblastenFreiin von Feilitzsch, Margarete 30 June 2015 (has links) (PDF)
Der humanen dermalen Wundheilung liegt ein komplexes Zusammenspiel verschiedener Faktoren zugrunde. Die Bedeutung dieses fein regulierten Gleichgewichts wird deutlich, wenn es durch Fehlregulationen oder Störungen zu chronischen Wundheilungsstörungen oder lokaler Fibrose mit überschießender Narbenbildung kommt. Eine der möglichen Methoden zur Prävention und Behandlung ist die Deckung der Wunde mit einem Hautersatz. Dabei werden zunehmend sogenannte Biomaterialien aus natürlichen Substanzen mit hoher Biokompatibilität und der Möglichkeit zur Interaktion mit dem nativen Gewebe verwendet. In Studien wurde gezeigt, dass vor allem sulfatierte Glykosaminoglykan-Derivate durch die Interaktion ihrer negativ geladenen Sulfatgruppen mit Zytokinen, Wachstumsfaktoren und dermalen Zellen einen positiven Einfluss auf den Wundheilungsprozess haben können. In der vorliegenden Arbeit wurden daher kollagenbasierte artifizielle extrazelluläre Matrizes mit unsulfatierter oder sulfatierter Hyaluronsäure hinsichtlich ihres Einflusses auf humane dermale Fibroblasten als Komponenten der Wundheilung untersucht. Dermale Fibroblasten spielen im Ablauf der Wundheilung eine tragende Rolle und interagieren eng mit der umgebenden Matrix. Anhand ihrer Proteinexpression lassen sich Rückschlüsse auf wichtige Funktionen wie Adhäsion, Proliferation, Differenzierung und Matrixsynthese ziehen. In den durchgeführten Experimenten zeigte sich, dass sulfatierte Matrix in der Kultur mit dermalen Fibroblasten kein entzündliches Milieu förderte. Die Proliferation, Differenzierung und Migration der Fibroblasten schienen gesteigert, während sich die Matrix-Synthese und ihr Remodeling weder pathologisch gehemmt noch überschießend zeigten. Daher wäre die weitere Untersuchung dieses Biomaterials ein vielversprechender Ansatz, um langfristig dem Risiko von Wundheilungsstörungen wie chronischen Wunden oder fibroproliferativen Wundheilungsstörungen effektiv entgegenzuwirken.
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Type IIA procollagen and the regulation of nodal signalingGao, Yuan, Gene., 高远. January 2011 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Functional analyses of type IIA procollagen in embryo developmentLeung, Wai-lun, Alan., 梁瑋倫. January 2006 (has links)
Type II collagen is the major extracellular matrix (ECM) protein present in
cartilage and is detected in non-chondrogenic tissues such as the heart and the
neural tube during developmental stages involving rapid tissue morphogenesis
indicating an active role played by the collagen in embryogenesis. Type II
collagen is synthesized as a procollagen precursor which has amino- and
carboxyl-terminal globular extensions (N- and C-propeptides) flanking a central
triple helical domain. Two isoforms of type II procollagen are generated by
alternative mRNA splicing of the exon 2: IIA and IIB. Sequence present in the
N-propeptide of IIA, translated from the spliced-in exon 2, encodes a von
Willebrand factor-like C cysteine rich (CR) domain. This domain is homologous
to those present in regulators of the bone morphogenetic protein (BMP) signaling
such as chordin (Chd), twisted gastrulation (Tsg) and crossveinless (Cv).
Previous in vitro binding assays and overexpression studies in frog embryo
suggest that the CR domain of IIA antagonized BMP signaling. In order to give
a better understanding of the function of IIA in embryonic development and
cellular signaling, several approaches including expression pattern analyses,
phenotypic analyses of null mutant and gain of function studies are employed in
this study. Expression studies of IIA mRNA in early postimplantation mouse
embryos find that it is present in the axial mesendoderm (including the anterior
definitive endoderm [ADE] and the prechordal plate) which is a critical head
organizer at neural plate (E7.5) and head process (E8.0) stages. Characterization
of the IIA deficient mice (IIA-/-), constructed by removing exon 2 from type II
collagen (Col2a1) gene by homologous recombination, indeed reveals that the
anterior-most neural tissue is deficient at early somitogenesis denoted by
reduction/loss of the forebrain/optic cup markers. Marker studies indicate that
the ADE may already be affected at the neural plate stage in IIA-/-. The neural
phenotype of IIA-/- displays significant similarities with mutants deficient in BMP
pathway components such as Chd-/-;Nog+/-, Tsg-/- and Tsg-/-;BMP4+/- suggesting
that IIA plays a role in maintaining the specification and/or regulating the
signaling properties of the anterior midline tissue which involves regulation of
BMP signaling. Results of ectopic expression of IIA in Xenopus laevis embryos
suggest that IIA regulate BMP and the related Nodal signaling pathways in a
context dependent manner which has significant implications in normal anterior
neural plate development. Based on the work described in this thesis and the
body of existing evidence, a model is presented which suggests that IIA
promote/maintain anterior neural plate development by regulating the range and
extent of BMP signaling in the anterior neural plate. This study sheds light on
the role of an ECM component in regulating tissue patterning and cellular
signaling during early mouse development and also provides putative function for
the CR domain of other fibrillar procollagens including type I, III and V which is
poorly understood currently. This work will provide the framework for the
design of subsequent studies in re-examining the role of these fibrillar
procollagens in embryogenesis. / published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
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Modulation of adult neural plasticity by proteolytic catabolism of lecticansMayer, Joanne 01 June 2007 (has links)
The extracellular environment of the central nervous system (CNS) through which neuritic processes must traverse during development or after injury is complex, and may vary from stabile conditions to a milieu favorable for neural plasticity and growth. The extracellular space in the CNS accounts for about 20% of brain volume and is composed of aggregating complexes of several different extracellular matrix (ECM) molecules. The ECM supports neural networks and acts as a barrier for neurite extention, depending on the type of molecules involved and the various signals they induce. One mechansim that may produce an environment favoring plasticity is the proteolytic cleavage of ECM. Brevican belongs to the lectican family of aggregating, chondroitin sulfate-containing proteoglycans (CSPGs) and is abundant in brain ECM complexes. It is localized peri-synaptically, inhibits neurite outgrowth, and is thought to stabilize synaptic networks in the adult.
Interestingly, a significant proportion of brevican in the CNS is observed as a fragment of the protein core formed by proteolytic cleavage. Endogenous matrix-degrading proteinases, such as the MMPs (matrix metalloproteinases) and ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs), cleave brevican and other lecticans potentially promoting neural plasticity. Cleavage of brevican and similar lectican family members may "loosen" the aggregated complexes and change the extracellular environment to one that is more permissive toward neural plasticity. After injury, during inflammation or with disease, alterations in the ECM may influence development and/or progression of neurological disease.
The purpose of these studies was to investigate the catabolism of brevican in the ECM and its potential role in neural plasticity under each of these influences, taking an in depth look at how brevican is processed after (1) undergoing a classical model of neural plasticity, the entorhinal cortex lesion (ECL); (2) a disease state that is thought to have dysregulated neural and synaptic plasticity; and (3) how brevican catabolism and neural plasticity is effected by deleting the protease responsible for the cleavage of lecticans in a mouse model. Overall, these experiments provide evidence that the proteolytic cleavage of brevican, and lecticans in general, may play an important role in the regulation of neural plasticity.
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