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In the presented work the relationship between transcription factors and male gametophyte development was studied. The Ph.D. Thesis covers selection of candidate genes, wide-scale screening of T-DNA mutant lines and detailed analysis of a selected transcription factor on pollen development.

Inzulinu podobné a jiné růstové faktory a nádory Insulin like and other growth factors and tumors Disertační práce Plzeň 2011 Mgr. Radek Kučera Summary The interaction between growth factors and cancer incidence and development has been discussed recently. First works suggesting possible connection between increased production of insulin like growth factors with an increased incidence of tumors came already from early 80s of the last century. The aim of my work was focused on evaluation of possible relation between insulin like growth factors or their binding proteins and tumors from different perspectives, to assess their significance and try to determine what role the IGFs and IGFBPs may play in the current tumor diagnostics. The work itself is divided into two parts, the theoretical and practical ones. In the theoretical part, I summarized the action of IGF in human body and also focused on previous findings on the role of IGF system in cancer diagnoses. In the practical part, divided into three subsections, I focused on investigation of relations in a large set of patients with different cancer diagnoses, in a group of women with breast cancer and, finally, I focused on changes of IGF1 levels during follow-up. Theoretical part Insulin-like growth factors (IGF) are peptides, that participate on growth...

The goal of the work is considering strategical options of possible development of Tambor brewery with usage of available quantitative methods of system analysis. The work is execution of agreement of given object and University of Life Sciences in Prague.

The aim of this study was to evaluate the effectiveness of selected investment project to assess the impact of the project on the profitability and financial stability podniku.Diplomová work deals with the specific investment objective, which is the purchase of machinery. Based on the financial analysis is to assess the situation. This is done with the help of indicators of profitability, activity, leverage and liquidity. Then, the paper deals with clarifying the cash flows selected machinery, which are capital expenditures, income and expenses arising from the investment. Furthermore, there is a proper evaluation of the investment project. In conclusion, the assessed risk of the project.

This diploma work takes and describes for the main objective the evolution and the formation of mental maps. These mental maps join with the idea of a travelling and with a travel tourism. The partial objective is the project for an exploitation of these knowledges for the travel tourism. This research is specialized in the basic school students where is the evolution the most descriptive. The evolution of the mental maps is assumed by students of secondary schools and by older informants. The evolution of these mental maps confirms the anylysis of questionnaires.

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Dieser Beitrag gibt einen Überblick über ökonomisch und ökologisch orientierte Messansätze des nachhaltigen Wohlstands. Besondere Aufmerksamkeit wird dabei dem Bruttoindlandsprodukt (BIP) und dem Index of Sustainable Economic Welfare gewidmet. Die Debatte um die Folgeschäden von Wachstumsprozessen hat gezeigt, dass das BIP ein unzulänglicher Wohlstandsindikator ist, der systematisch verzerrte Informationen über die Nachhaltigkeit der Wohlstandsentwicklung liefert. Trotzdem sind bisher keine auf ökologische und soziale Nachhaltigkeit ausgerichteten Indikatorensysteme implementiert, um das BIP als zentralen Wohlstandsindikator zu ersetzen. Die Diskussion in der Wirtschaftswissenschaft und ökologisch orientierte Debatten scheinen sich eher auseinander entwickelt zu haben.

Bordetella pertussis is a strictly human pathogen and causative agent of infectious respiratory disease called whooping cough. In order to establish successful infection and colonization of the host, B. pertussis uses a broad spectrum of virulence factors such as adhesins (filamentous hemagglutinin, pertactin, and fimbriae) and toxins (adenylate cyclase and pertussis toxins). In addition, the type 3 secretion system (T3SS) was also found in the genus Bordetella. In connection to our previous characterisation of B. pertussis strain lacking the gene encoding RNA chaperone Hfq (Δhfq), which proved that Hfq is required for T3SS functionality, the recombinant T3SS proteins BopB, BopD, BopC and BopN were purified to homogeneity. Next, the specific antibodies were obtained using purified recombinant proteins in order to study the production of the T3SS components in B. pertussis. Using refined anti- BopC antibodies it was for the first time shown that laboratory-adapted B. pertussis strain secretes BopC protein into medium. The recombinant translocators BopB and BopD were also used to examine their pore-forming activity using planar black lipid membranes. Based on the characterisation of hfq deletion mutant, having impaired production of membrane proteins when compared to the wild type, mass spectrometry...

The ability of humans to discriminate enantiomeric odour pairs is substance –specific. Current literature suggests that psychophysical discrimination of odour enantiomers mainly depends on the peripheral processing at the level of the olfactory sensory neurons (OSN). To study the influence of central processing in discrimination, we investigated differences in the electrophysiological responses to psychophysically indistinguishable (+)- and (−)- rose oxide enantiomers at peripheral and central-nervous levels in humans. We recorded the electro-olfactogram (EOG) from the olfactory epithelium and the EEG-derived olfactory event-related potentials (OERP). Results from a psychophysical three alternative forced choice test indicated indistinguishability of the two odour enantiomers. In a total of 19 young participants EOG could be recorded in 74 and OERP in 95% of subjects. Significantly different EOG amplitudes and latencies were recorded in response to the 2 stimuli. However, no such differences in amplitude or latency emerged for the OERP. In conclusion, although the pair of enantiomer could be discriminated at a peripheral level this did not lead to a central-nervous/cognitive differentiation of the two stimuli.