Three experiments were conducted to explore the effect of fescue toxicosis on vasoconstriction and various parameters of the estrous cycle. In the experiment of Chapter 3, a 2x2 crossover experimental design with repeated measures was implemented to test whether the ingestion of endophyte-infected tall fescue seed would alter blood hormone concentrations of prolactin, progesterone, and estradiol, interovulatory intervals, and corpus luteum blood flow. Also, Doppler ultrasonography was used to assess whether palmar artery and palmar vein lumen diameter, area, circumference, and resistivity index could be altered by the ingestion of endophyte-infected tall fescue seed. Ingestion of endophyte-infected tall fescue seed significantly decreased palmar artery resistivity index, area, and circumference, and palmar artery and palmar vein lumen diameter, but did not alter interovulatory intervals, serum concentrations of prolactin, progesterone, estradiol, or corpus luteum blood flow. Experiments 1 and 2 from Chapter 4 profiled the dopaminergic, serotonergic, and α-adrenergic receptor types and subtypes, as expressed within the equine medial palmar artery, medial palmar vein, and uterine artery transcriptomes. Combined results from Chapters 3 & 4 imply that relative quantity of serotonergic receptor subtypes within a vessel may be related to with the severity of that vessel's vasoconstrictive response to endophyte-infected tall fescue.

Ingestion of endophyte infected (E+) fescue by pregnant mares can cause significant reproductive problems. Plateau® and Cimarron® herbicides suppress fescue while leaving desired forages unharmed. To determine if these herbicides are harmful to pregnant mares, they were allowed to graze pastures treated with Plateau®, Cimarron®, or vehicle carrier. Pregnancies were monitored via ultrasonography, blood chemistry, and hematology. Of the components measured only creatinine differed among treatments over time (P=0.0003) and that increase was only significant in one of four studies. Two additional experiments were conducted to determine the effect of the herbicides on alkaloids within E+ fescue. A greenhouse experiment utilizing 52 pots of E+ fescue treated with Plateau®, Cimarron®, or nothing was inconclusive, as some alkaloids increased while others decreased. These results indicated that UV light may be required for normal plant death. In a field experiment 12 plots of mixed vegetation were sprayed with the same treatments, and herbicides decreased ergovaline, N-formylloline, and lysergic acid content (P=0.0460, P=0.0324, P=0.0093 respectively). In conclusion, the herbicides did not alter blood components outside physiological norms, but the alkaloids were still present in dying E+ fescue. It may be safest to remove late gestation mares until E+ fescue is completely decayed.

Understanding the nutrient dynamics of pastures is essential to their profitable and sustainable management.  Tall fescue [Schendonorus phoenix (Scop.) Holub.] is the predominant forage species in Virginia pasturelands.  Although tall fescue pasture is common, little research has attempted to document how soil and herbage nutrient concentrations change through time.  This thesis summarizes two studies conducted within the context of a larger grazing systems project near Steele's Tavern, VA.  The objectives were to: (1) examine temporal changes in plant available soil nutrient concentrations in four grazing systems, (2) determine how hay feeding and use of improved forages affected soil and herbage nutrient concentrations (3) examine the relationship between and variability within soil and herbage nutrient concentrations, (4) analyze the seasonal variation in herbage mineral concentration with regard to beef cattle requirements, and (5) create a statistical model to predict variation in herbage mineral concentration across the growing season.  Analysis of plant and soil nutrients through 5 years of grazing produced several important findings.  Soil pH, P, and Ca, Mg, and B declined through five years of grazing.  Higher concentrations of herbage N and K and soil P, K, Fe, Zn, and Cu were measured in hay feeding paddocks.  Herbage nutrient concentrations showed less variability in P and K than did soil test results.  Fertility testing in pastures is important to monitor changing nutrient concentrations, and this study showed that herbage analysis may provide a more stable and accurate assessment of pasture fertility than soil testing.  Pasture herbage, grown without fertilization, contained sufficient concentrations of macronutrients to meet the requirements of dry beef cows through the growing season and to meet the requirements of lactating beef cows in April.  A model was developed using soil moisture and relative humidity that predicted (R2 = 0.75) variation in herbage mineral concentration throughout the growing season.  As described in this thesis, use of modeling to predict nutrient dynamics in pasture could allow for more efficient mineral supplementation strategies that lead to improved profitability, nutrient retention, and livestock health. / Master of Science

Thesis (Ph. D.)--University of Kentucky, 2008. / Title from document title page (viewed on May 12, 2008). Document formatted into pages; contains: xvi, 221 p. : ill. (some col.). Includes abstract and vita. Includes bibliographical references (p. 214-219).

Thesis (M. Agr. Sc.) -- Lincoln University, 2008. / Also available via the World Wide Web.

Thesis (M.S.)--University of Tennessee, Knoxville, 2004. / Title from title page screen (viewed Oct. 04, 2004). Thesis advisor: F. Neal Schrick. Document formatted into pages (xiii,105 p. : ill.). Vita. Includes bibliographical references (p. 86-104).

The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on Feb. 11, 2010). Thesis advisor: Dr. Robert L. Kallenbach. Vita. Includes bibliographical references.

Thesis (Ph. D.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains xi, 95 p. : ill. Includes abstract. Includes bibliographical references (p. 68-76).

Thesis (Ph. D.)--West Virginia University, 2002. / Title from document title page. Document formatted into pages; contains x, 149 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 130-149).

Thesis (M.S.)--Oregon State University, 2008. / Printout. Includes bibliographical references (leaves 73-86). Also available on the World Wide Web.