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Multi-Locus Sequence Typing of Bartonella bacilliformis DNA Performed Directly from Blood of Patients with Oroya's Fever During a Peruvian Outbreak.Pons, Maria J, Silva-Caso, Wilmer, Del Valle Mendoza, Juana, Ruiz, Joaquim 01 February 2016 (has links)
Background
Bartonella bacilliformis is the etiological agent of Carrion’s disease, a neglected tropical
poverty-linked illness. This infection is endemic of Andean regions and it is estimated that
approximately 1.7 million of South Americans are at risk. This bacterium is a fastidious slow
growing microorganism, which is difficult and cumbersome to isolate from clinical sources,
thereby hindering the availability of phylogenetic relationship of clinical samples. The aim of
this study was to perform Multi Locus Sequence Typing of B. bacilliformis directly in blood
from patients diagnosed with Oroya fever during an outbreak in Northern Peru.
Methodology/Principal Findings
DNA extracted among blood samples from patients diagnosed with Oroya’s fever were analyzed
with MLST, with the amplification of 7 genetic loci (ftsZ, flaA, ribC, rnpB, rpoB, bvrR
and groEL) and a phylogenetic analysis of the different Sequence Types (ST) was performed.
A total of 4 different ST were identified. The most frequently found was ST1 present
in 66% of samples. Additionally, two samples presented a new allelic profile, belonging to
new STs (ST 9 and ST 10), which were closely related to ST1.
Conclusions/Significance
The present data demonstrate that B. bacilliformis MLST studies may be possible directly
from blood samples, being a promising approach for epidemiological studies. During the
outbreak the STs of B. bacilliformis were found to be heterogeneous, albeit closely related,
probably reflecting the evolution from a common ancestor colonizing the area. Additional
studies including new samples and areas are needed, in order to obtain better knowledge of
phylogenetic scenario B. bacilliformis
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A meta-analysis of artesunate plus sulfadoxinepyrimethamine alone for treatment of uncomplicated malaria in childrenBenido, Impouma 17 November 2006 (has links)
Faculty of Health Science
School of Public Health
0418444d
benido_impouma@yahoo.com / Study objectives
The objective of this meta-analysis was to review the comparative efficacy and
tolerance of sulfadoxine-pyrimethamine (SP) given alone or in combination with one
(SPAS1) or three (SPAS3) doses of artesunate in children with uncomplicated P.
falciparum malaria, aged 6 months to 10 years. Specifically, it assessed cure rate, fever
and parasite clearance time, gametocyte carriage and tolerability.
Methods
The methodology used was a systematic review and a meta-analysis of four randomised
controlled trials. The primary endpoint was the parasitological cure rate at day 28.
Secondary endpoint included the parasitological cure rate at day 14, time to fever and
parasite clearance, gametocyte carriage and occurrence of adverse events.
Results
Cure rate at day 28 corrected by PCR was 2.5 times higher in the combination of
SPAS3 than in SP alone (pooled OR=2.55, 95% CI 1.93 to 3.37). There was no
difference in cure rate at day 28 corrected by PCR between the combination of SPAS1
and SP alone (pooled OR=1.06 95% CI 0.98 to 1.15). Fever and parasite clearance
times were significantly faster in both SPAS1 and SPAS3 compared to SP alone
(p<0.001). By day 28 all children on the combination therapy were agametocytaemic as
opposed to those on SP alone (p<0.001). All drug regimens were well tolerated and
safe.
Conclusion
The combination of SPAS3 is more efficacious than SP alone in treatment of children
with uncomplicated P. falciparum malaria. The combination is recommended for
adoption as a replacement for SP alone in areas where malaria is endemic.
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Untargeted Lcms Serum Metabolomics Of The Sierra Leonean Lassa Fever Patient And Metaanalysis Of The Virion ProteomeJanuary 2016 (has links)
T V Gale
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Studies on the attenuation of flaviviruses following passage in HeLa cellsDunster, Lee Martin January 1990 (has links)
No description available.
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Immunological correlates of illness severity and course in acute Q feverEverett, Beth Jennifer, Medical Sciences, Faculty of Medicine, UNSW January 2010 (has links)
Acute Q fever is the disease manifestation of Coxiella burnetii infection. This obligate intracellular bacterium is phagocytosed by innate immune cells, where it replicates within the usually bactericidal environment of the phagolysosome. As the immune response is activated, the resultant pro-inflammatory cytokines aid in pathogen clearance but also trigger an acute sickness response in the host. This thesis describes the natural history of acute Q fever in a prospective cohort ??? the Dubbo Infection Outcomes Study (DIOS). In these subjects, the acute febrile illness was characterised by severe headache, drenching sweats and fatigue. In approximately 10% of subjects, symptomatic illness marked by fatigue remained present for months, or occasionally years, after the acute illness. Subjects with more severe acute illness were more likely to develop this post Q fever fatigue syndrome (QFS). The aim of this thesis was to determine whether ongoing infection or aberrant immune activation drive the prolonged symptoms of QFS. Sensitive real time PCR detection of Coxiella DNA revealed a significant minority of subjects had very low copy numbers in circulating monocytes, with an increased prevalence in those with QFS. However, the detection was not consistently found within individual subjects and the copy number was at the threshold of reliable detection. C. burnetii was shown here to stimulate cytokine production in monocytic cells via interaction with Toll-like receptor (TLR)-2 and not TLR-4. Functional polymorphisms in these TLRs were identified in subjects with Q fever, but were not associated with Q fever susceptibility, severity or duration. Phase I-specific responses are believed to be critical in the generation of protective immunity to C. burnetii, yet the phase II-specific responses of innate and adaptive immune components were consistently of higher magnitude. Whole C. burnetii organisms induced antigen-non-specific T cell activation, presumably via the indirect activation of monocytes by C. burnetii LPS. No significant differences were found in the magnitude or kinetics of the host response to infection, or in the carriage of genetic polymorphisms, when comparing subjects who developed QFS with subjects who had promptly resolving illness. It remains unclear what factors mediate the progression of acute Q fever to QFS.
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An inaugural dissertation on the origin and propagation of the yellow fever. : Submitted to the public examination of the faculty of physic under the authority of the trustees of Columbia College, in the state of New-York; the Right Rev. Benjamin Moore, D.D. president; for the degree of doctor of physic, on the 4th of May, 1802. /Bayley, Joseph, Post, Wright, Ledyard, Isaac, Tillary, James, Miller, Edward, January 1802 (has links)
Caption title: An inaugural dissertation on yellow fever. / Dedicated to Dr. Wright Post, professor of anatomy and surgery in Columbia College, and also to Dr. Isaac Ledyard, health officer, Dr. James Tillary, resident physician, and Dr. Edward Miller, health commissioner.
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Disease trend in relation of environmental health a major term report submitted in partial fulfillment ... Master of Public Health ... /Smouse, John P. January 1947 (has links)
Thesis equivalent (M.P.H.)--University of Michigan, 1947.
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Disease trend in relation of environmental health a major term report submitted in partial fulfillment ... Master of Public Health ... /Smouse, John P. January 1947 (has links)
Thesis equivalent (M.P.H.)--University of Michigan, 1947.
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Part I : Isolation and characterization of the highly repetitive sequences from Escherichia coli and their uses in DNA fingerprinting ; Part II :Molecular characterization and initial development of a DNA vaccine against the HOG cholera virus /Wong, Kit-man. January 2000 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 136-146).
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Investigations on parturient paresis a study of levels of certain blood and urinary constituents at the time of parturition in dairy cattle /Blosser, Timothy Hobert, January 1949 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1949. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 113-121).
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