Made available in DSpace on 2014-12-17T14:03:45Z (GMT). No. of bitstreams: 1 MariaLC.pdf: 493490 bytes, checksum: ba60f2812dd358a875dfd794b36dacc3 (MD5) Previous issue date: 2007-06-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The fucoidan from Fucus vesiculosus is known for having diverse biological properties. This study analyzed the therapeutic action of populations of commercial fucoidan (F. vesiculosus) on zymosan-induced arthritis. Three populations of fucoidan were obtained after acetone fractionation; these were denominated F1 (52.3%), F2 (36.7%) and F3 (10.7%). Chemical analyses showed that F1 contained the largest amount of sulfate ion. The electrophoretic profile shows that the commercial or total fucoidan (TF), different from the other fucoidans and from glycosaminoglycan patterns, is quite polydisperse, which indicates that it is composed of a mixture of sulfate polysaccharides. On the other hand, the fucoidans obtained from TF showed only an electrophoretic band with much lower polydispersion than that observed for TF. Fucoidan F2 showed a migration between fucoidans F1 and F3. Owing to the small amount of mass obtained from F3, we used only fucoidans F1 and F2 in the induced arthritis tests. After 1 hour of induction, we administered F1 or F2 (10, 25 and 50 mg/kg i.p.) or diclofenac sodium (10 mg/kg i.p.) or lumiracoxib (5 mg/kg o.a.) or L-NAME (30 mg/kg i.p.). After 6 hours, we performed analyses of cell influx and nitrite levels in addition to histopathological analysis. Fucoidans F1 and F2 were more potent both in decreasing the number of leukocytes and the amount of nitric oxide found in the synovial fluid. This indicates that the anti-inflammatory mechanism of these fucoidans is not only related to selectin block, but also to nitric oxide synthesis inhibition / A fucoidana de Fucus vesiculosus ? conhecida por exibir fun??es biol?gicas diversas. Este trabalho analisou a a??o terap?utica de popula??es obtidas da fucoidana comercial (F. vesiculosus) no modelo de artrite induzida por zymosan. Ap?s fracionamento com acetona obteve-se tr?s popula??es de fucoidana que foram denominadas de fucoidanas F1 (52,3%), F2 (36,7%) e F3 (10,7%). As an?lises qu?micas demonstraram que F1, dentre as fucoidanas obtidas, foi a que apresentou a maior quantidade do ?on sulfato. O perfil eletrofor?tico mostra que a fucoidana comercial ou total (FT), diferente das outras fucoidanas e dos padr?es de glicosaminoglicanos, se mostrou bastante polidisperso, o que indicou que essa ? constitu?da por uma mistura de polissacar?deos sulfatados. Por outro lado, as fucoidanas obtidas a partir de FT mostraram apenas uma banda eletrofor?tica com uma polidispers?o bem menor do que aquela observada para FT. A fucoidana F2 apresentou uma migra??o intermedi?ria entre as fucoidanas F1 e F3. Devido a pequena quantidade de massa obtida de F3, utilizou-se apenas as fucoidanas F1 e F2 nos ensaios da artrite induzida. Ap?s 1 hora da indu??o foram administradas F1 ou F2 (10, 25 e 50 mg/Kg i.p) ou diclofenaco s?dico (10 mg/Kg i.p) ou lumiracoxibe (5 mg/Kg v.o) ou L-NAME (30 mg/Kg i.p). Ap?s 6 h, foram realizadas as an?lises do influxo celular e n?veis de nitrito e a an?lise histopatol?gica. As fucoidanas F1 e F2 foram mais potentes tanto na diminui??o do n?mero de leuc?citos, quanto na quantidade de oxido n?trico encontrado no lavado. O que indica que o mecanismo antiinflamat?rio dessas fucoidanas n?o est? s? relacionado com o bloqueio das selectinas, mas tamb?m com a inibi??o da s?ntese do oxido n?trico