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Gene expression profiling in non-small cell lung cancerLam, Chi-leung, David. January 2007 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
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The isolation and characterization of chicken histone genesHarvey, Richard Paul. January 1982 (has links) (PDF)
No description available.
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Molecular characterization and genetic recombination of snakehead rhabdovirusJohnson, Marc C. 25 February 1999 (has links)
The complete genome of snakehead rhabdovirus (SHRV) was cloned and
molecularly characterized. This was initially accomplished through the sequence
determination of its glycoprotein gene and the phylogenetic analysis of this gene with
orthologous genes from other rhabdoviruses. The phylogenetic analysis revealed that
SHRV groups with viruses of the genus Novirhabdovirus. The full-length glycoprotein
was expressed in mammalian cells to investigate its potential use in the production of
pseudotyped retroviruses.
The sequence of the entire SHRV genome of 11.6 kb was determined, and all
encoded proteins, intergenic transcriptional control motifs, and the leader and trailer
regions were identified. The genome was found to encode six proteins including a
nucleoprotein, a phosphoprotein, a matrix protein, a glycoprotein, a small--presumably
non-virion--protein, and a polymerase protein. The presence of a non-virion protein,
which is the hallmark feature of all Novirhabdoviruses, supported SHRV's identity as a
member of the Novirhabdovirus genus, despite the fact that the non-virion protein
showed no homology with any known protein.
A system was developed to express a full-length, error-free positive-strand copy
of SHRV's RNA genome along with all of the SHRV proteins required for viral
replication within the cytoplasm of a virus-susceptible host cell. These factors
collectively allowed the recovery of live virus entirely from cloned cDNAs. A unique
restriction site was engineered into SHRV's cDNA genome, and the presence of this
restriction site was verified following virus recovery, proving the recovered virus was
indeed a live recombinant virus. To our knowledge this achievement marks the first time
in which reverse genetics has been performed on a nonmammalian negative-stranded
RNA virus. / Graduation date: 1999
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A novel probabilistic framework for microarray data analysis from fundamental probability models to experimental validation /Gelmi, Claudio A. January 2007 (has links)
Thesis (Ph.D.)--University of Delaware, 2006. / Principal faculty advisors: Babatunde Ogunnaike and Jeremy S. Edwards, Dept. of Chemical Engineering. Includes bibliographical references.
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The Genetic Basis of Evolved Differences in Gene Expression in Fundulus heteroclitusScott, Cinda Pitts 26 March 2009 (has links)
This dissertation explores the genetic basis of gene expression in Fundulus heteroclitus by focusing on the role of the environment and its effects on gene expression and by making direct estimates of heritability using cDNA microarrays. The second chapter describes the utility of F. heteroclitus cDNA microarrays for studies of F. heteroclitus which seek to understand the genetic variation in gene expression. Measurements of mRNA fluorescence and concentration as well as differences in sample preparation and sampling of blood from a single individual over time demonstrate that F. heteroclitus cDNA microarrays are quantitative, reproducible and consistent. The third chapter examines the effect of the environment and genetic factors on the variation of gene expression. F. heteroclitus cDNA microarrays are used to determine whether a genetic component of gene expression can describe the variation in gene expression between inbred and outbred individuals from the same population. The results show that variation in mRNA expression is related to the genetic variation among individuals within a group. While chapter three reveals that there is a genetic component of variation in gene expression, the percentage of genes that are significantly heritable was not known. In the fourth chapter, the heritability of the variation in gene expression is estimated to determine the genetic basis of gene expression in F1 individuals from natural, outbred populations of F. heteroclitus. The data presented in chapter 4 are the first to formally estimate the genetic component of gene expression in F. heteroclitus. The estimates of heritability range from 0.25 to 0.86 depending on the estimation method with approximately 6.5% of genes having significant heritability. The results presented in this dissertation support the concept that genetic variation affects variation in mRNA expression among natural populations of F. heteroclitus. Natural, heritable variation in gene expression is important for understanding evolutionary adaptation and the role of natural selection in evolutionary processes.
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Novel cancer therapeutics, the generation of ROS, and cell survival /Mitchell, Clint, January 2006 (has links)
Thesis (Ph. D.)--Virginia Commonwealth University, 2006. / Prepared for: Dept. of Biomedical Engineering. Bibliography: leaves 202-220. Also available online.
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Regulation of dickkopf-1(Dkk-1) promoter sequences under various growth conditionsFeimster, Jasmin Denee. January 1900 (has links) (PDF)
Thesis (M.S.)--University of North Carolina at Greensboro, 2006. / Title from PDF title page screen. Advisor: Karen Katula; submitted to the Dept. of Biology. Includes bibliographical references (p. 59-61).
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Characterization of Sad1/UNC-84 domain protein 2 (SUN2)Liang, Ying, January 2006 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2006. / Title proper from title frame. Also available in printed format.
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Isolation, characterization, and expression analysis of genes encoding starch synthesizing enzymes from grain amaranthLu, Bei. January 2006 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2006. / Title proper from title frame. Also available in printed format.
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Gene expression profiling and modeling of cervical cancerCarlson, Mark Wallace, January 1900 (has links) (PDF)
Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.
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