Thesis (Ph.D.)--University of Adelaide, Dept. of Paediatrics, 2003. / "16th September 2002." Accompanying CD contains 2 MPEG clips with accompanying text, and a copy in PDF format of: Recovery of airway cystic fibrosis transmembrane conductance regulator function in mice with cystic fibrosis after single-dose lentivirus-mediated gene transfer / M. Limberis ... [et al.], published in Human gene therapy vol. 13 (2002). Bibliography: leaves xxix-li.

Undergraduate honors paper--Mount Holyoke College, 2009. Dept. Program in Neuroscience and Behavior. / Includes bibliographical references (leaves 90-96).

Thesis (Ph. D.)--Chemical and Biomolecular Engineering, Georgia Institute of Technology, 2006. / Harish Radhakrishna, Committee Member ; Mark Prausnitz, Committee Co-Chair ; Joseph Le Doux, Committee Chair ; Timothy Wick, Committee Member ; Richard Compans, Committee Member ; Athanassios Sambanis, Committee Member.

Thesis (Ph.D.)--University of Missouri-Columbia, 2005. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (November 27, 2006) Vita. Includes bibliographical references.

Thesis (M.S.)--University of Nevada, Reno, 2008. / "December, 2008." Includes bibliographical references (leaves 53-59). Online version available on the World Wide Web.

The history of life has involved several major evolutionary transitions that have each led to the emergence of a new individual. Examples of major transitions in individuality include the evolution of the eukaryotic cell, multicellular organisms and eusocial societies. In each of these events, previously independently replicating units (cells, individuals etc.) cooperate to form a new individual, which can then only replicate as a whole. For this to occur, conflict between individuals needs to be minimised, to allow maximising their inclusive fitness to be roughly equivalent to maximising group fitness. It has been predicted that the way in which social groups form should be key for eliminating conflict between individuals and promoting cooperation. In this thesis, I have focused on two major evolutionary transitions; the evolution of multicellularity and the evolution of symbiosis, and show that the mode of group formation (whether groups are parentoffspring associations or not) is crucial for understanding when and why major transitions occur. Firstly, I show that the major transition to obligate multicellularity has only occurred with clonal group formation (where cells remain together after division). Secondly, I use an experimental system to show that predation pressure may be key in promoting the formation of multicellular groups in algae. Finally, I show that the mode of group formation is also important in between-species transitions. I use the evolution of symbiosis to show that transmission route of symbionts and environmental factors, determine how cooperative symbionts will be towards their hosts.

No description available.

Cell division was obtained from cultured microspores of Douglas-fir on medium supplemented with auxin, cytokinin, and sucrose, but without medium salts. Embryogenic callus was initiated from excised mature and immature zygotic embryos of Douglas-fir on media supplemented with cytokinin and auxin. Precotyledonary embryos produced most of the embryogenic calli in the cultures. Secondary embryogenic callus production, and subsequent subculturing, were required for the establishment of stable embryogenic callus lines for both mature and immature zygotic embryos. Somatic embryos at the precotyledonary stage were obtained in high frequency when Douglas-fir embryogenic callus was transferred onto hormone-free medium supplemented with 1% activated charcoal, while some cotyledonary somatic embryos were obtained from hormone-free medium supplemented with low ABA levels (0-10 uM). The level of ABA in the maturation medium significantly affected the quality of the somatic embryos produced. Cell suspensions were established from embryogenic calli and have been maintained for over one year. Protoplasts were isolated from suspension, cell colonies and calli were regenerated from protoplasts. GUS and CAT genes were successfully introduced into protoplasts of Douglas-fir via electroporation, and their transient expression was obtained 2-4 days after electroporation. The results so far indicate that the production of somatic embryos via embryogenesis in vitro is obtainable, and the application of direct gene transfer via electroporation for genetic engineering of trees in this species is promising. / Forestry, Faculty of / Graduate

The purpose of this study was two-fold. The first objective was to determine if Saccharomyces cerevisiae is a useful system for investigating the expression of T-DNA (it takes several months to obtain sufficient bacteria-free transformed plant tissue to investigate T-DNA transcription). A short fragment of T-DNA carrying T-DNA gene 7 was cloned into a yeast plasmid in an attempt to investigate the expression of gene 7 in yeast. The second objective was to determine the significance of a heat shock related sequence identified in the 5¹ region of T-DNA gene 7. Primer extension analysis, SI nuclease mapping, and Northern hybridizations indicate that transcription of T-DNA gene 7 in yeast is different from that of transcription of gene 7 in crown gall tumors. Transcription is different because the distance between the TATA box and the transcription initiation sites must be at least 40 nucleotides in yeast. Therefore, Saccharomyces cerevisiae does not appear to be a useful system for investigating the expression of T-DNA. Crown gall tumors were subjected to a number of stress agents, including heat shock, to determine the significance of the heat shock related sequence identified in gene 7. Primer extension analyses indicate that only cadmium and mercury have a significant effect on the expression of T-DNA gene 7. Although gene 7 responds to cadmium and mercury, the increase in transcription does not appear to be heat shock or metallothionein related, indicating that another mechanism is involved in the enhanced transcription of T-DNA gene 7 in crown gall tumors. / Medicine, Faculty of / Medical Genetics, Department of / Graduate

An Agrobacterium-mediated transformation system of purple raspberry ‘Amethyst’ was established after a series of experiments that determined the effect of genotype, inoculum density, and co-cultivation time on transformation. In this study, a plant regeneration protocol was established for ‘Joan J’ and ‘Polana’ (the regeneration protocol of ‘Amethyst’ was previously developed). Agrobacterium-mediated transformation was conducted for all three cultivars. The minimum killing level of hygromycin B and kanamycin was determined. Inoculum density and co-cultivation time were optimized. Polymerase chain reaction (PCR) verified a successful transformation of ‘Amethyst’ with the frequency of 3.3 ~ 4.4 % when leaves were infected with Agrobacterium EHA105 at the cell density of OD600 0.3 and co-cultivated for 3 days in the medium with 25.0 mg∙l-1 kanamycin. Transgenic lines with the PtFIT gene were hydroponically grown under iron sufficiency or deficiency. The real-time quantitative PCR verified the gene expression in response to iron sufficiency and deficiency conditions.