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Immunoassays or LC-MS/MS? : A Comparison Revealing the Properties of Modern Methods for Insulin, Pro-insulin, C-peptide and Glucagon QuantificationUpite, Ruta, Wärmegård, Susanna, Tiger, Casper, Ivert Nordén, Anna, Martinez, Temis, Umenius, Viktor January 2019 (has links)
The purpose of this report is to compare seven different methods for biomarker detection and quantification based on previously published papers. The methods investigated are ELISA, LC-MS/MS, UPLC-MS/MS, LC-IM/MS, IA-LC-MS/MS, MSIA-HR/AM, HTRF and AlphaLISA ® . The focus lies on biomarkers relevant for diabetes, obesity and cardiovascular diseases.Namely insulin, proinsulin, glucagon and C-peptide. Particular significance is assigned to the comparison of the currently widest used method, ELISA, with various types of LC-MS/MS. The report concludes ELISA being superior to LC-MS/MS methods in terms of recovery and precision, while LC-MS/MS is superior in accuracy, multiplexing, specificity, throughput and sample cost. This suggests that different types of LC-MS/MS has the potential to gain momentum in the field of biomarker quantification if they become more available.
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