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Reactions of muconaldehyde with biomoleculesDelaney, Kirsty January 1998 (has links)
No description available.
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The toxicity of methyl iodide : in vivo and in vitro mechanistic studies in the rat nasal cavity and cerebellumChamberlain, Mark Peter January 1998 (has links)
No description available.
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The ligand-binding function of the porcine class Pi glutathione S-transferaseBico, Paula C G 20 July 2016 (has links)
A dissertation submitted in fulfilment of the requirements for the degree of Master of Science
at the University of the Witwatersrand.
Johannesburg
February 1994 / Glutathione S-transferases are multifunctional intracellular proteins. They catalyse the
conjugation of glutathione to endogenous'or foreign electrophiles, and also bind non-substrate
ligands.
Class Pi glutathione S-transferase (pGSTPl~l) was purified from porcine lung to a specific.
activity of 6.63p.ffiol/min/mg. The homodimeric protein has a molecular weight of about
4~.7kD and an isoelectric point of 8.6.
Anionic ligand-binding properties of this isoenzyme were investigated. Steady-state
fluorescence methods were used to determine ~ values for 8-anilino··l~naphtha1enesulphonic
acid (K, == 17.1p.M and 11.1J.tM using fluorescence enhancement techniques and quenching
techniques respectively), bromosulphophtbalein (Kcl=1.1p.M at pH 6.5 and 2.4/jM at pH
7.5) and glutathione {~=1201I.M). The affinity of bromosulphophthalein for the enzyme,
in the presence of 10mM glutathione was slightly enhanced (~=O.7.uM at pH 6.5). The
energy transfer betwecz the protein's tryptophan residues and 8-anUino-l-naphthalene
sulphonic acid was observed and found to be about 56% efficient. The impact of ligand
binding on both protein structure and catalytic activity were assessed. Kinetic studies show
that the active site of the enzyme is not the primary binding site for the non-substrate ligands,
but that the binding of bromosulphophthalein and to a lesser extent 8~ani1ino-l-!.~phtha1ene
sulphonic acid, does affect the active site of the enzyme, especially aner saturating
concentrations of the ligand. This may be the result of a small ligand-induced conformational
change. Fluorescence studies also indicate that the primary site for anionic ligand binding
is not in close proximity to either Trp28 or Trp38 in domain I, Competition studies indicated
that the two anionic ligands bind the Same site, < Prorein fluorescence, chemical modification
«
and size-exclusion HPLC data indicate that ligand binding does 110t induce gross
conformational changes in the protein.
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Arsenic tolerance in mesquite (Prosopis sp.): Low molecular weight thiols synthesis and glutathione activity in response to arsenicMokgalaka-Matlala, NS, Flores-Tavizon, E, Castillo-Michel, H, Peralta-Vdea, JR, Gardea-Torresdey, JL 02 July 2009 (has links)
a b s t r a c t
The effects of arsenic stress on the production of low molecular weight thiols (LMWT), glutathione
S-transferase activity (GST) and sulfur metabolism of mesquite plant (Prosopis sp.) were examined in
hydroponic culture at different arsenic [As(III) and (V)] concentrations. The production of LMWT was
dependent on As speciation and concentration in the growth medium. The roots of As(III) treated plants
produced significantly higher LMWT levels than As(V) treated roots at the same concentration of
As applied. In leaves, the thiols content increased with increasing As(III) and (V) concentrations in the
medium. Hypersensitivity of the plant to high As concentrations was observed by a significant decrease
of LMWT produced in the roots at 50 mg/L treatment in both As(III) and (V) treatments. Sulfur was
translocated from roots and accumulated mainly in the shoots. In response to As-induced phytotoxicity,
the plants slightly increased the sulfur content in the roots at the highest As treatment. Compared with
As(V)-treated plants, As(III)-treated roots and leaves showed significantly higher GST activity. The roots
of both As(III) and (V) treated plants showed an initial increase in GST at low As concentration (5 mg/L),
followed by significant inhibition up to 50 mg/L. The leaves had the highest GST activity, an indication of
the ability of the plant to detoxify As in the leaves than in the roots. The correlation between LMWT
content, S content and GST activity may be an indication these parameters may be used as biomarkers of
As stress in mesquite.
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Alpha-class glutathione transferases as steroid isomerases and scaffolds for protein redesign /Pettersson, Pär L. January 2002 (has links)
Diss. (sammanfattning) Uppsala : Univ., 2002. / Härtill 4 uppsatser.
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Structural studies of redox homeostasis enzymesBiterova, Ekaterina I. January 2009 (has links)
Thesis (Ph.D.)--University of Nebraska-Lincoln, 2009. / Title from title screen (site viewed June 26, 2009). PDF text: viii, 99 p. : ill. (some col.) ; 22.5 Mb. UMI publication number: AAT 3350441. Includes bibliographical references. Also available in microfilm and microfiche formats.
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Application of ionspray tandem mass spectrometry to mechanistic studies on the bioactivation of xenobiotics /Jin, Lixia, January 1996 (has links)
Thesis (Ph. D.)--University of Washington, 1996. / Vita. Includes bibliographical references (leaves 162-186).
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Mechanistic studies on the electrochemistry of glutathione and homocysteineOyesanya, Olufemi. January 1900 (has links)
Thesis (Ph.D.)--Virginia Commonwealth University, 2008. / Prepared for: Dept. of Chemistry. Title from thesis description page. Includes bibliographical references.
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Microsomal glutathione transferaseMorgenstern, Ralf. January 1983 (has links)
Thesis (doctoral)--University of Stockholm, 1983. / Publications on which thesis is based are appended. Includes bibliographical references.
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Glutathione transferase M1-1 delineation of xenobiotic substrate sites and the relationship between enzyme structure and catalytic function /Hearne, Jennifer L. January 2006 (has links)
Thesis (Ph.D.)--University of Delaware, 2006. / Principal faculty advisor: Roberta F. Colman, Dept. of Chemistry and Biochemistry. Includes bibliographical references.
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