Dosage du polyisoprène et des résines de la biomasse de guayule (Parthenium argentatum) par spectroscopie proche infrarouge (SPIR) Méthodes d'extraction par solvant de référence Un protocole basé sur l'extraction accélérée (ASE) avec l'acétone (EA) (résine) puis l'hexane (EH) (polyisoprène, PI) a été sélectionné et optimisé (rendement maximal; adapté à l'analyse de séries) ; quantification d'abord basée sur le poids de l'extrait (gravimétrie). L'EH est maximal à 120°C après étapes avec l'acétone à 40°C (plan d'expérience). La contamination croisée a été confirmée par SEC et FTIR (5 à 29%), conduisant à une deuxième méthode basée sur résine et PI et non plus sur EA et EH, incluant le PI de faible masse molaire (Mw) de l'EA. Ces 2 méthodes de référence ont servi à calibrer la SPIR (chimiométrie/PLS, coeff. beta) afin de relier signature spectrale, PI, résine. ASE-SPIR, couplés ici pour la première fois, ont été plus performants (R² 0.96; 0.98; RPD 4.8; 4.6; EA et EH resp.) que les méthodes de la littérature, grâce aux 215 échantillons représentatifs (génotypes, saison, âge du guayule, climat). La méthode tenant compte de la contamination est moins performante (erreur exp. due aux analyses SEC et FTIR; variation de composition des résines). Ayant montré la dégradation du PI au cours de l'extraction, un autre protocole a été étudié (biomasse humide, un solvant, une seule étape, 20°C) afin d'accéder au Mw «natif », donnant 2.106 g au lieu de 6.105 avec la biomasse séchée; il faut donc être prudent face aux Mw de guayule de la littérature. Ce travail montre la nécessité de tenir compte de la complexité de la biomasse de guayule (échelle cellulaire/PI vacuolaire, résine des canaux; moléculaire/instabilité chimique) lors de l'extraction du PI pour l'analyse structurale. Ces méthodes analytiques ont contribué à produire des prototypes (gant non allergisant, pneu) à haut Mw et à l'acclimatation en Europe dans le cadre du projet EU-Pearls. / Measurement of resin and polyisoprene in Parthenium argentatum (guayule) biomass using near infrared spectroscopy (NIRS) -Associated solvent-based reference methods .A protocol based on sequential extraction with acetone (resin) and hexane (polyisoprene, PI) with accelerated solvent extraction (ASE) was optimized and selected (maximized yield; adapted to large series) instead of Soxhlet and homogenizer. Quantification was first based on extract weight (gravimetry). Hexane extract was maximized at 120°C, after acetone steps at 40°C, through an experimental design. Cross contamination was confirmed and quantified (5 to 29%; SEC and FTIR). This gave a second method based on resin and PI, instead of crude extracts, accounting for low average molar mass PI (Mw) extracted by acetone instead of hexane. Both reference methods were used for calibrating NIRS applied to powdered biomass, with chemometric tools (PLS loadings, beta coefficients) to interpret spectral bands vs PI-resin relationship. ASE, not used before as reference, is highly reliable, and calibration with gravimetry (R² 0.96; 0.98; RPD 4.8; 4.6; for acetone and hexane extract) better than published data, thanks to the 215 samples covering genotypes, harvest date, plant age, climate. The method using cross contamination was less efficient because of higher experimental error induced by additional SEC and FTIR, and change in resin composition. Having set NIRS methods, a new protocol (single solvent THF, minimized processing, 20°C, fresh biomass) was designed to avoid degradation, yielding PI extracts with Mw above 2.106g/mole, closer to in vivo structure (6.105 when using dried guayule); caution to sample preparation in literature dealing with guayule PI structure. This calls for considering the complex structure of guayule biomass (PI in cells; resin in ducts; chemical instability) when extracting PI. These methods allowed producing high PI Mw glove and tire prototypes and domesticating this new crop in Europe within the EU-Pearls project.

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Conducted under USDA Agreement No. 58-3159-7-11 Guayule Natural Rubber Program / Introduction: The United States imports 100 percent of its natural rubber. Ninety-two percent of our natural rubber production is concentrated in Southeast Asia, which is subject to interruptions through political action or direct military intervention. Natural rubber, a critical and strategic material, is necessary in such articles as aircraft and ground vehicle tires, medical supplies, resilient mounts, and certain acoustical applications. Synthetic elastomers cannot meet performance requirements in these areas. A domestic source of natural rubber will assist in assuring a supply of this critical material for industry and defense. The U.S. Department of Defense (DoD) and the U.S. Department of Agriculture (USDA) signed a Master Memorandum of Understanding in 1982 that calls for cooperation with respect to food, agriculture, forestry, nutrition, and other research of mutual interest. With this MOU both agencies adopted a supplemental agreement in 1986 that initiated the Joint Guayule Domestic Rubber Program, whose ultimate goal is to promote an economically viable domestic guayule rubber industry. To accomplish this goal, both agencies have provided funding to plant and cultivate guayule shrubs, construct a 150 -long- ton -per -year prototype plant to extract rubber from the shrubs, and conduct evaluations to establish the performance capability of military products fabricated with domestic guayule rubber. A critical component of the supplemental agreement calls for the USDA to assess the feasibility of a commercial guayule rubber processing facility of 50,000-long-ton-per-year nameplace capacity. This report, based on the best available data (1990) and on assumptions of future advancements in technology (for 1996), is designed to address the commercial prospects for the establishment of a domestic guayule rubber industry. It also examines the feasibility and factors involved in meeting either 20 percent or 100 percent of the natural rubber needs of the U.S. Department of Defense in both peacetime and national emergency conditions.

abstract: Natural rubber and rubber products can be produced from the guayule plant (Parthenium argentatum Gray), which is a low input perennial shrub native to Mexico and the American Southwest. Guayule rubber has the potential to replace Hevea (Hevea brasiliensis) rubber, the most common natural rubber, and synthetic rubber, which is derived from petroleum, in a wide variety of products, including automobile tires. Rubbers make up approximately 47% of the analyzed conventional passenger tire's weight, with 31% from synthetic rubber and 16% from natural Hevea rubber. Replacing the current rubber sources used for the tire industry with guayule rubber could help reduce dependency on imported rubber in addition to reducing greenhouse gas emissions. Moreover, residues from guayule rubber are being researched as a bioenergy feedstock to further improve the environmental footprint of guayule rubber products. This study used life cycle assessment (LCA), a useful tool to determine environmental impacts from a product or process, to quantify and compare environmental impacts of the raw material extraction, transportation and manufacturing of a conventional and a guayule rubber based passenger tire. The impact results of this comparative LCA identified the major environmental impacts and contributing process and informed how the impacts from the tire production can be reduced through utilization of natural rubber co-products as electricity off-sets and reducing guayule rubber's environmental impacts through agricultural and transportation modifications. Results showed that tire raw material extraction contributed the majority of impacts in all categories, where the production of guayule rubber for guayule tires, and the production of synthetic rubber for conventional tires, were the main contributors. Guayule rubber impacts occurred mainly from electricity consumption for agricultural irrigation, while synthetic rubber is a petroleum-based material resulting in high impacts. Transportation impacts had little significance compared to other stages in the life cycle, except for smog impacts, which occurred mainly from truck transport for guayule tires, and transoceanic transport for conventional tires. Tire manufacturing impacts occurred mainly from electricity use in the facilities and were reduced with the use of guayule rubber in guayule tires. / Dissertation/Thesis / M.S. Civil and Environmental Engineering 2014

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Recently, canopy temperature measurements and atmospheric vapor pressure deficits have been used to determine water stress in numerous plant species. A linear regression of the two parameters yields a crop water stress index (CWSI) baseline capable of determining a fraction of water stress between 0 (wet) and 100 (dry). Such a baseline was determined for one line of guayule (N396) in the spring of 1983 and used to determine time of irrigation for field plots. Three irrigation treatments were chosen based on CWSI measurements to aquire a relationship between rubber yield and water Stress. Treatments were irrigated when their respective CWSI measurements reached 0.30 (wet=1), 0.60 (medium=2), and 0.90 (dry=3). Duplicate tests were planted to determine if water delivery by drip irrigation differed from furrow irrigated plots. Very good correlations exist between rubber yield and seasonally averaged CWSI (r = 0.85). The interrelationship of rubber yield and total water applied was also high with r = 0.87. The highest rubber yield occurred in March in treatment 1 (wet) in the furrow irrigated field. Rubber yield was positively correlated with total water applied and inversely correlated with CWSI. Small differences were observed between the type of water delivery system used with similar treatments from both fields not being significantly different. The wet and medium treatments in both fields were about the same with the dry treatment being significantly different from the wet and medium treatments for total water applied. This indicates that guayule is not as sensitive to changes of 0.30 CWSI units as other species measured. Correlations between the CWSI and soil moisture deficits were very good with r=0.83 for 1983 and r=0.91 for 1984 indicating the CWSI can be used accurately to determine soil moisture deficits and vice-versa. A 0-stress moisture deficit range (0-SMDR) was determined for guayule where 0.0-2.26 and 0.0-4.58 cm (1983 and 1984, respectively) of water could be depleted from the soil profile before the plants showed stress as indicated by the CWSI. The 0-SMDR appears to be constant for a particular plant species in the same field for a particular year and may further define and standardize plant available soil moisture.

Philosophiae Doctor - PhD (Biotechnology) / Guayule (Parthenium argentatum) is grown for its high quality hypoallergenic natural rubber latex production. The plant is native to the Chihuahua desert of North America and successful trials have been done in Europe, the United States of America (USA) and the Republic of South Africa (RSA). It is already undergoing industrial scale development in the USA producing good quality rubber products with impressive stretchiness and strength. The performance of guayule lines AZ1, AZ2, AZ3, AZ4, AZ5, AZ6, OSU1, and 11591 was investigated in the Eastern and Western Cape regions in RSA with an ultimate goal of leading to commercial production. To ensure continuous supply of plants, and avoiding documented seed dormancy issues, a micropropagation protocol was established using the OSU1 guayule line. In addition, laboratory scale latex extraction was accomplished using the Waring blender method with KOH pH 11 buffer and the amount of latex was quantified using the 1 ml latex quantification method. The extracted latex homogenate from the different guayule lines was pooled and purified using Sodium Carboxymethyl Cellulose. The efficiency of different molecular weights (90 000, 250 000 and 700 000) of Sodium Carboxymethyl Cellulose was determined in creaming guayule latex at room temperature and 4 degrees celcius. The optimal creaming results were incorporated into creaming latex extracted from the different guayule lines during this study.

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