The existence of fetal microchimerism has been demonstrated many years ago. This phenomenon is associated with observation of two or more genetically different populations of cells present in one person. Fetal microchimerism originates naturally during pregnancy, by bidirectional transfer of the cells through placenta from fetus to mother (fetal microchimerism) and from mother to fetus (maternal microchimerism). In some cases fetal cells persisted in mother for decades after pregnancy. In my thesis I showed the presence of fetal microchimerism in tissues of endometrial cancer, breast cancer and ovarian cancer and in control, nonmalignant tissues. I worked with deep-frozen tissues, native tissues and cell cultures created from native tissues. I planed also the analysis of paraffin-embedded tissues; however this type of material showed to be unusable for fetal cells detection. On the contrary, native and deep-frozen tumor and control tissues are suitable for this type of research and fetal microchimerism was observed in part of samples. For detection and amplification of DNA extracted from tissues and cell cultures I used quantitative real-time PCR and SRY gene located on the Y chromosome as a marker of fetal cells. I detected the presence of male fetal cells. Fetal genome was found in both tumor and...

Backrounds: Usage of addictive substances is a factor which affects the reproductive health of the female users, it affects their risky sexual behavior and fetus healthy development during pregnancy of drug using mothers. Pregnancy also represents a moment in their lifes which affects certain bio-psycho-social aspects forming the beginning of usage of substance abuse. Objectives: The aim of the research was to map a degree and paterns among problem female drug users and changes throughout pregnancy, map the risks of their sexual behavior and quality of their reproductive health, try to find out the availability of gynacology care for female drug users, the amount of care available before, through and after pregnancy. It also includes specific needs and factors affecting reproductive health. Methods and research group: A questionnaire survey of 25 problem female users based on consultations with them, all of them giving birth at least once, was carried out. The study took place at K-center Sananim, using a Field program Progressive, o.s. and around this facility and the place of field program using the "snow ball method". The questionnaire included 164 items in the following parts "Sociodemographic data", "Drug Use", "Risky sexual behavior and reproductive health", "Gynecologic care and barriers to...