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Accumulation of nickel (Ni 2+) by immobilized cells of enterobacter sp.January 1990 (has links)
by Kwok Shu Cheung, Eric. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 89-106. / Acknowledgement --- p.i / Abstract --- p.ii / Introduction / Chapter A. --- Objective --- p.1 / Chapter B. --- Literature Review / Chapter 1. --- Electroplating industry in Hong Kong and its impact on the environment --- p.7 / Chapter 2. --- Physical and chemical methods for removing heavy metals from electroplating effluent --- p.11 / Chapter 3. --- Removal of heavy metals by conventional activated sludge process --- p.16 / Chapter 4. --- Acclimation of wastewater bacteria to heavy metals --- p.26 / Chapter 5. --- Biosorbent and its role in metal detoxification --- p.28 / Materials and Methods / Chapter A. --- Isolation and selection of nickel-resistant bacteria --- p.32 / Chapter B. --- Culture medium and solution --- p.33 / Chapter C. --- Growth of organism --- p.33 / Chapter D. --- Immobilization of bacterial cells --- p.36 / Chapter E. --- Effect of growth conditions on nickel removal capacity of immobilized Enterobacter sp. cells --- p.37 / Chapter F. --- Effect of bioreactor operational conditions on the Ni2+ removal capacity of immobilized bacterial cells --- p.38 / Chapter G. --- Optimization of nickel removal efficiency of bioreactor --- p.39 / Chapter H. --- Determination of Ni2+ adsorption isotherm of immobilized cells of Enterobacter sp. --- p.39 / Chapter I. --- Recovery of nickel from the bioreactor --- p.40 / Chapter J. --- Activity of the regenerated bioreactor --- p.41 / Chapter K. --- Removal of Ni2+ from synthetic effluent by bioreactor --- p.41 / Chapter L. --- Removal of Ni2+ from electroplating effluent by bioreactor. --- p.41 / Chapter M. --- Production of immobilized bacterial cells by replacement of D-glucose by molasses in the growth medium --- p.42 / Results / Chapter A. --- Isolation and selection of nickel resistant bacteria --- p.44 / Chapter B. --- Effect of growth conditions on nickel removal capacity of immobilized Enterobacter sp. cells / Chapter 1. --- Nutrient limitation --- p.44 / Chapter 2. --- D-glucose concentration --- p.45 / Chapter 3. --- Incubation temperature and incubation time --- p.45 / Chapter C. --- Heavy metal removal capacity of immobilized cells of Enterobacter sp. --- p.50 / Chapter D. --- Effect of bioreactor operational conditions on Ni2+ removal capacity of the immobilized bacterial cells --- p.50 / Chapter E. --- Optimization of nickel removal efficiency of bioreactor --- p.55 / Chapter F. --- Determination of Ni2+ adsorption isotherm of immobilized cells of Enterobacter --- p.57 / Chapter G. --- Recovery of nickel from the bioreactor and activity of regenerated bioreactor against a fresh nickel flow --- p.61 / Chapter H. --- Removal of Ni2+ from synthetic effluent by bioreactor --- p.61 / Chapter I. --- Removal of Ni2+ from electroplating effluent by bioreactor. --- p.64 / Chapter J. --- Production of immobilized bacterial cells by replacement of D-glucose by molasses in the growth medium --- p.68 / Discussions / Chapter A. --- Effect of growth conditions on nickel removal capacity of immobilized Enterobacter sp. cells --- p.70 / Chapter B. --- Heavy metal removal capacity of immobilized cells of Enterobacter sp. --- p.73 / Chapter C. --- Effect of bioreactor operational conditions on Ni2+ removal capacity of the immobilized bacterial cells --- p.74 / Chapter D. --- Optimization of nickel removal efficiency of bioreactor --- p.74 / Chapter E. --- Determination of Ni2+ adsorption isotherm of immobilized cells of Enterobacter sp. --- p.76 / Chapter F. --- Recovery of nickel from the bioreactor and activity of the regenerated bioreactor against a fresh nickel flow --- p.77 / Chapter G. --- Removal of Ni2+ from synthetic effluent by bioreactor --- p.78 / Chapter H. --- Removal of Ni2+ from electroplating effluent by bioreactor --- p.79 / Chapter I. --- Production of immobilized bacterial cells by replacement of D-glucose by molasses in the growth medium --- p.82 / Chapter J. --- Further considerations of applicability of immobilized Enterobacter sp. cells to treatment of electroplating effluent --- p.83 / Conclusions --- p.86 / References --- p.89
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Akkumulering van swaarmetale in 'n myn- en nywerheidsbesoedelde meerekosisteem01 December 2014 (has links)
M.Sc. (Zoology) / Please refer to full text to view abstract
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Die effek van swaarmetale by variërende pH op die bloedfisiologie en metaboliese ensieme van Tilapia sparrmanii (Cichlidae)19 November 2014 (has links)
M.Sc. / Please refer to full text to view abstract
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Heavy metal accumulation in free and immobilized pseudomonas picketti.January 1990 (has links)
by Li Sze Kwan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 234-259. / ACKNOWLEDGEMENT --- p.i / ABSTRACT --- p.ii / CONTENTS : / Chapter CHAPTER 1: --- GENERAL INTRODUCTION --- p.1 / Chapter 1.1 --- Our Environment Is Polluted --- p.1 / Chapter 1.2 --- Heavy Metal Contamination --- p.3 / Chapter 1.3 --- The Effect of Cadmium and Some Related Metals on Environment --- p.5 / Chapter 1.4 --- The Uses of Microorganisms in Cleaning Up Environment --- p.9 / Chapter 1.5 --- Mechanisms of Cadmium Uptake in Cadmium Accumulating Strains --- p.10 / Chapter 1.6 --- Techniques for Cell Immobilization --- p.13 / Chapter 1.7 --- Prospect --- p.20 / Chapter CHAPTER 2: --- ISOLATION OF CADMUIM ACCUMULATNIG MICROORGANISMS --- p.22 / Chapter 2.1 --- Introduction --- p.22 / Chapter 2.2 --- Materials and Methods --- p.25 / Chapter 2.2.1 --- Recipes Used for Growing Various Organisms --- p.25 / Chapter 2.2.2 --- Methods Used for Collecting Organisms to be Tested --- p.27 / Chapter 2.2.3 --- Observation of Samples by Microscope --- p.28 / Chapter 2.2.4 --- Enrichment of Cadmium Resistant Microorganisms --- p.28 / Chapter 2.2.5 --- Selection and Isolation of Cadmium Resistant Microorganisms --- p.29 / Chapter 2.2.6 --- Purification of Microbial Colonies --- p.30 / Chapter 2.2.7 --- Preliminary Classification of Selected Microorganisms --- p.30 / Chapter 2.2.8 --- Screening of Cadmium Accumulating Strains --- p.30 / Chapter 2.2.9 --- Cadmium Analysis --- p.31 / Chapter 2.3 --- Result --- p.32 / Chapter 2.3.1 --- Selection of Cadmium Resistant --- p.32 / Chapter 2.3.2 --- Cadmium Resistance of Isolates --- p.36 / Chapter 2.3.3 --- Screening of Cadmium Accumulating Microorganisms --- p.38 / Chapter 2.4 --- Discussion --- p.39 / Chapter CHAPTER 3: --- GENERAL CHARACTERIZATION OF STRAIN 1000A --- p.43 / Chapter 3.1 --- Introduction --- p.43 / Chapter 3.1.1 --- Various Factors Affecting the Accumulation of Cadmium of Strain 1000A --- p.43 / Chapter 3.1.2 --- Identification --- p.44 / Chapter 3.2 --- Materials and Methods --- p.45 / Chapter 3.2.1 --- "Preparation of Solutions, Antibiotics and Reagents" --- p.45 / Chapter 3.2.2 --- Culture Media Used --- p.47 / Chapter 3.2.3 --- Growth Kenetics Determination --- p.48 / Chapter 3.2.4 --- Determination of the Effect of Cadmium Concentration on Cd-uptake in Free Cells --- p.49 / Chapter 3.2.5 --- Determination of the Effect of Phosphate Concentration on Cd-uptake in Free Cell --- p.49 / Chapter 3.2.6 --- Determination of the Cd-uptake in Free Cells in Continuous Cultures --- p.50 / Chapter 3.2.7 --- Determination of Antibiotic Resistance of Strain 1000A --- p.51 / Chapter 3.2.8 --- Dstermination of Relationship between Chloramphenicol Resistance and Cd-uptake --- p.52 / Chapter 3.2.9 --- Cadmium Analysis --- p.52 / Chapter 3.2.10 --- Determination of Inorganic Precipitation of Cadmium --- p.53 / Chapter 3.2.11 --- Assimilation Tests --- p.54 / Chapter 3.2.12 --- Identification of Strain 1000A --- p.55 / Chapter 3.3 --- Result --- p.55 / Chapter 3.3.1 --- Growth Kinetics of Strain 1000A in Cadmium Supplemented Peptone Medium --- p.55 / Chapter 3.3.2 --- Cd-uptake of Strain 1000A at Various Cadiuin Concentration --- p.65 / Chapter 3.3.3 --- Effect of Phosphate concentration on Cd-uptake of Strain 1000A --- p.65 / Chapter 3.3.4 --- Cd-uptake of Strain 1000A in Continuous Cultures --- p.70 / Chapter 3.3.5 --- Inorganic Precipitation of Cadmium Phosphate --- p.75 / Chapter 3.3.6 --- Determination of Antibiotic-Resistance of Strain 1000A --- p.78 / Chapter 3.3.7 --- Effect of Chloramphenicol on Cd-uptake and Cadmium Resistance of Strain 1000A --- p.82 / Chapter 3.3.8 --- Determination of the Effect of Tetracyclin --- p.85 / Chapter 3.3.9 --- Assimilation Tests --- p.94 / Chapter 3.3.10 --- Identification of Strain 1000A --- p.94 / Chapter 3.4 --- Discussion --- p.97 / Chapter CHAPTER 4: --- DETERMINATION OF CADMIUM UPTAKE MECHANISM IN P. PICKETTI 1000A --- p.102 / Chapter 4.1 --- Introduction --- p.102 / Chapter 4.2 --- Materials and Methods --- p.105 / Chapter 4.2.1 --- Preparation of Solutions and Reagents --- p.105 / Chapter 4.2.2 --- Preparation of Reagents for SDS-PAGE --- p.105 / Chapter 4.2.3 --- Recipes for Growing Cells --- p.107 / Chapter 4.2.4 --- Protein Determination --- p.108 / Chapter 4.2.5 --- Examination of Cadmium Accommodation in P. picketti 1000A by Transmission Electron Microscope --- p.108 / Chapter 4.2.6 --- SDS-polyacrylamide Gel Electrophoretic Determination of Protein Profiles --- p.109 / Chapter 4.2.7 --- Phosphate Assay --- p.111 / Chapter 4.2.8 --- Orthophosphate Estimation --- p.112 / Chapter 4.2.9 --- Sulphide Analysis --- p.112 / Chapter 4.2.10 --- Cadmium Analysis --- p.113 / Chapter 4.2.11 --- Cd-binding Determination through Column Separation --- p.113 / Chapter 4.2.12 --- Cd-binding Determinate ion through SDS Electrophoresis --- p.114 / Chapter 4.2.13 --- Determination of Cadmium Distribution of Cells --- p.115 / Chapter 4.3 --- Result --- p.116 / Chapter 4.3.1 --- SDS-PAGE Determination of Protein Profiles of P. picketti 1000A --- p.116 / Chapter 4.3.2 --- Determination of Cd-binding Protein of P. picketti 1000A --- p.121 / Chapter 4.3.3 --- "Determination of the Relationship of Cellular Cadmium, Sulphide and Phosphate" --- p.131 / Chapter 4.3.4 --- Examination of Cadmium Accumulation of P. picketti 1000A by Transmission Electron Microscope --- p.142 / Chapter 4.3.5 --- Cadmium Distribution of Cadmium-Accommodated Cells --- p.148 / Chapter 4.4 --- Discussion --- p.152 / Chapter CHAPTER 5: --- CORRELATION AMONG METALS IN HEAVY METAL UPTAKE --- p.158 / Chapter 5.1 --- Introduction --- p.158 / Chapter 5.2 --- Materials and Methods --- p.158 / Chapter 5.2.1 --- Preparation of Solutions --- p.159 / Chapter 5.2.2 --- "Determination of Effect of Zn+2 ," --- p.160 / Chapter 5.2.3 --- Determination of Effect of Cu+2 . --- p.161 / Chapter 5.2.4 --- "Correlation among Cd+2, Cu+2 and Zn+2" --- p.161 / Chapter 5.2.5 --- Growth Kenetics Determination --- p.162 / Chapter 5.2.6 --- Cell Sample Preparation --- p.162 / Chapter 5.2.7 --- Orthophosphate Estimation --- p.162 / Chapter 5.2.8 --- Metal Analysis --- p.163 / Chapter 5.3 --- Result --- p.163 / Chapter 5.3.1 --- Effect of Zn+2 --- p.163 / Chapter 5.3.2 --- Effect of Cu+2 --- p.173 / Chapter 5.3.3 --- "Correlation among Cd+2, Cu+2 and Zn+2" --- p.178 / Chapter 5.4 --- Discussion --- p.195 / Chapter CHAPTER 6: --- HEAVY METAL UPTAKE OF IMMOBILIZED CELL --- p.197 / Chapter 6.1 --- Introduction --- p.197 / Chapter 6.2 --- Materials and Methods --- p.199 / Chapter 6.2.1 --- Preparation of Solutions and Medium --- p.199 / Chapter 6.2.2 --- Harvesting of Cells --- p.199 / Chapter 6.2.3 --- Immobilization of Cells --- p.199 / Chapter 6.2.4 --- Determination of the Effect of Temperature --- p.200 / Chapter 6.2.5 --- Determination of Optimum Cell Concentration in Polyacrylamide Gel --- p.201 / Chapter 6.2.6 --- Determination of pH Effect on Cd-uptake --- p.201 / Chapter 6.2.7 --- Pretreatment with 70% Methanol --- p.202 / Chapter 6.2.8 --- Combined Pretreatment with Methanol and NaOH --- p.202 / Chapter 6.2.9 --- Effect of Phosphate on Cd-uptake of Immobilized Cell --- p.202 / Chapter 6.2.10 --- Comparison of Cadmium- and Copper-uptakes in Cells Immobilized in K-carrageenan and in Polyacrylamide --- p.203 / Chapter 6.3 --- Result --- p.204 / Chapter 6.3.1 --- Effect of Temperature on Cd-uptake --- p.204 / Chapter 6.3.2 --- Determination of Optimum Cell Concentration in Polyacrylamide Gel --- p.204 / Chapter 6.3.3 --- Effect of pH on Cd-uptake of Immobilized Cells --- p.207 / Chapter 6.3.4 --- Effect of Methanol on Cd-uptake --- p.210 / Chapter 6.3.5 --- Combined Effect of pH and Methanol on Cd-uptake --- p.213 / Chapter 6.3.6 --- Effect of Phosphate on Cd-uptake of Immobilized Cells --- p.213 / Chapter 6.3.7 --- Comparison between Cadmium- and Copper-uptake of Cells Immobilized in K-carrageenan and in Polyacrylamide --- p.220 / Chapter 6.4 --- Discussion --- p.228 / Chapter CHAPTER 7: --- CONCLUSION --- p.232 / REFERENCES --- p.234
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Heavy metal ion resistance and bioremediation capacities of bacterial strains isolated from an Antimony Mine.Sekhula, Koena Sinah January 2005 (has links)
Thesis (M.Sc.) -- University of Limpopo, 2005 / Six aerobic bacterial strains [GM 10(1), GM 10 (2), GM 14, GM 15, GM 16 and
GM 17] were isolated from an antimony mine in South Africa. Heavy-metal
resistance and biosorptive capacities of the isolates were studied. Three of the
isolates (GM 15, GM 16 and GM 17) showed different degrees of resistance to
antimony and arsenic oxyanions in TYG media. The most resistant isolate GM 16
showed 90 % resistance, followed by GM 17 showing 60 % resistance and GM
15 was least resistant showing 58 % resistance to 80 mM arsenate (AsO4
3-). GM
15 also showed 90 % resistance whereas isolates GM 16 and GM 17 showed 80
% and 45 % resistance respectively to 20 mM antimonate (SbO4
3-). Arsenite
(AsO2
-) was the most toxic oxyanion to all the isolates.
Media composition influenced the degrees of resistance of the isolates to some
divalent metal ions (Zn2+, Ni2+, Co2+, Cu2+ and Cd2+). Higher resistances were
found in MH than in TYG media. All the isolates could tolerate up to 5 mM of the
divalent metal ions in MH media, but in TYG media, they could only survive at
concentrations below 1 mM. Also, from the toxicity studies, high MICs were
observed in MH media than TRIS-buffered mineral salt media. Zn2+ was the most
tolerated metal by all the isolates while Co2+ was toxic to the isolates.
The biosorptive capacities of the isolates were studied in MH medium containing
different concentrations of the metal ions, and the residual metal ions were
determined using atomic absorption spectroscopy. GM 16 was effective in the
removal of Cu2+ and Cd2+ from the contaminated medium. It was capable of
removing 65 % of Cu2+ and 48 % of Cd2+ when the initial concentrations were
100 mg/l, whereas GM 15 was found to be effective in the biosorption of Ni2+
from the aqueous solutions. It was capable of removing 44 % of Ni2+ when the
initial concentration was 50 mg/l. GM 17 could only remove 20 % of Cu2+ or Cd2+.
These observations indicated that GM 16 could be used for bioremediation of
xvi
Cu2+ and Cd2+ ions from Cu2+ and Cd2+-contaminated aqueous environment,
whereas GM 15 could be used for bioremediation of Ni2+. / National Research Foundation and the University of the North Research Unit
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Concept of copper mobility and compatibility with lead and cadmium in landfill linersKaoser, Saleh January 2003 (has links)
No description available.
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Remediation of heavy-metal contaminated soils using succinic acidKaul, Arvind 15 September 1992 (has links)
Succinic acid, a low molecular weight dicarboxylic acid was used to leach out
heavy metals from Willamette Valley soil (contaminated separately with lead, copper,
and zinc) in form of water-soluble organo-metal complexes. The research tasks included
developing synthetic contaminated soils representative of those found at Superfund sites
and making heavy metal adsorption and desorption studies.
Fixed amounts of single-metal contaminated soil were treated with succinic acid
under varying conditions of pH and organic ligand concentration. Based on the total
metal mobilized into the aqueous phase, the optimum values of pH and organic acid were
established for each metal. Since the direct determination of all species solubilized by the
organic acid solution was not possible, a computer speciation program called MICROQL
was used to determine the concentration of metal species in solution containing several
metals and potential ligands.
The results indicate that succinic acid is capable of significantly altering the
partitioning of metals between the soil and the aqueous phase. Higher concentrations of
the organic acid resulted in higher removal of metal from the soil. In case of lead and
copper, low pH (3.5) succinic acid flushing solution was found to be the most effective,
while a pH range of 4.5-5.5 was deemed optimum for zinc. The results also established
that the extent of removal of any metal depended not only upon the the stability constant
of the organo-metal complex, but also on its mode of retention within the soil. / Graduation date: 1993
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Analysis of heavy metals in marine sediments and the determination of heavy metal profiles in dated sediments cores from Sai Kung Bay, HongKongLo, Chi-keung., 盧志強. January 1992 (has links)
published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy
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Bioremediation of soils polluted by heavy metals using organic acidsWasay, Syed A. January 1998 (has links)
Weak organic acids and/or their salts were tested as soil washing or flushing agents for the ex- or in-situ remediation of soils polluted by heavy metals. Three soils naturally with heavy metals were used for the tea. / The three soils were characterized as a clay loam, loam and sandy clay loam. Their organic matter, pH, saturated hydraulic conductivity, cation exchange capacity, particle density and heavy metal contents were also characterized. The different retention forms of heavy metals in all 3 soils were studied by sequential extraction. The clay loam was contaminated with Cr, Hg, Mn and Pb while the loam and sandy clay loam were contaminated with Cd, Pb, Cu and Zn. Weak organic adds and/or their salts and chelating agents (EDTA and DTPA) were used at different pH, levels of concentration and leaching time in batch experiments to establish optimum conditions for maximum removal of heavy metals from the three soils. Citrate and tartarate were found to be quite effective, in leaching heavy metals from these soils. The rate of leaching of heavy metals from soils with citrate, tartarate and EDTA was modeled using two-reaction model at a constant pH and temperature. / Three contaminated soils of different textures were flushed in a column at optimum pH with a salt of weak organic acids, namely, citrate, tartarate, citrate+oxalate or a chelating agent such as EDTA and DTPA. The citrate and tartarate (ammonium salts) were found to be quite effective in removing heavy metals from the three contaminated soils while leaching little macronutrients and improving the soil's structure. An in-situ soil remediation simulation was also successfully tested using the sandy clay loam at large scale level in a tub (plastic container) using citrate as a flushing liquid. EDTA and DTPA were effective in removing the heavy metals except for Hg, but these strong chelating agents extracted important quantities of macronutrients from the soil. These chelating agents are also known to pollute the soil by being adsorbed on the soil particles. / A bioremediation process was developed using the fungus Aspergillus niger to produce weak organic acids (mainly citrate and partly oxalate depending on pH) for the leaching of heavy metals from contaminated soils. The fungus was cultivated on the surface of the three contaminated soils for 15 days at 30°C and a pH ≤ 4 to enhance the production of citric acid rather than oxalic acid which hinders Pb leaching. By extrapolating the result, the three contaminated soils were expected to be sufficiently remediated to meet the A category (Quebec clean up criteria for cleaning soils contaminated by heavy metals) after 20 to 25 days of leaching using this technique. / Finally, the leachate, collected following the soil remediation using weak organic acids and/or their salts, EDTA and DTPA was treated effectively using granular activated carbon.
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Recycling of complexometric extractant(s) to remediate a soil contaminated with heavy metalsLee, Chia Chi January 2002 (has links)
A possible remediation strategy that involved washing with complexing reagents(s) [disodium ethylenediaminetetraacetate (Na2EDTA) alone or in combination with bis-(2-hydroxyethyl)dithiocarbamate (HEDC)] was evaluated with an urban soil that had been field contaminated with excesses of heavy metal (HMs). Heavy metals (Cd, Cu, Mn, Ni, Pb and Zn) were targeted for removal. The aqueous solution that resulted from, washing was treated with zero-valent (ZV) magnesium (Mg0) or bimetallic mixture (Pd0/Mg 0 or Ag0/Mg0) to release the chelating reagent(s) from their heavy metal complexes. During this reaction, the heavy metals were precipitated from solution as hydroxides or became plated on to the surface of the excess ZV reagent. Thus, an appreciable fraction of the mobilized Pb and Cu and a portion of Zn became cemented to the surface of the ZV metal whereas most of the Fe and Mn were removed from solution as insoluble hydroxides. After filtration and pH re-adjustment, the demetallized solution was then returned to the soil to extract more heavy metals. After three washing cycles with the same reagent, it was observed that the sparing quantity of EDTA (10 mmoles) had mobilized 32--54% of the soil burden heavy metals (5 mmoles), but only 0.1% of the iron had been removed. / A 1:1 (mol/mol) mixture of EDTA and HEDC proved to be approximately equally efficient at HM extraction despite more than a three-fold reduction (3 mmoles) in the quantity of reagents. Three washing with the same reagent mobilized some 49% of the Pb, 18% of the Zn and 19% of the Mn but only 7% of the Cu and 1% of the Fe from the test soil.
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