Tourism was realized very rarely a few decades ago. Today it is a significant part of the world's GDP and has become a major economic factor. The Czech Republic is a very attractive country in the heart of Europe with numerous natural, historical and cultural values. The tourist region Vysočina can boast all these qualities, yet one of the least visited region in the Czech Republic. This thesis deals with a detailed analysis of the potential of the tourist region Vysočina to become a competitive tourist destination. The aim of this work is to analyze primary and secondary tourism field and to assess the organizational structure of tourism and promotional activities of tourism operators with an emphasis on planned projects. The author's own research, focused on the general opinion on the issue of tourism in the region and on the evaluation of the tourism offer, is a part of this work as well. The benefit of the thesis lies in the overall recovery of the tourist supply and in making the region more attractive which can contribute to potential subsequent increased and mobilizing demand for tourism in Vysočina.